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TRANHONG QUANG,이동성,Se Jong Han,Il Chan Kim,임정한,김윤철,오현철 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.8
Chemical investigation on the methanol extract of the starfish Ctenodiscus crispatus resulted in the isolation of five steroids, (22E,24ξ)-26,27-bisnor-24-methyl-5α-cholest-22-en-3β,5,6β,15α,25-pentol 25-O-sulfate (1), (22E,24R,25R)-24-methyl-5α-cholest-22-en-3β,5,6β,15α,25,26-hexol 26-O-sulfate (2), (28R)-24-ethyl-5α- cholesta-3β,5,6β,8,15α,28,29-heptaol-24-sulfate (3), (25S)-5α-cholestane-3β,5,6β,15α,16β,26-hexaol (4), and Δ7-sitosterol (5). Their structures were identified by extensive spectroscopic analyses, including 1D, 2D NMR and MS and chemical methods. Compound 4 showed cytotoxicity against human hepatoma HepG2 and glioblastoma U87MG cells via inhibition of cell growth and induction of apoptosis. Induction of apoptosis by 4 was demonstrated by cell death, DNA fragmentation, increased Bax/Bcl-2 protein ratio and the activation of caspase-3, caspase-9 and poly (ADP-ribose) polymerase (PARP).
TRANHONG QUANG,Nguyen Thi Thanh Ngan,Chau Van Minh,Phan Van Kiem,Nguyen Xuan Nhiem,BUIHUU TAI,Nguyen Phuong Thao,Doobyeong Chae,Vivek Bhakta Mathema,고영상,이제현,양서영,김영호 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.3
Kalopanax pictus (Araliaceae) is a deciduous treedistributed in Korea, Japan, and China. The stem bark ofK. pictus has been functionally used as a traditional crude drugfor the treatment of various inflammatory diseases. In thepresent study, we describe the inhibitory effects of oleananetypetriterpenes and saponins isolated from the stem bark ofK. pictus on production of pro-inflammatory cytokines inLPS-stimulated bone marrow-derived dendritic cells. Of thecompounds tested, 16,23,29-trihydroxy-3-oxo-olean-12-en-28-oic acid (1), 4,23,29-trihydroxy-3,4-seco-olean-12-en-3-oate-28-oic acid (2), 3b,6b,23-trihydroxyolean-12-en-28-oicacid 28-O-b-D-glucopyranoside (3), nipponogenin E (6),3b,6b,23-trihydroxyolean-12-en-28-oic acid (7), and caulophyllogenin(19) significantly inhibited the production ofIL-12 p40 and IL-6 with IC50 values ranging from 3.3 to 9.1 lM. Compounds 2, 3, 7, and 19 significantly suppressedthe secretion ofTNF-a with IC50 ranging from8.8 to 20.0 lM. These data provide scientific support for the use of K. pictusstem bark and its triterpene and saponin components in theinhibition of pro-inflammatory cytokine secretion, includingIL-12 p40, IL-6, and TNF-a, and for prevention and treatmentof inflammatory diseases.
윤형문,박경란,TRANHONG QUANG,오현철,홍진태,김윤철,김은철 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.5
This study was performed to examine the anticancerand anti-metastatic effects of 4-parvifuran (PVN), anovel flavonoid isolated from the heartwood of Dalbergiaodorifera, and to study its underlying signaling pathway inhuman osteosarcoma cells. In the present study, PVN wasfound to inhibit cell proliferation in a concentration- andtime-dependent manner in the human osteosarcoma celllines studied (MG-63 and U-2 OS) and induce apoptosis, asevidenced by Annexin V? and TUNEL? cells. Cleavedpoly (ADP-ribose) polymerase (PARP) and caspase-3 wereup-regulated while anti-apoptotic proteins including Bcl-2,Bcl-xL, and survivin were down-regulated after treatmentwith PVN. Matrigel cell migration assay, invasion assay,and soft agar assay were used to show that PVN effectivelysuppressed cell migration and invasion and colony formationin osteosarcoma cells. Protein and mRNA levels ofMMP-2 and MMP-9 were reduced by PVN in a concentration-dependent manner. Furthermore, PVN inhibitedJanus kinase 2 (JAK2)/signal transducers and activators oftranscription 3 (STAT3), mitogen-activated protein kinases(MAPKs) including JNK, ERK, p38 kinase, and cAMPresponse element-binding protein (CREB). Therefore, thisis the first study to demonstrate that PVN might be a novelanticancer and anti-metastatic agent for the treatment ofosteosarcoma through the inhibition of JAK2/STAT3,MAPKs, and CREB signaling pathways.
Nguyen Thi Thanh Ngan,TRANHONG QUANG,김관우,김혜진,손재학,강대길,이호섭,김윤철,오현철 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.3
After the chemical investigation of the ethylacetate extract of the marine-derived fungal strain Penicilliumsp. SF-5629, the isolation and structural elucidationof eight secondary metabolites, including (3R,4S)-6,8-dihydroxy-3,4,7-trimethylisocoumarin (1), (3S,4S)-sclerotininA (2), penicitrinone A (3), citrinin H1 (4), emodin (5),x-hydroxyemodin (6), 8-hydroxy-6-methyl-9-oxo-9Hxanthene-1-carboxylate (7), and 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (8) were carried out. Evaluation of the anti-inflammatory activity of thesemetabolites showed that 4 inhibited nitric oxide and prostaglandinE2 production in lipopolysaccharide-stimulatedBV2 microglia, with IC50 values of 8.1 ± 1.9 and8.0 ± 2.8 lM, respectively. The inhibitory function of 4was confirmed based on decreases in inducible nitric oxidesynthesis and cyclooxygenase-2 gene expression. In addition,4 was found to suppress the phosphorylation ofinhibitor kappa B-a, interrupt the nuclear translocation ofnuclear factor kappa B, and decrease the activation of p38mitogen-activated protein kinase.
Nguyen Huu Tung,김영호,TRANHONG QUANG,Jeong-Hyun Son,구정은,Hye-Jin Hong,고영상,Gyu Yong Song 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4
Interleukin-12, a heterodimeric cytokine comprising p40 and p35 subunits, plays an essential role in the regulating the differentiation of Th cells, which establish and maximize the capabilities of the immune system. The aim of present study is to screen the effect of 21 ginsenosides from steamed ginseng-leaves and flowers on IL-12 production in bone marrow-derived dendritic cells induced by lipopolysaccharide. Noticeably, ginsenoside Rg_6 (12) and ginsenoside F_4 (13) exhibited particularly inhibitory effect on LPS-induced IL-12 production with the inhibition values of 80 and 82%; and ginsenoside ST1 (4), ginsenoside SL_2 (8), ginsenoside SL_3 (9),ginsenoside Rh_3 (14), ginsenoside Rk_2 (15), and ginsenoside Rs_4 (18) showed moderate effects with inhibition rates of 63, 65, 67, 68, 71, 73, and 67%, respectively. These results warrant further studies concerning potential of saponin extracts of steamed ginseng-leaves and flowers for medicinal uses.
Chu Van Men,강종성,장유선,Kwan Jun Lee,이제현,TRANHONG QUANG,Nguyen Van Long,Hoang Van Luong,김영호 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12
A quantitative and pattern recognition analyses were conducted for quality evaluation of Kalopanacis Cortex (KC) using HPLC. For quantitative analysis, four bioactive compounds, liriodendrin, pinoresinol O-β-D-glucopyranoside, acanthoside B and kalopanaxin B, were determined. The analysis method was optimized and validated using ODS column with mobile phase of methanol and aqueous phosphoric acid. The validation gave acceptable linearities (r > 0.9995), recoveries (98.4% to 101.9%) and precisions (RSD < 2.20). The limit of detection of compounds ranged from 0.4 to 0.9 μg/mL. Among the four compounds, liriodendrin was recommended as a marker compound for the quality control of KC. The pattern analysis was successfully carried out by analyzing thirty two samples from four species, and the authentic KC samples were completely discriminated from other inauthentic species by linear discriminant analysis. The results indicated that the method was suitable for the quantitative analysis of liriodendrin and the quality evaluation of KC.
Nguyen Xuan Nhiem,Pham Hai Yen,Nguyen Thi Thanh Ngan,TRANHONG QUANG,Phan Van Kiem,Chau Van Minh,Bui Huu Tai,Nguyen Xuan Cuong,Seok Bean Song,김영호 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.4
Momordica charantia is used to treat various diseases, including inflammatory conditions. Previous reports indicated that the extract of this plant inhibits activation of nuclear transcription factor-jB (NF-jB) but activates peroxisome proliferator-activated receptor (PPAR). Additionally, cucurbitane-type triterpene glycosides are the main bioactive components of the fruit of M. charantia. Therefore, we investigated the anti-inflammatory activity of 17 cucurbitane-type triterpene glycosides (1–17) isolated from this plant. Their inhibition of NF-jB and activation of PPAR activities in HepG2 cells were measured using luciferase reporter and PPAR subtype transactivation assays. Compounds 6 and 8 were found to inhibit NF-jB activation stimulated by tumor necrosis factor-a (TNFa) in a dose-dependent manner. With 50% inhibition concentration (IC50) values of 0.4 lM, compounds 6 and 8 were more potent inhibitors than the positive control, sulfasalazine (IC50 = 0.9lM). Compounds 4, 6, and 8 also inhibited TNFa-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2mRNA. However, only compound 13 significantly increased PPARc transactivation.
Phan Van Kiem,김영호,Chau Van Minh,Nguyen Xuan Nhiem,Nguyen Xuan Cuong,BUIHUU TAI,TRANHONG QUANG,Hoang Le Tuan Anh,Pham Hai Yen,Ninh Khac Ban,김승현,Mingjie Xin,차지윤,이영미 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12
Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Ztrienoylglyceryl-O-β-D-galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O-β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.