An S-locus receptor-like kinase in plasma membrane interacts with calmodulin in <i>Arabidopsis</i>

Kim, Ho Soo,Jung, Mi Soon,Lee, Kyunghee,Kim, Kyung Eun,Yoo, Jae Hyuk,Kim, Min Chul,Kim, Doh Hoon,Cho, Moo Je,Chung, Woo Sik Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>Calmodulin-regulated protein phosphorylation plays a pivotal role in amplifying and diversifying the action of calcium ion. In this study, we identified a calmodulin-binding receptor-like protein kinase (CBRLK1) that was classified into an S-locus RLK family. The plasma membrane localization was determined by the localization of CBRLK1 tagged with a green fluorescence protein. Calmodulin bound specifically to a Ca<SUP>2+</SUP>-dependent calmodulin binding domain in the C-terminus of CBRLK1. The bacterially expressed CBRLK1 kinase domain could autophosphorylate and phosphorylates general kinase substrates, such as myelin basic proteins. The autophosphorylation sites of CBRLK1 were identified by mass spectrometric analysis of phosphopeptides.</P><P><B>Structured summary</B></P><P>MINT-6800947:<I>CBRLK1</I> (uniprotkb:Q9ZT06) and <I>AtCaM2</I> (uniprotkb:P25069) <I>bind</I> (MI:0407) by <I>electrophoretic mobility shift assay</I> (MI:0413)</P><P>MINT-6800966:<I>AtCaM2</I> (uniprotkb:P25069) and <I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>bind</I> (MI:0407) by <I>competition binding</I> (MI:0405)</P><P>MINT-6800930:<I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>binds</I> (MI:0407) to <I>AtCaM2</I> (uniprotkb:P25069) by <I>far Western blotting</I> (MI:0047)</P><P>MINT-6800978:<I>AtCaM2</I> (uniprotkb:P25069) <I>physically interacts</I> (MI:0218) with <I>CBRLK1</I> (uniprotkb:Q9ZT06) by <I>cytoplasmic complementation assay</I> (MI:0228)</P>

초·중·고등학교 학생·학부모·일반교사의 보건교육 영역별 요구도와 보건교육

윤순녕,김영임,최정명,조희순,김영희,박영남,오경순,이분옥,조선녀,조소영,한선희,하영미 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: The purpose of this study was to identify the needs of health education in students, their parents and teachers in the elementary, middle and high schools and the current situation of health education class. Method: The subjects of this study were a total of 9450 persons including students, their parents and teachers from 279 schools throughout the country. They were selected through convenient sampling. Data were analyzed through x^(2)­test and ANOVA. Result: Students, their parents and teachers replied that 18 dimensions of health education class (DHEC) are necessary. The four DHEC - healthier life style, sex education, mental health and safety education - showed high educational needs in students, their parents, and teacher. High school students had higher educational need of 'symptom management for daily living' than elementary and middle school students. Students, their parents and teachers in elementary school had higher educational needs of 17 DHEC that those in middle and high school. The percentages of schools with health education class taught by health teachers were 99.2%, 75.5% and 66.0% respectively in elementary, middle and high schools. Health education was given mainly using physical education classes at elementary schools, and creative class hours at middle and high schools. In general, health education took 1-3 hours per week at elementary schools, and less than an hour at middle and high schools. Conclusion: Therefore, based on the results, systematic health education class should begin from elementary school to meet the need of health education in students, their parents and teachers, and further study should be made on the number of hours required and the amount of contents of 18 DHEC.

일반논문 : 고통의 시대와 저항담론으로서의 불교사상 -1970년대 이후 한국문학에서의 불교사상 수용 양상과 그 의미-

한순미 ( Soon Mi Han ) 전남대학교 호남학연구원 2012 호남학 Vol.0 No.51

이 글은 70년대 이후 한국문학 작가들이 고통의 시대현실에 대응하기 위한 저항담론으로서 불교사상을 어떠한 맥락에서 수용하였는지를 읽고자 했다. 작가들은 불교사상에서 암울한 시대 상황에 맞서는 민중사상의 바탕을 마련하고 이를 미학적인 실천의 원리로 수용함으로써 민중문학의 저항담론을 견고하게 다듬어 나갔다. 미륵사상은 70~80년대를 가로지르는 한국문학의 대표적인 저항담론이라고 해도 지나치지 않다. 작가 송기숙, 고은, 김지하에게 미륵사상은 민중주체에 의한 현실사회의 개혁을 이루어 새로운 미륵세상, 즉 이상사회를 구축하는 사상적 힘이었다. 이어서 작가 한승원, 송기숙, 윤흥길, 문순태의 소설에서 미륵설화와 미륵신앙이 수용된 양상을 살펴보았다. 이들의 소설에서 미륵은 역사적 고통이 지속되고 있는 현실을 드러내는 매개적 장치이다. 미륵은 언젠가 새로운 세상이 올 것이라는 희망의 징표이기도 하지만 그것은 아직 실현되지 않은 비극의 징표이기도 한 것이다. 조정래의 소설 『대장경』은 고려대장경이 무신정권의 정치적 이용물의 하나로 추진되었다는 견해를 제시한다. 수기대사의 비판적 시선을 통해서 유신독재 하에서 지식인 작가가 지켜내야 할 저항성을 보여준다. 이러한 점은 정치권력에 대항하여 사실을 기록하려는 화가 솔거의 저항적인 예술가의 형상으로 이어진다. 마지막으로 고은의 『화엄경』에서 제출하고 있는 주요한 물음을 민족문학 작가들이 현실문제에 대해 그 해결방안을 모색하였던 작업과 관련하여 해석하였다. 그 대안으로 화엄사상과 대승불교의 보살사상, 즉 평등과 사랑의 실천을 제기하고 있음을 읽을 수 있었다. 70년대 이후 한국문학 작가들은 불교사상의 전통을 재해석하면서 문학적 저항담론을 마련해 왔다. 그 가운데 작가들이 호남의 미륵사상에 특별한 관심을 둔 것은 호남의 불교사상이 지닌 오랜 저항적 흐름과 의향 호남의 문화표상이 한국문학 속에 자리한 흔적을 보여준다. 여기에서 호남 불교사상과 현대문학이 공유한 정신사적 궤적과 그 깊이를 헤아릴 수 있었다. 아울러 저항담론으로서의 불교사상은 자본주의적 근대화를 극복하고 대안적 근대성을 마련하기 위한 노력이었다. 따라서 한국 현대문학에서 불교사상이 어떻게 수용, 지속되어 왔는지를 살피는 연구는 지역문학과 한국문학의 사상적 변화를 가늠할 수 있음은 물론이고 지금 한국 사회가 처한 공동체, 다문화주의 등 현실적인 문제점을 해결하는 연구에도 보탬이 될 수 있을 것이다. This study is to read how and in what contexts the writers of Korean literature have accepted Buddhist thought as resistance discourses to cope with the age of suffering since 1970`s. The writers established the basis of people`s thought from Buddhist thought against the dark situation at that time, and accepted that as the principle of aesthetical practice to solidify resistance discourses of people`s literature. Maiterya thought can be told enough to be a representative resistance discourse of Korean literature over 1970`s through 1980`s. For writers such as Song Ki-suk, Go Eun, and Kim Ji-ha, Maiterya thoughts was ideological power to establish an ideal society, that is, new Maiterya world through social revolution by the people. And then, the study researched the acceptance patterns of Maiterya tales and beliefs shown in the novels written by Han Seung-won, Song Ki-suk, Yoon Hong-gil, and Mun Soon-tae. Maiterya in their novels is a mediate device revealing the reality of historical suffering. Maiterya is a hopeful sign that will bring a new world someday, but it also a sign of tragedy not realized yet. Jo Jung-rae`s novel『Daejangkyeong』(meaning the complete collection of Buddhist Sutra in Korean) suggests that Koryeo Daejangkyeong was promoted as a political use by the Military Rule. Through Suki Daesa`s critical point of view, it shows the resistance that intellectual writers should keep under Yushin Dictatorship. This is connected to artist Solgeo who painted the fact in his pictures against the political power. Lastly this study interpreted major questions in Ko Un`s『Little Pilgrim』(Hwaeumkyeong in Korean) in connection with the work that writers of national literature tried to find a solution of practical problems. The alternative was Hwaeum Thought and the Bodhisativas` thought of the Mahayana, that is, the practice of equality and love. Since 1970`s, the writers of Korean literature have showed literary resistance discourses, re-interpreting the tradition of Buddhist thought. Especially, the writers had interest in Maiterya thought of Honam area, which is the sign of long resistant flow that Buddhist thought has and cultural representation of Honam as the hometown of justice in Korean literature. At the point, we can understand the trace and depth of spiritual history that Honam`s Buddhist thought and modern literature share between them. In addition, Buddhist thought as resistance discourses was an effort to overcome capitalistic modernization and to find an alternative modernity. In conclusion, the study to research how Buddhist thought was accepted and continued in Korean modern literature is not only to understand the change in thoughts of local and Korean literature, but also to be helpful for the researches to find a solution of practical matters Korean society currently has such as the community and multiculturalism.

서울지역 여대생들의 떡에 대한 인식 및 섭취 실태 조사

김영순,오순덕,김향미,신지홍,김현경,배유미,조규영,조성환 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.1

This study conducted a survey of the improvement and popularization for Korea Rice Cakes, a Korean traditional food, on two hundred women students in five colleges in Seoul, Korea. It was investigated to get the information of the recognition, preference, and consumption patterns for the succession and improvement of Korean Rice Cakes. The results are as follows: 1. A portion of 41.5% among responders knows from three to six kinds of Korean Rice Cakes, and of 28% can make at least one among the kinds of Korean Rice Cakes. 2. A portion of 91% recognizes that Korean Rice Cakes are one of well-nourished food, but of 42% answers that Korean Rice Cakes cannot be replaced with the principle food. Also, there are no significant differences in the recognition of Korean Rice Cakes as the kinds of religions. 3. The frequency of purchase is about one or twice in a month (35.5%). Consumers (36.5%) intend to purchase Korean Rice Cakes for the commemoration of national holidays or anniversaries. 4. The reasons for the preference of other foods more than Korean Rice Cakes are the easiness of purchase (36.0%), the deliciousness (21.5%), the diverse kindness (17.5%), and the proper package (9.5%). It is important that constant and particular school / home education is need to improve and success Korean Rice Cakes.

자궁적출술을 받은 부인의 인공폐경 증상 관련 요인

장순복,최연순,박소미,전은미 최신의학사 1990 最新醫學 Vol.33 No.10

초·중·고등학교 학생, 학부모, 일반교사의 보건교육 요구

윤순녕,김영임,최정명,조희순,김영희,박영남,오경순,이분옥,조선녀,조소영,한선희,하영미 韓國學校保健學會 2004 韓國學校保健學會誌 Vol.17 No.2

Purpose : The purpose of this study was to identify needs of health education of the students, parents, general teachers, and health teacher of the elementary, middle, and high schools. Method : 279 schools of elementary, middle, and high school were sampled nonproportionally during the period from June 28 to July 26, 2004 . The data were collected by the questionnaire from 1,888 students, 1,876 parents, 1,695 general teachers, 279 health teachers. A frequency, % and χ2-test were used to analysis by SAS program. Result : 1. It showed that 98.1% of elementary school students, 92.3% of middle school students, 89.6% of high school students answered that they need health education. The most of students, parents, and general teachers had high interests in health education. 2. In the case of students, main causes of needs of health education was 'to prevent diseases or accidents''. But parents answered that it was 'to build up a healthier behavior'. General teachers answered that it was 'more effective systematic and continuous health education'. 3. Over 80% of students, parents, and general teachers about the question of who qualified person is to teach health education as a regular class responded that health teacher is available. Especially 93% of elementary school students answered like that. 4. The most of students, parents, and general teachers answered that health education in the classroom is favorable 1 to 2 hours per week. Conclusion : As results of the study , the majority of students, parents, general teachers agreed needs of health education. Therefore, on the basis of this study, systematic and continuous health education is necessary. Additionally the establishment of independent health subject is required certainly.

동서양의 식용꽃에 대한 고찰 : 조리방법을 중심으로

김영순,이정희,오순덕,정은미,최지영,유은순,장정미,정진선,하상민 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.2

Edible flower means a flower that is good to eat is subject to either cooking or appetizing. In general, people eat edible flower as raw so that the loss of vitamin and inorganic are minimal. Edible flower is usually utilized as salad material or aromatic, decoration for jelly or cake and solid stuff in soup. Thus, decorating food by edible flower improves flavor, fragrance and appetite. Edible flower serves various purposes both east and west. In Korea, Edible flower is mainly subject to soup, cooked potherbs, frying, tea stuff, brewing and etc, whereas in Japan, its various usage covers cooked potherbs, pickle, sliced raw fish, frying, salad, jelly and decoration. On the other hand, Edible flower is used as numerous salad and tea stuff, condiment, roasting, meat, sweets and desserts in Western Hemisphere.

위절제술 환자의 표준진료지침 개발 및 적용 효과

김은희,김철규,이순교,김순덕,이혜옥,권정순,이경미,이민미,심순미,유용만,신종식,강은희,이상일,김병식,오성태,육정환,박수길 한국의료QA학회 2003 한국의료질향상학회지 Vol.10 No.2

Background : Gastric cancer is the most common malignant tumor in Korea. surgical operation is one of the major treatment modalities for gastric cancer patients. Therefore, gastrectomy is one of the most common procedures in General Surgery. There were variation in length of hospital stay and medical treatment for gastrectomy between three surgeons at Asan Medical Center. Clinical pathways have received considerable attention as a tool for recucing the medical practice variation, increasing the efficiency of care process, and improving the quality of care. The aim of this study was to evaluate the effect of a clinical pathway for gastrectomy in gastric cancer patients. Methods : The clinical pathway for gastrectomy was developed and implemented by a multidisciplinary group in Asan Medical Center. A computerized clinical pathway program was developed and revised after a pilot test. A total of 145 patients underwent gastrectomy by three surgeons at Asan Medical Center. We compared the length of hospital stay, patient satisfaction, and unplanned readmission rate between the pre-pathway group(n=67) and the post-pathway group(n=78). We also investigated the degree of satisfaction among the physicians and nurses who were main end-users of the clinical pathway. Results : The clinical pathway was applied to all target patients. The average length of hospital stay was shortened from 12.7days to 10.6days(p<0.01). The degree of patient satisfaction with the care process changed from 90.3% to 89.2% after the implementation of the clinical pathway, but the difference was of satistically significant(p=0.761). Unplanned readmission rate was 2.9% in the pre-pathway group. More than 90% of physicians and nurses answered that the clinical pathway had been a useful tool in their medical practice. Conclusions : The findings of the study demonstrated that implementation of the clinical pathway for gastrectomy produced substantial reduction in the length of hospital stay while improving the quality of patient outcomes. The computerized clinical pathway program can be used as one of the powerful patient management tools for reducing the practice variations and increasing the efficiency of care process in Korea hospital settings.

신규간호사 배치후 교육시 프리셉터쉽 적용 사례연구

김창희,김혜숙,조혜성,이순옥,함형미,박미미 성인간호학회 2000 성인간호학회지 Vol.12 No.4

The purpose of this study was to examine the effect of preceptorship on On the Job Training of new nurses. The research design was a retrospective survey and one group pre-rost test design. The subjects were 49 entry nurses and 49 preceptors The data were collected from Feburary 1, 1998 to December 1, 1998. One questionnair applied in this study consisted of general characteristics, the responses of new entry nurses and their preceptors and their qualities. The other questionnair consisted of job satisfaction of new entry nurses and Preceptors and their competencies. The data were analyzed for frequencies, mean score, standard deviation, t-test, and ANOVA. The results of the study were as follows : 1) Preceptees's responses were that the nurses' affiliation of unit. cooperation of other nurses, and competencies of nursing skills improved after OJT applied preceptorship. 2) Preceptors's answers were that preceptorship had a positive effect on their continuing education. 3) While preceptees's job satisfaction scores were high in interaction, administration, and organization, they were low in autonomy and pay. 4) Pre-competency scores were the same as post-competency scores. 5) After preceptorship, preceptors responded that their nursing skill & communication competency increased, but their nursing administration competency decreased. 6) Finally, preceptorship's other effects were as follows: ① Checklists for preceptee education at the nursing unit were developed separately. ② It was proved that 'preceptor interview note' was effective on manage preceptorship. ③ We obtained duty orierlap periods, medical-surgical units-1 month. 5 ICU-2 months. operaction room-3 months ④ We prepared a preceptor program based on the study results.

연령증가에 따른 혈압요소들의 좌심실비대 및 관동맥질환과의 관련성

박재석,박창규,박미영,박재형,김용현,나진오,신성희,서순용,홍순준,박성미,임홍의,김응주,서홍석,오동주,노영무 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2