A study of delayed tooth autotransplantation after long-term cryopreservation with platelet-rich plasma in dogs

( Sool-heon Lee ),( Seong-jun Ham ),( Jeong-in Choi ),( Young-joon Kim ),( Jong-wook Moon ) 조선대학교 치의학연구원 2022 Oral Biology Research (Oral Biol Res) Vol.46 No.2

Tooth autotransplantation refers to tooth transplantation from the donor to the recipient site of the same individual. Occasionally, there are situations where autotransplantation cannot be carried out immediately at clinic. In such cases, donor teeth cryopreservation is necessary to overcome limited indications. Cryopreservation media and nutrients play essential roles in preserving the periodontal ligament cells’ activity. Several studies have focused on cryopreservation media and nutrients. Platelet-rich plasma (PRP) contains lots of growth factors without inducing immunologic responses or pathologic complications. Therefore, self serum and PRP might be positive factors in the cryopreservation process for preserving the periodontal ligaments (PDLs) viability. In this study, beagle dog's incisors were cryopreserved for three months in different cryopreservation media, including Dulbecco’s modified eagle medium (DMEM, containing DMSO), DMEM+20% self serum, DMEM+20% self serum+PRP, and DMEM+20% fetal bovine serum (FBS). Histological results revealed that DMEM+20% self serum+PRP and DMEM+20% FBS groups showed significantly less root resorption and higher regeneration of periodontal ligament cells and that PRP is a positive factor in the cryopreservation process to preserve PDLs viability. Therefore, cryopreservation media with PRP will be a good periodontal tissue regeneration option after long-term cryopreservation in a tooth autotransplantation procedure.

IL-$1{\beta}$ 처리 백서 두개관 세포의 mineral trioxide aggregate에 대한 반응

이술현,박지일,김영준,Lee, Sool-Heon,Park, Ji-Il,Kim, Young-Joon 대한치주과학회 2009 Journal of Periodontal & Implant Science Vol.39 No.3

Purpose: The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell. Methods: In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-$1{\beta}$ on MTA was evaluated by ELISA and RT-PCR. Results: The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-$1{\beta}$ group and MTA+IL-$1{\beta}$ group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-$1{\beta}$ group and Glass+IL-$1{\beta}$ group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression. Conclusions: MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.

Surface and Cut-edge Corrosion Behavior of Zn-Mg-Al Alloy-Coated Steel Sheets as a Function of the Alloy Coating Microstructure

Min-Suk Oh,Sang-Heon Kim,Jong-Sang Kim,Jae-Won Lee,Je-Ha Shon,Young-Sool Jin 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.1

The effects of Mg and Al content on the microstructure and corrosion resistance of hot-dip Zn-Mg-Al alloycoated steel sheets were investigated. Pure Zn and Zn-based alloy coatings containing Mg (0-5 wt%) and Al (0.2-55 wt%) were produced by a hot-dip galvanizing method. Mg and Al addition induced formation of intermetallic microstructures, like primary Zn, Zn/MgZn2 binary eutectic, dendric Zn/Al eutectoid, and Zn/Al/MgZn2/ternary eutectic structures in the coating layer. MgZn2-related structures (Zn/MgZn2, Zn/Al/ MgZn2, MgZn2) played an important role in increasing the corrosion resistance of Zn-Mg-Al alloy-coated steel sheets. Zn-3%Mg-2.5%Al coating layer containing a large volume of lamellar-shaped Zn/MgZn2 binary eutectic structures showed the best cut-edge corrosion resistance. The analysis indicated that Mg dissolved from MgZn2 in the early stage of corrosion and migrated to the cathodic region of steel-exposed cut-edge area to form dense and ordered protective corrosion products, leading to prolonged cathodic protection of Zn- Mg-Al alloy-coated steel sheets.

응급의료에서 이용될 휴대용 디지털 X-ray 전원장치 개발

조동헌,구경완,양해술,Cho, Dong-Heon,Koo, Kyung-Wan,Yong, Hae-Sool 한국조명전기설비학회 2006 조명·전기설비학회논문지 Vol.20 No.1

X-ray 발생 장치는 X-ray를 촬영할 수 있는 곳에 장착하는 고정 방식과 환자가 있는 병실로 장치를 움직여 X-ray를 촬영할 수 있도록 하는 이동 방식으로 구분할 수 있다. 환자의 상태에 따라 이동 방식은 매우 유용할 수 있지만 병원 내의 AC220[V]를 사용해야 하는 제약이 있었다. 병원으로부터 원거리에 있는 응급환자를 진단하거나 대형 사고에 의한 재난에서 환자를 분류하는 경우 응급 센터의 의사의 역할이 매우 제한적이었다. 따라서 본 연구는 사고 현장이나 이동 중인 구급차 내에서 X-ray 촬영이 가능한 X-ray 전원 장치를 개발하였고 다음과 같은 특성을 얻을 수 있었다. 첫째, X-ray를 발생하기 위한 장치의 전원은 휴대가 간편한 밧데리(DC12[V])를 사용하였다. 둘째, 제어회로는 PIC16F84A를 사용하여 X-ray 발생 장치의 신뢰성을 확보하였고 기능을 다양하게 제공할 수 있었다. 이 특성을 적용한 휴대용 디지털 X-ray발생 장치는 사고 현장에서 X-ray를 촬영하고 환자의 정보를 응급센터에 전달하여 의사의 적절한 처방을 받는 미래 지향적인 응급의료체제가 가능하도록 기여할 것으로 기대된다. The existing X-ray generators are either ones which are settled at fixed places or ones which are movable to sick rum. Movable generators can be very useful according to the circumstances of patients, but there remains a restraint that AC220[V] in the hospitals must be provided. When examining a first-aid patient who stays distant from the hospital or when grouping patients caused by disaster, the services of doctors at emergency centers should be very restrictive. Hence, this study developed a portable digital X-ray power supply unit that are utilizable at the accident spot or in a moving ambulance. By using the nit, the information of patients can be transmitted to the emergency center on the spot and thereby doctors can make a correct diagnosis. The properties of the unit are as follows: First, portable batteries(DCl2[V]) are utilized as electric source for the wit. Second PIC16F84A is utilized as control circuit in order to guarantee considerable reliance and to provide various functions. This portable digital X-ray power supply unit is expected to contribute to the emergency medical service system to be more advanced.

원격응급시스템에 적합한 X-ray 장치 개발

조동헌,구경완,양해술,한만석,한상옥,Cho, Dong-Heon,Koo, Kyung-Wan,Yang, Hae-Sool,Han, Man-Seok,Han, Sang-Ok 한국조명전기설비학회 2006 조명·전기설비학회논문지 Vol.20 No.6

본 연구에서 개발된 X-ray 장치는 병원으로부터 원거리에 있는 응급환자를 진단하거나, 대형 사고에 의한 재난에서 환자를 분류하기 위한 목적으로 사용할 수 있도록 제작되었다. X-ray 사용 장소는 병원으로부터 먼 거리에 있기 때문에 AC220[V]를 사용할 수 없는 사고 현장이나, 이동 중인 구급차 내에서도 응급환자의 진료가 필요한 곳이다. 개발된 X-ray 장치는 다음과 같은 특성이 있다. 첫째, 콘덴서를 내장하여 구급차의 밧데리 전원 공급이 없는 경우에도 X-ray 촬영이 가능하도록 하여 산악지역이나 섬지역 등에서도 이용할 수 있도록 하였다. 둘째, DC 12[V] 전원으로 X-ray를 발생하여 촬영한 정보를 디지털 디텍터로 검출하여 파일 형태로 저장하여 사용할 수 있었다. 제어회로는 Pic16F84A를 사용하여 X-ray 발생 장치의 신뢰성을 확보하였고, 기능을 다양하게 제공할 수 있었다. 원격응급시스템에 적합한 X-ray 장치는 응급환자가 의사로부터 공간적으로 멀리 떨어져 있거나, 시간적으로 진단, 처방, 치료가 동시에 이루어지기 어려운 상황 등에 효율적으로 대처할 수 있는 장점이 있다. The X-ray unit developed by this study is to diagnose emergency cases which is too far from a hospital and to classify the patients. We have to use the X-ray in a ambulance or the scene of an accident where we cannot use the AC220 outlet because of the distance from a hospital as well. The X-ray unit developed has a characteristics as follows. First of all, as the unit has a condenser in itself where there is no electric supply, we can use the X-ray inspector in a mountain area or a island. Second, we can detect by digital detector the information taken by X-ray from DC 12[V] electricity and store as a form of file. A control circuit can secure the reliability of the X-ray unit by using the Pic16F84A X-ray and provide various functions. The X-ray unit which suits remote emergency system can be efficiently used for the emergent cases who is too far from a doctor and a hospital or in the situation where it is difficult to diagnose, transcribe and treat simultaneously.

MRI에서 화학적 이동 선택(CHESS) pulse에 의한 지방소거의 임상적 유용성

한만석,양해술,진경수,어익수,조동헌,Han, Man-Seok,Yang, Hae-Sool,Jin, Kyung-Soo,Eo, Ik-Soo,Cho, Dong-Heon 한국정보처리학회 2007 정보처리학회논문지B Vol.14 No.6

자기공명영상(Magnetic Resonance Imaging:MRI)은 물과 지방 사이에 화학적 이동 현상을 가지고 있고 지방에 의해 구조적 현상이 나타난다. 또한 지방은 기본적으로 강한 신호를 가지고 있어 잘못된 인공물을 형성한다. 그래서 지방소거(fat suppression)에 대해 연구하고자 한다. MRI에서 지방소거(fat suppression)방법만을 사용하여 진단하는데 약간의 어려움은 있지만 기본은 화학적 이동 선택 포화(chemical shift selective saturation : CHESS) pulse 방법을 이용하여 물과 지방 phantom을 가지고 지방소거(fat suppression)를 연구하는데 목적이 있다. 본 연구에서 CHESS pulse를 matlap 프로그램을 이용 Map pulse로 알맞게 design하고 Pre saturation을 하기 위한 pulse diagram이 가능하였고, fat phantom과 water phantom을 실험하여 지방소거 기법(fat saturation)의 효과를 볼 수가 있었으며 임상 적용 부위로 경추(C-SPINE), 요추(L-SPINE), 가습(BREAST)에 적용하여 의료영상진단에 있어 화학적 이동 선택 포화 CHESS pulse의 지방소거(fat suppression)는 유용한 방법임을 증명하였다.(충남대학병원 G.E 1.5T MR를 이용하여 실험하였다.) Magnetic Resonance Imaging(MRI) has chemical shift phenomenon between fat and water, and the phenomenon has influence on structure enclosed by fat. Strong signals emitted from fat often generate false artefact, which reflects the importance of fat suppression techniques. There have been a number of researches on fat suppression techniques, but using fat suppression method alone in MRI can cause difficultproblems in diagnosis. This paper aims to study a fat suppression method by Chemical Shift Selective saturation(CHESS). This research describes the theoretical background and the experiment on water and fat phantom with MR instruments. In the experiment, CHESS pulse was designed by utilising Matlap program, and the pulse diagram was generated for the Pre-saturation process. The experiment using water and fat phantom was applied to C-spine, L-spine and Breast, and produced successful fat suppression results. This experiment has proved that the CHESSpulse fat suppression is a very helpful technique in diagnosing medical imaging. This method is a robust and useful technique for both clinical and basic investigators..(Experiment with Chungnam national university hospital G.E 1.5T MR)

간헐적 간혈류차단의 적절한 주기에 관한 실험적 연구

심문섭,김해영,배영태,김동헌,문상은,설미영,손한철,권경술 釜山外科學會 1999 釜山外科學會誌 Vol.15 No.12

간헐적 간혈류차단의 적절한 주기에 관한 실험적 연구

심문섭(Mun-Sup Sim),김해영(Hae-Young Kim),배영태(Young-Tae Bae),김동헌(Dong-Heon Kim),문상은(Sang-Eun Moon),설미영(Mi-Young Seol),손한철(Han-Chul Son),권경술(Kyung-Sool Kwon) 한국간담췌외과학회 1999 한국간담췌외과학회지 Vol.3 No.1

Research Forum Oral Presentation : The effect of human dentin-derived hydroxyapatite coating on titanium on differentiation of osteoblasts

( Sang Hyun Lee ),( Kye Hee Wee ),( Yang Jin Lee ),( Young Suk Jung ),( Kook Hyun Bong ),( So Won Uhm ),( Sang Chul Kim ),( Hyong Suk Oh ),( Sool Heon Lee ),( Ok Su Kim ),( Young Joon Kim ),( Hyun Ju 대한치주과학회 2009 대한치주과학회 학술대회자료집 Vol.2009 No.1