디스템파 환견의 혈청단백질에 관한 연구 - 로지크로마토구라피법에 의한 혈청단백질의 이동거리에 관하여 -

한수남,Han Su Nam 대한수의사회 1962 대한수의사회지 Vol.6 No.1

The moving distance of serum protein was determined in the blood of normal, convalescent, and dogs in active progress of the diseas with paper chromatography. The following results were obtained. 1. The moving distance of serum protein in normal dogs were

돼지의 백혈구 인터폐론 생산에 관한 비교연구

한수남,이장락,이창업,Han, Su-nam,Lee, Jang-nag,Lee, Chang-eop 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2

Attempts were to produce porcine leukocyte interferon(PorLeIF) and porcine immune interferon (PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on poreine-derived PK(15) cells, human-derived FL cells, and Korean native black goat-derived BGK cells. The results were summarized as follws: 1. In the isolation of porcine leukocytes, the mean isolation rate by the buffy coat separation method (28.7%) was higher than that by the hydroxyethyl starch-RBC sedimentation method (9.2%). 2. When NDV(BI)-induced PorLeIFs were assyed on PK(15) cells and FL cells, the mean titers were 129 IU/ml and 72 IU/ml respectively, being 55.8% of the activity in homologous species system expressed in heterologous system. 3. The activities of PHA P-induced PorIIFs were 197 IU/ml on PK (15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK (15) cells. 4. The cytopathic effect of vesicular stomatitis virus was observed in BGK cells derived from Korean native black goat kidney permitting interferon assay on the cells. While the cross-species antiviral activity of reference human ${\alpha},\;{\beta}-interferon$ was observed on the cells, PorLeIF and PorIIF did not show any activity.

韓國 殺母蛇 咬傷症의 一般的 考察

Su Nam Han(韓壽南) 한국예방수의학회 1981 예방수의학회지 Vol.5 No.2

The observation on the snakebites caused by the venomous snakes of family Agkistrodon in Korea was reviewed. No statistic data on the snakebites were available. In addition, the antivenins against the responsible snakes venoms are not prepared though the antivenins and a program to prevent the victims of the snakebites are urgently needed. In order to unders and the characterization of some Agkistrodon snake venoms, biochemical and immunoserological studies were carried out.

닭의 호흡기성(呼吸器性) Mycoplasma 균병(菌柄)에 대한 Furamizole 의 예방시험(豫防試驗)

서익수,한수남,Seo, Ik Soo,Han, Su Nam 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2

A number of chemotherapeutic agents, namely antibiotics, sulfonamides and nitrofuran derivatives have been used, as a fred additive, for the purposes of growth improvement of chicken, increase of feed efficiency, decrease of animal mortality, and disease prevention. In these experiments, furamizole which is one of nitrofuran derivatives and feed additive was test, in field, its antibacterial activity against Mycoplasma gallisepticum causing air sac disease, its effect on pullorum disease control, its effect on the lowering the mortality of chicken and finally the increase of feed efficiency. Throughout the studies, furamizole, in concentration of 0.025% in feed fed to baby chicken continuously resulted as following: 1. Tested chicken showed no avian mycoplasma infection compared to 3.7% outbreak in control chicken. 2. Tested chicken showed a low degree of outbreak of pullorum disease. However, its outbreak was much more surpresed compared to that of control chicken. 3. Total mortality rate of 5.5% and 30.8% were obtained in test and control chicken respectively. 4. Feed efficiency were 2.83 and 2.97 in test and control chicken respectively.

Studies on the Production of Porcine Leukocyte Interferon and Porcine Immune Interferon: Its Cross-species Antiviral Activity

태주호,한수남,Tai, Joo-Ho,Han, Su-Nam Korean Society for Biochemistry and Molecular Biol 1987 한국생화학회지 Vol.20 No.2

본 실험에서는 돼지 백혈구 배양에서 Newcastle disease virus(NDV)와 Phytohemagglutinin(PHA)을 사용하여 돼지 백혈구 인터페론 및 돼지 면역 인터페론을 생산하고 돼지유래 PK(15) cell, 사람유래 FL cell 및 산양유래 BGK cell에서 VSV에 대한 항바이러스 역가를 비교조사하였다. NDV로 유발시킨 돼지 백혈구 인터페론의 평균역가는 PK(15)cell에서 129 IU/ml, FL cell에서 72 IU/ml로 55.8%의 교차종간 항바이러스력을 보였다. PHA로 유발시킨 돼지 면역 인터페론의 평균역가는 PK(15) cell에서 197 IU/ml로서 돼지 백혈구 인터페론의 1. 5배였으나 FL cell에서는 항바이러스력이 관찰되지 않았다. 한국재래산양유래 BGK cell에 VSV를 접종한 결과 CPE가 관찰되어 감수성이 인정되었으며 VSV를 이용한 인터페론 assay가 가능하였다. 본 실험에서 생산된 돼지 백혈구 및 연역 인터페론은 BGK cell에서 항바이러스력을 나타내지 않았으나 사람 백혈구 및 섬유아세포 인터페론은 항바이러스력이 관찰되어 사람 인터페론의 광범위한 교차종간 항바이러스력을 확인할 수 있었다. In the present study, attempts were made to produce porcine leukocyte interferon (PorLeIF) and porcine immune interferon (PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on porcine-derived PK(15) cells, human-derived FL cells, and Korean native black goat-derived BGK cells. When Newcastle disease virus(NDV)-induced PorLeIFs were assayed on homologous PK(15) cells, and heterologous FL cells, the mean titers were 129 IU/ml and 72 IU/ml respectively. The activities of phytohemagglutinin(PHA)-induced PorIIFs were 197 IU/ml on PK(15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK(15) cells. BGK cell line derived from Korean native black goat kidney was o bserved to be ahost cell for vesicular stomatitis virus, showing cytopathic effect upon infection. In the cells PorLeIF and PorIIF did not exhibit any antiviral activity, but reference human $\alpha$- and $\beta$-inbterferons exhibited the cross-species antiviral activity, indicating that BGK cell line can be used for human interferon assay.

돼지 백혈구 인터페론 및 돼지 면역 인터페론 생산에 관한 연구 교차종간 항바이러스 효과

태주호,한수남 ( Joo Ho Tai,Su Nam Han ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.2

In the present study, attempts were made to produce porcine leukocyte interferon (PorLeIF) and porcine immune interferon (PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on porcine-derived PK(15) cells, human-derived FL cells, and Korean native black goat-derived BGK cells. When Newcastle disease virus(NDV)-induced PorLeIFs were assayed on homologous PK(15) cells, and heterologous FL cells, the mean titers were 129 IU/㎖ and 72 IU/㎖ respectively. The activities of phytohemagglutinin-(PHA)-induced PorIIFs were 197 IU/㎖ on PK(15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK(15) cells. BGK cell line derived from Korean native black goat kidney was observed to be a host cell for vesicular stomatitis virus, showing cytopathic effect upon infection. In the cells PorLeIF and PorIIF did not exhibit any antiviral activity, but reference human α - and β -interferons exhibited the cross-species antiviral activity, indicating that BGK cell line can be used for human interferon assay.

Fluorescamine을 이용한 sulfonamide의 형광 분석법

임재영,한수남,이문한,박종명,Lim, Jae-young,Han, Su-nam,Lee, Mun-han,Park, Jong-myung 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.1

These experiments were carried out to develop a novel, simple, and rapid method to determine urinary sulfonamides using fluorescamine by spectrofluorometry. To get optimal conditions for the sulfonamide-fluorescamine reaction, sulfonamides such as sulfamethazine, sulfamerazine, sulfadimethoxine and sulfamonomethoxine, dissolved in buffers with various pH ranges were reacted with various concentrations of fluorescamine. and then, the fluorescence intensity and stability of the fluorophore were measured. To eliminate the interfering substances in urine, the fluorophore in buffers and urine with a definite pH range was extracted with some organic solvents. After then the fluorescence intensity was measured in organic and aquous phases. The results obtained were summarized as follows: 1. The maximal fluorescence of sulfonamides was presented in acidic state, pH 4.5~5.0, at 30 minutes after reaction. 2. The optimal concentration ratio of sulfamethazine and ffuorescamine was more than 1 : 40 in mole. 3. In pH 4.0, the intensity was maximal but was time-dependent, whereas in pH 8.0, the intensity was time-independent. 4. Sulfamethazine-fluorescamine conjugate could be dissolved in some of organic solvents in acidic state such as chloroform, n-butanol, and ethylacetate. 5. Sulfamethazine-flnorescamine conjugate in swine urine coule be extracted with ethylacetate in acidic state, pH 4.0~5.0.

B형간염(型肝炎) 표면항원(表面抗原)의 주면역원(主免疫原) 결정기(決定基)에 특이(特異)한 합성(合成) Peptide의 면역원성(免疫原性)에 관한 연구(硏究)

신광순,한수남,Shin, Kwang-soon,Han, Su-nam 대한수의학회 1985 大韓獸醫學會誌 Vol.25 No.1

Many investigators have been pursuing various attempts so far to produce hepatitis B surface antigen(HBsAg) vaccines using the techniques such as isolation from plasma of chronic HBsAg carrier, recombinant DNA technique or preparation of synthetic peptides specific for immunogenic determinants. Hepatitis B virus can not grow on any cell lines by the tissue culture technique at the present time. The plasma of chronic HBsAg carrier is expensive and its source is limited. The HBsAg from the recombinant DNA technique gave still very low yield. Another approach, therefore, has been initiated to develop a synthetic hepatitis B virus vaccine. The possible use of several distinct synthetic vaccines in prophylaxis can be facilitated by availability of full synthetic immunogens. Peptides synthesized for potential application as antiviral vaccines have been mostly tested in the form of conjugates with carrier proteins, although the free synthetic peptide can be immunogenic. To understand basic knowledges on the antigenicity and immunogenicity of a synthetic peptide specific for major immunogenic determinant of HBsAg, a nonapeptide, $H_2N^{139}Cys-Thr-Lys-Pro-Thr-Asp-Gly-^{146}Asn-Aba$ COOH, which corresponds to HBsAg amino acid residues 139 to 147, was synthesized by the Merrifield's solid-phase method with a slight modification. The antigenicity and immunogenicity of this specific synthetic peptide were examined comparing with purified plasma-derived natural HBsAg. The results obtained are as follows; 1. The peptide synthesized showed the identical amino acid composition to the theoretical value. The degree of purification and molecular weight were acertained by methods of high performance liquid chromatography and mass spectrometry. 2. Using m-maleimidobenzoyl-N-hydroxysuccinimide ester as a conjugating agent, the synthetic peptide was conjugated to rabbit albumin and ${\gamma}$-globulin, tetanus and diphtheria toxoids, and keyhole limpet hemocyanin. Their conjugation yields were 8.3, 9.5, 15.8, 13.5, and 11.2%, respectively. 3. The natural HBsAg was purified from plasma of chronic HBsAg carrier. By the electron microscopic observation of the purified natural HBsAg preparation, no Dane particles were observed and the preparation showed negative DNA polymerase activity. 4. Antigenicity of the synthetic peptide and the plasma-derived natural HBsAg was determined by competition radioimmunoassay using $^{125}I$-natural HBsAg. Their 50% inhibitions appeared as $90{\mu}g/ml$ and $0.12{\mu}g/ml$ for the synthetic peptide and the natural HBsAg, respectively. This indicates that the former was about 750-fold less antigenic than the latter. 5. Immunogenicity of the synthetic peptide was determined by administering the peptide-carrier conjugates into rabbits with and without Freund's complete adjuvant. Regardless the carrier proteins and adjuvant, positive immune responses to the synthetic peptide were observed. The higher antibody titers, however, were shown in the groups administered with Freund's complete adjuvant. 6. Immunizing dose 50% in mice of the various peptide-carrier conjugates was 5.47, 6.00, 65.16, 31.25 and $13.03{\mu}g/dose$ for rabbit albumin and ${\gamma}$-globulin, tetanus and diphtheria toxoids, and keyhole limpet hemocyanin, respectively, while the natural HBsAg showed $0.65{\mu}g/dose$. 7. It was postulated that homologous proteins prefer to heterologous ones as the carriers.

Thin Layer Chromatography 및 Automatic Amino Acid Analyzer에 의한 소, 돼지, 산양(山羊) 및 토끼 고기 중의 유리(遊離)아미노산 분포(分布)

조종후,조태행,한수남,Cho, Jong Hoo,Cho, T.H.,Han, Su Nam 대한수의학회 1973 大韓獸醫學會誌 Vol.13 No.1

Free amino acid extracts of musculus brachiocephalicus and musculus glutaeus of cattle, swine, goats and rabbits are analyzed to see the composition and the contents of free amino acids by thin layer chromatography and automatic amino acid analyzer. The results obtained are summarized as follows: 1. Meats of cattle, swine, goats and rabbits analyzed by thin layer chromatography have 6 kinds of free amino acids such as glycine, histidine, methionine, lysine, alanine and leucine, and the spots of glycine in chromatogram of meats of cattle, swine and goats and the ones of methionine in chromatogram of meats of rabbits are the largest and the most denes of all other ones. 2. Distribution curves of free amino acids in meats of cattle, swine, goats and rabbits showed significant differences, but differences on distribution curves of free amino acids between musculus-brachiocephalicus and musculus glutaeus of the same tested animal are not significant. 3. Contents of free amino acids in meats of cattle, swine, goats and rabbits showed significant differences, but differences on contents of free amino acids between musculus brachiocephalicus and musculus glutaeus of the same tested animal are not significant.

계란중 요드의 간편한 비색 측정법

Jong Myung Park(朴鍾鳴),Su Nam Han(韓壽南),Mun Han Lee(李文漢) 한국예방수의학회 1981 예방수의학회지 Vol.5 No.1

A simple and relatively accurate method to determine iodine in iodine-enriched eggs was developed by modifying spectrophotometry and summerized as following: Egg yolk and egg white were digested by ethanol and potassium hydroxide on the steam bath, and free and protein-bound iodine were converted to potassium iodide by these procedures. The samples were dried, charred and then ashfied at 500℃ for 4 hours. Potassium idodide in ash residue was extracted and washed with distilled water. The potassium iodide in extract was treated with 6N sulfuric acid and 3% hydrogen peroxide solution to liberate free iodine. The free iodine was extracted with chloroform and the absorbance of iodine in chloroform was determined at the wave length of 510nm with chloroform blank. Although carbon tetrachloride and carton disulfide were found being better solvents for iodine in intensity of absorbance and in efficiency of extracting iodine, the authors recommend chloroform as an iodine solvent, since chloroform was less toxic and more benefit to handle and the iodine content in iodine-enriched eggs is high enough to determine with chloroform. The iodine contents in normal eggs, iodine-enriched eggs in Korea and in Japan and in the sea weeds were reported. Distribution of iodine in egg albumin and egg yolk was also described. The authors recommend this method as an assay method of iodine in livestock products.