Esophageal Motor Dysfunctions in Gastroesophageal Reflux Disease and Therapeutic Perspectives

( Sihui Lin ),( Hua Li ),( Xiucai Fang ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.4

Gastroesophageal reflux disease (GERD) is a very common disease, and the prevalence in the general population has recently increased. GERD is a chronic relapsing disease associated with motility disorders of the upper gastrointestinal tract. Several factors are implicated in GERD, including hypotensive lower esophageal sphincter, frequent transient lower esophageal sphincter relaxation, esophageal hypersensitivity, reduced resistance of the esophageal mucosa against the refluxed contents, ineffective esophageal motility, abnormal bolus transport, deficits initiating secondary peristalsis, abnormal response to multiple rapid swallowing, and hiatal hernia. One or more of these mechanisms result in the reflux of stomach contents into the esophagus, delayed clearance of the refluxate, and the development of symptoms and/or complications. New techniques, such as 24-hour pH and multichannel intraluminal impedance monitoring, multichannel intraluminal impedance and esophageal manometry, high-resolution manometry, 3-dimensional highresolution manometry, enoscopic functional luminal imaging probe, and 24-hour dynamic esophageal manometry, provide more information on esophageal motility and have clarified the pathophysiology of GERD. Proton pump inhibitors remain the preferred pharmaceutical option to treat GERD. The ideal target of GERD treatment is to restore esophageal motility and reconstruct the antireflux mechanism. This review focuses on current advances in esophageal motor dysfunction in patients with GERD and the influence of these developments on GERD treatment. (J Neurogastroenterol Motil 2019;25:499-507)

Esophageal Motility Disorders in Gastroesophageal Reflux Disease and Therapeutic Perspectives

Sihui Lin,Hua Li,Xiucai Fang 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.4

Gastroesophageal reflux disease (GERD) is a very common disease, and the prevalence in the general population has recently increased. GERD is a chronic relapsing disease associated with motility disorders of the upper gastrointestinal tract. Several factors are implicated in GERD, including hypotensive lower esophageal sphincter, frequent transient lower esophageal sphincter relaxation, esophageal hypersensitivity, reduced resistance of the esophageal mucosa against the refluxed contents, ineffective esophageal motility, abnormal bolus transport, deficits initiating secondary peristalsis, abnormal response to multiple rapid swallowing, and hiatal hernia. One or more of these mechanisms result in the reflux of stomach contents into the esophagus, delayed clearance of the refluxate, and the development of symptoms and/or complications. New techniques, such as 24-hour pH and multichannel intraluminal impedance monitoring, multichannel intraluminal impedance and esophageal manometry, high-resolution manometry, 3-dimensional high-resolution manometry, enoscopic functional luminal imaging probe, and 24-hour dynamic esophageal manometry, provide more information on esophageal motility and have clarified the pathophysiology of GERD. Proton pump inhibitors remain the preferred pharmaceutical option to treat GERD. The ideal target of GERD treatment is to restore esophageal motility and reconstruct the anti-reflux mechanism. This review focuses on current advances in esophageal motor dysfunction in patients with GERD and the influence of these developments on GERD treatment.

GAS5 regulates viability and apoptosis in TGF-β1-stimulated bronchial epithelial cells by regulating miR-217/HDAC4 axis

Zhao Sihui,Ning Yunfang,Qin Na,Ping Nan,Yu Yong,Yin Guoyan 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background Asthma is a serious respiratory disease that afects the physical and mental health of children. Airway epithelial apoptosis concomitantly mediated by transforming growth factor-β1 (TGF-β1) is a crucial component of asthma pathogenesis. LncRNA growth Arrest Specifc 5 (GAS5), microRNA-217 (miR-217) and Histone deacetylase 4 (HDAC4) shown a close relationship with TGF-β1-induced injury of airway epithelial. However, the mechanism underlying TGF-β1-induced injury of airway epithelial in asthma still needs to be investigated. Objective We aimed to investigate the efect and underlying mechanism of GAS5/miR-217/HDAC4 axis in TGF-β1- stimulated bronchial epithelial cells. Methods The levels of were detected by quantitative real-time polymerase chain reaction (RT-qPCR). All protein levels were determined by western blot. Cell viability and apoptosis rate were assessed by Methyl thiazolyl tetrazolium (MTT) and Flow cytometry, respectively. The targeting relationship between miR-217 and GAS5 or HDAC4 was examined with dual-luciferase reporter assay. Results TGF-β1, GAS5, HDAC4 were up-regulated, while miR-217 was down-regulated in bronchial mucosal tissues of asthmatic children and TGF-β1-treated BEAS-2B cells. TGF-β1 could reduce cell viability and induce apoptosis, while these efects could be reversed by downregulation of GAS5 or HDAC4. Mechanically, GAS5 acted as a sponge for miR-217 to regulate the expression of HDAC4. Furthermore, overexpression of HDAC4 rescued the efects of GAS5 knockdown on viability and apoptosis of TGF-β1-induced BEAS-2B cells. GAS5 knockdown induced cell viability and hampered cell apoptosis in TGF-β1-stimulated BEAS-2B cells by regulating the miR-217/HDAC4 axis. Conclusions The lncRNA GAS5/miR-217/HDAC4 axis played an important role in regulating TGF-β1-induced bronchial epithelial cells injury, thus contributing to asthma.

공공디자인에 기반으로 한 도시문화 관광에 관한 연구-부산3개 공원 휴게시설 유니버설디자인을 중심으로-

인스후이 ( Yin Sihui ),첨초비 ( Zhan Chaobi ),한맹맹 ( Han Mengmeng ),루정쥔 ( Lu Zhengjun ) 한국기초조형학회 2023 한국기초조형학회 학술발표논문집 Vol.2023 No.2

Universally Composable Attribute-based Group Key Exchange

Hui Xie,Yongjie Yan,Sihui Shu 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.1

Several protocols implementing attribute-based group key exchange, which allows users with certain set of attributes to establish a session key, have been proposed in recent years. However, attacks on attribute-based group key exchange in current research have been considered only in stand-alone fashion. Thus these protocols may be vulnerable when run with other protocol sessions concurrently. We treat the security of attribute-based group key exchange in the universal composability framework to ensure that a protocol remains secure when run with arbitrary protocol sessions concurrently. More specifically, we define an ideal functionality for attribute-based group key exchange first, then propose a two-round protocol based on a primitive called encapsulation policy attribute-based key encapsulation mechanism. In addition, a complete security proof of our protocol in the universal composability framework under random oracle model is given.

韩国学习者的汉语学习动机研究 -以学习者的一般特性与学习成绩的相关关系为中心

이은화,Sihui Ke 한국중국언어학회 2022 중국언어연구 Vol.- No.98

The purpose of this study was to examine the correlation between Korean learners’ motivation to learn the Chinese language and their general characteristics (gender, HSK rating, the number of acquired Chinese characters, period of studying in China, and period of learning the Chinese language) and the correlation between their motivation to learn Chinese and their academic achievement. For this study, with 25 survey questions regarding the motivation to learn the Chinese language based on Gardner’s (1985) “The Attitude and Motivation Test Battery” (AMTB) and precedent research, a survey targeting Korean undergraduate students was carried out and the results were analyzed. The correlation between Korean learners’ motivation to learn Chinese and their general characteristics was examined using ANOVA, Bonferroni, Linear Trend and Structural Equation Modeling (SEM) as the statistical analysis methods. Then, it was further examined on what factors continuously increased their motivation to learn by investigating whether a change in their general characteristics enhanced their motivation to learn Chinese and whether there was a linear relationship of proportional increase between such factors and their motivation to learn the Chinese language. In addition, direct and indirect effects of motivation to learn on Korean students’ academic achievement regarding the Chinese language were considered. Currently, there is a significant lack of investigation and studies on the intrinsic problems of learners who are the most important subject of Chinese education. However, identifying factors that are correlated with Korean learners’ motivation to learn Chinese and which motivation to learn has direct and indirect effects on their academic achievement regarding the Chinese language is very important and should be prioritized. The result of this study will help Korean learners successfully learn Chinese by using the correlation between Korean learners’ motivation to learn the Chinese language and their academic achievement more actively and efficiently in the future. 本研究以韩国大学生为研究对象进行了问卷调查,主要讨论了韩国学习者的汉语学习动机如何对他们的成绩产生直接或间接的影响。利用方差分析法等分析与学习动机有关的因素,利用线性趋势分析影响学习动机的因素,利用结构方程模型分析学习动机对学习成绩的影响。通过调查结果可知,韩国汉语学习者的学习动机和学习者的性别、HSK等级、汉字的识字量、学习汉语时间、中国留学时间之间有相关关系。韩国学习者的汉语学习动机和汉字的识字量、中国留学时间、学习汉语的时间之间有线性关系。即随着汉字的识字量、学习汉语时间的增加学习者的汉语学习动机强度也会随之增强。但是,随着HSK等级的提高或中国留学时间的增加,学习者的学习动机强度并不会因此增强。韩国学习者的学习动机中,对汉语学习成绩有直接影响的是‘喜欢学习汉语’动机,‘汉语的重要性’和‘工具性原因’对汉语学习成绩有间接影响。了解韩国学习者基于什么动机学习汉语,以及哪种动机会对学习成绩产生积极的影响,这是本文研究意义所在。

Association between Metabolic Syndrome and Microvascular Complications in Chinese Adults with Type 1 Diabetes Mellitus (Diabetes Metab J 2022;46:93-103)

Qianwen Huang,Sihui Luo 대한당뇨병학회 2022 Diabetes and Metabolism Journal Vol.46 No.3

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Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China

Jiao Yifan,Zhang Sihui,Yang Jing,Lai Xin-He,Dong Kui,Cheng Yanpeng,Xu Mingchao,Zhu Wentao,Lu Shan,Jin Dong,Pu Ji,Huang Ying,Liu Liyun,Wang Suping,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Two facultatively anaerobic, short rod-shaped, non-motile, Gram-stain-positive, unknown bacterial strains (JY-X040T and JY-X174) were isolated from fluvial sediments of Tongtian River in Yushu Tibetan Autonomous Prefecture, Qinghai province, China. Cells formed translucent, gray, round and convex colonies, with a diameter of less than 0.5 mm after 5 days of incubation at 30°C on brain heart infusion-5% sheep blood agar. The 16S rRNA gene sequence similarity between strain JY-X040T and Fudania jinshanensis 313T is 93.87%. In the four phylogenetic trees constructed based on the 16S rRNA gene and 423 core genes, the two isolates form an independent branch, phylogenetically closest to F. jinshanensis 313T, but could not be classified as a member of the genus Fudania or any other genus of the family Arcanobacteriaceae. The DNA G + C content of strain JY-X040T was 57.8%. Calculation results of average nucleotide identity, digital DNADNA hybridization value and amino acid identity between strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%, and 64.1%. The major cellular fatty acids were C16:0 (23%) and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α (L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and four unidentified components. The whole-cell sugars contained rhamnose and ribose. MK-10(H4) was the sole respiratory quinone. The minimum inhibitory concentration of streptomycin was 32 μg/ml. All physiological, biochemical, chemotaxonomic and genomic characteristics support that strains JY-X040T and JY-X174 represent members of a novel species in a new genus, Changpingibacter yushuensis gen. nov., sp. nov. The type strain is JY-X040T (GDMCC 1.1996T = KCTC 49514T).

Description of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments

Huang Yuyuan,Jiao Yifan,Zhang Sihui,Tao Yuanmeihui,Zhang Suping,Jin Dong,Pu Ji,Liu Liyun,Yang Jing,Lu Shan 한국미생물학회 2023 The journal of microbiology Vol.61 No.4

Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E) in July 2019. Both strains were shown to grow at 15–35 °C and pH 7.0–10.0, and in the presence of 0–6.0% (w/v) NaCl. The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6–98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5–98.6%) and O. murale 01-Gi-040T (98.3–98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0–23.9% and 70.8–80.4%, respectively, all below the respective recommended 70.0% and 95–96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin ( C40H56O) can be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).

Nattokinase Crude Extract Inhibits Hepatocellular Carcinoma Growth in Mice

( Yongmin Yan ),( Yanjing Wang ),( Jiali Qian ),( Sihui Wu ),( Yi Ji ),( Yanxiao Liu ),( Jian Zeng ),( Aihua Gong ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8

Nattokinase (NK, E.C. 3.4.21.62) is a serine protease produced by Bacillus subtilis natto that shows promise for the treatment of thrombotic disease. In this study, we assessed the effects of NK on the development of hepatocellular carcinoma (HCC), a principal malignancy of the liver that causes morbidity and mortality worldwide. Crude extracts of NK (NCE) were isolated from fermentation medium by centrifugation and separated into three fractions (< 10 K, 100~30 K and >30K). Orthotopic HCC mouse models were established and NCE was administered by oral gavage. H&E staining was performed to examine the pathology of HCC livers. Immunohistochemistry and immunofluorescence were used to evaluate FOXM1, CD31, CD44 and vimentin expression in the liver. Compared to PBS groups, NCE increased the survival rates of HCC-bearing mice to 31% and decreased ascites. Low-intensity ultrasound imaging showed that the hypoechoic mass area was lower in NCE-treated mice and that tumor growth significantly decreased. IHC staining showed that the expression of FOXM1 was inhibited by NCE treatment. Immunofluorescence results revealed lower levels of CD31, CD44 and vimentin in the NCE groups. Taken together, these data demonstrate that NCE from Bacillus subtilis natto improves survival and inhibits tumor growth in HCC mice.