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Improved Homologous Expression of the Acidic Lipase from Aspergillus niger
Si-Yuan Zhu,Yan Xu,Xiao Wei Yu 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.2
In this study, the acidic lipase from Aspergillus niger (ANL) was homologously expressed in A. niger. The expression of ANL was significantly improved by the expression of the native ANL with the introns, the addition of the Kozak sequence and the optimization of the signal sequences. When the cDNA sequence of ANL fused with the glaA signal was expressed under the gpdA promoter in A. niger, no lipase activity could be detected. We then tried to improve the expression by using the full-length ANL gene containing three introns, and the lipase activity in the supernatant reached 75.80 U/ml, probably as a result of a more stable mRNA structure. The expression was further improved to 100.60 U/ml by introducing a Kozak sequence around the start codon due to a higher translation efficiency. Finally, the effects of three signal sequences including the cbhI signal, the ANL signal and the glaA signal on the lipase expression were evaluated. The transformant with the cbhI signal showed the highest lipase activity (314.67 U/ml), which was 1.90-fold and 3.13-fold higher than those with the ANL signal and the glaA signal, respectively. The acidic lipase was characterized and its highest activity was detected at pH 3.0 and a temperature of 45ºC. These results provided promising strategies for the production of the acidic lipase from A. niger.
Protein Kinase A Regulates the Osteogenic Activity of Osterix
( Si Yuan He ),( You Hee Choi ),( Joong Kook Choi ),( Chang Yeol Yeo ),( Chang Ju Chun ),( Kwang Youl Lee ) 전남대학교 약품개발연구소 2014 약품개발연구지 Vol.23 No.-
Osterix belongs to the SP gene family and is a core transcription factorresponsible for osteoblast differentiation and bone formation. Activation of protein kinase A (PKA), a serine/threonine kinase, is essential for controlling bone formation and BMP-induced osteoblast differentiation. However, the relationship between Osterix and PKA is still unclear. In this report, we investigated the precise role of the PKA pathway in regulating Osterix during osteoblast differentiation. We found that PKA increased the protein level ofOsterix; PKA phosphorylated Osterix, increased protein stability, and enhanced the transcriptional activity of Osterix. These results suggest that Osterix is a novel target of PKA, and PKA modulates osteoblast differentiation partially through the regulation of Osterix. J. Cell. Biochem. 115: 1808-1815, 2014. ⓒ 2014 Wiley Periodicals, Inc.
A Study on the Design Characteristics of Public Space in the Silver Town
Si-zhou Yuan,Jae-cheol Kang ASCONS 2022 IJASC Vol.4 No.3
Background/Objectives: As the aging of the population becomes more serious, it is necessary to focus on how to improve the quality of life of the elderly in old age as well as solving the problem of the fertility rate. Although the design level of public space in Silver Town is different, a good Silver Town space may be preferred by the elderly and help to ensure psychological and physiological health. Methods/Statistical analysis: The purpose of this study is to derive the design characteristics of the elderly silver town public space through investigation and analysis. Findings: In order to achieve the purpose of the study, research methods such as review of existing literature, case analysis, and expert research were conducted. Through case analysis and expert interview survey, Silver Town public space design characteristics according to physiological factors, psychological factors, and social factors were summarized, and the final Silver Town public space design characteristics for each factor were derived through an expert 5-point scale evaluation survey. Improvements/Applications: As a result of the study, the Silver Town public space design characteristic according to the physiological factors of the elderly was 'safety', and the Silver Town public space design characteristic according to the psychological factors was 'Ease of use'. Lastly, the silver town public space design characteristic according to the social factors of the elderly is 'participation possibility'.
( Si-yuan Sun ),( Ying Zheng ),( Jun-hua Zhou ),( Jiu-xing Weng ),( Yi-fei Wei ),( Xiao-jun Wang ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.7
The requirements for powerful computing capability, high capacity, low latency and low energy consumption of emerging services, pose severe challenges to the fifth-generation (5G) network. As a promising paradigm, mobile edge networks can provide services in proximity to users by deploying computing components and cache at the edge, which can effectively decrease service delay. However, the coexistence of heterogeneous services and the sharing of limited resources lead to the competition between various services for multiple resources. This paper considers two typical heterogeneous services: computing services and content delivery services, in order to properly configure resources, it is crucial to develop an effective offloading and caching strategies. Considering the high energy consumption of 5G base stations, this paper considers the hybrid energy supply model of traditional power grid and green energy. Therefore, it is necessary to design a reasonable association mechanism which can allocate more service load to base stations rich in green energy to improve the utilization of green energy. This paper formed the joint optimization problem of computing offloading, caching and resource allocation for heterogeneous services with the objective of minimizing the on-grid power consumption under the constraints of limited resources and QoS guarantee. Since the joint optimization problem is a mixed integer nonlinear programming problem that is impossible to solve, this paper uses deep reinforcement learning method to learn the optimal strategy through a lot of training. Extensive simulation experiments show that compared with other schemes, the proposed scheme can allocate resources to heterogeneous service according to the green energy distribution which can effectively reduce the traditional energy consumption.
중국 IP(Intellectual Property)영화 작품의 창작 트렌드
Wei, Si-Yuan,Song, Seung-Keun 한국정보통신학회 2020 한국정보통신학회논문지 Vol.24 No.2
Recently, IP (Intellectual Property) films have attracted much attention in the Chinese film market. This raises the need to take intellectual property films seriously in academic terms. The purpose of this study is to explore the effective methodology of content development based on intellectual property by analyzing the recent trend of Chinese intellectual property films. Recently, the driving force of the development of Chinese intellectual property films was analyzed, and the trend of Chinese intellectual property films was analyzed. The results of this study criticized the fragmented problems caused by the development of intellectual property films. This study made practical and academic suggestions to contribute to the healthy development of the intellectual property film market.
ADPRT Val762Ala and XRCC1 Arg194Trp Polymorphisms and Risk of Gastric Cancer in Sichuan of China
Wen, Yuan-Yuan,Pan, Xiong-Fei,Loh, Marie,Tian, Zhi,Yang, Shu-Juan,Lv, Si-Han,Huang, Wen-Zhi,Huang, He,Xie, Yao,Soong, Richie,Yang, Chun-Xia Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Objective: Gastric cancer remains a major health problem in China. We hypothesized that XRCC1 Arg194Trp and ADPRT Val762Ala may be associated with risk. Methods: We designed a multicenter 1:1 matched case-control study of 307 pairs of gastric cancers and controls between October 2010 and August 2011. XRCC1 Arg194Trp and ADPRT Val762Ala were sequenced, and demographic data as well as lifestyle factors were collected using a self-designed questionnaire. Results: Individuals carrying XRCC1 Trp/Trp or Arg/Trp variant genotype had a significantly increased risk of gastric cancer (OR, 1.718; 95% CI, 1.190-2.479), while the OR for ADPRT Val762Ala variant genotype (Ala/Ala or Val/Ala) was 1.175 (95% CI, 0.796-1.737). No gene-gene or gene-environment interactions were found. In addition, family history of cancer and drinkers proportion were higher among cases than among controls (P<0.05). Conclusions: XRCC1 194 Arg/Trp or Trp/Trp genotype, family history of cancer, and drinking are suspected risk factors of gastric cancer from our study. Our findings may offer insight into further similar large gene-environment and gene-gene studies in this region.