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        Plants cultivated in Choco, Colombia, as source of repellents against Tribolium castaneum (Herbst)

        Karina Caballero-Gallardo,Nayive Pino-Benitez,Nerlis Pajaro-Castro,Elena Stashenko,Jesus Olivero-Verbel 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4

        Essential oils (EOs) of eight plants collected in Choco, Colombia, including Piper divaricatum, P. pseudolanceifolium,P. confertinodum, P. diazanum, Ocimum campechianum, Siparuna conica, Mikania micrantha and Hedychiumcoronarium, were analyzed by gas chromatography–mass spectrometry and tested as repellents against Triboliumcastaneum, using the area preference method, after 2 and 4 h exposure. The main components found in EOswere methyl eugenol, trans-β-cariophyllene, methyl eugenol, α-pinene, δ-cadinene, γ-elemene, α-pinene and1,8-cineol, for O. campechianum, P. pseudolanceifolium, P. divaricatum, P. confertinodum, P. diazanum, S. conica,M. micrantha, and H. coronarium, respectively. Best repellent activities were observed for oils fromO. campechianum and P. pseudolanceifolium with mean repellent concentration (RC50) values of 0.00006 and0.0001 μL/cm2 after 2 h, and 0.00003 and 0.0001 μL/cm2 after 4 h, respectively; whereas the least potent wasthat from M. micrantha, with RC50 values of 0.074 and 0.040 μL/cm2 at 2 and 4 h exposure times, respectively. Based on average percentage repellence, oils from P. pseudolanceifolium and O. campechianum were classifiedas Class IV repellents and were better than the commercial repellent IR3535, classified as Class II. These data evidencethe Choco region as an important source of natural repellents with promising commercial opportunities.

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