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Resistance of Cryptosporidium parvum oocysts following commercial bleach treatment
Surl, Chan-gu,Jung, Bae-Dong,Park, Bae-Keun,Kim, Hyeon-cheol The Korean Society of Veterinary Science 2011 大韓獸醫學會誌 Vol.51 No.2
We investigate the resistance of Cryptosporidium (C.) parvum oocysts to commercial bleach treatment. The viability and infectivity of C. parvum oocysts suspended in 100, 50, 25, 12.5, 6.3 or 3.2% aqueous commercial bleach for 10, 30, 60, 120 or 180 min at room temperature were assessed by nucleic acid Syto-9 staining, histologic examination of ileum and infectivity to immunosuppressed neonatal C57BL/6N mice. Although the viability was decreased compared with normal oocysts, all oocysts in contact with serially diluted commercial bleach for 180 min were alive by nucleic acid dye Syto-9 staining. And, microscopic examination of ileum sections revealed developmental stages of C. parvum in all mice. The oocyst shedding patterns between mice infected with oocysts contacted with commercial bleach and normal control mice were not significantly different each other. Although commercial bleach is widely used as a bacterial and viral disinfectant, the present findings indicate that it is not an effective disinfectant for C. parvum oocysts under practical conditions. Authors conclude that, therefore, it is undesirable to recommend commercial bleach as a disinfectant for C. parvum oocysts.
이희자,배정설,조광연,장순욱 한국조리과학회 1990 한국식품조리과학회지 Vol.6 No.4
Lipids of Job's tears (Yullmoo) were extracted by the mixture of chloroform: methanol (2:1, v/v) and from these lipids, triglycerides were separated by column chromatography (Sephadex G-25 column) and then confirmed by thin layer chromatography (TLC) and reconfirmed by gas chromatography (GC). The triglycerides were fractionated into 6 groups on the basis of their partition numbers (PN) by reverse phase high performance liquid chromatography (HPLC) on a column Shimpack CLC-ODS using tetrahydrofuran-acetonitril (25:75) mixture as a solvent. Each of these collected fractions gave one to two peaks in the GC chromatograms according to the acyl carbon number (CN) of the triglyceride and fatty acid composition of the triglycer ides were also analyzed by GC. From the results, the major triglycerides of Job's tears lipids were estimated to be C18:1 C18:2 C18:2 (OLL, 38.2%), C16:0 C18:2 C18:3 (PLLn, 15.7%), C18:1 C18:1 C18:2 (OOL, 12.6%).
안용근,배정설 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.2
한국산 청주 두 종류, 일본산 청주 다섯 종류의 당을 HPLC 및 TLC로 비교 분석하였다. 총당은 한국산, 일본산 모두 평균 4.69%를 나타냈고, 한국산은 4.21% 및 4.93%를 나타냈다. 글루코오스는 평균 3.23%를 나타냈고, 한국산은 3.29%, 3.68%를 나타냈다. 말토오스에서 말토옥타오스까지의 말토올리고당은 평균 1.46%를 나타냈다. 청주의 당을 사람타액 α-amylase와 Aspergillus awamori α-glucosidase로 가수분해한 결과 모두 글루코오스로 가수분해되어 비피두스균 활성화 효과는 적은 것으로 나타났다. Sugars in 2 brands of Korean Sake and 5 brands of Japanese Sake were analyzed by HPLC and TLC. Sake was found to contain average 4.6% of total sugar, 2.23% of glucose and 1.46% of maltooligosaccharides from maltose to maltodecaose. Korean two brands were found to 4.21% of total sugar, 3.68% and 3.29% of glucose and 1.25% and 0.92% of maltooligosaccharides from maltose to maltoedcaose, respectively. Maltooligosaccharides in Sake were digested with α-amylase plus Aspergillus awamori α-glucosidase and produced glucose. The results suggested that sugars in Sake is not effective as a growth factor or Bifidobacterium.
이정원 ( Jeoung Won Lee ),설찬구 ( Chan Gu Surl ),김종승 ( Chong Sung Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3
In order to monitor the parasites 417 fecal samples were taken from ogol chicken in Chonbuk area, The isolation and identification of the parasites were determined by the fecal examination using the floatation and microscopical examination, respectively. The detection rate of the eggs and unsporulated oocysts from the feces was 47.7%(199 heads), in the concerns of complicated infection with single, double and triple were 36.6%(153 heads), 9.5%(40 heads) and 1.4%(6 heads), respectively. The parasites isolated were identified as eimeria spp from 115 heads, dermanyssus spp from 103 heads, ascaridia galli from 26 heads, pterolichus obtusus from 13 heads, capillana spp from 6 heads and heterakiss gallirarwn from 1 head, in order.