Complete nucleotide sequence of the penicillin G acylase gene and the flanking regions, and its expression in Escherichia coli

오상진, 김영창, 박영우, 민소영, 김인숙, 강현삼 忠北大學校 遺傳工學硏究所 1988 遺傳工學硏究 Vol.2 No.1

본문참고

Korean English Teacher's Disempowerment in English-only Classes

Sung-Sam Hwang,Hyo-Sun Seo,Tae-Young Kim 한국사회언어학회 2010 사회언어학 Vol.18 No.1

Hwang, Sung-Sam, SeD, Hyo-Sun, Kim, Tae-Young. 2010. Korean English Teacher's Disempowerment in English-only Classes: A Case Study Focusing on Korea-specific Cultural Aspects. The Sociolinguistic journal of Korea 18(1). In this case study, we have focused on the disempowerment of a Korean English teacher (KET) in English-only classrooms. Six English as a foreign language (EFL) classrooms in a Korean high school were observed and analyzed by using interpretive discourse analysis. Out of the six classes, three representative classrooms were both video- and audio-taped. The results show that the English-only classes could weaken the KETs' power via three mechanisms: (a) calling the KETs' last name for choral greetings at the beginning of the class, (b) students' group solidarity and peer pressure between classmates, and (c) the students' excessively collaborative approach to peer assistance. It is argued that the exclusive English-only instruction disempowers the KETs' status and dismantles their vocational identity. In order to remedy the current situation, within a scaffolding supported by the eclectic use of first language where necessary for effective class management, KETs are empowered and overcome constraints imposed by the limited L2 oral competence of some non-native English teachers.

Post 공간형성이 치근단 폐쇄성에 미치는 영향에 관한 전기화학적 연구

임성삼 大韓齒科保存學會 1994 Restorative Dentistry & Endodontics Vol.19 No.2

전자선을 이용한 전신 피부 치료의 선량분포

김성환,박종삼,이준일 대구보건대학 1997 대구보건대학 論文集 Vol.17 No.-

Total Skin Electron Therapy(TSET) was used to treat superficially widespread skin legions such as mycosis fungoides and achronic progressive lymphoma. This paper described the dosimetry for the TSET at energy of about 6 MeV electron beams(Linear Accelerator, Siemens Mevatron KD) with dual field beams. The optimal conditions of the TSET were achieved when source skin distance was 500 cm, the angle between dual beams was 28^(。) and thickness of acryle filter was 0.8cm. At that time, the mean energy of electron beam was 3.8 MeV and the filed size of electron beam at the patients treatment plane was 180×100 ㎠. Within this rectangle, the uniformity of dose distribution was ±10% and the uniformity was ±5% except comer of the rectangle med. And the dose rate of the center was 11.7 cGy/min.

소음·진동에 따른 참전복(Haliotis dissus hannai)의 행동·생태학적 변화

최상덕,정형택,윤호섭,안치영,이삼노,이우범 國立 麗水大學校 환경문제硏究所 2001 環境硏究論文集 Vol.4 No.-

These days, the underwater excavation and the piling work have been commonly used to reclaim land and to build a breakwater. As the piling work generates high underwater sound pressure in all direction, various kinds of marine organisms are largely affected even if they live a long distance from the piling work. Therefore, this paper describes the relationship between the behavior of abalone(Haliotis discus hannai) of 50cm long and the environmental noise level with vibration due to the piling work. We have separated the research into two parts. The one is continuance impact, the other is intermittent impact. The following are the results obtained from the present investigation: Comparing 0.5cm/sec vibration with 50dB noise and 0.9cm/sec vibration with 60dB, the previous one show a faster reaction speed than the later one. Futhermore the later one showed that pulling up on their shell, in order to avoid the epidemic, moving around, small organisms are included to hide under big organisms, alerting posture. However we couldn't find a consistent standard in our research.

폐쇄성 수면무호흡 환자의 상기도 형태의 특징과 압력강하에 관한 3차원 전산유체역학해석

모성서,안형택,이정선,정유삼,문윤식,배응권,성상진 대한치과교정학회 2010 대한치과교정학회지 Vol.40 No.2

폐쇄성 수면무호흡(obstructive sleep apnea, OSA)은 수면 중에 반복적으로 상기도의 완전폐쇄나 부분폐쇄가 일어나는 질환으로서 흡기된 공기는 반드시 상기도라는 연조직 관(tube) 구조를 통과해야 하므로 상기도의 폐쇄경향은 관의 형태 및 관을 통과하는 공기의 유체역학적 특성에 따라서도 큰 영향을 받을 수 있다. 본 연구에서는 OSA 환자 3인의 치료 전 상기도 CT 이미지를 이용하여 개별화된 3차원 유한요소모델 A, B, C를 제작하고, 비공 당 170, 200, 230 ml/s의 흡기유량에 대하여 3차원 전산유체역학 해석을 시행하였다. 상기도의 유속, 음압 그리고 압력강하를 측정한 결과 관찰된 3개의 모델에서 모두 단면적이 가장 작은 부위에서 유속이 증가하였고, 음압이 크게 나타났다. 기도의 형태는 구개인두와 구인두 부위에서 좁아지는 형태를 가지며, 최소 단면적 영역과 하인두 단면적의 차이가 클수록 유속과 음압의 변화가 크게 나타났다. 비강 부위의 최고 압력과 최소 단면적 영역의 최저 압력의 차이를 의미하는 압력강하는 상기도 저항을 종합적으로 판단할 수 있는 유용한 지표이며, 유량에 따라 증가하였다. Objective: Obstructive sleep apnea (OSA) is a common disorder which is characterized by a recurrence of entire or partial collapse of the pharyngeal airway during sleep. A given tidal volume must traverse the soft tissue tube structure of the upper airway, so the tendency for airway obstruction is influenced by the geometries of the duct and characteristics of the airflow in respect to fluid dynamics. Methods: Individualized 3D FEA models were reconstructed from pretreatment computerized tomogram images of three patients with obstructive sleep apnea. 3D computational fluid dynamics analysis was used to observe the effect of airway geometry on the flow velocity, negative pressure and pressure drop in the upper airway at an inspiration flow rate of 170, 200, and 230 ml/s per nostril. Results: In all 3 models, large airflow velocity and negative pressure were observed around the section of minimum area (SMA), the region which narrows around the velopharynx and oropharynx. The bigger the Out-A (outlet area)/ SMA-A (SMA area) ratio, the greater was the change in airflow velocity and negative pressure. Conclusions: Pressure drop meaning the difference between highest pressure at nostril and lowest pressure at SMA, is a good indicator for upper airway resistance which increased more as the airflow volume was increased.

림프절 종창의 임상적 관찰

김삼용,김현수,김종숙,박상준,최지영,윤환중,조덕연 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Objectives : Clinical management of patient with lymphadenopathy requires judicious judgement of clinicians. Careful evaluation of lymph node enlargement by various clinical parameters will help to disclose the etiology of lymph node enlargement. This study was conducted to identify the clinical manifestations and etiology of lymphadenopathy in korean patients. Methods : A total 192 patients with lymph node enlargement were studied retrospectively. All patients who underwent excisional lymph node biopsy during the period from January 1992 to July 1994 were included. The lymph node biopsy and clinical records were reviewed and patient characteristics were analyzed by various clinical parameters. Results : A female predominance was noted in lymphadenopathy of tuberculosis (58.5%), Kikuchi's syndrome (70%). In lymphadenopathy of nonspecific, tuberculosis, kikuchi syndrome, per centage of patients younger than 40 years of age were 84.5%, 75.6%, 100%, respectively. In lymphadenopathy of tuberculosis and metastatic cancer, the duration of lymphadenopathy below 30days were 51.2%, 73.9%, respectively. The two most common etiology of cervical lymphadenopathy were nonspecific lymphadenitis (47.7%) and tuberculous (26.8%). The two most frequent etiology of supraclavicular lymphadenopathy were nonspecific lymphadenitis (46.3%) and metastatic lymphadenopathy (26.8%) and the most common etiology of axillary lymphadenopathy was nonspecific lymphadenopathy (34.5%). The most common frequent of lymph node enlargement with size below 1㎠ was nonspecific lymphadenitis, while above 2.25㎠ it was metastatic lymphadenopathy. Conclusion : Careful evaluation regarding patient age, sex, size of lymph node, consistency, location of lymph node, presence or absence of systemic symptoms should be pursued before performing a biopsy and management.

신경교세포 및 RAW 264.7 세포에서 Protein kinase의 활성에 의한 유도성 Nitric oxide synthase의 발현

박상철,노삼길,배소현,박지선,이충재,허강민,석정호,이재흔 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

NO(nitric oxide) plays an important role as neurotransmitter or cytokine, and pathologic factor for some diseases by the large amount production with iNOS(inducible NO synthase) expression in macrophages or glial cells. The expression of iNOS is regulated by various cytokines, protein kinases and transcription factors. In this experiment, to investigate the roles of progein kinase and NF-kB for iNOS expression, the effects of PMA(phorbol 12-myristate 13-acetate), cAMP, and various protein kinase inhibitors on LPS(lipopolysaccharide)-induced iNOS mRNAN expression and nuclear NF-kB binding complex were examined in C6 glial cells and RAW 264.7 cells. In C6 glial cells, iNOS mRNA expression by LPS was induced from 1 hour and peak at 3 hour after treatment. In RAW 264.7 cells, the mRNA was observed from 3 hour and peak at 6 hour. PMA enhanced markedly LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but did not much influence on LPS-induced iNOS mRNA expression in RAW 264.7 cells, in spite of increased LPS-induced NF-kB binding complex at 30 min. cAMP(dibutyryl cAMP) did not much influence on LPS-induced iNOS mRNA expression, by increased LPS-induced NF-kB binding complex in C6 glial cells. However, in RAW 264.7 cells, cAMP increased slightly LPS-induced iNOS mRNA expression without change of NF-kB binding complex. Staurosporine did not influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased LPS-induced iNOS mRNA expression and NF-kB binding complex. Ro-31-8220 did not much influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased significantly LPS-induced iNOS mRNA expression in spite of increased LPS-induced NF-kB binding complex for 3hours. G 6976 did not much influence on LPS-induced iNOS mRNA expression with decreased NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased iNOS mRNA expression without influence on LPS-induced NF-kB binding complex. Genistein did not influence on LPS-induced iNOS mRNA expression and NF-kB binding complex in C6 glial cells, but in RAW 264.7 cells, decreased LPS-induced iNOS mRNA expression inspite of increased NF-kB binding complex. These results suggest that LPS-induced regulation of iNOS expression or NF-kB activity in C6 glial cells, might be different from RAW 264.7 cells through various protein kinases or other factors.

Calcium Hydroxide 처리가 양앵두 果實의 品質에 미치는 영향

정삼택,김유신,전상항,김익홍 경북대학교 1990 새마을 硏究論叢 Vol.11 No.-

양앵두 佐藤錦 및 Napoleon品種을 供試하여 Ca(OH)_2 處理濃度 및 處理回數에 따른 果實生長, 果實品質, 칼슘축적 및 裂果에 미치는 影響을 調査하였던바 다음과 같은 結果를 얻었다. 1. 양앵두 果實生長은 佐藤錦품종에서 收穫1週前 Ca(OH)_2 처리농도간에는 차이가 없었으나, Napoleon품종에서 Ca(OH)_2 처리회수가 증가 할수록 抑制되었다. 2. 可溶性 固形物과 Anthocyanin함량은 Ca(OH)_2 처리농도 및 처리회수를 증가 할수록 감소하였으나, 유기산 함량은 증가하였다. 3. 果實內 칼슘축적은 Ca(OH)_2 처리농도 및 처리회수가 증가 할수록 증가하였고, 佐藤錦 품종에서 과실내 칼슘함량은 과피, 종자, 과육, 핵의 순이였고, Napoleon품종에서는 종자, 과피, 과육, 핵의 순이였다. 4. 裂果방지를 위한 Ca(OH)_2 처리는 7.2g/l를 수확 3주전부터 1週間隔으로 3회 살포하는 것이 열과를 감소시키는데 효과적이였다. The study was conducted to investigate the influences of several concentrations of Ca(OH)_2 and application time on fruit growth, quality, contents of Ca in fruits and cracking of 'sa Tou Nisiki' and 'Napoleon' sweet cherry. The results obtained are summarized as follows; 1. In Ca(OH)_2 treatments, the fruit growth of 'Sa Tou Nisiki' and 'Napoleon' sweet cherry was not influenced by its concentrations but by application times of Ca(OH)_2. 2. Contents of soluble solids and anthocyanin according to concentreation and application of Ca(OH)_2 were decreased, while titratable acidity was increased respectively. 3. Contents of Ca in the fruit according to concentration and application of Ca(OH)_2 were increased and Ca contents in both fruits differ from fruit portions. 4. Preharvest application of Ca(OH)_2 by 0.72% and three times applications reduced cracking of 'Sa Tou Nisiki' and 'Napoleon' sweet cherry. From these results, twice applications of Ca(OH)_2 to the sweet cherry are recommended for the best fruit quality, the authors believed.

IN VITRO STUDY ON APICAL SEAL AND SEM MORPHOLOGY IN CANALS FILLED WITH RESIN-BASED ROOT CANAL SEALER AND SELF-ETCHING PRIMER

강삼희,박동성,유현미,오태석 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.4

Gutta-percha와 근관sealer를 사용한 근관 충전은 가장 많이 이용되고 성공률이 높은 방법이지만, 현재 사용되는 모든 근관sealer는 미세누출을 나타낸다. 따라서 더 효과적인 근관 폐쇄를 이루기 위한 재료 및 방법들이 연구되어 왔다. 이중 상아질 접착제는 근관 충전재와 함께 사용되어 미세누출을 감소시키지만, 근관 내에 사용하기에 술식이 복잡하고 기술이 요구된다. 본 연구의 목적은 gutta-percha와 레진계 근관 sealer로 충전한 근관에서 self-etching primer를 미리 도포한 경우와 도포하지 않은 경우의 치근단 미세누출을 비교하고, self-etching primer를 도포함에 있어 근관 내에 적용하는 방법에 따른 미세누출을 비교하는 것이다. 또한 근관sealer와 상아질 계면을 주사전자현미경으로 관찰하여, 이 결과를 미세 누출과 관련시켜보고자 하였다. 36개의 발거된 사람의 단근치에서 치관부를 절단, 제거하고 ProFile로 근관 형성한 후, 무작위로 선택하여 4개의 군으로 분류하였다. 1군에서는 주사기와 30게이지 주사 바늘로 self-etching primer를 근관 내에 적용하였고, 2군에서는 self-etching primer를 paper point에 적셔 근관 내에 적용하였다. 3군에서는 self-etching primer를 적용하지 않았다. 1, 2, 3군의 치아를 gutta-percha와 AH26 sealer를 사용하여 continuous wave 충전법으로 충전한 후 치근단공 주위 3mm를 제외한 치근면에 nail polish를 2회 도포하였고, 4군(음성 대조군)은 치근면 전체에 도포하였다. 1군과 2군에서 각각 2개의 치아는 주사전자현미경적 관찰을 위해 준비하였다. 모든 치아를 Methylene Blue 수용액에 48시간동안 침적, 수세한 후 치아 장축에 평행하게 양분하여, 10배의 실물확대현미경 하에서 치근단 색소 침투를 관찰하였다. Self-etching primer를 도포한 군과 도포하지않은 군 사이에는 평균 미세누출량에 유의한 차이가 없었다. 주사바늘로 적용한 군과 paper point로 적용한 군 사이에도 평균 미세누출량에 유의한 차이가 없었다. 상아질과 근관sealer계면의 주사전자현미경 관찰 결과 일부분에서 긴밀한 접착 관계를 나타내었고, 다른 부분에서는 간극을 나타내었다.