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PPARα/β agonist MHY2013 attenuates age-associated renal fibrosis
( Sugyeong Ha ),( Ki Wung Chung ),( Hee Jin Jung ),( Seong Min Kim ),( Hye Jin An ),( Hyung Ryoung Moon ),( Hae Young Chung ) 한국장기요양학회 2018 한국장기요양학회 추계학술대회자료집 Vol.2018 No.-
Recent studies have reported that maintaining adequate lipid metabolism is important in the prevention of renal fibrosis during aging. Although PPARα/β activation has been shown to give beneficial effects on age-associated renal changes, the effects of PPARα/β activation on age-associated renal fibrosis have not been investigated yet. Here, we show PPARα/β activator, MHY2013, can significantly alter lipid metabolism in NRK52E renal tubule epithelial cells and attenuate renal fibrosis in aged rat. We found that MHY2013 increased activity of PPARα/ β in NRK52E cells. The increased PPARα/β activity also exerted increase in FAO-associated PPARα/β target genes. In addition, TGF-β and oleic acid-induced lipid accumulation and fibrosis associated gene expression were decreased by MHY2013 in NRK52E cells. To evaluate the effects of MHY2013 on age-associated renal fibrosis, MHY2013 was per-orally administered in aged rat. MHY2013 increased PPARα/β activation and reduced renal lipid accumulation in aged kidney. Furthermore, renal fibrosis was significantly decreased by MHY2013 indicating importance of lipid metabolism on age-associated renal fibrosis. Decrease of fibrosis was followed by attenuation of inflammation in aged rat. Taken together, our results suggest that activation of PPARα/β signaling during aging alleviates abnormal lipid accumulation and reduces age-associated renal fibrosis.
Sugyeong Ha,Ki Wung Chung,Jaewon Lee,Hae Young Chung,Hyung Ryong Moon 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.3
Renal fi brosis is defi ned by excessive extracellularmatrix (ECM) accumulation and is associated witha decreased kidney function. Increased infl ammation andinfi ltration of infl ammatory cells are the key features ofrenal fi brosis development; however, the mechanism ofhow infl ammation starts is still un-known. Here, we showthat the activation of epithelial Protease-activating receptor2 (PAR2) signaling plays an important role in the initiationof infl ammation via increased chemokine expression andinfl ammatory cell induction. In the adenine diet-inducedrenal fi brosis mouse model, PAR2 expression was signifi -cantly increased in the renal tubule region. Kidneys fromPAR2-knockout mice were protected from adenine dietinducedrenal fi brosis, kidney dysfunction, and infl ammation. Using NRK52E kidney epithelial cells, we furtherelucidated the mechanisms underlying these processes. Activation of PAR2 signaling pathway by PAR2 agonistspecifi cally increased the levels of chemokines, includingMCP1 and MCP3, via the MAPK-NF-κB signaling pathway. Inhibition of the MAPK signaling pathway attenuated PAR2agonist-induced NF-κB activation, chemokine expression, and macrophage cell induction. Furthermore, PAR2 activationdirectly increased mesenchymal cell markers in epithelialcells. Taken together, we found that increased PAR2expression and the PAR2/MAPK signaling pathway promoterenal fi brosis by increasing the infl ammatory responses andpromoting EMT process.
Catalytic Reduction of ortho- and meta-Nitroaniline by Nickel Oxide Nanoparticles
( Sugyeong Jeon ),( Jeong Won Ko ),( Weon Bae Ko ) 한국고무학회 2020 엘라스토머 및 콤포지트 Vol.55 No.3
Nickel oxide (NiO) nanoparticles were synthesized by a reaction of nickel nitrate hexahydrate (Ni(NO<sub>3</sub>)<sub>2</sub>ㆍ6H<sub>2</sub>O) and sodium hydroxide (NaOH). The synthesized NiO nanoparticles were examined with X-ray diffraction, scanning electron microscopy, Raman spectroscopy, and ultraviolet-visible (UV-vis) spectroscopy. The NiO nanoparticles were used as the catalyst for the reduction of o- and m-nitroaniline to phenylenediamine. The reduction rate of m-nitroaniline was faster than that of o-nitroaniline. The reduction rate for both o- and m-nitroaniline increased as the reaction temperature increased. The rate of reduction for nitroaniline followed a pseudo first-order reaction rate law.
민수경 ( Sugyeong Min ),김정용 ( Jungyong Kim ),김성찬 ( Sung Chan Kim ) 한국액체미립화학회 2016 한국액체미립화학회 학술강연회 논문집 Vol.2016 No.-
The present study has been conducted to quantify the spray angle which is a common parameter for sprinkler spray analysis. Imaging processing technique based on the laser visualization is compared with measurement of spray patterns by momentum signal using the piezo-electric sensor. A DPSS laser with 100 mW power and 532 nm wave length was utilized for the visualization of sprinkler spray and the outer spray angle was measured to 145º for the reference operating condition of sprinkler. A spray patternator using momentum signal of spray droplets was made with the piezoelectric sensor array and the diameter of the individual sensor is 20 mm. The measured spray angle using the spray patternator with piezo-electric sensor is ranged about 140°~150° and shows good agreement with that of the laser visualization technique. This study can contribute to understanding the spray characteristics of sprinkler head and utilizing the fire safety analysis with sprinkler activation.
Hong, Sugyeong,Go, Yoo Kyung,Derrick, Jeffrey S.,Han, Sanghun,Kim, Jin,Lim, Mi Hee,Kim, Sun Hee American Chemical Society 2018 Inorganic chemistry Vol.57 No.20
<P>Although there has been extensive effort to develop chemical regulators, progress has been static, in part because of these regulators’ unclear mechanisms. Here, we report using advanced electron paramagnetic resonance (EPR) spectroscopy to obtain the first molecular-level structural information regarding a ternary complex of Cu<SUP>II</SUP>-amyloid-β (Aβ) with a chemical regulator that can specifically modulate Cu-induced Aβ aggregation. Our advanced EPR spectroscopic results revealed that a chemical regulator (<B>1</B>) for Cu<SUP>II</SUP>-Aβ<SUB>1-16</SUB> disrupted the coordination environment of Cu<SUP>II</SUP> in Aβ, resulting in the detachment of the primary amine at the N-terminal and a carbonyl group between Asp1 and Ala2 from the Cu<SUP>II</SUP> center and the subsequent formation of a ternary complex, chemical regulator-Cu<SUP>II</SUP>-Aβ<SUB>1-16</SUB>. Therefore, our results demonstrate how a chemical regulator interacts with metal-Aβ at the molecular level. These findings provide novel insight into working mechanisms and thereby contribute to the establishment of a rational design for chemical regulators of metal-Aβ complexes.</P><P>The first molecular-level structural information on a ternary complex of Cu<SUP>II</SUP>−amyloid-β with a chemical regulator was revealed by advanced electron paramagnetic resonance spectroscopy.</P> [FIG OMISSION]</BR>
Aesculetin의 항산화 활성과 MMP-9 활성 억제를 통한 암세포 침윤 억제
홍수경(Sugyeong Hong),김문무(Moon-Moo Kim) 한국생명과학회 2016 생명과학회지 Vol.26 No.6
최근에 종양을 예방하거나 치료하기 위하여 안전하고 효과적인 항암화합물의 개발이 절실하게 요구되고 있다. 그 중에서 전통약재로부터 유래된 천연화합물은 항암후보소재로 관심의 대상이 되어왔다. 본 연구에서 사용된 aesculetin은 약용식물로 널리 알려진 산초나무의 주요 성분이다. Aesculetin은 항염증 및 항균과 같은 다양한 생물학적 효과를 가진다고 보고되었다. 그러나 세포침윤과 관련된 효과는 아직 발견되지 않았다. 그러므로 본 연구에서는 사람섬유아육종세포(HT1080)에서 항산화와 기질금속단백질분해효소(MMPs)에 대한 aesculetin의 효과를 조사하였다. 항산화 효과에 대한 연구에서 aesculetin은 DPPH radical에 대한 소거능뿐 만 아니라 환원력이 우수한 것으로 나타났다. 우선, MTT 실험을 이용하여 HT1080세포에서 aesculetin의 2 μM 이하의 농도에서 독성이 없는 것으로 나타났다. MMP-2와 MMP-9의 활성과 단백질 발현 수준에 대한 aesculetin의 억제효과는 gelatin zymography와 western blot을 이용하여 조사되었다. Aesculetin은 세포침윤과 관련된 MMP-9의 활성의 억제효과가 있는 것으로 나타났다. 더욱이, aesculetin은 TIMP-1의 단백질 발현 수준을 증가시켰으나, PMA로 자극된 MMP-9의 단백질 발현 수준을 감소시켰다. 더불어 aesculetin은 농도의존적으로 암전이와 관련된 세포침윤을 현저하게 억제하였다. 위의 결과들을 바탕으로, aesculetin은 세포침윤과 관련된 MMP의 활성과 발현의 억제를 통해 세포침윤을 예방할 수 있는 소재로서 기대된다. The development of safe and effective anti-cancer compounds has been seriously required to prevent and treat development of tumor in recent years. Among them, natural compounds derived traditional medicinal stuffs have been paid to attention as an anti-cancer candidate. In this study, aesculetin is a main component of a widely known as a medicinal stuff. It was reported that aesculetin has various biological effects such as anti-inflammatory and anti-bacterial, but its effect related to cell invasion was not discovered. Therefore, in this study, the effect of aesculetin on antioxidant and matrix metalloproteases (MMPs) was investigated in human fibrosarcoma cells, HT1080. First of all, aesculetin showed the scavenging activity of DPPH radical and reducing power in a dose dependent manner. As a result of cytotoxicity, the nontoxic concentration of aesculetin was below 2 μM in HT1080 cells performed by MTT assay. In addition, aesculetin displayed the inhibitory effect on MMP-9 activity related to cell invasion in experiment carried out by gelatin zymography assay. Furthermore, aesculetin increased the expression level of TIMP-1 but decreased the expression level of MMP-9 stimulated with PMA in western blot assay. Furthermore, aesculetin remarkably inhibited cell invasion related to metastasis a dose dependent manner. Above results suggest that aesculetin could exert chemopreventive effect through inhibition of activity and expression of MMP-9 related to cell invasion.