Keratin 17 identified by proteomic analysis may be involved in tumor angiogenesis

( Yong Xu ),( Su Zhen Zhang ),( Can Hua Huang ),( Xin Yu Liu ),( Zhen Hua Zhong ),( Wen Li Hou ),( Zi Fen Su ),( Yu Quan Wei ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.6

The Lung Function Impairment in Non-Atopic Patients With Chronic Rhinosinusitis and Its Correlation Analysis

Linghao Zhang,Lu Zhang,Chun-Hong Zhang,Xiao-Bi Fang,Zhen-Xiao Huang,Qing -Yuan Shi,Li-Ping Wu,Peng Wu,Zhen-Zhen Wang,Zhi-Su Liao 대한이비인후과학회 2016 Clinical and Experimental Otorhinolaryngology Vol.9 No.4

Objectives. Chronic rhinosinusitis (CRS) is common disease in otorhinolaryngology and will lead to lower airway abnormality. However, the only lung function in CRS patients and associated factors have not been much studied. Methods. One hundred patients with CRS with nasal polyps (CRSwNP group), 40 patients with CRS without nasal polyps (CRSsNP group), and 100 patients without CRS were enrolled. The difference in lung function was compared. Meanwhile, CRSwNP and CRSsNP group were required to undergo a bronchial provocation or dilation test. Additionally, subjective and objective outcomes were measured by the visual analogue scale (VAS), 20-item Sino-Nasal Outcome Test (SNOT-20), Lund-Mackay score, Lund-Kennedy endoscopic score. The correlation and regression methods were used to analyze the relationship between their lung function and the above parameters. Results. The forced expiratory volume in 1 second (FEV1) and forced expiratory flow between 25% and 75% of forced vital capacity (FEF25-75) of CRSwNP group were significantly lower than other groups (P<0.05). On peak expiratory flow, there was no difference between three groups. In CRSwNP group, FEV1 was negatively correlated with peripheral blood eosinophil count (PBEC) and duration of disease (r=–0.348, P=0.013 and r=–0.344, P=0.014, respectively), FEF25-75 negatively with VAS, SNOT-20 (r=–0.490, P=0.028 and r=–0.478, P=0.033, respectively) in CRSsNP group. The incidence of positive bronchial provocation and dilation test was lower in CRSwNP group (10% and 0%, respectively), with both 0% in CRSsNP group. The multiple linear regression analysis indicated that change ratio of FEV1 before and after bronchial provocation or dilation test were correlated with PBEC in CRSwNP group (β=0.403, P=0.006). Conclusion. CRS leading to impaired maximum ventilation and small airway is associated with the existence of nasal polyp. Lung function impairments can be reflected by PBEC, duration, VAS, and SNOT-20. In CRSwNP patients, PBEC is independent predictor of FEV1 change ratio.

Mocklinter : Linting Mutual Exclusive Deadlocks with Lock Allocation Graphs

Zhen Yu,Xiaohong Su,Tiantian Wang,Peijun Ma 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.3

Deadlocks are serious runtime bugs and are difficult to expose, reproduce and diagnose. Once suffering from them, programs may be afflicted with increasing response time, decreasing throughputs, or even crashes. We present Mocklinter, a dynamic deadlock detection tool to capture a deadlock as soon as it happens and spit out enough information to support source-level debugging. Mocklinter tracks the synchronization state of the target program by dynamically constructing and maintaining a lock allocation graph. Mocklinter uses this graph to decide whether a deadlock is confronted or not. Mocklinter handles all types of pthread mutexes and can detect any number of deadlocks at a time. Each deadlock captured by Mocklinter can involve any number of threads. We implemented Mocklinter in Linux-3.2.0 and evaluated it with ten applications, including Dining-Philosophers, Sshfs, SQLite, OpenLDAP, MySQL and so on, whose sizes varies from 0.1K to 1021.0K in terms of LOC. The results demonstrate effectiveness against real or artificial deadlock bugs, while incurring modest performance overhead and scaling to more than one thousand of threads.

P035 : Carpal tunnel syndrome in Behcet’s disease

( Su Hyun Cho ),( Do Young Kim ),( Min Ju Choi ),( Zhen Long Zheng ),( Hoon Park ),( Dong Sik Bang ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Behcet`s disease (BD) is a chronic inflammatory disease characterized by orogenital ulcers, skin and ocular lesions, in addition to articular, vascular, and neurologic symptoms. Carpal tunnel syndrome (CTS), a peripheral neuropathy caused by compression of the median nerve, can also occur in BD patients secondary to inflammation in the connective tissues, vessels, and tendons, as well as nerve involvement in BD itself. However, reports of patients who have CTS in BD are rare. Objectives: The aim of this study was to evaluate the clinical characteristics of CTS in BD patients. Methods: Retrospective analysis of the medical records of 14 BD patients who were diagnosed with CTS was performed at the BD Specialty Clinic of Severance Hospital. Patient demographics, disease activity/severity for both diseases, and the clinical characteristics of CTS in BD were recorded and analyzed. Results: All 14 BD patients with CTS were women. Twelve patients (85.7%) were diagnosed with active BD. The CTS was mild in 8 patients (57.2%), moderate in 3 patients (21.4%), and severe in 3 patients (21.4%). Ten patients (71.4%) had BD prior to the diagnosis of CTS, and these 10 patients all had active BD. Conclusion: CTS can occur as a result of the inflammation associated with BD and can also be the presenting symptom of nerve involvement in BD. Therefore, a higher degree of suspicion should be maintained for CTS in patients with BD and vice versa; however, the exact relationship is uncertain.

Time-dependent Optimal Heater Control in Thermoforming Preheating Using Dual Optimization Steps

Zhen-Zhe Li,Kwang-Su Heo,Seoug-Yun Seol 한국정밀공학회 2008 International Journal of Precision Engineering and Vol.9 No.4

Thermoforming is one of the most versatile and economical processes available for shaping polymer products, but obtaining a uniform thickness of the final product using this method is difficult. Heater power adjustment is very important because the thickness distribution depends strongly on the distribution of the sheet temperature. In this paper; the steady-state optimum distribution of heater power is first ascertained by a numerical optimization to obtain a uniform sheet temperature. The time-dependent optimal heater input is then determined to decrease the temperature difference through the direction of the thickness using the response surface method and the D-optimal method. The optimal results show that the time-dependent optimum heater power distribution gives an acceptable uniform sheet temperature in the forming temperature range by the end of the heating process.

Calculation the Loss Factor of the Partial Constrained Layer Damping

Su Suying,Gong Zhen,Kang Feng Hua,Shen Feng 대한기계학회 1997 Asia-Pacific Vibration Conference Vol.1 No.1

A morphological study on autumnal morphs of Aphis glycines (Hemiptera: Aphididae)

Zhen-qi Tian,Su-ji Wang,Bing Bai,Jian Liu,Kuijun Zhao 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is one of the most important pests of soybean. The complex life cycle of A. glycines is characterized as heteroecious and holocyclic, and has seasonal polymorphisms occurring during its life cycle. In the autumn, A. glycines occur as winged and wingless virginoparae in soybeans, as gynoparae and males that migrate from soybeans to Rhamnus spp. and as gynoparae, males and oviparae on Rhamnus spp. In this study, wingless virginoparae, gynoparae, males and oviparae of A. glycines were successfully induced in the laboratory and morphological parameters of these morphs were selected for quantitation. To aid in identification of these A. glycines autumnal morphs, these aphids were imaged by microscope and significant differences in morphological characteristics were found: distal parts of hind leg femurs of gynoparae were grayish black, which were darker than those of winged virginoparae; the 4th instars of gynoparae, males and winged virginoparae differ greatly in dorsal abdomen coloration and covering. Our results provide an important guide for distinguishing adults of gynoparae and winged virginoparae, and for identifying nymphs of gynoparae, males and winged virginoparae.

Comparison of Two Site-Specifically ¹⁸F-Labeled Affibodies for PET Imaging of EGFR Positive Tumors

Su, Xinhui,Cheng, Kai,Jeon, Jongho,Shen, Bin,Venturin, Gianina Teribele,Hu, Xiang,Rao, Jianghong,Chin, Frederick T.,Wu, Hua,Cheng, Zhen American Chemical Society 2014 MOLECULAR PHARMACEUTICS Vol.11 No.11

<P>The epidermal growth factor receptor (EGFR) serves as an attractive target for cancer molecular imaging and therapy. Our previous positron emission tomography (PET) studies showed that the EGFR-targeting affibody molecules <SUP>64</SUP>Cu-DOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-FBEM-Z<SUB>EGFR:1907</SUB> can discriminate between high and low EGFR-expression tumors and have the potential for patient selection for EGFR-targeted therapy. Compared with <SUP>64</SUP>Cu, <SUP>18</SUP>F may improve imaging of EGFR-expression and is more suitable for clinical application, but the labeling reaction of <SUP>18</SUP>F-FBEM-Z<SUB>EGFR:1907</SUB> requires a long synthesis time. The aim of the present study is to develop a new generation of <SUP>18</SUP>F labeled affibody probes (Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB>) and to determine whether they are suitable agents for imaging of EGFR expression. The first approach consisted of conjugating Z<SUB>EGFR:1907</SUB> with NOTA and radiolabeling with Al<SUP>18</SUP>F to produce Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB>. In a second approach the prosthetic group <SUP>18</SUP>F-labeled-2-cyanobenzothiazole (<SUP>18</SUP>F-CBT) was conjugated to Cys-Z<SUB>EGFR:1907</SUB> to produce <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB>. Binding affinity and specificity of Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> to EGFR were evaluated using A431 cells. Biodistribution and PET studies were conducted on mice bearing A431 xenografts after injection of Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> or <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> with or without coinjection of unlabeled affibody proteins. The radiosyntheses of Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> were completed successfully within 40 and 120 min with a decay-corrected yield of 15% and 41% using a 2-step, 1-pot reaction and 2-step, 2-pot reaction, respectively. Both probes bound to EGFR with low nanomolar affinity in A431 cells. Although <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> showed instability <I>in vivo</I>, biodistribution studies revealed rapid and high tumor accumulation and quick clearance from normal tissues except the bones. In contrast, Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> demonstrated high <I>in vitro</I> and <I>in vivo</I> stability, high tumor uptake, and relative low uptake in most of the normal organs except the liver and kidneys at 3 h after injection. The specificity of both probes for A431 tumors was confirmed by their lower uptake on coinjection of unlabeled affibody. PET studies showed that Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> could clearly identify EGFR positive tumors with good contrast. Two strategies for <SUP>18</SUP>F-labeling of affibody molecules were successfully developed as two model platforms using NOTA or CBT coupling to affibody molecules that contain an N-terminal cysteine. Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> can be reliably obtained in a relatively short time. Biodistribution and PET studies demonstrated that Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> is a promising PET probe for imaging EGFR expression in living mice.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mpohbp/2014/mpohbp.2014.11.issue-11/mp5003043/production/images/medium/mp-2014-003043_0008.gif'></P>

Anti-tumor Efficacy of a Hepatocellular Carcinoma Vaccine Based on Dendritic Cells Combined with Tumor-derived Autophagosomes in Murine Models

Su, Shu,Zhou, Hao,Xue, Meng,Liu, Jing-Yu,Ding, Lei,Cao, Meng,Zhou, Zhen-Xian,Hu, Hong-Min,Wang, Li-Xin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

The majority of hepatocellular carcinoma (HCC) patients have a poor prognosis with current therapies, and new approaches are urgently needed. We have developed a novel therapeutic cancer vaccine platform based on tumor cell derived autophagosomes (DRibbles) for cancer immunotherapy. We here evaluated the effectiveness of DRibbles-pulsed dendritic cell (DC) immunization to induce anti-tumor immunity in BALB/c mouse HCC and humanized HCC mouse models generated by transplantation of human HCC cells (HepG2) into BALB/c-nu mice. DRibbles were enriched from H22 or BNL cells, BALB/c-derived HCC cell lines, by inducing autophagy and blocking protein degradation. DRibbles-pulsed DC immunization induced a specific T cell response against HCC and resulted in significant inhibition of tumor growth compared to mice treated with DCs alone. Antitumor efficacy of the DCs-DRibbles vaccine was also demonstrated in a humanized HCC mouse model. The results indicated that HCC/DRibbles-pulsed DCs immunotherapy might be useful for suppressing the growth of residual tumors after primary therapy of human HCC.

Calculation the Loss Factor of the Partial Constained Layer Damping

Su Suying,Gong Zhen,Kang Fenghua,Feng Shen 한국소음진동공학회 1997 한국소음진동공학회 국제학술대회논문집 Vol.1997 No.1