고령자 급성 췌장염의 임상적 고찰

백용한,서정훈,송건훈,이진헌,송시영,정재복,강진경,박인서,이수진 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.3

고령자 급성 췌장염의 임상적 고찰

이진헌,강진경,박인서,송건훈,정재복,백용한,송시영,서정훈 대한소화기학회 1998 대한소화기학회지 Vol.32 No.3

Background/Aims: Inflammatory diseasas of the pancreas are not uncommon. The advanced age is one of the significant factors in assessing the severity of acute pancreatitis. It has been shown that the biliary tract disease is a more frequent cause of acute pancreatitis in the elderly than in the young. With the increased life span, the elderly are comprising a large proportion of the population. As a result, physicians are more often being faced with acute pancreatitis in the elderly. The purpose af this study was to evaluate clinical features of acute panereatitis in the elderly. Methods: A total of 188 patients with acute pancreatitis were reviewed. We investigated the differences of clinical characteristics between the elderly patients aged over 60 and the young patients aged under 60. Results: The sex distribution showed male preponderance in the young patients (M:F=2.4:1), but was nearly equal in the elderly patients (M:F=1.1:1). The most common cause of acute pancreatitis in the elderly was biliary tract disease (52.5%), while alcohol abuse was the most common cause in the young patients (38.8%), Local complications of acute pancreatitis, such as psuedocyst, necrosis, abscesses were not differently shown between the elderly (16.9%) and the young (20.2%) patients. Systemic complications including acute puhnonary failure occurred more frequently in the elderly patients than the young patients. The mortality rate of the elderly patients (5.1%) was not significantly different from that of the young patients (3.1%). Conclusions: To detect correctable underlying bihary tract diseases the elderly patients with acute pancreatitis should be completely investigated using endoscopic retrograde cholangiopancreatography (ERCP). In addition, the patient should be closely monitored for the development of the systemic complications during the disease process.

분화중인 흰쥐 콩팥의 요세관에서 nNOS의 발현

송지현,류시윤,김진,정주영 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.2

Nitric oxide는 콩팥에서 혈류의 조절, 사구체 혈액역동학 및 요세관의 운반에 중요한 역할을 하는 인자로서 최근 세포의 주기조절, 유사분열에도 관여하는 것으로 알려져 있다. 최근 연구결과에 의하면 발생중인 콩팥에서의 nNOS의 발현은 성체와는 다른 것으로 알려져 있으나, 발생중인 콩팥에서의 발현 양상 및 발현시기 등에 대해서 알려진 바 없다. 따라서 본 연구에서는 발생중인 콩팥을 이용하여 분화중인 요세관에서 nNOS의 발현양상을 관찰하고자 하였다. 성체에서 nNOS의 면역반응성은 사구체 보우만주머니의 벽측상피와 치밀반, 내림가는부분 및 수질집합관에 발현되었다. 발생중인 콩팥에서는 임신 15일에 처음 발현하기 시작하였으며, 그 부위는 원위세관의 굵은오름부분이었다. 임신 20일에서 출산후 14일까지 새로이 형성되는 요세관의 굵은오름부분에서 관찰되었으나, 분화를 마친 요세관에서는 그 발현 양상이 감소하였다. 출생후 21일경에는 최종적으로 치밀반에만 국한되어 발현되었다. 속수질에서는 내림가는 부분과 속수질 집합관에서 발현되었으며 그 양상은 수질 중간부위에서 강하게 발현되었으며 시작부위와 말단부위에서는 약하게 발현되었다. 이상의 결과로 보아 굵은오름부분에서의 nNOS의 발현은 요세관의 분화정도와 일치하며 특히 그 발현양상이 분화가 활발한 시기에 강하게 발현되고 분화가 저하된 후에는 발현이 감소하는 것으로 보아 발생중인 콩팥의 성장에 중요한 역할을 할 것으로 생각된다. Nitric oxide (NO) is an important regulator of renal blood flow, glomerular hemodynamics, and tubule transport processes in the kidney. There is also evidence that NO is involved in cell cycle regulation and mitotic division. During development the nNOS expression pattern differs from that observed in adult animals. However, little is known about temporal and spatial patterns of nNOS expression in the developing kidney. The purpose of this study was to establish the time of expression and the distribution of nNOS in the developing rat kidney. Kidneys from 14-, 16-, 17-, 18-, and 20-day-old fetuses, 1-, 4-, 7-, 14-, and 21-day-old pups, and adult animals were preserved and processed for immunohistochemistry. In the adult kidney, nNOS was detected in the parietal epithelium of Bowman’s capsule, macula densa, descending thin limb and inner medullary collecting duct. nNOS immunoreactivity appeared first in the distal tubule anlage at 15 days of gestation, and in all epithelial cells of developing thick ascending limbs (TAL) as well as macula densa of 17- and 18-day-old fetuses. From 20 days of gestation to 14 days after birth, nNOS was expressed in the newly formed cortical TAL, which are located in the medullary ray, whereas in mature TAL of juxtamedullary nephrons, nNOS immunolabeling gradually decreased in intensity and became restricted to the macula densa. In inner medullary collecting ducts, nNOS immunoreactivity appeared first at 7 days after birth in the papillary tip and gradually ascended to the border between outer and inner medulla. In the descending thin limb and parietal epithelium of Bowman’s capsule, weak nNOS immunoreactivity was observed at 14 days after birth and labeling gradually increased to adult levels at 21 days after birth. These results suggest that differential expression of nNOS in the developing kidney is an important physiological regulator of renal function during kidney maturation.

흉부 둔상 후 8년간 지속된 삼첨판폐쇄부전의 치료 1예

송현주,남승현,최영주,박성훈,박시훈,한재진 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.4

PWR 발전소에서의 방사선장 특성

송명재,김희근,김봉환,장시영 대한방사선 방어학회 1992 방사선방어학회지 Vol.17 No.2

국내 원전의 주종인 가압경수로를 대상으로 스펙트로메트리 기법에 의한 감마, 중성자 그리고 베타 방사선장애 대한 특성조사를 수행하였다. 감마선장의 경우, HPGe 반도체 검출기로 구성된 휴대용 감마 스펙트로메타를 사용하여 측정한 결과, 평균 에너지 값의 분포는 가동정지중일 때 원전의 격납용기내에서 440∼780keV, 가동중일 때 원전의 보조건물내에서 280∼760keV로 나타났다. 가동중인 원전의 격납용기내 operation deck를 중심으로 5개 지점에 대하여 BMSS(Bonner Multiple Spectrometry System)을 사용하여 중성자선장을 조사하였다. BUNKI 코드를 사용하여 측정스펙트럼을 분해하였으며, 측정 장소에서의 평균 에너지 값의 분포는 20∼210keV이었다. 베타피폭원인 오염에 대하여 가동정지 상태인 원전의 격납용기내와 보조건물의 총 14개 지점의 고 방사성 오염지역에서 smearing 시료를 채취하여 표면장벽형 Si 반도체 검출기를 사용하여 에너지 스펙트럼을 측정하였다. 그 결과 부식생성물 60Co에 의한 베타 에너지가 지배적인 것으로 나타났다. Pheton, neutron and beta radiation fields were measurement at PWR plants which are the representative types of nuclear power plant operated in Korea. The photon energy spectra were measurement at locations in the auxiliary building during operation period and in the containment vessel(C/V) during shutdown period using a portable gamma spectrometer with a HPGe detector. The distribution of average energy was found to range from 440 to 780 keV in the C/V and from 280keV to 760keV in the auxiliary building respectively. The average neutron energy measured at the five locations round the operation deck in the C/V in operation using a BMSS(Bonn Multi-Sphere Spectrometer)ranged from 20 keV to 210keV. A computer code, BUNKI was used to unfold the spectrum. The beta energy spectra in the C/V asnd in the auxiliary building in annual outage were determined using 14 smear samples taken from the highly contaminated areas. The analysis showed that representative corrosion product, ??Co made main contribution to the beta energy field.

境界要素法에 의한 3次元 基礎一地盤 相互作用 시스템의 地震解析

權映錄,宋始榮,姜圓鎬,崔光圭,柳又秀 동아대학교 공과대학 부설 한국자원개발연구소 1990 硏究報告 Vol.14 No.1

In this study, the seismic analysis procedure of foundation-soil interaction system is presented. With the assumption of isotropic material properties and infinitesimal strain theory, the boundary element models of three dimensional foundation plate an semi-infinite soil medium are coupled together, using the compatiblity condition of displacements and equilibrium condition of surface tractions on the contact surface. Through the results of the examples in which the seismic responses of the foundation subjected to the SV-wave and the P-Wave, it is shown that the translational motions are decreased and the rocking motion is occured in higher frquency range.

내산성 및 내담즙성이 우수한 양돈용 유산균의 선발

유자영,양시용,송민동 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.1

본 연구는 가축의 장내에서 유익한 영향을 주는 유산균을 돼지의 분변으로부터 분리하고 내산성과 내담즙성이 뛰어난 균을 선별하여 유산균 생균체를 준비하고자 실시하였다. 분변에 섞여 있는 다른 균들과 산이나 담즙액에 약한 균들은 처음부터 분리하기 위하여 pH를 2.0으로 맞춘 MRS broth에 배양시키고 oxgall이 첨가된 배지에 도말하여 40개의 균주를 분리하였다. BCP배지를 이용해서 유산의 생성여부를 확인하고, O.D값과 pH를 측정하였으며, 그람염색과 형태관찰을 통하여 33개의 균주를 분리하였다. 분리된 균주중에서 산에 대한 내성이 있다고 판단되는 12개의 균주를 분리해냈고, 12개의 균주의 내산성 및 내담즙성을 3회에 걸쳐 시험하고 내산성과 내담즙성이 우수한 3개의 균주를 선별하였다. 마지막으로 담즙산 농도별 시험을 행함으로써 가축의 장까지 살아서 도달하기 위해 꼭 갖추어야 할 조건인 내산성과 내담즙성이 가장 좋은 #32-1 균주를 최종 선발하였다. 유산균이 생균제로서의 기능을 수해하려면 oxgall이 0.3% 함유된 배지에서 성장할 수 있을 정도의 내성을 갖고 있어야 한다는 보고가 있는데 #32-1 균주의 경우 담즙액의 농도가 3%인 배양액에서도 높은 생존률을 보임으로써 생균제로서 사용하기위해 갖고 있어야 할 담즙에 대한 내성이 우수하다고 판단하였다. This study was performed to screen lactic acid bacteria for the direct-fed-microbials. A lactic acid bacteria which has resistant to acid and bile salts were isolated from swine feces. Among the previously obtained acid tolerant strains, three of them show resistant to artifical gastric juice and bile. Finally, 321- stain was selected for good growth an high survival rate. The finally selected 32-1 stain was identified as Lactobacillus salivarius. The strain, which named L. salivarius JY 32-1, was able to tolerate acid condition down to pH 2.5 and could also tolerate bile at 3%.

중합효소연쇄반응 기법을 이용한 돼지 증식성 장염 진단 기법의 확립

김대영,권언혜,송민동,양시용 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.1

This study was carried out to establish a efficient diagnosis method of pocine proliferative enteropathy (PPE) contributing major economic loss by inducing weight loss, poor growth and/or sudden death through out the growth period(6~22 wk). Therefore, in this study we established the PCR (polymerase chain reaction) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L. intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L. intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect lng of genomic DNA as a template. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific dignistic tool for PPE.

가축 생균제용 유산균의 분리 및 동정

신진영,양시용,송민동 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.2

본 연구가 가축 생균제(direct-fed microbials)용 내산성 및 내담즙성이 우수한 유산균을 분리하기 위하여 실시하였다. 자돈 분변으로 분리한 유산균 중 혐기성 조건에서 성장이 우수한 8균주가 1차적으로 선발되었다. 선발 균주들 중 내산성 및 내담즙성이 우수한 JY-12 및 JY-73를 최종 선발하였다. 최종 선발 균주들을 형태학적 생리학적 특서에 따라 동정한 결과 Lactobacillus salivarius로 동정되었다. The objective of this study was isolation of acid and bile tolerant lactic acid bacteria for direct-fed microbials. Among lactic acid bacterial isolated from piglet feces, 8 strains having high anaerobic growth ability were selected. Among isolated strains, JY-12 and JY-73 strains were selected according to the acid and bile tolerant characteristics. Finally selected strain JY-12 and JY-73 were identified as Lactobacillus salivarius based on their morphological and biochemical characteristics.

주방환기 시스템의 공기 급기구의 위치에 따른 성능평가

임경빈,송시영,이찬호 한밭대학교 생산기반기술연구소 2003 생산기반기술연구소 논문집 Vol.3 No.1

The purpose of this study is to investigate the performance evaluation of indoor diffusion rate of surplus heat generated from gas range and concentration distribution of combustion gas by position change of air- diffuser and range-hood with separating plate. A numerical analysis is performed with four cases of this ventilation system for kitchen. The results showed that thermal and flow distribution can be more effective, when air supply diffuser is located at middle position. Also, Comparisons of Indoor Air Quality(IAQ) are made between using separating plate and not using, IAQ was improved in case of using separating plate and also, kitchen exhaust hood concentration distribution was improved about 30%