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MicroRNA-7641 as a Promising Targeting Factor to Improve the Efficacy of Cancer Therapy
Abu Musa Md Talimur Reza,Jae woong Han,Yun-jung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Many diseases, including myocardial infarction, autoimmune disease, viral diseases, neurodegenerative diseases, and cancers, are frequently diagnosed with the aberrant expression of microRNAs (miRNAs) and their allied pathways. This indicates the crucial role of miRNAs in maintaining biological and physiological processes. miR-7641 is a miRNA whose role in disease has not been fully investigated. In the present study, we investigated the expression pattern of miR-7641 and its target genes in different cancer cells, as well as in patients with breast cancer and colorectal cancer. Direct inhibition of miR-7641 using a locked nucleic acid upregulated the expression of its target genes, sensitized cancer cells, and enhanced the efficiency of therapeutic agents such as doxorubicin. In addition, inhibition of miR-7641 boosted doxorubicin-mediated apoptosis of cancer cells via upregulation of apoptotic molecules Caspase 9 (CAS9) and poly ADP ribose polymerase (PARP) and downregulation of anti-apoptotic molecule BCL2. Thus, these findings indicated that miR-7641 is a clinically important therapeutic target for cancers. Inhibition of miR-7641 expression could be an efficient treatment strategy for patients with breast and colorectal cancer. In addition, inhibitors of miR-7641 could be used in combination with other therapeutic agents, such as doxorubicin, to enhance the efficiency of cancer therapy.
Abu Musa Md Talimur Reza,Jae woong Han,Yun-Jung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Recombination activating gene-2 (Rag2) participate in the immune system process through V(D)J recombination of B-cells and T-cells activation process. The deletion of Rag2 gene impaired the immune regulation through complete loss of matured B-cells and T-cells. However, in this research we found that Rag2-ablated mice showed very marginal expression for both B-cells (CD20 and CD79a) and T-cells (CD4 and CD8) markers. These data indicated the possibility of leakiness of B-cells and T-cells at least in a marginal level in the spleen and thymus of Rag2-deficient mice. It also suggested that effectiveness of Rag2 deletion could be regulated based on the strain and age of the concerned mice. Thereafter, we investigated the miRNAs and genes expression profile of Rag2 knockout mice spleen and found that some of the deregulated genes are putative targets of the differentially expressed miRNAs and might be transcriptionally connected. Moreover, the deregulated miRNAs and genes are also actively involved in both B-cells and T-cells activation signaling network. Finally, we concluded that miRNAs along with the related genes are important regulator of B-cells and T-cells signaling cascade, and the Rag2 deletion might impaired B-cells and T-cells development in a miRNAs-dependent manner.
Jae woong Han,Abu Musa Md Talimur Reza,Yun-Jung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Silver nanoparticles (AgNPs) is one of the nanomaterials as most popular and commonly used in consumer products in biomedical devices, antibiotic agent, wound dressings, text tile and also candidate of anti-cancer material. F9 cell, the teratocarcinoma stem cell line has been widely used as a model for the analysis of molecular mechanisms associated with differentiation and retinoic acid (R.A) is well known molecule that induce F9 cell differentiation. There are many study about induce differentiation in stem cells by AgNPs and in anti-cancer therapy, induce cancer stem cell differentiation is very important to killing cancer. In this study, we demonstrated that AgNPs showing toxicity in F9 cells as anti-cancer cell effect and also induce F9 cell differentiation. F9 cells are induce cell toxicity and apoptosis after treated AgNPs and also make F9 cell differentiation like induced by R.A. Signaling pathway analysis showing that gene and protein expression going different and more sensetive to anti cancer drug, cisplatin, after induced differentiation by AgNPs. We found that potential of AgNPs as not only killing cancer but also make cancer stem cell going to differentiation.
Graphene Oxide Size Dependent Toxicity to TM3, TM4 and Spermatogonial Stem Cell
Jae woong Han,Abu Musa Md Talimur Reza,Yun-Jung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Graphene oxide (GO) is a novel material derived from two-dimentional carbon lattice of graphene and has attracted increasing interest in the field of biomedicine as grug delivery, gene delivery, biosensers. Also G.O is well known that low toxicity in cells but effect in germ cell development are poorly understood. Leydig cells and Sertoli cells are somatic cells that surpport germ cells during spermatogenesis. In this study, we found that toxicity to leydig and sertoli cell line induced by G.O following variants size. The small size (about 27 nm) showing more high toxicity and cell damage, apoptosis in those somatic cells compare with large size (about 80 nm). Small size G.O is more easy to upkate to cells and effect to cells. Taken together, our study reveals that G.O showing cell toxicity, even if low, and possibility that interrupt spermatogenesis by damage of leydig and sertoli cells. It is important information that apply small size of G.O as biomeical drug.