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Selvarasu, Suresh,Ow, Dave Siak-Wei,Lee, Sang Yup,Lee, May May,Oh, Steve Kah-Weng,Karimi, Iftekhar A.,Lee, Dong-Yup Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and Bioengineering Vol.102 No.3
<P>Genome-scale flux analysis of Escherichia coli DH5α growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in the complex medium as well as the complete consumption of key carbohydrate nutrients, glucose and trehalose. Based on the consumption and production rates of the measured metabolites, constraints-based flux analysis of a genome-scale E. coli model was then conducted to elucidate their utilization in the metabolism. The in silico analysis revealed that the cell exploited biosynthetic precursors taken up directly from the complex medium, through growth-related anabolic pathways. This suggests that the cell could be functioning in an energetically more efficient manner by reducing the energy needed to produce amino acids. The in silico simulation also allowed us to explain the observed rapid consumption of serine: excessively consumed external serine from the complex medium was mainly converted into pyruvate and glycine, which in turn, led to the acetate accumulation. The present work demonstrates the application of an in silico modeling approach to characterizing microbial metabolism under complex medium condition. This work further illustrates the use of in silico genome-scale analysis for developing better strategies related to improving microbial growth and enhancing the productivity of desirable metabolites. Biotechnol. Bioeng. 2009; 102: 923–934. © 2008 Wiley Periodicals, Inc.</P>
Shock analysis of a new ultrasonic motor subjected to half-sine acceleration pulses
Hou, Xiaoyan,Lee, Heow Pueh,Ong, Chong Jin,Lim, Siak Piang Techno-Press 2016 Advances in computational design Vol.1 No.4
This paper aims to examine the dynamic response of a newly designed ultrasonic motor under half-sine shock impulses. Impact shock was applied to the motor along x, y or z axis respectively with different pulse widths to check the sensitivity of the motor to the shocks in different directions. Finite Element Analysis (FEA) with the ANSYS software was conducted to obtain the relative displacement of a key point of the motor. Numerical results show that the maximum relative displacement is of micro meter level and the maximum stress is five orders smaller than the Young's modulus of the piezo material, which proves the robustness of the motor.