Lack of Relation of AKAP12 with p53 and Bcl-2 in Colorectal Carcinoma

Suren, Dinc,Yildirim, Mustafa,Alikanoglu, Arsenal Sezgin,Kaya, Vildan,Yildiz, Mustafa,Dilli, Utku Donem,Sezer, Cem Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: AKAP12 inhibits oncogenic proliferation, invasion, chemotaxis and neovascularization. Bcl-2 and p53 are two important apoptotic markers that play roles in apoptotic processes. It has been found that AKAP12 blocks the cell cycle and induces apoptosis in fibrosarcoma cells. In our study we assessed the relationship of AKAP12 with apoptotic markers, Bcl-2 and p53. Materials and Methods: Our study included 45 cases that were histopathologically diagnosed with colorectal carcinoma from the tissue samples acquired by surgical resection. AKAP 12, Bcl-2, and p53 expression was examined by immunohistochemistry. Results: A total of 45 colorectal adenocarcinoma patients - 17 (37.8%) females and 28 (62.2%) males - were included in this study. AKAP12 expression was found to be negative in 8 patients (17.8%), and positive in 37 patients (82.2%). Bcl-2 was found positive in 6 patients (13.3%) and p53 in 29 patients (55.6%). AKAP12 expression had no significant relation with Bcl-2 and p53 expression (p:0.939, p:0.079, respectively). Conclusions: Although various studies have pointed to apoptotic activity of AKAP12, the literature is limited regarding relations with p53 or Bcl-2 expression. In the present study, we found no relation in colorectal carcinomas.

Simultaneous extraction and stripping of lead ions via a hollow fiber supported liquid membrane: Experiment and modeling

Sira Suren,Ura Pancharoen,Soorathep Kheawhom 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4

An investigation on the separation of Pb(II) from synthetic wastewater via a hollow fiber supported liquid membrane (HFSLM) and its model is presented. Maximum percentages of Pb(II) extraction and stripping achieved were 97% and 30% using 0.03 M D2EHPA as the extractant, 0.9 M HCl as the stripping solution. The extraction and stripping of Pb(II) were second-order reaction with reaction rate constants of 0.03 and 0.19 L/mg s, respectively. A mathematical model is developed and shows that the model results fit in well with the experimental results. Average percent deviation was 3% for predictions in both feed and stripping sides.

Discriminatory experiences of international students in South Korea

Dembereldorj Zoljargal,Suren Chimed 한국교육정치학회 2014 敎育政治學硏究 Vol.21 No.2

The study explains discriminatory experiences of international students in one of the campuses in South Korea through the lens of neo-racism. Neo-racism is a theory of segregation that postulates discrimination is based on individual’s national identity, culture, and country of origin. For deeper understanding discriminatory perceptions of international students, the study applied a qualitative study with semi-structured interviews with purposive sampling at one aggressively internationalizing university. The study found implicit and explicit forms of discriminatory experiences, discriminatory perceptions of exclusion, different treatments based on nationality, lack of cultural knowledge, stereotype, and English language ability. Drawing from the findings, the study concludes that discriminatory experiences of international students are more status-based rather than country of origin based in a context of South Korea. It suggests that discrimination as segregation exists, and therefore, it is desirable to consider this issue into relevant policies and practices.

Tumour Markers in Peritoneal Washing Fluid - Contribution to Cytology

Yildirim, Mustafa,Suren, Dinc,Yildiz, Mustafa,Alikanoglu, Arsenal Sezgin,Kaya, Vildan,Doluoglu, Suleyman Gunhan,Aydin, Ozgur,Yilmaz, Necat,Sezer, Cem,Karaca, Mehmet Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Background: Peritoneal washing cytology (PWC) that shows the microscopic intra-peritoneal spread of gynaecologic cancers is not used in staging but is known as prognostic factor and effective in planning the intensity of the therapy. False negative or false positive results clearly affect the ability to make the best decision for therapy. In this study we assessed levels of tumour markers, carcinoembryonic antigen (CEA), cancer antigen 125 (CA-125) and carbohydrate antigen (CA19-9), in peritoneal washing fluid to establish any possible contribution to the peritoneal washing cytology in patients operated for gynaecologic cancer. Materials and Methods: Preoperative tumour markers were studied in serum of blood samples obtained from the patients for preoperative evaluation of a gynaecologic operation. In the same group peritoneal tumour markers were studied in the washing fluid obtained for intraoperative cytological evaluation. Results: This study included a total of 94 patients, 62 with malignant and 32 with benign histopathology. The sensitivity of the cytological examination was found to be 21% with a specificity of 100%. When evaluated with CEA the sensitivity of the cytological examination has increased to 37%. Conclusions: In addition to examination of PWC, the level of CEA, a tumour marker, in peritoneal washing fluid can make a diagnostic contribution. Determining the level of CEA in peritoneal washing fluid will be useful in the management of gynaecologic cancers.

Multiparameter fluorescence image spectroscopy to study molecular interactions

Weidtkamp-Peters, Stefanie,Felekyan, Suren,Bleckmann, Andrea,Simon, Rudiger,Becker, Wolfgang,Kuhnemuth, Ralf,Seidel, Claus A.M. Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.4

Multiparameter Fluorescence Image Spectroscopy (MFIS) is used to monitor simultaneously a variety of fluorescence parameters in confocal fluorescence microscopy. As the photons are registered one by one, MFIS allows for fully parallel recording of Fluorescence Correlation/Cross Correlation Spectroscopy (FCS/FCCS), fluorescence lifetime and pixel/image information over time periods of hours with picosecond accuracy. The analysis of the pixel fluorescence information in higher-dimensional histograms maximizes the selectivity of fluorescence microscopic methods. Moreover it facilitates a statistically-relevant data analysis of the pixel information which makes an efficient detection of heterogeneities possible. The reliability of MFIS has been demonstrated for molecular interaction studies in different complex environments: (I) detecting the heterogeneity of diffusion properties of the dye Rhodamine 110 in a sepharose bead, (II) F$\ddot{o}$rster Resonance Energy Transfer (FRET) studies in mammalian HEK293 cells, and (III) FRET study of the homodimerisation of the transcription factor BIM1 in plant cells. The multidimensional analysis of correlated changes of several parameters measured by FRET, FCS, fluorescence lifetime and anisotropy increases the robustness of the analysis significantly. The economic use of photon information allows one to keep the expression levels of fluorescent protein-fusion proteins as low as possible (down to the single-molecule level).

Biocontrol of Listeria monocytogenes ATCC 7644 on fresh-cut tomato (Lycopersicon esculentum) using nisin combined with organic acids

Adebola Olubukola Oladunjoye,Suren Singh,Oluwatosin Ademola Ijabadeniyi 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

The biocontrol of Listeria monocytogenes on fresh-cut tomato using nisin and organic acids was investigated. Fresh-cut samples inoculated with 108 CFU/mL of L. monocytogenes, treated with nisin (5,000 IU/mL), a combination of nisin and organic acids (acetic and citric acids at 3 and 5% each), with chlorine at 200 ppm as a control, and stored for six days at 4, 10, and 25oC were used to evaluate certain physicochemical qualities (pH, titratable acidity, soluble solid content, vitamin C content, and color). Nisin treatment significantly (p<0.05) reduced bacterial population by 1.91-3.07 log CFU/mL. Nisin-citric acid combination provided 2.65-3.29 log CFU/mL reduction, while nisin-acetic acid combination provided 2.93-4.15 log CFU/mL reduction. The control treatment provided <1-2 fold log reductions. Slight variations in physicochemical properties of fresh-cut tomato were observed. Nisin and organic acids can be used to improve the microbial safety of fresh-cut tomato.

Mechanisms of mercury ions separation by non-toxic organic liquid membrane via DFT, thermodynamics, kinetics and mass transfer model

Natthapol Traiwongsa,Sira Suren,Ura Pancharoen,Kasidit Nootong,Kreangkrai Maneeintr,Wikorn Punyain,Anchaleeporn W. Lothongkum 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

This work focuses on the separation of mercury ions from synthetic produced wastewater via hollowfiber supported liquid membrane (HFSLM). Standard sunflower oil is used as a non-toxic extractantand solvent for mercury ions separation from wastewater. Under optimal conditions: 20 ppm NaCl inwastewater, 0.1 M thiourea in 0.5 M HCl as stripping solution, flow rates of feed and stripping solutionsof 100 mL/min, and temperature 323 K, percentages of extraction and stripping reach 98% and 81%,respectively. The mechanisms of extraction and stripping reactions were investigated via the densityfunctional theory (DFT). For the extraction, a linoleic acid extracted HgCl2 and formed halogen bond. For the stripping, two protonated thiourea stripped HgCl2 and formed covalent and halogen bonds. According to the thermodynamics study, extraction reaction is found to be endothermic (DH0Ex = 1,001. 00 J/mol), irreversible (DS0Ex = 3.45 J/mol K), and spontaneous, (DG0Ex@323 K = 112.65 J/mol). Finally, themathematical model including conservation, convection, diffusion, reaction, and accumulation wasdeveloped. The differences of mercury ions concentration in feed and stripping phases from the experimentsand the predicted data from the mathematical model were 5% and 2%, respectively indicating thatthe model is acceptable.

Biocontrol of Listeria monocytogenes ATCC 7644 on fresh-cut tomato (Lycopersicon esculentum) using nisin combined with organic acids

Oladunjoye, Adebola Olubukola,Singh, Suren,Ijabadeniyi, Oluwatosin Ademola 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

The biocontrol of Listeria monocytogenes on fresh-cut tomato using nisin and organic acids was investigated. Fresh-cut samples inoculated with $10^8CFU/mL$ of L. monocytogenes, treated with nisin (5,000 IU/mL), a combination of nisin and organic acids (acetic and citric acids at 3 and 5% each), with chlorine at 200 ppm as a control, and stored for six days at 4, 10, and $25^{\circ}C$ were used to evaluate certain physicochemical qualities (pH, titratable acidity, soluble solid content, vitamin C content, and color). Nisin treatment significantly (p<0.05) reduced bacterial population by 1.91-3.07 log CFU/mL. Nisin-citric acid combination provided 2.65-3.29 log CFU/mL reduction, while nisin-acetic acid combination provided 2.93-4.15 log CFU/mL reduction. The control treatment provided <1-2 fold log reductions. Slight variations in physicochemical properties of fresh-cut tomato were observed. Nisin and organic acids can be used to improve the microbial safety of fresh-cut tomato.

Experimental and DFT investigations on the supramolecular mechanism of Ni(II) extraction via D2EHPA blended 1-octanol extractant: Application of vegetable oils as diluents

Watcharapong Ampronpong,Sira Suren,Vanee Mohdee,Kreangkrai Maneeintr,Sanong Ekgasit,Tatchanok Prapasawat,Wikorn Punyain,Ura Pancharoen 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.3

This work presents the separation of trace Ni(II) from aqueous solution via liquid-liquid extraction. The application of vegetable oils (rice bran, soybean and sunflower oil) as alternative green solvents was evaluated. Results demonstrate that D2EHPA dissolved in rice bran oil provides the highest extraction of Ni(II), whereas D2EHPA dissolved in kerosene reveals much lower performance. Furthermore, extraction of Ni(II) dramatically increases when the binary system of D2EHPA/1-octanol is applied, suggesting the occurrence of synergism having the synergistic coefficient=3.580. Under optimum conditions, extraction and stripping of Ni(II) achieved 99.70% and 88.00%, complying with the wastewater discharge limit in Thailand. Herein, a novel approach involving supramolecular mechanisms via density functional theory in a binary system of D2EHPA/1-octanol is first reported. Results show that intermolecular hydrogen bonds play an important role in supramolecular complexing. Fourier transform infrared spectrometer is analyzed to verify the functional groups in which the reactions occur. Significant numeric results are as follows: kextraction=0.134 s−1, kstripping=0.038 s−1, ΔH0=72.759 kJ/mol and ΔS0=0.268 kJ/mol·K.

Forensic and population genetic analyses of the GlobalFiler STR loci in the Mongolian population

최은지,박기원,이양한,남윤형,Ganbold Suren,Uyanga Ganbold,김지애,김소연,김혜민,김기철,김욱 한국유전학회 2017 Genes & Genomics Vol.39 No.4

We have analyzed 24 loci including autosomal and Y-chromosomal short tandem repeats (STRs), Y-indel, and sex-determining marker in a sample of 267 unrelated individuals from the Mongolian population using the GlobalFiler™ PCR Amplification Kit to provide an expanded and more reliable forensic database. Khalkh among 15 Mongolian minor-groups accounts for about 80% of the entire Mongolian population. A total of 267 different DNA profiles were found in this work. The highest gene diversity was observed in the SE33 (0.9376) locus, and the lowest value was found in the TPOX (0.6142) locus. Although individual power of discrimination estimates varied at the studied loci, combined probability of match from the 21 STR loci was estimated to be 1.139 × 10−24, which is highly informative. Based on the results of pairwise FST genetic distances and multi-dimensional scaling plot showed that Mongolians were clustered into Europeans and Asians, although Mongolia is geographically located in Northeastern Asia. Thus, the present survey of the Mongolian population may help establish a comprehensive reference database for forensic and population genetic analyses.