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( Suwan Kim ),( Min Sik Jeong ),( Sang Hun Jung ),( Yong Hyun Lee ),( Jae Kweon Park ) 한국키틴키토산학회 2021 한국키틴키토산학회지 Vol.26 No.3
The complex of chitosan and hyaluronic acid was treated with enzyme to obtain chitosan oligosaccharide designated to COS hereafter. The degree of decomposition was confirmed by reducing sugar quantitative method, and the molecular weight size was confirmed by Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) mass spectrometry analysis. Based on the fact that the substrate specificity of the enzyme is highly dependent on temperature, substrate concentration, and pH, etc, the COS obtained by treating a mixture of chitosan and hyaluronic acid, which physically mixes well, under the non-optimal reaction conditions of the chitosanase. Accordingly, it exhibited very significant antibacterial activity against Meticillin Resistant Stapylococcs aureus (MRSA). As a method for controlling molecular weight of COS, it was intended to propose a new enzyme usage as a study to identify the characteristics of the hydrolysates according to the reaction delay and enzyme activity through complex formation.
SUWAN N. JAYASINGHE,SUMATHY ARUMUGANATHAR 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2007 NANO Vol.2 No.4
Fibers are structurally interesting components most useful in a range of applications spanning the physical and life science areas of research. These membrane (scaffold) forming fibers have been explored in applications ranging from microfilteration to advanced biological investigations in tissue engineering to controlled and targeted drug delivery. One such robust fiber generation approach investigated for over a century, which has recently been exploited, is the well-established threading process referred to as electrospinning. In this technique, single- or multi-phase media are charged within a conducting needle and later exposed to an electric field which promotes the formation of a continuous micro- to nanosized fiber(s) which over a period of collection time has been reported for forming scaffolds and membranes. This process has been explored for a wide range of polymer composite-based materials and the technique has now reached the point where it has moved into industrial production. We report here as a first example a comparable fiber to membrane fabrication approach completely driven by the coupling of a coaxial needle system with a pressure. We refer to this novel methodology as pressure-assisted spinning (PAS) where the hazardous element of high voltage (as in the case of electrospinning) is nonexistent. Hence, our discovery introduces both a directly competing fiber, scaffold to membrane fabrication approach, which is versatile and has no associated hazards as those in electrospinning. Furthermore as our technique is nonelectric field driven, the media spun into fibers could have a high electrical conductivity, which in this case has no effect on the stability in processing near-uniform fibers/scaffolds to membranes. The fabricated fibers and membranes generated by means of this approach could directly be used for a plethora of applications spanning the engineering and biological areas of research.
ECO 라우팅 단계에서의 셀 교환을 통한 회로 타이밍 향상
김수완(Suwan Kim),김태환(Taewhan Kim) 대한전자공학회 2020 대한전자공학회 학술대회 Vol.2020 No.11
Circuit timing is a critical factor regarding the performance of a chip. As the feature size of semiconductor decreases, the interconnecting wires become thinner. Since thin wires have high resistance, increasing the portion and variability of wire delay, a much careful attention should be paid on considering the effect of wire delay on the critical path of circuit. In this respect, this work proposes a way of shortening the critical path delay by locally swapping cells in the ECO (engineering change order) routing stage. Through experiments with benchmark circuits, it is shown that our method is able to reduce the worst negative slack by 0.95% on average.