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Bis-Diazotized Benzidine에 의한 연속 및 동시흡착법에 의하여 Tuberculin PPD로 감작된 적혈구를 이용한 혈구응집반응의 민감성
김택선,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1984 中央醫大誌 Vol.9 No.3
Passive haemagglutination test(PHA) using sheep red blood cells(SRBC) sensitized with soluble antigens by means of coupling agents, i.e., tannic acid, bis-diazotized benzidine(BDB), chromic chloride, glutaraldehyde, and so on, provide an extremely sensitive technique to detect a minute quantity of serum antibody as low as 0.01㎍. It also has advantage over agglutination and precipitation tests in that if it is transferred either to hemolysis test(PHL) or to inhibition test(PHI), soluble antigen-antibody complexes and incomplete or hapten antigen-nonprecipitating antibody complexes that are not detectable by other tests can be demonstrated. This study was undertaken to determine the optimum amount of BDB and tuberculin PPD antigen for consecutive and simultaneous coupling of SRBC in conjunction with the sensitivity of the PHA test. In addition, the sensitivities of PHA and PHL test were compared and the PHA units of antibody and diluents for tuberculin PPD antigen for PHI test were discussed. The results are summarized as follows: 1. The optimum amount of BDB for consecutive and simultaneous coupling of 0.2ml of 2.5% SRBC were 20㎍ and 13㎍, respectively, and the optimum amount of tuberculin PPD antigen were about 125㎍ and 250㎍ respectively. The optimum ratios between BDB and tuberculin PPD antigen for consecutive and simultaneous coupling were 1:6.3 and 1:19.2, respectively. 2. In the consecutive coupling, the PHA titre was linear to both BDB and tuberculin PPD concentrations, whereas in simultaneous coupling it was affected by the level of tuberculin PPD but not by that of BDB from 5㎍ to 13㎍. 3. Using SRBC sensitized consecutively with tuberculin PPD or sensitins prepared from four species of mycobacteria the homologous PHL tests were sensitive than the PHA tests by 1:2 to 1:4 dilutions, and the end-point of PHL test was more clear-cut than that of PHA test.
Wegener 씨 육아종 증례보고 1예 : A Case Report
정선관,임형근,정은택,노병석,김창근,원종진 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2
Wegener's granulomatosis is characterized by necrotizing and granulomatous disease which involves upper, lower respiratory tract and kidney. The basic pathophysiologic mechanism of Wegener's granulomatosis is still unknown. We report a case of Wegener's granulomatosis which involved lung and pleura and was diagnosed by clinical symptoms, radiologic findings and anti-neutrophilic cytoplasmic autoantibodies.
Formocresol과 水酸化 Calcium 및 Glutaraldehyde가 切斷施術된 齒髓에 미치는 影響에 關한 實驗的 硏究
吳世潤,韓澤善 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1
This experimental study was performed to compare the effects of formocresol, calcium hydroxide and glutaraldehyde on the vitally amputated pulp tissues. 8 premolars of each sample, total of 64 from 8 young sound dogs were pulpotomized with one of the following 8 different methods. i.e., 5 minutes application of formocresol on the amputated pulp surface and capping with zinc oxide eugenol paste mixed with formocresol, 5 minutes application of formocresol and capping with calcium hydroxide mixed with or without formocresol, 4 days application of formocresol and capping with either zinc oxide eugenol paste or calcium hydroxide, direct capping with calcium hydroxide only or calcium hydroxide mixed with formocresol, and 5 minutes application of 2% glutaraldehyde and capping with calcium hydroxide mixed with 2% glutaraldehyde. 3 dogs were sacrificed 3 weeks later and the other 5 were sacrificed 200 day later respectively, and all the pulpotomized teeth were prepared for histological findings. The obtained results were as follows; 1. In the pulp tissues that were treated with formocresol and capped with zinc oxide engenol mixed with or without formocresol, no dentin bridge was observed and inflammatory response seemed to progress deeper leading to necrosis of the pulp sooner or later. 2. In the pulp tissues that were treated with formocresol for 5 minutes and capped with calcium hydroxide mixed with or without formocresol, or directly capped with calcium hydroxide mixed with formocresol, relatively mild inflammatory reaction was observed with complete dentin bridge formation and the vitality of the remaining pulp tissues appeared to be slightly degenerated. 3. In the pulp tissues that were treated with formocresol for 4 days, generally degenerative and destructive change was severer than in the ones that were treated for 5 minutes. 4. In the pulp tissues that were capped with calcium hydroxide, more favorable tissue reaction was observed when used with formocresol than in the ones where formocresol was not used. 5. In the pulp tissues that were treated with 2% glutaraldehyde and capped with calcium hydroxide mixed with 2% glutaraldehyde instead of formocresol, generally compact dentin bridge was formed and the vitality of the remaining pulp tissues was preserved for a long time relatively with less inflammatory response. 6. In the early stage of osseous structure formation, it appeared relatively spongeous structure showing many spaces, forming tunnels between capping material and the remaining pulp. In the favorable cases, the space was more rapidly closed and dentin bridge was densely added along the lower border of osseous structure covering the remaining pulp, on the other hand, as the formation of dense dentin bridge was retarded, it was more spacious and thus the inflammatory stimulus penetrating deeper and destroying the remaining pulp more severely. 7. The vitality of remaining pulp tissue, pulpotomized with most of the usual methods, seemed to be declined so the author suppose that further more improved method would be necessary.