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축산 폐수의 효율적 처리를 위한 광합성 미세조류인 Spirulina platensis 배양 공정의 최적화
안주희,김성수,김태호,이준엽,오상집,이진하,이현용 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
돈분 폐수의 효율적인 처리를 위해 6 W/㎡, 12 W/㎡와 24 W/㎡의 조도 하에서 온도를 15℃에서 40℃까지 각각 변화시켜, 광합성 미세 조류인 Spirulina platensis의 생육에 관한 최적 생육 조건 결정에 관한 실험을 하였다. 균체의 비생육속도는 30℃까지는 온도 증가에 따라 증가하였다. 균체의 생육에 있어서 온도의 영향을 설명하기 위해서 Arrhenius 식을 이용하여서 얻은 활성화 에너지 값은 13.5kal/mol이었다. 순수 배지에서 배양된 균체의 비생육속도가 0.31(1/day)인 반면 30% 돈분 첨가 배양시 얻은 균체의 비생육속도는 0.24(1/day)였다. 폐수내의 총 질소량과 이의 제거속도로 균체의 폐수 처리 능력을 평가할 수 있었다. 돈분 농도를 달리 첨가한 회분배양에 있어서 70~93%(PO_4^3- -P), 67~93%(inorganic nitrogen), 80~90% (COD) 그리고 37~56%(organic nitrogen)의 감소율을 보였다. 총-질소와 총-인의 1차반응 감소 속도 상수는 각각 0.17(1/day)와 0.14(1/day)로 계산되었다. 20% 돈분을 첨가하는 연속배양에 있어서 회석률이 0.20(1/day)일 때 최대 균체농도가 1.52(g/L)를 유지했다. 돈분처리 후 얻은 균체의 성분은 단백질이 58.7%, 지방 11.0% 그리고 회분이 15.6%였다. The kinetics of growing microalga, Spirulina platensis was investigated to treat swine wastes with optimum growth conditions. Temperature was varied from 15 to 40℃ at three different light intensities, 6 W/㎡, 12 W/㎡ and 24 W/㎡. The specific growth rate was increased as temperature increased up to 30℃. The activation energy was estimated as 13.5 kcal/mol by an Arrhenius relationship. 0.24(1/day) of specific growth rate was obtained from batch cultivation with 30% swine wastes, compared to 0.31 (1/day) from clean culture. It was found that Spirulina platensis was able to reduce 70~93% of PO_4^3- -P, 67~93% of inorganic nitrogen, 80~90% of COD and 37~56% organic nitrogen by adding various concentrations of swine wastes for 12 days of batch cultivation. Rate constants for removing nitrates and phosphates in treating swine wastes were estimated as 0.17 (1/day) and 0.14 (1/day) in the first order reaction, respectively. 1.52(g/L) of maximum cell density was maintained at 0.20(1/day) of dilution rate in continuous culture, adding 20% swine wastes for 30 days. The chemical composition of the biomass obtained from the process showed 58.7% of protein, 11.0% of lipid and 15.6% of ash.
Tae Hyeon Park,Chang-Yun Choi,Jeong-Rok Song,Damee Park,Tae-Jip Kim 한국당과학회 2021 한국당과학회 학술대회 Vol.2021 No.01
From the genome of Lactobacillus shenzhenensis, the probable genes encoding five α -L-arabinofuranosidases (AF, EC3.2.1.55) and an β-L-arabinopyranosidase (AP, EC3.2.1.88) were found and cloned in Escherichia coli. Except LbshAF-B1, five genes were functionally expressed and characterized in this study. The comparative studies on the hydrolytic modes of action revealed that these exo-hydrolases can play the important roles in the enzymatic saccharification of arabinan polymers into L-arabinose. LbshAF-A GH51 could hydrolyze both α-(1,5)- and α-(1,2)/(1,3)-linkages in arabinan. LbshAF-B2 GH51 showed only α-(1,2)/(1,3)-specific debranching activity to generate linear products, whereas LbshAF-C1 and C2 GH43 could exclusively hydrolyze α-(1,5)-linked arabinan backbone. LbshAP showed the activity against p-nitrophenyl arabinopyranoside. It was proposed that the synergistic actions of these exo-hydrolases can facilitate the complete degradation of sugar beet arabinan to L-arabinose.
( Tae Hyeon Park ),( Chang-yun Choi ),( Hyeon Jin Kim ),( Jeong-rok Song ),( Damee Park ),( Hyun Ah Kang ),( Tae-jip Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.2
Two genes encoding probable α-L-arabinofuranosidase (E.C. 3.2.1.55) isozymes (ABFs) with 92.3% amino acid sequence identity, ABF51A and ABF51B, were found from chromosomes 3 and 5 of Saccharomycopsis fibuligera KJJ81, an amylolytic yeast isolated from Korean wheat-based nuruk, respectively. Each open reading frame consists of 1,551 nucleotides and encodes a protein of 517 amino acids with the molecular mass of approximately 59 kDa. These isozymes share approximately 49% amino acid sequence identity with eukaryotic ABFs from filamentous fungi. The corresponding genes were cloned, functionally expressed, and purified from Escherichia coli. SfABF51A and SfABF51B showed the highest activities on p-nitrophenyl arabinofuranoside at 40~45oC and pH 7.0 in sodium phosphate buffer and at 50oC and pH 6.0 in sodium acetate buffer, respectively. These exoacting enzymes belonging to the glycoside hydrolase (GH) family 51 could hydrolyze arabinoxylooligosaccharides (AXOS) and arabino-oligosaccharides (AOS) to produce only L-arabinose, whereas they could hardly degrade any polymeric substrates including arabinans and arabinoxylans. The detailed product analyses revealed that both SfABF51 isozymes can catalyze the versatile hydrolysis of α-(1,2)- and α-(1,3)-L-arabinofuranosidic linkages of AXOS, and α-(1,2)-, α-(1,3)-, and α-(1,5)- linkages of linear and branched AOS. On the contrary, they have much lower activity against the α- (1,2)- and α-(1,3)-double-substituted substrates than the single-substituted ones. These hydrolases could potentially play important roles in the degradation and utilization of hemicellulosic biomass by S. fibuligera.
A New Waxy Corn Hybrid Cultivar, "Ilmichal" with Good Eating Quality and Lodging Resistance
Tae Wook Jung,Hyeon Gui Moon,Beom Young Son,Si Ju Kim,Seon Woo Cha,Hwang Kee Min,Hong Jip Choi,In Mo Ryu 한국육종학회 2006 한국육종학회지 Vol.38 No.2
A single cross, Ilmichal, is a waxy corn hybrid (Zea mays L.) developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2005. This hybrid, which has white kernels, a good eating quality, lodging resistance and high yiel