Effects of Intravenous Adminitration of Nimodipine on Cerbal Pial Vessels after Experimental Subarachnoid Hemorrhage in Rabbits

Hwang, Soon Gu,Lee, Young Woo 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.7

가토에서 대조내 자가 신선혈 0.2㎖를 주입 후 6시간에 생리식염수와 여러 농도의 운nimodipine 용액(10, 30 및 60㎍/㎏)을 각각 정맥주사하여 야기되는 뇌연막혈관들의 반응(직경변화)을 연구하였다. 혈관의 측정은 개두술 후 수술현미경 최고 확대(40×)하에서 현미경의 대안렌즈에 Filar micrometer eyepiece(424 and 426, AO)을 부착하여 시행하였다. 이 Filar micrometer eyepiece의 한 눈금은 3.4㎛로 매우 미세한 소혈관까지 직접 측정할 수 있었다. PaO₂, PaCO₂ 및 동맥혈 pH는 대개 전 실험을 통해서 의의있는 변화를 보이지 않았다. 수축기 동맥압은 nimodipine 용액 주입 직후에 다소의 하강을 보였으나(p<0.05) 이러한 변화는 수축기 동맥압의 일시적인 하강현상이고 곧 정상 수준으로 복귀했다. 실험적 뇌지주막하 출혈 6시간 후 nimodipine 용액과 동일한 량의 생리식염수를 주입하였을 때 뇌연막혈관의 반응은 미미하여 그 통계학적 의의는 없었다(p>0.05). 그러나 nimodipine 용액을 정주하였을 때는 Group Ⅰ, Group Ⅱ 그리고 Group Ⅲ 모두에서 뇌연막혈관의 확장을 보였다. 이러한 혈관의 확장은 동맥, 특히 소동맥에서 현저한 양상을 보였으며 대연막동맥에서도 상당히 의의있는 혈관 확장을 보이기는 했으나 소연막동맥에서만큼 현저하지는 않았다. 이러한 뇌 연막동맥의 확장은 약물 주입 후 5, 10분에 가장 뚜렷하였으며 그 후는 시간이 경과함에따라 혈관의 직경이 서서히 감소하는 양상을 보였다. 뇌연막정맥의 경우에도 nimodipine용액의 정주 후 미약한 정도의 혈관확장을 보였으나 그 확장된 정도는 뇌연막동맥에 비해 미미하였으며 통계학적 의의도 적거나 없었다. 뇌연막동맥의 nimodipine에 대한 반응은 10, 30 그리고 60㎍/㎏의 약물 양의 변화에 비교적 민감하여 용량에 비례하는 소견을 보였다. 이상의 결과에서 뇌 지주막하 출혈이 야기된 실험동물에 nimodipine을 정주했을 때 뇌연막동맥의 현저한 확장이 야기되며 특히 소동맥이 민감하게 확장되는 사실은 뇌지주막하출혈에 의한 뇌허혈성 질환에 있어 nimodipine의 치료적 유용성을 입증할 수 있는 좋은 증거가 될 것으로 생각되며 그 효능은 정주된 실험약물의 양에 비례하였다. 이로서 부작용이 허용되는 범위 내에서 다량의 nimodipine을 허혈성 뇌혈관질환에 사용하여 치료적인 효과를 기대할 수 있을 것이라 생각된다. The author have studied effects of intravenous injection of normal saline and nimodipine solution(10, 30 and 60㎍/㎏) on cerebral pial vessels at 6 hours after experimental subarachnoid hemorrhage. Measurement of pial vessels were performed under operation microscope through the bilateral craniectomy area. Filar micrometer eyepiece was attached on the oculus of microscope(40×) to make it easier to measure fine pial vessels directly. Measuring unit of this micrometer eyepiece was 3.4㎛. Physilogic parameters(PaO₂, blood pH) generally did not show significant change all through the experiments except that systolic blood pressure showed temporary decrease immediately after the injection of nimodipine solution, which returned to base level soon. Small pial arteries were by far most effectively dilated by intravenous injection of nimodipine solution especially in 5 minutes. Large arteries also presented significant dilatation after the injection. The arteries were dilated as dose-dependent manner. Veins showed dilatation to the lesser degree than arteries independent of dosage of nimodipine, that was statistically of no significance. Conclusively, nimodipine might be used in treatment of vasospasm in subarachnoid hemorrhage in practice.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

PDP용 유전체의 반사특성과 유전특성

권순석,김명녕,이상래,황문구 한밭대학교 2004 한밭대학교 논문집 Vol.21 No.-

이 논문에서는 P₂O_(5)-ZnO-BaO계와 SiO₂-ZnO-B₂O₃계의 반사특성과 유전특성이 연구 되었다. P₂O_(5)-ZnO-BaO계의 반사율은 SiO₂-ZnO- B₂O₃계의 반사율보다 낮게 나타났고, TiO₂의 함유량이 증가할수록 반사율이 감소되었다. 또한 P₂O_(5)-ZnO-BaO계의 유전율은 SiO₂-ZnO-B₂O₃계의 유전율보다 높은 값을 보였고, TiO₂ 함유량이 증가함에 따라 두 계 모두 유전율이 증가되었다. 이 현상은 공간전하효과로 설명될 수 있다. 본 연구의 결과는 높은 반사율과 높은 절연파괴 강도가 요구되는 플라즈마 디스플레이(PDP)의 하판 유전체, 또는 플라즈마 현상을 응용하는 조명 및 광고용 기구의 반사 유전체로서 응용이 가능할 것으로 기대된다. In this paper, the reflectance and the dielectric characteristics for P₂O_(5)-ZnO-BaO system and SiO₂-ZnO-B₂O₃ system have been investigated as a function of contents of TiO₂. The reflectance was decreased with increasing the contents of TiO₂ contents, and the reflectance of P₂O_(5)-ZnO-BaO system was lower than that of SiO₂-ZnO-B₂O₃ system. The dielectric constant of P₂O_(5)-ZnO-BaO system was higher than SiO₂-ZnO-B₂O₃ system, and the dielectric constant in the both system was increased with increasing of TiO₂ contents. This could be explained as the space charge effects. These results are could be applied to the under plate dielectrics of PDP required high reflective ratio and breakdown strength.

전하 제어법을 이용한 압전 액추에이터의 이력저감

정순종,이대수,송재성,홍원표,강은구,최원종 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

This paper presents a method to reduce hysteresis in multilayer ceramic actuator by connecting the actuator with a capacitor in a series circuit. The change in hysteresis with respect to the capacitor was examined. 0.2Pb(Mg_(1/3)Nb_(2/3))O₃-0.8Pb(Zr_(0.475)Ti_(0.525)) O₃ ceramic material was used as a piezoelectric material for the actuator. Displacement of the actuator was measured in a capacitive gap sensor measuring system. In case of inserting a capacitor in a total circuit, hysteresis became dramatically decreased, and then finally the hysteresis value can be reduced below 0.2%. It was found in this present study that reducing the hysteresis in the actuator is dependent upon the characteristics of the capacitor in total circuit and also operating frequency

고탁도시 DAF 정수장의 운영 및 진단

권순범,안효원,강준구,손병용 대한상하수도학회 2004 상하수도학회지 Vol.18 No.2

DAF process has been designed considering raw water quality characteristics in Korea. Although direct filtration is usually operated, DAF is operated when the freshwater blooms occur or raw water turbidity become high. Pre-sedimentation is prepared in case when raw water turbidity is very high by rainstorms. A main feature of this plant is that the operation mode can be changed (controlled) based on the characteristics of raw water to optimize the effluent quality and the operation costs. Treatment capacity (surface loading rate) and efficiency of DAF was found to be better than conventional sedimentation process. Moreover, lowdensity particles (algae and alum flocs) are easily separated while it is difficult to remove in sedimentation. One of the main concerns in adoption of DAF (Dissolved-Air-Rotation) process is a high raw water turbidity problem. That is, DAF is not adequate for raw water, which is more turbid than 100MU. In order to avoid this problem, pre-sedimentation basins are prepared in OAF plant to decrease the turbidity of DAF influent. For simulation of the actual operation, bench and full-scale tests were performed for highly turbid water conditions. Consequently, DAF process coupled with sedimentation is suggested that pre-sedimentation with optimum coagulation prior to DAF would be appropriate.

전형적 징후를 동반한 결절성 경화증 1례

강순구,이중화,설종구 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

We report a case of tuberous sclerosis accompying the typical stigmata in a 18-year-old women who had undergone right nephrectomy. 40 to 80 per cent of tuberous sclerosis patients have renal angiomyolipoma. When angiomyolipoma is associated with tuberous sclerosis, the renal tumors usually are bilateral, muliple, samll and asymptomatic. Currently, it should be possible to make the diagnosis of angiomyoliporna in most cases on the basis of the combined imaging characteristics of CT and ultrasongraphy. Lesions associated with tuberous sclerosis require surgical intervention only when they become symptomatic or enlarge silently.

방광이행상피암에서 24시간 요중 Tissue Polypeptide Antigen(TPA) 및 Carcinoembryonic Antigen(CEA)의 임상적 가치

강순구,이중화,설종구 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

Tumor markers already play an important part in the management of the urologic neoplasm. As the number of emerging markers increases, clinical judgement may be an even more important part of the use of tumor markers. Ideally, a tumor marker should be produced reliably only by a specific malignancy, be easily and reproducibly measured, be detectable when a tumor is in its early stages and correlate with a response to therapy. Unfortunately, an ideal tumor marker has not yet been identified. Tissue polypeptide antigen(TPA) is heterogeneous in nature and SDS-polyarcrylamide gel electrophorsis resolves it into components with molecular weights raging from 45,000 to less than 20,000. Thus TPA is capable of being filtered through the kidney and excreted in the urine. The increased levels of TPA in urine and serum are probably due to a more rapid turnover and autolysis of cells and may parallel a more aggressive tumor behavior. Carcinoembryonic antigen(CEA) is a glycoprotein first found in extracts of human colonic cancer and the colon of human fetus during the first trimester of pregnancy. Initially it was considered that plasma CEA elevations were found only in case of endodermally-derived carcinomas. As bladder is, in part, endodermally-derived, this led Hall et al. to look for raised plasma and urine CEA levels in patients with urothelial carcinoma. We measured the TPA and CEA levels of 24-hours urine from 20 healthy individuals and 96 patients with definitive transitional cell carcinoma of the urinary bladder. And we evaluated the corelationships with grades, stages and recurrence of transitional cell carcinoma of the urinary bladder. The results are as follows; 1. Positive values(mean+2S.D.) of TPA and CEA levels of 24-hours urine showed significant differences between normal individuals and patients with transitional cell carcinoma of the urinary bladder(p<0.001 and p<0.05, respectively). 2. TPA and CEA levels in 24-hours urine showed significant differences between normal individuals and patients with transitional cell carcinoma of the urinary bladder(p<0.001 and p<0.05, respectively). 3. TPA levels in 24-hours urine of patients with transitional cell carclinoma of the urinary bladder showed significant difference in each grades or each stages. 4. CEA levels in 24-hours urine of patients with transitional cell carnoma of the urinary bladder did not show significant difference in each grades or each stages. 5. The recurrence rate in stage A patients whose 24-hours urine TPA level were above the positive value was 77%. The recurrence rate in stage A patients whose 24-hours urine CEA level were above the positive value was 66.7%. So, TPA level of 24-hours urine in patients with transitional cell carcinoma of the urinary bladder can be a good tumor marker and show the more close corelationships with tumor grades or stages than CEA levels of 24-hours urine in patients with transitional cell carcinoma of the urinary bladder.

316L 스테인리스강의 인장 및 저주기피로 변형시 관찰되는 동적변형시효 현상

홍성구,이순복 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.10

Tensile and low-cycle fatigue (LCF) tests were carried out in a wide temperature range from 20℃ to 750℃ at strain rates of 1×10^(-4)~1×10^(-2)/s for 17% cold worked 316L stainless steel to investigate the conditions for the occurrence of dynamic strain aging (DSA) and its effects on material properties during tensile and LCF deformations. DSA introduced anomalous changes of tensile and LCF properties, and the DSA regimes under tensile and LCF loading conditions coincided with each other. During tensile deformation, DSA can be manifested in the forms of the plateau in the variation of strength with temperature, the minima in the variation of ductility with temperature, serrated yielding in the stress-strain curves, and the negative strain rate sensitivity (SRS). In case of LCF deformation, it can be manifested in the forms of the occurrence of the plateau or the peak in the variation of cyclic peak stress with temperature, the negative temperature dependence of plastic strain amplitude or softening ratio, the negative SRS, and the negative strain rate dependence of plastic strain amplitude or softening ratio.

316L 스테인리스강의 고온 저주기 피로저항에 미치는 동적변형시효의 영향

홍성구,이순복 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.2

Mechanism of dynamic strain aging (DSA) and its effect on the low-cycle fatigue (LCF) behavior in type 316L stainless steel were investigated by performing LCF tests in air in a wide temperature range from 20 to 650℃ with strain rates of 3.2x10^(-5)-1x10^(-2)/s. The regime of DSA was evaluated using the anomalies associated with DSA and was in the temperature range of 250-550℃ at a strain rate of 1x10/s, in 250-600℃ at 1x10^(-4)/s, and in 250-650℃ at 1x10^(-2)/s. The activation energies for each type of serration were about 0.57-0.74 times those for lattice diffusion indicating that a mechanism other than lattice diffusion is involved. It seems to be reasonable to infer that DSA is caused by the pipe diffusion of solute atoms through the dislocation core. Dynamic strain aging reduced the crack initiation and propagation life by way of multiple crack initiation, which comes from the DSA-induced inhomogeneity of deformation, and rapid crack propagation due to the DSA-induced hardening, respectively. (Received October 29, 2004)