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H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향
정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4
Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.
Paola Marques de Mattos,Vula Papalexiou,Vinícius Augusto Tramontina,Sung Hyun Kim,Sônia Mara Luczyszyn,Patrícia Vida Cassi Bettega,Aline Cristina Batista Rodrigues Johann 대한치주과학회 2020 Journal of Periodontal & Implant Science Vol.50 No.1
Purpose: Subepithelial connective tissue grafts (SCTGs) are commonly performed for the treatment of gingival recession due to their high predictability. This study evaluated and histologically compared connective tissue grafts in terms of the presence of epithelial remnants and composition of the tissue types that were present (epithelium, lamina propria, and submucosa). Methods: Ten patients underwent epithelium removal using 2 different techniques: the use of a blade (group B) and through abrasion (group A). Twenty samples were collected and each tissue type was analyzed histologically in terms of its area, thickness, and proportion of the total area of the graft. Results: In 4 samples (40%) from group B (n=10) and 2 samples (20%) from group A (n=10), the presence of an epithelial remnant was observed, but the difference between the groups was not statistically significant ( P >0.05). Likewise, no statistically significant differences were observed between the groups regarding the area, mean thickness, or proportion of the total area for any of the tissue types ( P >0.05). Conclusions: Histologically, SCTGs did not show statistically significant differences in terms of their tissue composition depending on whether they were separated from the epithelial tissue by abrasion or by using a blade.
강혜숙,남은우,박성빈,최재임 고신대학교 영도발전연구소 2000 영도연구 Vol.2 No.-
The purpose of this study was to grasp the sex knowledge in middle and high school students in Youngdo-Gu, Busan, and to provide some basic data for the needs and the direction of sex education for the teenagers. The survey was conducted by using questionnaires in two middle schools and two high schools in Youngdo-Gu. The questionnaires were took back after record by the respondents. The survey was conducted at September 15∼17, 2000 and a total of 791 were completed. The respondents were 200 (25.3%) males and 199 (25.2%) females in middle schools and 196 (24.8%) males and 196 (24.8%) females in high schools. For the statistical analysis, χ2-test, t-test and one-way ANOVA were used by using SPSS/PC+ WIN 8.0 program. The conclusions based on the results obtained from this study are as follows: 1) An attitude of the sexual matters showed that boys, students and non-christians were more progressive than girls, parents and christians, respectively (p<0.01). 2) The sexual agonies of students were in order of having a date 36.2%, sexual drive 11.1%, sexual intercourse 8.8%, masturbation 8.6% and sexual abuse 6.7%. 3) The points for sexual knowledges appeared 40.6 points out of 100 points from boys and 42.3 points from girls in middle schools, and 57.3 points from boys and 66.1 points from girls in high schools. Therefore, the girls had much knowledges on sexuality than those of the boys. There were a significant differences of knowledge on sexuality between boys and girls (p<0.001). Over 60% of both boys and girls had not enough knowledges on sexually transmitted diseases (STDs). 4) Satisfaction of sex education was common or unsatisfied as over 90% of respondents. The reasons were a textbook-like general information (50%) and nothing practically to help (29%). 5) The students wanted sex education for the practice in future life such as male and female relationship with etiquette(23%); and pregnancy and delivery (17%) in middle school students, and contraceptive and abortion (34%) and preventive STDs (18%). There was not enough to understand whole adolescent in Youngdo area because the study subjects were only public middle and hight school students in Youngdo-Gu, Busan. Conclusively, it is necessary to educate to give right information of sexuality to the adolescents in Youngdo, and to have systematically sex education from the kindergartens to the high schools by long range sex educational program. Also, a proper program for integrated sex education is necessary to build healthful sexual consciousness and worth in cooperation with sex educators, universities and health centers.
Caffeine이 지구성 운동 수행능력에 미치는 영향과 억제성 아미노산에 의하여 활성화되는 이온통로의 조절작용
김은경,김영표,천병옥,이계영,김연정,임백빈,조영욱,김창주,김성수 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1
To investigate the effect and mechanism of caffeine on endurance exercise, two experiments were performed. First, to test caffeine effect on aerobic exercise, 200-300g Sprague-Dawley rats were used and three groups, control group, low caffeine injection group and high caffeine injection group, were divided. Blood smpling by heart puncture were done at rest, after 30 min treadmill exercise, and after maximal exercise. Blood glucose, free fatty acid concentration were detected and following results were obtained. Glucose concentration showed significant difference between groups(p=0.0305) and also significant changes were exhibited between time(p=0.0004). Free fatty acid concentration had no difference between groups. but had significance between times(p=0.00065). Exercise endurance performance time showed significant difference(p=0.02350 in high caffeine injection group compared to control group. In this experiments, endurance exercise capacity was increased by caffeine injection. Therefore, second experiment was performed to investigate the effect of caffeine on ion current induced inhibitory amino acid neurotransmitter. GABA and glycine. Single periaqueductal gray neuron was acutely dissociated and nystatin perforated patch clamp was performed under voltage clamping condition. Caffeine evoked outward current in PAG neuron dose dependent manner. 1mM of caffeine application had no response. but 3mM caffeine evoked about 32.5±8.539pA outward ion current and 10mM caffeine evoked about 215.46±19.4pA outward current. 10^-2mM GABA activated Cl ̄current and recorded by inward current. Caffeine inhibited GABA activated Cl ̄ current concentration dependent manner. 10^-2mM of caffeine had no effect on 1-^-2mM of GABA response. but 10^-1mM caffeine inhibited GABA activated Cl ̄ current about 5.74±2.13%, 1mM caffeine inhibited about 17.25±2.70%, 10mM caffeine inhibited GABA response about 45.31±7.71%. 10^-1mM of glycine activated Cl ̄ current and also recorded by inward current. Caffeine inhibited glycine activated Cl ̄ current concentration dependent manner. 10^-2mM caffeine decreased glycine activated Cl ̄ current about 4.61±1.650%, 10^-1mM caffeine decreased about 6.49±2.24%, 1mM caffeine decreased about 26.82±4.27%, and 10mM caffeine decreased glycine response about 94.47±1.39%. These results suggest that caffeine inhibite inhibitory amino acid, GABA and glycine, this response causes excitation of CNS and this seems to be the basic mechanism of increasing effect to aerobic exercise performance by caffeine.
Angiotensin 전환효소 유전자 다형성과 양극성 장애
김경나,김종우,정주호,이기철,정홍경,임성빈 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2
연구목적 : 감정조절에 Renin-Angiotensin System이 관여하는 것으로 보고되어 왔다. 그 근거로 우울증의 새로운 약물치료로 주목받고 있는 Substance P의 대사에 angiotensin 전환효소(angiotensin-converting enzyme, ACE)가 관여한다는 것과 고혈압 환자에서 angiotensin 전환효소 억제제(ACE inhibitor)를 사용했을 때 다행감이나 우울감을 초래한다는 것 등이 있다. 본 연구에서는 ACE의 유전자 다형성을 분석하여 양극성장애와 ACE와의 연관성을 알아보고자 하였다. 방 법 : 양극성장애로 진단된 환자군 82명과 대조군 135명을 대상으로 16번째 intron의 다형성 부위를 가진 시발체 쌍(primer pair)을 사용하며 중합효소 연쇄반응(Polymerase Chain Reaction, PCR)을 시행하여 490bp 산물(I allele)과 190bp 산물(D allele)을 관찰함으로써 ACE 유전자의 유전자형(genotype)의 발현율과 대립유전자(allele)의 빈도를 비교 분석하였다. 결 과 : 양극성장애 환자군과 대조군 사이에서 유전자형의 발현율과 대립유전자적 빈도는 유의한 차이가 없었다. 결 론 : 양극성장애 환자군과 ACE 유전자 다형성 사이에 유의한 관련성은 없었다. 이 결과는 ACE가 양극성 장애의 원인으로 주요한 역할을 한다는 것을 시사하지 못했다. Objectives : A possible participation of the Renin-Angiotensin Systern(RAS) in regulating of the mood has been suggested by reports as follows : the angiotensrn converting enzyme(AGE1 is involved jn the metabolism of the neuropeptide substance P impficated with novel strategies for the pharmacotherapy of depression and the use of ACE inhibitors in hypertensive patients has been associated with euphoric or depressive stales. The aim of the present study was to analyze the role of the ACE gene I/D polymorphism for bipolar disorder. Methods : We examined the frequency of a polymorphism characterized by the insertion or deletion of a 287-bp Alu repeat sequence in intron 16 of the angiotensin converting enzyme gene(located on chromosome 37q233 in groups of patients with bipolar disorder(n=82) compared to healthy control subjects[n=135). ACE genotype was determined by size-analysis of polymerase chain reaction products. Results : The ACE ID polymorphism did not show any difference in allelic frequencies and genotypic distributions between bipolar disorder patients and control subjects. Conclusions : No significant association was found with bipolar disorder and the polymorphism of ACE gene. This finding does not support that ACE I/D polymorphism is a significant risk factor for bipolar disorder.
원보 : 파스틱 정(R)(나테글리니드 90mg)에 대한 글루나테 정(R)의 생물학적 동등성
탁성권 ( Sung Kwon Tak ),이진성 ( Jin Sung Lee ),최상준 ( Sang Joon Choi ),서지형 ( Ji Hyung Seo ),이명재 ( Myung Jae Lee ),강종민 ( Jong Min Kang ),류주희 ( Ju Hee Ryu ),홍승재 ( Seung Jae Hong ),임성빈 ( Sung Vin Yim ),이경태 ( 한국약제학회 2009 Journal of Pharmaceutical Investigation Vol.39 No.2
단보 : 인체 혈장중 라베프라졸의 정량을 위한 LC-MS/MS 분석법 검증 및 단일 용량 투여에 의한 약물동태 연구
탁성권 ( Sung Kwon Tak ),서지형 ( Ji Hyung Seo ),류주희 ( Ju Hee Ryu ),최상준 ( Sang Joon Choi ),이명재 ( Myung Jae Lee ),강종민 ( Jong Min Kang ),이진성 ( Jin Sung Lee ),홍승재 ( Seung Jae Hong ),임성빈 ( Sung Vin Yim ),이경태 ( 한국약제학회 2009 Journal of Pharmaceutical Investigation Vol.39 No.1