Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

페야쿠르트병을 이용한 오수정화시설의 유기물 처리 특성 및 서식 미생물 분류

전진화,김희숙,허시선,민병윤 경남대학교 환경문제연구소 1995 환경연구 Vol.17 No.-

The average concentration of BOD, SS, TKN and T-P of influent were 94.81, 105.58, 9.72㎎/L, respectively. Also, these average concentration of effluent were 7.94, 2.98, 18.21 and 4.95㎎/L, respectively. The corresponding removal efficiencies were 91.33% in BOD, 97.18% in SS, 67.30% in TKN and 49.0% in T-P. The process of this system was maintained stably because of flowing by gravity. The media used for this study was Yakult filter with specific surface area of 130㎡/㎥ and surface loading rate of 28㎡/㎥. In this system, the removal efficiencies of SS and sludge were higher than those of activated sludge process. Twenty eight senora and sixty eight species of aerobic bacteria were identified from floating water and Yakult filters in the septic tank. Especially, only two strains of the bacteria were found from the effluent. Alcaligenes, Corynebacterium, Flavobacterium, Pseudomonas, and Staphylococcus of the bacteria were found, and they were known as excellent bacteria for the degradation of organic matter. Also, Alcaligenes, Bacillus, Flavobacterium, Micrococcus, Proteus and Pseudomonas, which can denitrification organic matter, were found and phosphorus removal bacteria, Acinetobacter was identified.

UV-TiO_2 광촉매 반응기를 이용한 미생물의 살균효과

김시욱,이정섭,정혜광,박열,윤성명,유진철,이범규,이인화,박진열 조선대학교 환경연구소 1998 環境硏究 Vol.14 No.1

UV-TiO_2 반응기를 이용하여 Escherichia coli와 Aspergillus oryzae var. oryzae의 살균효과를 측정하였다. 254㎚에서 최대 14 watt의 자외선 방출량을 내는 램프를 원형 Pyrex유리관 중앙에 설치하였고 TiO_2는 석영관에 박막증착(Thin Film Coating)된 형태와 슬러리 형태로 나누어 회분식으로 살균정도를 측정하였다. E. coli에 대한 살균력은 1.7×10^7 cells/㎖에 대해 5분간 자외선 조사를 하였을 경우 2.0×10^2 cells/㎖으로 감소하였고, 자외선 조사와 함께 슬러리 형태의 TiO_2를 첨가하였을 경우에는 3.4×10 cells/㎖으로, 자외선 조사와 함께 TiO_2가 석영관에 박막증착된 경우에는 7.6×10^2 cells/㎖으로 감소하였다. 한편 위와 같은 조건에 유리관 하부에서 기포를 주입수 11분 동안 자외선을 조사시킨 경우에는 1.3×10^2 cells/㎖으로, 자외선 조사와 함께 슬러리 형태의 TiO_2를 첨가하였을 경우에는 1×102 cells/㎖으로, 자외선 조사와 함께 TiO_2가 박막증착된 경우에는 7.9×10 cells/㎖을 나타내었다. 결국 UV-TiO_2 반응기에 사용되는 TiO_2가 슬러리 형태일 때 최대 살균효과를 나타내었으나 기포가 첨가되었을때는 오히려 살균에 장애를 받는 것으로 나타난 반면 석영관에 박막증착된 경우에는 기포가 첨가되는 것이 살균에 효과적인 것으로 관찰되었다. The killing effect of UV-TiO_2 photocatalytic system on the Escherichia coli DH5-α and Aspergillus oryzae var. oryzae (KCTC 6095) was studied. The UV lamp which emits maximum 14 watts at 254 ㎚ was set on the center of pyrex round glass tube. Two types of TiO_2, one of which is slurry and another which is thin film coated form, were used to determine the killing effect. When UV was irradiated to 1.7×10^5 cells/㎖ of E. coli for 11 min, the living cell number decreased to 4.0×10^0 cells/㎖. The effect of UV system together with slurried TiO_2 was less than 1 cells/㎖, whereas that of UV-coated TiO_2 system decreased to 7.1×10^3 cells/㎖. To study the effect of bubble on the killing of microorganisms, air was bubbled to the bottom of glass tube. When 1.7×10^5 cells/㎖ were exposed to UV for 11 min in combination with air bubble, the living cell number decreased to 1.3×10^2 cells/㎖. In the same condition except the addition of slurried TiO_2, the living cells were 1×10^2 cells/㎖. However, more cells could be killed by the system which consists of UV, coated TiO_2, and air bubble (7.9×10^1 cells/㎖). From these results, it was found that UV-slurried system is the most effective one, but its killing effect is not stimulated by air bubble. However, bubbling was very effective in the UV-coated TiO_2 system.

生態指向的 環境敎育을 위한 中等學校 環境敎科書 分析

이인화,김시욱,조은주 조선대학교 환경공해연구소 1999 環境公害硏究 Vol.15 No.2

A analysis of environmental text book for secondary education has been conducted toward ecocentric education, eventually to provide a creative and open-minded teaching. Based on the result of analysis, the following problems suggested, the content of the text book has too wide scope for the intelligence of the middle school student. Furthermore, the text book is mainly focused on demonstrating the surface of the phenomena as an existing public nuisance in society, however, the present book deals with the superficial aspects of environmental problems and makes the problems considered as simple. From the analysis study of present environmental text book several suggestion has been conducted as follows : First, additional education units are needed for judgement traning toward emotional approach to law of nature and environmental phenomena. Second, it is necessary for teachers to develop various teaching methods in an environmental class.

화주 직접운항 선대의 운영 최적화 분석

김시화,이경근 한국경영과학회 1998 韓國經營科學會誌 Vol.23 No.4

The Industrial operation is one of the three basic modes of shipping operation with Liner and Tramp operations. Industrial operators usually control vessels of their own or on a time charter to minimize the cost of shipping their cargoes. Such operations abound in shipping of bulk commodities, such as oil, chemicals and ores. This work is concerned with an operational optimization analysis of the fleet owned by a major oil company. a typical Industrial operator. The operational optimization problem of the fleet of a major oil company is divided into two phase problem. The front end corresponds to the optimization problem of the transportation of crude oil. product mix. and the optimization problem of the fleet to meet the seaborne transportation demand derived from the front end. A case study reflecting the practices of an international major oil company is demonstrated to make clear the underlving ideas.

UV-TiO₂광촉매 반응기를 이용한 미생물의 살균효과

김시욱,이정섭,정해광,박열,윤성명,유진철,이범규,이인화,박진열 조선대학교 환경공해연구소 1998 環境公害硏究 Vol.14 No.-

UV-TiO_(2) 반응기를 이용하여 Escherichia coli와 Aspergillus oryzae var. oryzae의 살균효과를 측정하였다. 254 ㎚에서 최대 14 watt의 자외선 방출량을 내는 램프를 원형 Pyrex유리관 중앙에 설치하였고 TiO_(2)는 석영관에 박막증착(Thin Film Coating)된 형태와 슬러리 형태로 나누어 회분식으로 살균정도를 측정하였다. E. coli에 대한 살균력은 1.7× 10^(7) cells/㎖에 대해 5분간 자외선 조사를 하였을 경우 2.0× 10^(2) cells/㎖으로 감소하였고, 자외선 조사와 함께 슬러리 형태의 TiO_(2)를 첨가하였을 경우에는 3.4× 10 cells/㎖으로, 자외선 조사와 함께 TiO_(2)가 석영관에 박막증착된 경우에는 7.6× 10^(2) cells/㎖으로 감소하였다. 한편 위와 같은 조건에 유리관 하부에서 기포를 주입후 11분 동안 자외선을 조사시킨 경우에는 1.3× 10^(2) cells/㎖으로, 자외선 조사와 함께 슬러리 형태의 TiO_(2)를 첨가하였을 경우에는 1× 100 cells/㎖으로, 자외선 조사와 함께 TiO_(2)가 박막증착된 경우에는 7.9× 10 cells/㎖을 나타내었다. 결국 UV-TiO_(2) 반응기에 사용되는 TiO_(2)가 슬러리 형태일 때 최대 살균효과를 나타내었으나 기포가 첨가되었을때는 오히려 살균에 장애를 받는 것으로 나타난 반면 석영관에 박막증착된 경우에는 기포가 첨가되는 것이 살균에 효과적인 것으로 관찰되었다. The killing effect of UV-TiO_(2) photocatalytic system on the Escherichia coli DH5-慣 and Aspergillus oryzae var. oryzae (KCTC 6095) was studied. The UV lamp which emits maximum 14 watts at 254 nm was set on the center of pyrex round glass tube. Two types of TiO_(2), one of which is slurry and another which is thin film coated form, were used to determine the killing effect. When UV was irradiated to 1.7 * 10^(5) cells/??of E. coli for 11 min, the living cell number decreased to 4.0 * 1.0^(0) cells/?? The effect of UV system together with slurried TiO_(2) was less than 1 cells/?? whereas that of UV-coated TiO_(2) system decreased to 7.1 * 10^(3) cells/?? To study the effect of bubble on the killing of microorganisms, air was bubbled to the bottom of glass tube. When 1.7 * 10^(5) cells/??were exposed to UV for 11 min in combination with air bubble, the living cell number decreased to 1.3 x 10^(2) cells/?? In the same condition except the addition of slurried TiO_(2), the living cells were 1 * 10^(2) cells/?? However, more cells could be killed by the system which consists of UV, coated TiO_(2), and air bubble (7.9 * 10^(1) cells/??. From these results, it was found that UV-slurried system is the most effective one, but its killing effect is not stimulated by air bubble. However, bubbling was very effective in the UV-coated TiO_(2) system.

신사구체 경변증을 나타내는 FGS/NgaKist mice 간장에서 사이토크롬 P4501A1/2 단백질 발현

정규식,현병화,이철호,박상준,최양규,류시윤,이차수,민병길 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.2

대관령의 일조시간을 이용한 월별 일사량의 추정

박진기, 오시영, 백신철, 김봉섭, 신형섭, 박종화 忠北大學校 農業科學硏究所 2013 農業科學硏究 Vol.29 No.1

The monthly mean daily global solar radiation and sunshine duration, have been employed in this study to develop correlation model for Daegwallyeong during 2011. The corresponding monthly value of extraterrestrial solar radiation and possible duration of sunshine are calculated for Daegwallyeong meteorological data. Forecasting performance parameters such as coefficient of determination (R2), mean bias error (MBE) and root mean square error (RMSE) are presented as analyzed for the model. The model with multi-linear regression has the highest R2 value of 0.95 and average MBE and RMSE values of −0.11 MJ/m2day and 1.63 MJ/m2day, respectively. The model exhibited negative MBE which shows that the model resulted in underestimation of the global solar radiation relative to the meteorological measured value. The equation could be employed in the estimation of solar radiation at Daegwallyeong, other locations with similar meteorological characteristics.

토양 미생물인 Streptomyces tubercidicus에서 분리한 GTPcyclohydrolase Ⅰ 저해제

한지만,김시욱,이인화,박열,정혜광,윤성명,유진철 조선대학교 약학연구소 1998 藥學硏究誌 Vol.19 No.1

GTP cyclohydrolase I (EC 3.5.4.16) catalyzes the conversion of GTP to D-erythro-7,8-dihydroneopterin triphosphates and formic acid. and thus performs the first committing step in the biosynthesis of the pteridine moiety of folk acid in microorganisms and of tetrahydrobiopterin in higher animals. GTP cyclohydrolase I isolated from Streptomyces tubercidicus was inhibited by DL-6-methyl-5,6,7,8-tetrahydropterin and xanthopterin, and was not inhibited by tetrahydrofolic acid, pterin, pterin-6-carboxylic acid, biopterin, neopterin, ribofravin. These results suggest that bacterial GTP cyclohydrolase I may be regulated by its metaboilic end product.

연쇄상구균을 실험감염시킨 넙치치어의 병리조직학적 관찰

조성환,류시윤,김현수,박배근,김덕환,손화영,윤원기,신창호 충남대학교 수의과대학 동물의과학연구소 1999 動物醫科學硏究誌 Vol.7 No.-

Pathological observations were examined after abdominal inoculation in 7∼8㎝ sized flounder (Paralichthys olivaceus) with 1X10^7 cfu/㎖ of Streptococcus faecalis. The infected flounder were clinically observed and weighed a fish body at 0, 5, 10, 15, 20, 25, 30 days post inoculation. In infected group, mortality were 70% and feed efficiency were 7.2%. In non-infected group, mortality were 10% and feed efficiency were 18.2%. Macroscopically, the significant lesions were characterized by protrusions and opacity of the eyes and ulcer of the caudal pecuncle. Histopathologically, the lesions were observed a degeneration. necrosis and inflammatory cells in muscle of the caudal peduncle and a degeneration and necrosis of the rod cells and detached retina from the black pigmented layer