인체구강상피세포의 배양조건에 대한 연구

박종수,최희웅,이수관,김수복,고광삼 中央醫學社 1990 中央醫學 Vol.55 No.2

Human epithelial cell cultures were initiated from explants of normal human oral mucosa. Growth parameter and cell type were assessed by DNA concentration and morphology of the cell lines. Tissues were excised and epithelial cells isolated by collagenase digestion. Spermine was used in order to inhibit overgrowth of the epithelial cells by fibroblast. The effects of different medium formulations were compared. Cultures maintained on the same volume of RPMI 1640 and Dulbecco's modified minimum essential medium containing 10 % fetal bovine serum, 10 ?g/ml of insulin, lng/ml of somatomedin and 10 ng/ml of epidermal growth factor showed optimal and maximal growth. For each cultured human oral epithelial cell the DNA content was in the- range of 5.7 pg to 6.6 pg. This culture system provides epithelial cells that can be used for a variety of dental and biochemical studies.

허혈성 흰쥐 해마에서 NMDA수용체 아단위 NR2B와 세포골격단백질 MAP-2의 변화

정용욱,문일수,고복현 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

전반적 저산소증(global ischemia) 과 불완전 국소허혈(incomplete focal ischemia)에서 신경세포의 NMDA수용체 아단위 2B와 세포골격단백질 MAP2의 변화를 알아보기 위하여 immunoblot방법과 면역 조직화학법으로 조사하여 다음과 같은 결과를 얻었다. 1. 해마신경세포의 연접이후치밀질(postsynaptic density, PSD) 를 이용한 NR2B, MAP2표현의 변화를 알아보면 저 산소증과 불완정 국소허혈에서 3일 후 감소 하다가 6일 후에는 점차 정상 수준으로 환원되었으며(저 산소증의 MAP2제외)반대편 해마의 경우 NR2B는 3일경과 후 표현이 감소 하다가 6일 후에는 정상 수준 이상으로 증가 하였으며 MAP2는 정상과 변화가 없었다. 2. 해마의 면역조직화학법에서 MAP2의 표현은 저 산소증과 동측의 불완전 국소허혈 부위의 CA1, CA3, DG 전체 지역에서 정상에 비해 3일 후 감소 하다가 6일 후 증가 하였다(허혈의 반대측 해마의 경우는 CA1에서 MAP2의 표현이 6일째 감소됨.) 이상의 관찰 결과를 종합 해볼때 해마 신경세포의 지연성 손상은 NMDA수용체 아단위 2B의 감소와 세포골격단백질 MAP2를 파괴하며 그 시기는 3일 이전에 일어나 6일경에는 회복하는 단계를 거치는 것으로 생각되며 정확한 회복 시기는 허혈의 정도에 따라 차이가 있을 것으로 생각된다. 또한 허혈 반대편 부위에서의 NR2B와 MAP2의 면역 반응성 감소는 허혈지역의 주변부위에서(penumbra)에서 나타나는 현상과 일치하였다. Changes of NR2B & MAP-2 expression in global and focal ischemia Department of Anatomy, School of Medicine, DongGuk University YONG WOOK JUNG M.D., IL SOO MOON Ph.D., BOK HYUN KO M.D Microtubule associated protein 2 (MAP-2) degradation by Ca2' - dependent proteases after NMDARs (NRs) activation has been postulated in delayed hippocampal neuronal damage.. Degradation of MAP-2 in rat brain after hypoxia and ischemia was investigated through immunoblot analyses and immunohistochemistry. In order to test the changes of each NR subunits (NR2B) and MAP2 in neuronal damage, we carried out in vivo experiments to see if there is selective changes of MAP-2 and NR2B in global hypoxia and focal left common carotid artery ligation.. We found that, in global ischemia, NR2B was down-regulated and recovered to normal level by 3 and 6 days while MAP-2 was unchanged by the same days compared to untreated hippocampus, and that similar changes regarding to NR2B and MAP-2 were observed at ipsilateral hippocampus in focal ischemia. In contralateral hippocampus, NR2B expression was decreased and MAP-2 expression was unchanged compared to untreated hippocampus. These results imply that NR2B and MAP2 expression are regulated on the degree of ischemic damage in delayed neuronal death and that, in focal ischemia, down-regulation of NR2B in contralateral hippocampus may be a similar effect which is seen in the peripheral zone (penumbra) of ischemic region.

에타놀 攝取가 運動選手의 肺機能에 미치는 影響

허복,黃樹寬,朱永恩 慶北大學校 師範大學 1986 敎育硏究誌 Vol.28 No.-

In an effort to elucidate the effect of ethanol on the pulmonary function in athletes, 26 athletic college students were selected. Pulmonary function test was performed after administration of ethanol to the blood ethanol level of 40㎎/㎗. Results of the test were summarized as follows: Forced expiratory volumes were decreased by ethanol, and FVC and FEV_0.5, in particular, showed a significant decrease. Forced expiratory flows were decreased by ethanol. Especially, PEF, PEF_25%, FEF_(200∼1200㎖), and FEF_(25∼75%) showed a significant decrease. MVV was decreased by ethanol ingestion. Alveolar PO_2 and PCO_2 were not changed by ethanol ingestion. Blood pH, PCO_2, PO_2, bicarbonate, base excess, O_2 content and O_2 saturation showed no significant change.

사이버마케팅의 상호작용성에 관한 고찰

차수련,이흥복 동국대학교 경제경영연구원 1997 經濟經營硏究 Vol.21 No.1

Recent radical development of intelligence technology is changing marketing environments so fundamentally that we see a birth of new marketing system, that is, so called Cybermarketing system. Exchange in Cybermarketing system is free from temporal and spacial limits. Moreover time span is enlarged and interactions among existing market participants are much more deepened in this system. For consumers, more objective informations will be available and more control over information flows will be achieved, will overcome the information unbalanced between consumers and marketers. The advent of commercial online services and the far reach of the Internet have, for the first time in mass marketing history, created the potential for a mass interactive dialogue. The traditional marketing paradigm, a system of mass production, mass media, and mass marketing, is being replaced by a totally new paradigm, a one - to - one communication. The one-to-one communication will be characterized by customized product. This article offers a construct to aid markekter charged with maintaining a customer focus in an interactive communication. Utilizing what we call the Seven I's (e.g. Interconnection, Interface, Interactivity, Involvement, Information, Individualism and Integrity) marketers can better analyze and prepare for their transition from the marketplace to the marketspace. Because traditional marketing system has been based on one-way communication, there is no opportunity to exchange is ideas between marketer and consumer, no feedback from consumer to marketer. But Internet is a communication option that can be used to present database information to users and to capture information for into a database. As interactive communication evolves, the entire paradime of database marketing is being turned on its head because database consuming is being born. Database consuming is the result of customers using database technology to target products just as marketers have been using databases to target customers. Database consumers, and a result of the advances in information technology. have access to unprecedented tools that enable them to search out almost instantly vast amounts of information on products and service offered throughout the globe. Cybermarketing has a lot to offer when compared to traditional forms of marketing. It's not about to replace traditional advertising, direct marketing. and face to face selling bus it will extend it and expand traditional marketing in new direction and in way that will benefit both buyers and sellers. Concludly, it will be expected that interactivity of Cybermarketing contributes forward marketing system in various ways.

尹東柱의 詩의 世界認識과 自我同一性

金秀福 단국대학교 1994 論文集 Vol.28 No.-

The aim of this paper is to observe Dong-joo Yoon's world-recognition, the loss of self-identity and his attitude of recovering it. The imaginary structure of the world-recognition and self-identity in his poetical world is closely associated with the periodic situation of the age. The aspiration toward self-consciousness under the oppressed actual world reveals the attitude trying to overcome the sufferings and suppressions of the period, achieving the self-identity. The symbolic images diffused as a deep structure in his poetry(night, room, period, star, self) contain the historically-minded meaning of searching for self-identity under the oppressed actual world. Now, I would like to summarize my points as follows : 1. The world-recognition reflected in Dong-joo Yoon's poetry reveals introvert attitude. Negating the present time and dreaming of the reconciled world, his consciousness includes the view of world toward the future. Accordingly, he shows the introvert attitude trying to achieve the self-identity through the view of world toward the future, negating the periodic situation. 2. He recognized the actual world as the age of losing the self-identity. Grasping that the suffering and oppressed world as unable to the historically continued, recognizing such a situation as the age of losing the self-identity, he regarded such a periodic situation as the world without the present time and space of life. Accordingly, the tried to move toward 'the reminiscent native place of his childhood', the universal space of life, that is, the reconciled world of stars for the purpose of overcoming his own anxieties and conflicts in the world. 3. For the purpose of overcoming the world without self-identity, he shows the positive attitude to strengthen the existential consciousness of self-identity. Namely, he tried to recover the continuity with the diachronic world in order to overcome the oppressions and sufferings of life. That is to move toward the world dreaming of the harmonius reconciliation of self and object. This attitude can reconcile the opposite consciousness between self and world, moving toward the transcendental imaginary world. The represents the world of self-identity trying to to do his periodic duty given to his in his people's gloomy situation.

현대무용의 Leap 동작에서 숙련자와 비숙련자간의 운동학적 변인 비교 연구

김복영,배성제,정철수,이정은,임규찬 師範大學 體育硏究所 1992 서울大學校 體育硏究所論集 Vol.13 No.1

The purpose of this study was to compare kinematics of the skilled group with the corresponding of the unskilled group during the leap of the mordern dance and to investigate the effective movement patterns of the leap. The kinematic and temporal variables examined using the DLT(Direct Linear Transformation) method of three-dimensional cinematography were leap time, leap distance, leap height and body segment angles at the instants of take off, peak height and touch down. Four skilled and four unskilled groups were selected as the subjects. Two 16mm high speed cameras were used to monitor the subjects’movement patterns. T-test was used to determine if there is any significant (p<0.05) differences on kinematic variables between the skilled and unskilled groups. The conclusions obtained from the analyzed data were as follows; (1) There were not any significant differences between two groups for the angles of body segment at the instant of take off and leap distance. (2) At the instant of peak height, the leap angle and the left ankle angle for the skilled groups were larger than for unskilled groups. (3) At the instant of touch down, the trunk angle in the sagittal plane for the skilled groups were larger than for unskilled groups. (4) The flight time and height for the skilled groups were longer and higher than for the unskilled groups.

교실영어의 효율적인 사용 전략

선규수,조초희,조복희 광주교육대학교 초등교육연구소 2000 初等敎育硏究 Vol.15 No.1

This article proposes some strategies for effective use of classroom English in the EFL classroom. For this purpose, it argues the relationships between the use of classroom English and classroom language teaching. Through the use of classroom English, teachers of English should, whether native or non-native, help pupils progress from dependence on the book and on the teacher to independence, which is often called autonomy. The present article also analyzes the problems of current studies of classroom English. Particularly, the teachers of English who they think have limited proficiency of English and who teach young pupils in the EFL context may find that those studies rarely provide them with relevant information on classroom language teaching. A coherent, principled framework of classroom English needs to be proposed so that non-native speaker teachers of English can effectively use it for their English language teaching, depending on their students' age. The strategies thus developed are strongly supported by communicative language teaching in the EFL context.

<한> 思想의 哲理와 人類平和福祉主義

金福洙 韓國政治學會 釜山支會 1987 부산정치학회보 Vol.1 No.1

동남참게, Eriocheir japonicus (De Haan)의 種苗生産에 關한 生物學的 基礎硏究 : 成長段階別 幼生에 對한 脫皮 間隔日數에 미치는 水溫-鹽度의 複合的인 影響에 關하여 Combined Effects of Temperature-Salinity on the Moulting Intervals of Larvae

權晉洙,李福奎 동의대학교 기초과학연구소 1995 基礎科學硏究論文集 Vol.5 No.1

In order to study the optimum environmental condition for larvae culture of the freshwater crab, Eriocheir japonicus, larvae from different growth stages and young crab were cultured under the 16 different conditions of 4×4 temperature-salinity combinations (4 different temperatures at 22, 24, 26, 28℃ with 10.5, 17.5, 24.5, and 31.5‰ of salinity). The duration of metamorphosis, metamorphosis rate, the interval of. moulting period, and survival rate were measured from each experimental group of larvae and young crab under the different conditions. The results indicated that the optimum condition may be a 24.5‰ of salinity at water temperature at 22, 24, and 26℃. At 28℃ with 24.5‰, the duration of metamorphosis reduced somewhat, nevertheless metamorphosis and survival rate decrease a lot. And the lower the salinity showed the lower the metamorphosis and survival rates at 28℃.

멀리뛰기 발구름 동작시 아킬레스건각이 도약속도와 도약각도에 미치는 영향

김복영,배성제,신인식,정철수,임규찬 師範大學 體育硏究所 1992 서울大學校 體育硏究所論集 Vol.13 No.2

The purpose of this study was to investigate the effect of lower leg planting angle an on take-off motion of the long jump. The Achilles tendon angle defined as the angle between shank and calcaneus with projection to XY plane was introduced to interprete lower leg motion uring last foot touch for the flight phase of long jump. A typical high speed film analysis technique including DLT method was adapted to record and analyze total of nineteen long jumps performed by elite athletes at two different track and field events. The following were found that: 1. The relationship between touch-down velocity, take-off velocity, range of vertical velocity and flight length were significantly related each others as r=0.6383, 0.6395 respectively at t=0.01 level. 2. It was not possible to reveal the relationship between take-off angle and flight length. Due to highly consistent take-off angles shown between the long jumpers. 3. The angular displacement of the shank during the last foot contact just before flight phase was negatively related to the flight length(r= -0.6149). However, it was not possible to find the relationship between the aehilles tendon angle and flight length. 4. As the range of the body C.O.G lean angle increased the last step foot contact time significantly increased(r=0.8625). 5. The foot contact time of Korean elite long jumpers was somewhat longer than that shown by foreign Athletes. It is concluded that maintaining touch-down velocity during initial phase of foot touch and increasing take-off velocity would result optimal take-off angle and good record. Decrease in the angular displacement of the shank, foot contact duration, and the range of body C.O.G lean angle were found to be important to improve the flight length.