烏貝散이 흰쥐 胃의 Gastrin, Histamine, Somatostatin 면역반응세포에 미치는 영향

이시섭,나현욱,고병문,이광규,이창현 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5

To investigate the elects on the administration of Opae-san in rats. Opae-san (500mg/day) and omeprazole(10mg/day) were administration with stomach tube for 4 weeks and 8 weeks. This experiment were investigated numerical change of immunoreactive cells of gastric, histamine and somatostatin in rat stomach mucosa by the immunohistochemical method. The results were as follows : 1. In Opae-san administration group for 4 weeks, the number of gastric immunoreactive cells were increased in one and a half times than that of control group. In omeprazole administration group for 4 weeks, the number of gastric immunoreactive cells were increased in four times than that of control group. In Opae-san administration group for 8 weeks, the number of gastric immunoreactive cells were increased four times than that of control group. In omeprazole administration group for 8 weeks, the number of gastrin immunoreactive cells were increased in six times than that of control group. 2. In Opae-san administration group for 4 weeks, the number of histamine immunoreactive cells were increased in two times than that of control group. In omeprazole administration group for 4 weeks, the number of histamine immunoreactive cells were increased in six times than that of control group. In Opae-san administration group for 8 weeks, the number of histamine immunoreactive cells were increased three times than that of control group. In omeprazole administration group for 8 week, the number of histamine immunoreactive cells were increased in seven times than that of control group. These results suggest that Opae-san extracts inhibit the secretion of gastric acid and this extract use to therapeutic herb of gastric disorders related to the hyperacidity and gastric ulcer.

아연계 탈황제의 장기연속 반응성 연구

박노국,이종욱,이승철,이종대,류시옥,이태진 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.2

The zinc titanate sorbents(ZTG40) were prepared and the tests for durability and attrition resistance were carried out to develop the highly effective desulfurization sorbents. In the attrition tests for ZTG40 the results showed that the attrition resistance was 94.08% and that the sulfur capacity was maintained above 15g S/100g sorbent throughout the 100-cycle test, its maximum being 23.05g S/100g sorbent. As compared to sorbents developed by the developed countries, ZTG40 showed superior performances. In the advanced countries 15g S/100g sorbent is a yardstick of excellency for the sulfur capacity of desulfurization sorbents. Therefore, ZTG40 developed in this study has the desirable physical and chemical properties for fluidized desulfurization of coal gases.

세균독소로 자극시킨 사람 섬유아 세포에서의 Interleukin-6와 Interleukin-8의 생성

정인교,양동규,홍시영,이성근,김욱규,김광혁,김종렬 大韓顎顔面成形再建外科學會 1999 Maxillofacial Plastic Reconstructive Surgery Vol.21 No.4

Cytokines are hormone-like proteins which mediate and regulate inflammatory and immune responses. Interleukin-6 (IL-6) is involved in the final differentiation of B cells into antibody-producing cells. Interleukin-8 (IL-8) is a neutrophil chemotactic factor that plays an important role in the recruitment of neutrophil to inflammatory loci. Inflammatory mediators by cells in the gingiva have been implicated in the initiation and progression of periodontitis and oral infection. The purpose of this study was conducted to investigate the effect of lipopolysaccharide (LPS), staphylococcus enterotoxin B (SEB) on production ofIL-6 and IL-8 by human gingival and facial dermal fibroblasts. Primary cultured human gingival and facial dermal fibroblasts were incubated with LPS(0.01, 0.1, 1.0㎍/ml), SEB (0.01, 0.1, 1.0㎍/ml) or LPS (0.1㎍/ml) plus SEB (0.1㎍/ml). Culture supernatants were collected at 24, 48, and 72 hrs and assessed for IL-6 and IL-8 production by enzyme-linked immunosorbent assay. IL-6 production in gingival fibroblasts stimulated with LPS was higher than that with SEB. IL-6 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-6 production in facial demal fibroblasts was increased only by stimulation with a high concertration of LPS (1.0㎍/ml). Its production in facial dermal fibroblasts by exposure with SEB was decreased in comparison with control, nontreated cells. Therefore, gingival fibroblasts showed higher sensitivity than facial dermal fibroblasts in response to low concentration of LPS. Also, IL-6 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB.IL-6 production in facial dermal fibroblasts was increased only by stimulation with a high concentration of LPS(1.0㎍/ml). Its production in facial dermal fibroblasts by exposure with SEB was decreased in comparison with control, nontreated cells. Therefore, gingival fibroblasts showed higher sensitvity than facial dermal fibroblasts in response to low concentration of LPS. Also, IL-6 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-8 production in gingival fibroblasts was enhanced greatly only by stimulation of high concentration of LPS (1.0㎍/ml). That by exposure with SEB was increased only in 24 hrs cultivation. IL-8 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-8 production in facial dermal fibroblasts was decreased by LPS and increased only in 48 hrs cultivation by SEB. IL-8 production by double exposure with LPS plus SEB was enhanced only in 48 hrs cultivation in comparison with single exposure of LPS or SEB. therefore, IL-6 and IL-8 production were released at various quantities according to bacterial toxin applied and site of fibroblast harvested. These results suggest that gingival fibroblasts may be concerned with IL-6 and IL-8 related inflammatory response more than facial dermal fibroblasts.

虹彩 體質 分析에서 心腎結合組織의 虛弱 體質과 高血壓 家族歷과 관련성 硏究

金鐘牛,黃祐準,琴坰樹,李始炯,李宗淳,都金錄,趙在運,趙州掌,金鐘煜 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

Iridology, developed more than 100 years ago, is the diagnosis of medical conditions through noting irregularities of the pigmentation in the iris The purpose of this study is to compare interrelation between CVA, hypertension family history and cardio-renal connective tissue in Iris Constitution through iris constitution examination. The subjects consist of 114 patients who were examined in Iris Constitution at Chonju Oriental Medicine Hospital attached to Wonkwang Univ. from September. 1St. 2000 to August. 31th. 2002. 1. In the distribution of Iris Constitution, among of 79cases, There are neurogenic type 13cases, abdominal connective tissue weakness constitution 31 cases, cardio-renal connective tissue weakness constitution 6 cases, cholesterol 7 cases and others 22 case in control group. 2. There are familial history of CVA and hypertention 32 cases, no familial history of CVA and hypertention1 3 cases, in 35cases of experimental group. These results imply that there is meaningfulness of interrelation between cardio-renal connective tissue weakness constitution and. CVA, hypertention.

Expression Patterns of vnd Gene in Larval and Adult Stages of Drosophila Melanogaster

Si Uk Yoo 한국유전학회 2009 Genes & Genomics Vol.31 No.3

The Drosophila ventral nervous system defective (vnd) gene encodes a homeodomain transcription factor which initiates neural development in the ventral portion of neuroectoderm. The functions of vnd in regulating neuroblast (NB) formation during embryonic nervous system development have been extensively studied, however, little is known about its roles during the larval and adult development. The core promoter region corresponding to the -5.3 to -2.8 kb from the 5`-flanking region of the vnd gene contains strong enhancers required for NB formation in Drosophila embryos. This region can be divided into two fragments, a distal element containing approximately 1.3 kb DNA fragment (-5.3 to -4.0 kb) and a proximal element carrying about 1.2 kb fragment (-4.0 to -2.8 kb). To study expression pattern of vnd gene after embryonic stage, transgenic lines carrying either a distal or a proximal element were generated, and the expression pattern of the vnd gene was observed by the expression of a green fluorescence protein (GFP) reporter gene. The transformants carrying either a distal or a proximal element show GFP expression in salivary gland, gut epidermis tissue, and testis during larval and adult stages. GFP expression was also observed in the larval brain from the transgenic line containing the proximal element; however, no expression was detected in the adult brain. To identify an enhancer region responsible for the vnd expression, the transgenic lines carrying various deletions within the promoter region were generated. The results show that in the distal element, D4 motif (-4073 to -4051 bp) is essential for vnd expression, and in the proximal element, both 660 bp from the 5` end and 172 bp from the 3` end are indispensable for vnd expression after embryonic stages. These results suggest that vnd may function throughout Drosophila life span beyond embryogenesis.

Foreign gene induction into Bombyx mori egg by electroporation

Min-Uk Kang,Si-Kab Nho,Seok-Woo Kang,Kwang-Ho Choi,Tae-Won Goo 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Electroporation is well known today as a powerful transfection technique and is useful for the study of gene expression. The advantage of the electroporation method is that large quantity of silkworm (Bombyx mori) eggs can be transformed in a very short time. However, how to use it for introducing foreign gene into silkworm eggs needs systematical investigation. In our silkworm transgenesis program, we needed an efficient technique to evaluate the functionality of transgenes before their injection into eggs. The goal of this experiment was to find an alternative efficient method of generating transgenic silkworm eggs using a commercially available electroporation device. The Gene Pulser Xcell (Bio-Rad Laboratories, USA) were used. In contrast to other electroporation devices, which are based on a single pulse with exponential decay or square wave technology. We investigated pigmentation-rate and hatching-rate of the silkworm eggs of electroporation. We used foreign gene LacZ, EGFP, Ds-red induced vector system with selection marker for transgenic silkworm. The LacZ gene in 3rd instar larva DNA can be detected by β-galactosidase stain. During these technical studies we found that optimizing parameters such as electrical voltage, number of pulses and their frequency, and conductivity of the buffer was important. These results confirmed that electroporation is available technique for transfecting B. mori egg.

십이지장주위탈장의 임상적 경험

우시욱(Si Uk Woo),윤대근(Dae Kun Yoon),강희준(Hui Jun Kang),전성은(Seong Eun Chon),박성길(Sung Gil Park),이재정(Jae Jung Lee) 대한외과학회 2004 Annals of Surgical Treatment and Research Vol.66 No.4

화상 사망환자 112명에 대한 임상적 고찰

우시욱 ( Si Uk Woo ),최규성 ( Gyu Sung Choi ),김도헌 ( Do Hern Kim ),허준 ( Jun Hur ),전욱 ( Wook Chun ),이재정 ( Jae Jung Lee ) 대한외상학회 2003 大韓外傷學會誌 Vol.16 No.2

Background: During the past several decades, advances in burn treatment, including the improvement in fluid ressusitation, the evolvement of antibiotics, and the early excision of the wound, have increased the survival rate of burn patients. How ever sepsis and organ dysfunction are major causes of death after burn trauma. This study focused on finding the demography of burn mortality and the cause of death and on characterizing the time course of severe burn injury. Method: From January 1, 2002, to December 31, 2002, 543 patients were treated at the Burn Unit of General Surgery, Han Gang Sacred Heart Hospital after burn trauma. Among them, 112 patients expired. We reviewed their medical records and analyzed their demography, the cause of death, and the survival period, retrospectively. Results: Among the 112 patient, the cases of death are most common in the male patients aged 40 to 49. The most common cause of burn was flame (94.6%). They expired from sepsis (63.39%), pulmonary complication (13.39%), burn shock (13.39%) and acute renal failure (6.25%). Unill 72 hours after the burn injury, burn shock was the most common cause of death, between the 3rd day and the 7th day, pulmonary complications were the most common cause and after the 7th day, sepsis was. Conclusions: Even nowadays, many burn patients expire from burn shock and sepsis. Rapid transportation to a specialized burn center, judicious fluid ressusitation, wound care for wound healing, and prophylaxis of secondary infection can save more massive-burn victims.

한국의 베트남에 대한 분야별 산업 ODA 전략

이시욱(Si-Uk Lee),최석준,최용석 산업연구원 2017 연구보고서 Vol.- No.-

BIM 기반 배근상세설계 자동화

김시욱(Kim, Si Uk),김치경(Kim, Chee Kyeong) 한국콘크리트학회 2017 한국콘크리트학회 학술대회 논문집 Vol.29 No.1