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( Shunpeng Song ),( Peter M Elias ),( Theodora M Mauro ),( Mao Qiang Man ) 한국피부장벽학회 2013 한국피부장벽학회지 Vol.15 No.2
Aged skin is featured by compromised epidermal permeability barrier homeostasis. Improvement of epidermal permeability barrier in aged skin is quite a challenge. Previous study demonstrated that topical hesperidin improves epidermal permeability barrier homeostasis in young mice. However, whether topical applications of hesperidin benefit epidermal permeability barrier function is not known yet. In the present study, we assessed the influence of topical applications of hesperidin on epidermal permeability barrier function and its underlying mechanisms in aged murine model. 12-15 month old hairless mice were topically treated with either 2% hesperidin or 70% ethanol alone twice daily for 25 days. At the end of study, stratum corneum function was assessed with an MPA5 physiology monitor. And barrier recovery rates were determined at 2 and 4 hours after barrier disruption by repeated tape-stripping. Our results show that topical applications of hesperidin lowered basal skin surface pH and increased basal transepidermal water loss. However, barrier recovery was significantly accelerated by hesperidin. The improved barrier function correlated with up-regulation of barrier-related protein, lipid synthetic enzyme and their mRNA expression. Moreover, topical hesperidin also significantly increased expression of epidermal mRNAs for mBD3, NHE1 and sPLA2g2f. These results suggest that topical application of hesperidin could be useful for preventing skin aging and treating aged skin.
Chunxian Tao,Zhaoxia Han,Jun Ruan,Shunpeng Shu,Zhongrong Lu,Ruijin Hong,Dawei Zhang 한국광학회 2017 Current Optics and Photonics Vol.1 No.4
In order to investigate the ultraviolet-excited photoluminescence properties of phosphor coatings andtheir relationship to thickness, Lumogen coatings with different thicknesses were deposited on quartzsubstrates and charge-coupled device chips by thermal evaporation. The variation of the film thicknessaffected the crystallite size, surface roughness and fluorescence signal. It was found that the Lumogencoating with the thickness of 420 nm has the largest luminescent signal and conversion efficiency, andthe corresponding coated charge-coupled devices had the maximum quantum efficiency in the ultraviolet. These results provided one key parameter for improving the sensitivity of Lumogen coated charge-coupleddevices to ultraviolet light.
Analysis of nifH Gene Diversity in Red Soil Amended with Manure in Jiangxi, South China
Qihui Teng,Bo Sun,Xinrui Fu,Shunpeng Li,Zhongli Cui,Hui Cao 한국미생물학회 2009 The journal of microbiology Vol.47 No.2
In order to understand the community structure of diazotrophs in red soil and effects of organic manure application on the structure, four nifH gene libraries were constructed: the control (CK), low manure (LM), high manure (HM), and high manure adding lime (ML). Totally 150 nifH gene clones were screened and grouped into 21 clusters by RFLP analysis. Existence of dominant patterns was observed in all libraries, which counted for over 96% of clones in library HM and about 56~72% in other three libraries. The nifH sequences of the dominant patterns in all libraries were most similar to sequences of the cyanobacteria. nifH genes showed high diversity in red soil, dispersing throughout the nifH clades (alpha-, beta-, and gamma- Proteobacteria, Firmicutes, cyanobacteria, Verrucomicrobia, and posited group). Bradyrhizobium and Burkholderia were also important diazotrophs in low fertility soil samples. Low manure treatment increased the diversity of nifH genes compared with CK and high manure treatments. Manure and lime treatment led to obvious community succession. Total N to available P ratio, total carbon, and K concentrations were the main factors affecting the diversity of diazotrophs in red soil.
Tang Ri,Tang Ri,Xu Qiaoyi,Feng Jinhua,Zhou Yang,Xing Shunpeng,He Zhengyu,Gao Yuan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Recent clinical research has revealed that mechanical ventilation (MV) can initiate pulmonary fibrosis and induce mechanical ventilation-induced pulmonary fibrosis (MVPF). However, the underlying mechanism remains largely uncharacterized. Based on a mouse model of MVPF and an alveolar epithelial cell cyclic strain model, the present study explores the possible mechanism of MVPF. Single-cell RNA-sequencing and EV RNA-sequencing analysis revealed that MV promoted apoptosis signal-regulating kinase 1 (ASK1)-mediated endoplasmic reticulum (ER) stress pathway activation and extracellular vesicle (EV) release from alveolar epithelial cells. Furthermore, the ASK1-ER stress pathway was shown to mediate mechanical stretch (MS)- or MV-induced EV release and lung fibroblast activation in vivo and in vitro. These processes were suppressed by ER stress inhibitors or by silencing ASK1 with ASK1- short hairpin RNA (shRNA). In addition, MVPF was suppressed by inhibiting ASK1 and ER stress in vivo. Therefore, the present study demonstrates that ASK1-ER stress pathway-mediated fibrotic-EV release from alveolar epithelial cells contributes to fibroblast activation and the initiation of pulmonary fibrosis during MV. The inhibited release of EVs targeting the ASK1-ER stress pathway might be a promising treatment strategy for MVPF.