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Proteome alterations towards understanding molecular mechanism upon copper stress in Sorghum
Swapan Kumar Roy,Soo Jeong Kwon,Won-Ju Lee,Jong-Ho Yang,Sang-Woo Kim,Tae-Wook Jung,Jung-In Kim,Tae-Seok Ko,Sun-Hee Woo 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Copper (Cu) is an essential micronutrient required for growth and development of plants. But, at a high concentration in soil, copper acts as a major toxic element to plant cells due to its potential inhibitory effects against many physiological and biochemical processes. In this study, the morphological and physiological changes were observed in the leaf of sorghum plants treated with different concentrations (0, 100, and 150 μM) of Copper (Cu). The results linked to morphological changes that plants treated with Cu suffered reduction in growth and morphological changes. In the ion concentration investigation, the concentrations of Cu2+ increased, the concentration of others interacting ions (Zn2+, Ca2+, Mn2+, Fe2+) were changed dramatically. For proteome analysis, 2-D combined with MALDI-TOF-TOF mass spectrometry was performed. Two dimensional gels stained with silver staining, a total of 422 differential expressed proteins (≥ 2-fold) were identified using Progenesis SameSpot software. A total of 24 spots from Cu-induced sorghum leaf and 21 spots from Cu-induced sorghum root were analyzed by mass spectrometry. Out of 24 protein spots from Cu-stressed leaf, of which 16 protein spots were up-regulated and 8 protein spots were down-regulated whereas out of 21 protein spots, a total of 9 protein spots were up-regulated and 12 spots were down-regulated from Cu-stressed root. Taken together, these studies revealed the effects of heavy metal, Cu on the growth and physiological characteristics in sorghum seedlings and proteome investigation, hoping to provide references on the mechanism of heavy metal damaging plants.
Swapan Kumar Bagui,Ambarish Ghosh 대한토목학회 2012 KSCE JOURNAL OF CIVIL ENGINEERING Vol.16 No.7
Drivers of vehicles are facing problem to drive due to the glare of head light of the opposite vehicle. This reduces the speed of vehicle during night driving for the case of not providing anti glare screen barrier on the median of the divided carriageway. This speed can be increased by providing anti glare screen barrier on the median of divided carriageway. Different values of Vehicle Operating Cost (VOC) have been found for both the cases. The values of VOC savings 40% and 15% are recommended as toll tariff for commercial and passenger/bus vehicle respectively. Financial analysis has been carried out using toll rate calculated from VOC savings. Toll revenue has been determined considering effects of local traffic, leakage of traffic and toll exempted traffic. These toll rates are used to carry out financial analysis to determine optimal debt capacity ratio. This paper presents the development of a model to determine the optimal debt equity ratio based on equal and variable repayment schedule as well as proposing equal and variable depreciation and identify best model for using equity holder. The model is the combination of a financial model and a linear programming model that incorporates an objective of maximizing the return of the project from the equity holder’s point of view and identify best method of repayment schedule from promoter point of view. Equal installment schedule with Written Down Value i. e.,variable Depreciation Method is the best method which maximizes return on equity. To show the versatility of the model, a real case study has also been presented herein.
Mass Chain Evaluation for A = 95
Swapan Kr. Basu,Gopal Mukherjee,A. A. Sonzogni 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23
A full evaluation of the mass chain A = 95 has been done in the ENSDF format taking into account all the available data until June 2009. Excited states populated by in-beam nuclear reactions and by radioactive decay have been considered. The "evp" editor, developed at the NNDC, has been used for the evaluation. This mass chain was last evaluated in 1993. Many new and improved data were reported since then. A total of 13 nuclei have been evaluated.
Hard tissue regeneration using bone substitutes: an update on innovations in materials
Swapan Kumar Sarkar,이병택 대한내과학회 2015 The Korean Journal of Internal Medicine Vol.30 No.3
Bone is a unique organ composed of mineralized hard tissue, unlike any other body part. The unique manner in which bone can constantly undergo self-remodeling has created interesting clinical approaches to the healing of damaged bone. Healing of large bone defects is achieved using implant materials that gradually integrate with the body after healing is completed. Such strategies require a multidisciplinary approach by material scientists, biological scientists, and clinicians. Development of materials for bone healing and exploration of the interactions thereof with the body are active research areas. In this review, we explore ongoing developments in the creation of materials for regenerating hard tissues.
High-throughput in itro culture system targeting genetic transformation in sugarcane
Swapan K. Tripathy,Dinesh Manohar Ithape 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.4
Conventional recombination breeding suffers setbacks for genetic improvement of sugarcane owing to its narrow gene pool, large complex genome, rare flowering, low fertility, long breeding cycle, and complex environmental interactions. However, production of transgenic plants can be a better alternative to improve quality traits and resistance to biotic and abiotic stresses. The authors report a ready in hand efficient in vitro culture protocol for genetic transformation in a popular sugarcane cv. Sabita (CoOR 03151) of Odisha. 2, 4-D at 3.0 mg/l resulted in highest callus induction frequency (87.8%) with white, friable, and nodular embryogenic calli suitable for plantlet regeneration. 2 mg/l BAP resulted in moderately higher number of shoots/responsive callus and higher percentage of plant survival. However, addition of TDZ (0.05 m/l) with 2 mg/l BAP in R medium (modifi ed MS) may be considered optimum for regeneration of profuse healthy plantlets. A combination of PBZ (0.05 mg/l) with BAP (2 mg/l) and TDZ (0.05 mg/l) revealed profuse multiple shoots (25 microshoots/responsive callus) and higher percentage of survival during follow-up plant establishment than above hormonal supplementation. Among various hormone recipes in MS medium with 3.0 mg/l NAA resulted in excellent rhizogenesis response (88.0%) with more or less normal rooting within 2 weeks. The above efficient and highly reproducible in vitro culture system can be amenable for genetic transformation in this crop.
Fabrication and Characterization of Porous TCP coated Al2O3 Scaffold by Polymeric Sponge Method
Swapan Kumar Sarkar,김영희,김민성,민영기,양훈모,송호연,이병택 한국세라믹학회 2008 한국세라믹학회지 Vol.45 No.10
A porous Al2O3 scaffold coated with tricalcium phosphate (TCP) was fabricated by replica method using polyurethane (PU) foam as a fugitive material. Successive coatings of Al2O3 and hydroxyapatite (HAp) were applied via dip coating onto polyurethane foam, which has a slender and well interconnected network. A porous structure was obtained after sequentially burning out the foam and then sintering at 1500oC. The HAp phase was changed to TCP phase at high temperature. The scaffold showed excellent interconnected porosity with pore sizes ranging from 300~700 μm in diameter. The inherent well interconnected structural feature of PU foam remained intact in the fabricated porous scaffold, where the PU foam material was entirely replaced by Al2O3 and TCP through a consecutive layering process. Thickness of the Al2O3 base and the TCP coating was about 7~10 μm each. The TCP coating was homogeneously dispersed on the surface of the Al2O3 scaffold.