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Suresh Sripada,D. Esther Kalpana Rani,G. Upender,P. Gayathri Pavani 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.5
Titanium boro tellurite glasses in the xB<sub>2</sub>O<sub>3</sub>−(90−x) TeO<sub>2</sub> + 10TiO<sub>2</sub> (where x = 0 to 50 mol%) system were prepared by using the conventional melt-quenching technique. Glass transition temperatures were measured with differential scanning calorimetry (DSC) and found to be in the range of 300 - 370 ℃. The Raman spectra showed a cleavage of the continuous TeO<sub>4</sub> (tbp) network by breaking of the Te-O-Te linkages. The relative transition of TeO<sub>4</sub> − groups to TeO<sub>3</sub> − groups is accompanied by a change in the oxygen coordination of the boron from 3 to 4 (BO<sub>3</sub> <sup>−</sup> to BO<sub>4</sub> <sup>−</sup>). The impedance plots Z▼(┺) versus Z▽(┺) for all the glass samples were recorded and found to exhibit a single circle. The AC conductivity of all glass samples was studied in the frequency range from 100 Hz to 1 MHz and in the temperature range from room temperature (RT) to 375 ℃. The AC conductivity decreased by about one order in magnitude with increasing B<sub>2</sub>O<sub>3</sub> content. The conductivity was found to be on the order of 10<sup>−4.5</sup> to 10<sup>−6</sup> (┯-cm)<sup>−1</sup> at 375 → and 1 MHz for 10 mol% and 50 mol% B<sup>2</sup>O<sup>3</sup> contents, respectively. The relaxation behavior in these glass samples is discussed based on the complex modulus and impedance data.
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Khadija Ahansal,Hanane Aadel,Sripada Mahabala Udupa,Fatima Gaboun,Rabha Abdelwahd,Mohammed Ibriz,Driss Iraqi 한국식물생명공학회 2022 JOURNAL OF PLANT BIOTECHNOLOGY Vol.49 No.2
An efficient genetic transformation protocol is a fundamental requirement for high regeneration capacity from cultivated durum wheat (Triticum durum) varieties. In this study, wereportedtheeffectsoftwoauxins,2,4-dichlorophenoxyaceticacid(2,4-D)and4-amino-3,5,6-trichloropicoli nicacid(picloram), at a concentration of 2 mg/Laloneandincombination on the embryogenic callus and plantlet regeneration of four durum wheat varieties (Amria, Chaoui, Marouane, and Tomouh) using mature embryos (MEs) and immature embryos (ImEs). Significanteffectsofvariety,culturemedium(theauxinused),andvariety-mediuminteraction were observed on the callus weight and plantlet regeneration of both MR and ImE explants. The medium used for callus induction significantly affected plantlet regeneration (p < 0.001). Comparedto2,4-D, picloram led to a higher plantlet regeneration rate in both ME and ImE explants (19.8% and 40.86%, respectively). Plantlet regeneration also varied significantly depending on the variety and medium used. PicloramledtohighplantletregenerationofbothME and ImE explants in all varieties except Tomouh, which showed high plantlet regeneration of ME explants in 2,4-D. A comparison of ME and ImE responses indicated that ImEs are the best explants for high plantlet regeneration in durum wheat. Ourfindingssuggestthatpicloramisthebestauxin and should be used instead of 2,4-D due to its positive effect on increasing plant regeneration of durum wheat ME and ImE explants.
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Biolistic transformation of Moroccan durum wheat varieties by using mature embryo-derived calli
Chaimae Senhaji,Fatima Gaboun,Rabha Abdelwahd,Ghizlane Diria,Sripada Udupa,Allal Douira,Driss Iraqi 한국식물생명공학회 2021 JOURNAL OF PLANT BIOTECHNOLOGY Vol.48 No.4
Environmental stresses are estimated to have reduced global crop yields of wheat by 5.5%. However, traditional approaches for the transfer of resistance to these stresses in wheat plants have yielded limited results. In this regard, genetic transformation has undoubtedly opened up new avenues to overcome crop losses due to various abiotic stresses. Particle bombardment has been successfully em- ployed for obtaining transgenic wheat. However, most of these procedures employ immature embryos, which are not available throughout the year. Therefore, the present investi- gation utilized mature seeds as the starting material and used the calli raised from three Moroccan durum wheat varieties as the target tissue for genetic transformation by the biolistic approach. The pANIC-5E plasmid containing the SINA gene for drought and salinity tolerance was used for genetic trans- formation. To enhance the regeneration capacity and trans- formation efficiency of the tested genotypes, the study compared the effect of copper supplementation in the induc- tion medium (up to 5 μM) with the standard MS medium. The results show that the genotypes displayed different sensitivities to CuSO4, indicating that the transformation efficiency was highly genotype-dependent. The integration of transgenes in the T0 transformants was demonstrated by polymerase chain reaction (PCR) analysis of the obtained resistant plantlets with primers specific to the SINA gene. Among the three genotypes studied, ‘Isly’ showed the highest efficiency of 9.75%, followed by ‘Amria’ with 1.25% and ‘Chaoui’ with 1%.
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Pradyumna Raval,Alistair I.W. Mayne,Phey Ming Yeap,Thomas Barry Oliver,Arpit Jariwala,Sankar Sripada 대한고관절학회 2019 Hip and Pelvis Vol.31 No.1
Purpose: Occult hip fractures in the elderly can be missed on standard radiographs and are a known cause of morbidity. These are generally diagnosed on either magnetic resonance imaging (MRI) or computed tomography scan, depending upon local hospital policy. While there is an abundance of literature on hip fractures in general, little is known about the clinical outcome of patients with occult hip fractures. The aim of this study was to review the demographics, injury characteristics, management and clinical outcome of patients diagnosed with occult femoral neck fractures on MRI. Materials and Methods: Using an existing hospital database, a retrospective analysis of all patients with occult hip fractures diagnosed by MRI scan from 2005 to 2014 was conducted. Results: Sixty-four patients (23 males and 41 females) were included. The mean duration of hospitalisation was 16 days. A significantly higher percentage of patients were discharged to their pre-existing residence compared to National Institute for Health and Care Excellence (NICE) commissioning guidelines (66% vs. 45%). The 30-and 60-day mortalities were 3% and 10%, respectively. Mortality was lower in patients who underwent internal fixation (n=3/31) compared with those undergoing replacement (hemi/total hip arthroplasty) (n=5/12) (P=0.056). Conclusion: Patients with occult hip fractures diagnosed on an MRI scan are more likely to be discharged to their pre-existing residence and have lower mortality rates compared to NICE guidelines and National Hip Fracture Database (NHFD).
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