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MicroRNA 452 regulates ASB8, NOL8, and CDR2 expression in colorectal cancer cells
Ji‑Su Mo,Soo‑Cheon Chae 한국유전학회 2021 Genes & Genomics Vol.43 No.1
Background MicroRNAs play important roles in the pathogenesis of human diseases by regulating target gene expression in specifc cells or tissues. Previously, we identifed microRNA 452 (MIR452), which was specifcally up-regulated in early stage human colorectal cancer (CRC) tissue. Objective The current study aims to identify and verify the target genes of MIR452 associated with CRC. Methods A luciferase reporter system was used to confrm the efect of MIR452 on ASB8, NOL8, and CDR2 expression. The expression levels of MIR452 and the target genes were evaluated by quantitative RT-PCR (qRT-PCR) and western blotting. Results We verifed the association between MIR452 and three genes, ASB8, NOL8, and CDR2, and showed that their transcripts were down-regulated by MIR452. Up-regulated MIR452 also down-regulated ASB8, NOL8, and CDR2 mRNA and protein levels in CRC cells. CDR2 protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues. Conclusions These results suggest that ASB8, NOL8, and CDR2 were target genes of MIR452 in CRC cells and that upregulated MIR452 in CRC tissue regulated ASB8, NOL8, and CDR2 expression during colorectal carcinogenesis.
Newcastle Disease Virus의 Fusion 단백 유전자의 제한효소분절형태(RELP)의 특이성
장경수,김지영,김석,김태용,송영환,허지연,김현수,송희종,전무형 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-
To evaluate the specificity of restriction fragment length polymorphism (RFLP) of Newcastle disease virus (NDV) fusion (F) protein gene for various NDV strains. F protein genes of NDV CBP-1 isolate, LaSota, B1 and Kyojungwon (KJW) were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and RFLP were examined. In RT-PCR using F primer sets for 1.7 kb F gene, the amplified products were observed in CBP-1, LaSota and B1 except KJW. However, F2 primer set for 521 bp yielded the amplified bands with all strains. The amplified F genes (1.7 kbp) were treatd with 15 different restriction enzymes to analyze RFLP and the cleavage patterns. CBP-1 showed various cleavage patterns with 8 enzymes, LaSota, with 11 enzymes and B1, with 11 enzymes. According to the cleavage patterns, the physical maps were constructed, and it was found that CBP-1, LaSota and B1 strains had specific cleavage sites with HinfI, XbaI and XhoI. These findings suggest that RFLP are worth applying in the diagnosis and molecular epidemiological studies of Newcastle disease.
捻轉法이 竝行된 鍼刺戟 및 電鍼刺戟이 鎭痛效果에 미치는 影響
Park, Dong-Seok,Kim, Ji-Hoon,Min, Byung-Il,Oh, Soo-Myung 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1
Dong-Seok Park¹, Ji-Hoon Kim¹ and Byung-Il Min, Soo-Myung Oh,³¹Department of Acupuncture and Moxibustion, College of Medicine, ²Department of Physiology, College of Medicine, And ³East-West Medical Research Institute, Kyung Hee University,Seoul, Korea. Analgesic Effect of Manual Acupuncture and Electroacupuncture combined with Twirling Method. Proceeding of International Symposium on East-West Medicine Seoul. 187-200, 1999.-Twirling the needle (TN) is one of several needing methods frequently used for acupuncture in the oriental medicine. The present study was conducted to see if TN enhanced the antinociceptive effect produced by plain-or electroacupuncture in the rat tail flick test. Rats (Sprague-Dawley, 200-300g)lightly anesthetized with thiopental sodium(40mg/kg, i.p.) were used in the tail-flick test. The basal reaction time for tail-flick was 2 sec. For "plain" acupuncture(PA), a needle was inserted for 20 min into a Chock-samni (ST36) acupuncture point which (is located at the anterior tibial muscle and about 10mm below the knee joint). For electroacupuncture(EA), train-pulses(3 Hz,0.3-ms pulse width, 0.2-0.3mA) were applied to the inserted needle for the period of insertion. For combining TN with either PA or EA, TN was performed during the each acupuncture period. Two methods for TN were used; twirling 3 times for 1 second persisted for 1 min in every 5 mins(long-duration and long-interval;LDLI) and twirling 3 times for 1 second persisted for 10 sec in every 1 min(short-duration and short-interval;SDSI). PA and EA produced an increase in tail flick latency (TFL); peak increases were 21.3±5.1% and 42.1±17.3% of the pre-acupunture control, respectively, and occurred immediately after cessation of 20min-acupuncture. Performing TN combined with PA increased TFL more than PA itself by showing a greater peak increase in TFL when performed with a SDSI-TN than with a LDLI-TN(60.6±12.5% and 42.7±22.7% of the pre-acupuncture control, respectively). Performing TN combined with EA also increased TFL more than EA itself and a greater peak increase in TFL was observed with a SDSI-TN as compared to a LDLI-TN(77.5±13.8% and 67.3±14.0% of the pre-acupuncture control, respectively). These results indicate that TN enhances both PA-and EA-produced antinociception where an greater enhanced antinociception than a LDLI-TN. It is suggested that performing a SDSI-TN combined with EA seems to be one of the most effective ways to produce analgesia.
태권도 세계화와 교육적 가치 : Globalization of Taekwondo and its educational value
김지수,최종필,전태준 남서울대학교 2008 남서울대학교 논문집 Vol.14 No.2
Taekwondo became a world-wide sport beyond the symbol of Korean traditional culture. Taekwondo go through its value and put love for humanity and love of peace in practice in order to share love and participation with a citizen of the world. There are three essential prerequisite essentials for Taekwondo to be loved by people from all of the world in a globalizational era. Those are renovation, reinforcement, and solidarity and it can be practiced through revision of match rules, improvement of equipment, continuance of exhibition tour, and dispatch of masters.Also, educational values of Taekwondo is very important to achieve peace and happiness of all mankinds. Those are follows;First, Taekwondo has a basis of education for the whole man. Second, Taekwondo masters have authority as a leader. Third, Taekwondo training places are educational sites. Last, juveniles who train Taekwondo can get not only self-defense ability but cultivation of sound spirit. Key Words : Taekwondo, Globalization, Educational value
아시아 경제위기와 외환위기의 원인과 그 대응에 관한 평가
류지수 영남대학교 사회과학연구소 2001 社會科學硏究 Vol.21 No.1
본 논문은 1997년에 발생한 아시아 경제위기에 대해서 고찰하였다. 한국이 IMF의 구제 금융을 받게된 것을 계기로 IMF는 아시아 경제위기의 치유에 주도적인 역할을 맡게 되었다. 그러나 IMF의 구제금융은 아시아의 경제위기를 해결하지 못하였다. 구제금융의 지원에도 불구하고 이들 3개국의 신용등급은 더욱 멀어졌고 통화가치는 빨리 떨어져 외채위기는 더욱 증폭되어 IMF의 목표가 완전히 실패로 돌아갔다.
지보경,육동원,김지수,신정택 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2
The purpose of this study was to investigate coordination structure of infront kick in women soccer as a function of skill level. To succeed this purpose, four girls' high school students who had never experienced soccer before were selected as novices, and four girls' high school students who had played soccer for over three years as experts in this lab. To recognize the change of coordination structure according to practice, novices had practiced infront kick in soccer for two weeks. consequently they were taken films of kicking motion three times -before practice, after one week practice, after two weeks practice. However, experts were taken once. The experimental instruments for this study consisted of a video camera, reference frame. LED and APAS for digitizing. Angle to angle diagrams and phase plan portraits for qualitative analysis of coordination structure were gotten with LabVIEW 6i. The conclusions are as follow: firstly, novices' coordination structure had been changed since practice. Secondly, there were significantly different between coordination structures of experts and pre-practice novices. Lastly, there were similar between coordination structures of experts and post practice novices.