상수처리 : F/A 여과지의 맛,냄새물질 제거효과

이경희 ( Kyeung Hee Lee ),정은재 ( Eun Jae Jeong ),김대수 ( Dae Su Kim ),김유선 ( Yoo Sun Kim ),나진영 ( Jin Young Na ),신창수 ( Chang Su Shin ) 한국물환경학회 ( 구 한국수질보전학회 ) 2009 공동 추계학술발표회 Vol.2009 No.-

Development of EST SSR-Markers to study genetic diversity in Foxtail Millet (Setaria italica)

Jae Young Song,Yu-Mi Choi,Su Kyeung Lee,Gi-An Lee,Jae-Gyun Gwag,Myung-Chul Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Foxtail millet (Setaria italica L.) is the second most widely cultivated species of millet, especially in East Asia and is a tractable experimental model crop for studying functional genomics of millets. However, insufficient researches had been conducted about the foxtail millet germplasm and is significantly impeding its genetic improvement. We attempted to develop EST-derived-SSR (eSSR) markers and utilize them in genetic comparison of germplasm and transferability. A total of 66,027 foxtail millet EST sequences and 42,754 genomic sequence were deduced transcriptom. Approximately 42,000 single tone contigs were generated using DNAstar 5.0 software for redundancy minimization. Nearly 33% of the 14,012 unigenes contained SSRs, but primers were designed for a total of 314 microsatellites concentrating with more than 24 bp of repeats. A total of 314 primers were successfully designed with more than 24 bp of repeats. From these microsatellites, 56 primer pairs were showed polymorphism with over than 15 bp differences among 96 accessions collected from different countries. Polymorphic information content (PIC) value ranged from 0.020 to 0.700 with an average of 0.381 indicating moderate level of informativeness within these EST-SSRs markers. The EST-SSR markers developed in this study will serve as a useful source for genetic studies, such as genetic variability, transferability, association mapping, and molecular breeding

Elimination of Chrysanthemum stunt viroid (CSVd) from an Viroidinfected Chrysanthemum through Shoot Tip Culture

Su-Min Jeon,Wina Dian Savitri,Kyeung-Il Park,Mi-Young Chung,Chang-Kil Kim 한국화훼산업육성협회 2012 화훼연구 Vol.20 No.4

As the increase of chrysanthemum demand on chrysanthemum increases in Korea, the production of high quality chrysanthemum is needed. Chrysanthemum stunt viroid (CSVd) is one of the important viroid, which infects chrysanthemum and induces diseases that affects the decrease of quality and yield. To solve this problem, we used different size of meristem of chrysanthemum ‘Ency’ for shoot tip culture and also that of combined with heat treatment at 37οC. The efficiency of CSVd elimination was influenced by the size of shoot tip. The small-sized of meristems with 1 or 2 leaf primodia were regenerated into the highest number of CSVd-free plantlets. By RT-PCR, the 214-bp band corresponding to CSVd was not detected in 22.2% of the total number of tested regenerants from shoot tips with 2 leaf primordia. While, shoot tip culture combined with heat treatment of one-month-old in vitro shoots was not effective for CSVd-elimination. The CSVd-free plants grew more vigorously than CSVd-infected plants in the greenhouse.

Effect of Forest Seed Endophytes on Seeds Germination of Echinochloa crus-galli P. Beauv. and Kummerowia striata (Thunb.) Schindl

Kyeung Min HAN,Kyeong Min KIM,Sea Hyun KIM,Yeong-Su KIM,Chae Sun NA 한국생물공학회 2022 한국생물공학회 학술대회 Vol.2022 No.9

A preliminary investigation into the decomposition rate of wild boar carcasses in forest habitats

Hee-Kyeung Cho,Eu-Tteum Kim,Bong-Su Jung,Son-Il Pak 한국예방수의학회 2021 예방수의학회지 Vol.45 No.1

The estimation of the postmortem interval (PMI, the time that has elapsed since the death) is a critical issue for the biosecurity enforcement officers who implement to the timely establishment of biosecurity zone for preventing susceptible animals from disease transmission given the rapid occurrence of an infectious disease. Increasing attention has been paid to PMI of wild boar (Sus scrofa) carcasses associated with African swine fever epidemics in Korea since October 2019 to explain the geographical transmission of the disease, as well as to provide potential target animals for prevention measures in terms of farm biosecurity. This study is, to the best of our knowledge, the first to describe the decomposition process of wild boars in different microhabitats in the country. In the present comparative study, we obtained field data from the decomposition process of the wild boar and domestic pig carcasses continuously exposed aboveground in natural environment settings allowing animal scavenging. This study compared the pattern of decomposition in 16 wild boar carrion and 10 domestic pig carcasses placed between August and November 2019. Quite differences in decomposition rate measured by total body score and tissue s gross morphology over time were observed between wild boars and domestic pigs. Overall, the wild boar decomposed much more slowly than the domestic pig throughout the end of the experimental period. In addition, color changes to the skin were easily seen in domestic pigs, whereas there is much variation in the wild boar, especially carcasses placed in late autumn. Moreover, some wild boar carcasses did not show any sign of bloating. These results indicate that decomposition rates derived from forensic taphonomic studies on domestic pigs may be not directly applicable to the wild boar, hence there is a need to develop regional decomposition models to be employed in different geographical situations to increase the accuracy of PMI of wild boar carcasses.

Fishy Smells as Potent Attractants for the Bean Bug, Riptortus clavatus (Thunberg) (Hemiptera: Alydidae)

Kim Su-Joung,Huh Hye-Soon,Han Kyeung-Sik,Park Chung-Gyoo 한국응용곤충학회 2004 한국응용곤충학회 학술대회논문집 Vol.2004 No.1

참죽나무 열매의 항산화 및 Elastase, Collagenase, Hyaluronidase, α-Glucosidase 저해 효과

임수빈(Su-Bin Lim),김명욱(Myung-Uk Kim),이은호(Eun-Ho Lee),김예진(Ye-Jin Kim),조은비(Eun-Bi Cho),박경일(Kyeung-Il Park),강인규(In-Kyu Kang),조영제(Young-Je Cho) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.11

본 연구에서는 참죽나무 열매 추출물을 이용하여 항산화 및 elastase, collagenase 및 hyaluronidase 등의 효소활성 저해 효과를 평가하였다. 참죽나무 열매 추출물의 total phenolic contents(TPC)는 50% 에탄올 추출물에서 104.94 ㎎/g으로 가장 높은 추출 수율을 나타내었으며 열수 추출물의 경우 56.54 ㎎/g의 추출 수율을 나타내었다. 이를 동결건조 한 분말(Cedrela sinensis powder; CSP)의 TPC 함량은 390.73, 491.47 ㎎/g으로 높은 TPC 함량을 나타내었다. CSP의 항산화 효과를 측정한 결과 DPPH 라디칼 소거 활성은 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 72.17%와 71.38%의 소거 활성을 나타내었다. ABTS 라디칼 소거 활성을 측정한 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 99.11%와 99.16%의 소거 활성을 나타내었다. PF를 측정한 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 1.75와 1.91 PF를 나타내었고, TBARS는 200 ㎍/mL 농도에서 CSWP는 38.72%, CSEP는 56.95%를 나타내었다. DPPH와 ABTS 라디칼 소거 활성은 positive control로 사용한 BHT보다 높은 활성을 나타내었으나, PF와 TBARS의 경우 BHT보다 낮은 효과를 나타내었다. CSP의 elastase 저해 효과를 측정한 결과 200 ㎍/mL 농도에서 CSEP에서 85.28%를 나타내었다. Collagenase 저해 효과를 측정한 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 29.38%, 56.66%의 저해 효과를 나타내었다. 따라서 CSP는 elastase와 collagenase가 관여하는 주름 생성과정을 억제할 수 있을 것으로 예상되었다. CSP의 hyaluronidase 저해 효과를 측정한 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 87.80%, 88.51%의 저해 효과를 나타내었다. α-Glucosidase 저해 효과를 측정해본 결과 200 ㎍/mL 농도에서 CSWP와 CSEP가 각각 92.17%, 99.06%로, positive control로 사용한 EGCG와 유사하거나 더욱 높은 저해 효과를 나타내었다. 이상의 연구 결과를 통해 참죽나무 열매의 동결건조물은 항산화 효과, 주름개선 효과, 항염증 효과 및 α-glucosidase 저해를 통한 혈당상승 억제효과가 우수한 것으로 판단되었으며, 기능성 소재로서의 활용 가능성이 높다고 판단되었다. This study examined the anti-oxidation and inhibitory activities of extracts from Cedrela sinensis fruits on elastase, collagenase, hyaluronidase, and α-glucosidase. The total phenolics content (TPC) of C. sinensis fruits was 56.54 ㎎/g in the hot water extracts and 104.94 ㎎/g in the 50% ethanol extracts, which was the highest. The TPC in the freeze-dried powder of the hot water extracts and the 50% ethanol extracts were 390.73 and 491.47 ㎎/g, respectively. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of the C. sinensis fruits water extract powder (CSWP) and C. sinensis fruits ethanol extract powder (CSEP) were 72.17 and 71.38% at a 200 ㎍/mL concentration, respectively. The 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization of CSWP and CSEP were 99.11 and 99.16%, respectively, at 200 ㎍/mL. The anti-oxidant protection factors (PF) of CSWP and CSEP were 1.75 and 1.91 PF, respectively, at 200 ㎍/mL. The thiobarbituric acid reactive substances (TBARS) of CSWP and CSEP were 38.72% and 56.95%, respectively, at 200 ㎍/mL. The DPPH and ABTS of CSWP and CSEP were higher than those of butylated hydroxytoluene (BHT), but PF and TBARS were lower than those of BHT. The elastase inhibitory activity of CSWP was not observed but the inhibitory activity of CSEP was 85.28% at 200 ㎍/mL. The collagenase inhibitory activity of CSWP and CSEP were 29.38% and 56.66%, respectively, at 200 ㎍/mL. Therefore, CSP was considered to be a potential source of cosmetic products for the wrinkle-improving function. The hyaluronidase (HAase) inhibitory activity of CSWP and CSEP as the biological activity for functional food were 87.80% and 88.51%, respectively, at 200 ㎍/mL. The α-glucosidase inhibitory activity of CSWP and CSEP were 92.17% and 99.06%, respectively, at 200 ㎍/mL. CSWP and CSEP were similar or more effective than EGCG as a positive control. The CSP showed excellent of anti-oxidant, anti-wrinkle, and anti-inflammation activities and helped decrease the blood sugar content through the glucosidase inhibition activity. These results show that extracts from C. sinensis can be used as a functional resource with anti-oxidant, anti-wrinkle, anti-inflammation, and anti-diabetes activities.

합성수지 점토를 이용한 심장발생 모델링 실습 방법 제안

주경민(Kyeung Min Joo),전수경(Su Kyoung Jeon),권혁이(Hyeok Yi Kwon),황영일(Young-il Hwang) 대한체질인류학회 2021 해부·생물인류학 (Anat Biol Anthropol) Vol.34 No.4

심장은 복잡한 과정을 거쳐 발생하기 때문에 그에 대한 공간적 이해가 매우 어렵다. 서울대학교 의과대학에서는 심장발생 과정의 이해를 촉진하고자 발생 과정에 대한 모델링 실습을 고안하고 2011년부터 의학과 1학년을 대상으로 이를 시행해 오고 있다. 본 실습의 타당성을 알아보고, 각 의과대학에 이 과정의 시행을 제안하고자 이 연구를 시행하였다. 실습에는 시중에서 판매되는 어린이용 합성수지 점토를 이용하였다. 실습 첫날에는 유출로, 오른심실, 왼심실을 한 단위로, 공통심방을 한 단위로 각각 만든 후 이 두 단위를 이어 붙여 발생 30일 또는 35일의 심장을 만들도록 하였다. 2~3일 동안 모형이 마르게 둔 후에 제작된 심장을 유출로, 오른심실, 왼심실을 관통하는 관상단면을 따라 절단한 후 속 모습을 교과서의 모습과 비교하고 성찰하도록 하였다. 이후 색이 다른 점토를 이용하여 방실관, 심실사이구멍, 유출로 등에 심장속막방석이 자라는 모습을 재현하여 심장의 4개 방, 대동맥, 허파동맥이 분할되는 모습을 표현하도록 하였다. 실습에 대한 학생들의 인식은 전반적으로 긍정적이어서, 실습은 그다지 어렵지 않고 재미있었으며 학습에 도움이 되었고 적극적으로 참여하였다고 하였다. 또한 동료들과의 토론은 실습을 진행하고 심장발생 과정을 이해하는 데 도움이 되었다고 하였다. 실습 시행 전인 2008~2010년도와 실습 시행 후인 2011~2013년도 사이의 학기말에 실시한 시험에서 심장발생 관련 문항과 그 외 문항의 정답률을 비교하여 보았을 때 각 연도별로 두 문항군의 정답률 사이에 통계적으로 유의한 결과는 나타나지 않았다. 그러나 두 문항군의 정답률 비율은 실습 시행 이후에 다소 향상된 양상을 보였다. 이상의 결과를 바탕으로 보았을 때, 2차원 자료로 이해하기 어려운 심장실습에 대해서 점토를 이용한 3차원 모델링 실습은 여러 가지 측면에서 권장할 만한 것으로 판단된다. Because of the complexity of the heart development process, a 3-dimensional understanding of it is not easy. SNU College of Medicine devised a modeling exercise for the developing heart to promote understanding of the process and implemented it for first-year students since 2011. This study was performed to validate this exercise and propose the implementation of this at each medical school. We used commercially available polymer clay for children. On the first day, students made the outflow tract and both ventricles as a unit and the common atrium as another unit. These two units were combined to form an embryonic 30-day or 35-day developing heart model. After leaving the model to dry for two to three days, a coronal section was made through the outflow tract and both ventricles to open the model. Students compared the interior of the model to that depicted in the textbook and reflected on it. Afterward, students marked with colored clay the appearance of the endocardial cushion along the atrioventricular canal, interventricular septum, and the outflow tract, simulating the division of the four chambers and the outflow tract into the aorta and the pulmonary trunk. The students’ perception of the exercise was generally positive, in that it was not difficult to perform, fun, helpful in learning, and they actively participated. They also responded that discussions with colleagues helped them perform the modeling and understand the developing processes of the heart. We analyzed the scores of the embryology exam at the end of the semester and compared those of the years 2008~2010 and 2011~2013, corresponding years before and after the practice, respectively. There was no statistically significant difference between the correct answer rates of the questions concerning heart development and that of the other questions in all years. However, even though not significant statistically, the ratio of correct answers in the two question groups showed some improvement after the exercise. In conclusion, we recommend three-dimensional modeling exercise using polymer clay for several reasons for a 3-dimensional understanding of heart development, which is difficult to achieve based on 2-dimensional materials.

기능성 식품소재 개발을 위한 야생 곰취잎 추출물의 생리활성

임수빈(Su-Bin Lim),김명욱(Myung-Uk Kim),박경일(Kyeung-Il Park),이은호(Eun-Ho Lee),김예진(Ye-Jin Kim),조은비(Eun-Bi Cho),김병오(Byeong-Oh Kim),조영제(Young-Je Cho) 한국식품저장유통학회 2018 한국식품저장유통학회지 Vol.25 No.3

장애아 어머니의 자아존중감과 불안에 관한 연구

정길수,이경숙 中央醫學社 1994 中央醫學 Vol.59 No.11

This study explored the relationships between self-esteem and anxiety of mothers with handicapped children. The purpose was to contribute to theoretical understanding of the relationships among these two variables and eventually to the planning of nursing care to enhance the life of mothers with handicapped children. Data were collected from July 21 to August 8, 1994 at three handicapped children's private institutes in Daejeon. The subjects were 63 mothers with handicapped children. Anxiety was measured by Zung's Self-rating Anxiety Scale and Self-esteem by Rosenberg's Self-esteem Scale. The data were analyzed by an S.A.S computerized program and included percent ages, frequency, ANOVA and Pearson correlation coefficient. The results of this study are summarized; 1) The total mean score for self-esteem and anxiety of mothers with handicapped children was 27.14, and 40.84 respectively. 2) There were only significant differences according to mother's education(P;.01) and occupation(P<.05) between self-esteem and general characteristics of mothers with handicapped children. Also, there were only significant differences according to mother's education(P<.05), sex of children(P<.05), and children's duration of special education(P<,0l) between anxiety and general characteristics of mothers with handicapped children. 3) There were significant correlation in the relationship between self-esteem and anxiety of mothers with handicapped children(r=-0.5986, P<.001).