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( Takamasa Ishii ),( Masaki Miyazawa ),( Phil S. Hartman ),( Naoaki Ishii ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.5
Most intracellular reactive oxygen species (ROS), especially superoxide anion (O2·-) that is converted from oxygen, are overproduced by excessive electron leakage from the mitochondrial respiratory chain. Intracellular oxidative stress that damages cellular components can contribute to lifestyle-related diseases such as diabetes and arteriosclerosis, and age-related diseases such as cancer and neuronal degenerative diseases. We have previously demonstrated that the excessive mitochondrial O2·- production caused by SDHC mutations (G71E in C. elegans, I71E in Drosophila and V69E in mouse) results in premature death in C. elegans and Drosophila, cancer in mouse embryonic fibroblast cells and infertility in transgenic mice. SDHC is a subunit of mitochondrial complex II. In humans, it has been reported that mutations in SDHB, SDHC or SDHD often result in inherited head and neck paragangliomas (PGLs). Recently, we established Tet-mev-1 conditional transgenic mice using our uniquely developed Tet-On/Off system, which equilibrates transgene expression to endogenous levels. These mice experienced mitochondrial respiratory chain dysfunction that resulted in O2·- overproduction. The mitochondrial oxidative stress caused excessive apoptosis leading to low birth weight and growth retardation in the neonatal developmental phase in Tet-mev-1 mice. Here, we briefly describe the relationships between mitochondrial O2·- and aging phenomena in mev-1 animal models. [BMB reports 2011; 44(5): 298-305]
Takamasa Takahashi,Yuji Kaneoka,Atsuyuki Maeda,Yuichi Takayama,Hiroki Aoyama,Takahiro Hosoi,Kazuaki Seita 대한내시경로봇외과학회 2023 Journal of Minimally Invasive Surgery Vol.26 No.2
Purpose: In minimally invasive esophagectomy (MIE), it is important to reduce the rate of anastomotic leakage to ensure its safety. At our institute, the double-ligation method (DLM) has been introduced to insert and fix the anvil of the circular stapler for intracorporeal circular esophagojejunostomy in gastric surgery. We adopted this method for intrathoracic anastomosis (IA) in MIE. The aim of this study was to investigate the safety of IA with DLM in MIE. Methods: In this study, 48 patients diagnosed with primary middle or lower third segment thoracic esophageal carcinoma with clinical stage I, II, III or IV disease were retrospectively evaluated. Postoperative outcomes were assessed. Results: Among the 48 patients, 42 patients underwent laparo-thoracoscopic esophagectomy and IA using a circular stapler with the DLM. The average total operation time and thoracoscopic operation time were 433 and 229 minutes, respectively. The average pursestring suturing time was 4.7 minutes. The rates of anastomotic leakage and stenosis were 2.4% and 14.3%, respectively. The overall incidence of postoperative complications (Clavien- Dindo grade of ≥III) was 16.7%. The average postoperative stay was 16 days. Conclusion: The procedure of IA using a circular stapler with the DLM in MIE was safe and provided a low rate of anastomotic leakage.
Numano, Takamasa,Xu, Jiegou,Futakuchi, Mitsuru,Fukamachi, Katsumi,Alexander, David B.,Furukawa, Fumio,Kanno, Jun,Hirose, Akihiko,Tsuda, Hiroyuki,Suzui, Masumi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2
Two types of nanosized titanium dioxide, anatase ($anTiO_2$) and rutile ($rnTiO_2$), are widely used in industry, commercial products and biosystems. $TiO_2$ has been evaluated as a Group 2B carcinogen. Previous reports indicated that $anTiO_2$ is less toxic than $rnTiO_2$, however, under ultraviolet irradiation $anTiO_2$ is more toxic than $rnTiO_2$ in vitro because of differences in their crystal structures. In the present study, we compared the in vivo and in vitro toxic effects induced by $anTiO_2$ and $rnTiO_2$. Female SD rats were treated with $500{\mu}g/ml$ of $anTiO_2$ or $rnTiO_2$ suspensions by intra-pulmonary spraying 8 times over a two week period. In the lung, treatment with $anTiO_2$ or $rnTiO_2$ increased alveolar macrophage numbers and levels of 8-hydroxydeoxyguanosine (8-OHdG); these increases tended to be lower in the $anTiO_2$ treated group compared to the $rnTiO_2$ treated group. Expression of $MIP1{\alpha}$ mRNA and protein in lung tissues treated with $anTiO_2$ and $rnTiO_2$ was also significantly up-regulated, with $MIP1{\alpha}$ mRNA and protein expression significantly lower in the $anTiO_2$ group than in the $rnTiO_2$ group. In cell culture of primary alveolar macrophages (PAM) treated with $anTiO_2$ and $rnTiO_2$, expression of $MIP1{\alpha}$ mRNA in the PAM and protein in the culture media was significantly higher than in control cultures. Similarly to the in vivo results, $MIP1{\alpha}$ mRNA and protein expression was significantly lower in the $anTiO_2$ treated cultures compared to the $rnTiO_2$ treated cultures. Furthermore, conditioned cell culture media from PAM cultures treated with $anTiO_2$ had less effect on A549 cell proliferation compared to conditioned media from cultures treated with $rnTiO_2$. However, no significant difference was found in the toxicological effects on cell viability of ultra violet irradiated $anTiO_2$ and $rnTiO_2$. In conclusion, our results indicate that $anTiO_2$ is less potent in induction of alveolar macrophage infiltration, 8-OHdG and $MIP1{\alpha}$ expression in the lung, and growth stimulation of A549 cells in vitro than $rnTiO_2$.
古賀崇雅 ( Koga Takamasa ) 퇴계학부산연구원 2017 退溪學論叢 Vol.30 No.-
李退溪の『自省錄』のなかの「心氣の患い」という言葉に注目して、退溪學における學問とは、生涯を通じてなされるべき事業であり、もし氣質の變化に成功したならば、それは、ほとんど人生の一大歡喜の事であるとされたことを明らかにし、つぎに、『自省錄』に見られる强い自己反省にもとづく禁欲的な退溪の學問が門人たちにどのように傳えられたのかを趙月川と李德弘の場合をとりあげた。 そして、日本の幕末維新期の崎門學者である楠本碩水の氣質の變化について述べ、そこに生涯を通じた退溪學の受容をみた。 それは、つまり、退溪の學問は時間と空間を越えて受容されたことを意味した。 I focused on the word “Sickness of Mind” in Lee Toegye (李退溪)’s “Self-examination proceedings (自省錄),” and I found that the Scholarship of Li Tui Xi (Lee Toegye (李退溪)) is a project worth researching throughout our lifetime. I cleared that it was recognized as a great joy of life if temperament change had been succeeded. And then, I took up the cases of Zhao Yue Chuan (趙月川) and Lee T. (李德弘), and I showed how the ascetic Scholarship of Li Tui Xi based on strong self-reflection as seen in “Self-examination proceedings (自省錄)” was conveyed to the students. I described about the change of temperament of Kusumoto Sekisui who was a scholar of Kimon (崎門學) at the end of Edo period in Japan and I found his acceptance of the Scholarship of Li Tui Xi throughout his life. In other words, it meant that Toegye’s Teachings were accepted beyond time and space.
Takako Enokida,Takamasa Noda,Takashi Usami,Naoko Satake,Kazuyuki Nakagome 대한정신약물학회 2022 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.20 No.1
Pharmacotherapy is generally the first choice for the treatment of acute mania in bipolar disorder. Electroconvulsive therapy (ECT) is reported to be an effective treatment modality for mania; however, it is usually used as the “last resort.” Herein, we report a case of a patient with treatment-resistant severe mania in bipolar disorder who recovered with ECT without concurrent antipsychotics and mood stabilizers. Our case report showed that ECT monotherapy can be an effective treatment modality for manic state in bipolar disorder, which may lead to a shorter hospital stay and better social outcomes.
Manabu Akahane,Takamasa Shimizu,Yusuke Inagaki,Tsutomu Kira,Takuya Egawa,Akinori Okuda,Tadanobu Onishi,Tomoaki Imamura,Yasuhito Tanaka 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.1
The purpose of this study was to evaluate the osteogenesis ability of osteogenic matrix cell sheets (OMCS) derived from old donor cells. Bone marrow stromal cells (BMSC) were obtained from young (7-week-old) and old (1-yearold) Fischer344 rats donors and cultured with modified Eagle’s medium (MEM group) alone or containing dexamethasone (Dex; 10 nM) and ascorbic acid phosphate (AscP; 0.28 mM) (Dex/AscP group). We prepared four in vitro experimental groups: (1) young MEM, (2) young Dex/AscP, (3) old MEM and (4) old Dex/AscP. Cell proliferation and osteogenic marker mRNA expression levels were evaluated in vitro. To assess bone formation in vivo, the cells of each group were combined with beta tricalcium phosphate (TCP) disks followed by implantation in recipient rats. The in vitro study showed significant differences in the mRNA expression of osteocalcin, ALP, and BMP2 between MEM and Dex/AscP groups. Bone formation following implantation was observed upon histological analyses of all groups. TCP combined with OMCS (OMCS/TCP group) resulted in enhanced bone formation compared to that following combination with BMSC (BMSC/ TCP). The osteocalcin content of the OMCS/TCP group 4 weeks after implantation was significantly higher than that in the BMSC/TCP construct for both young and old donors. The present study clearly indicated that OMCS could be generated from BMSCs of old as well as young donors using a mechanical retrieval method. Thus, through its usage of OMCS, this method may represent a potentially effective therapeutic option for cell-based therapy in elderly patients.