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A Simple Colorimetric Method for Testing Antimicrobial Susceptibility of Biofilmed Bacteria
Shukho Kim,김미진,Hee Young Kang,설성용,조동택,김정민 한국미생물학회 2010 The journal of microbiology Vol.48 No.5
This study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (TTC) as an indicator of viable bacteria. The new method was utilized for the evaluation of antibiotic susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus biofilms.
Inverse PCR for subtyping of Acinetobacter baumannii carrying ISAba1
Shukho Kim,Yun-Ju Park,Jungmin Kim 한국미생물학회 2016 The journal of microbiology Vol.54 No.5
Acinetobacter baumannii has been prevalent in nosocomial infections, often causing outbreaks in intensive care units. ISAba1 is an insertion sequence that has been identified only in A. baumannii and its copy number varies among strains. It has been reported that ISAba1 provides a promoter for blaOXA-51-like, blaOXA-23-like, and blaampC, which are associated with the resistance of A. baumannii to carbapenems and cephalosporins. The main purpose of this study was to develop a novel inverse PCR method capable of typing A. baumannii strains. The method involves three major steps: cutting of genomic DNA with a restriction enzyme, ligation, and PCR. In the first step, bacterial genomic DNA was digested with DpnI. In the second step, the digested genomic DNAs were ligated to form intramolecular circular DNAs. In the last step, the ligated circular DNAs were amplified by PCR with primers specific for ISAba1 and the amplified PCR products were electrophoresed. Twenty-two clinical isolates of A. baumannii were used for the evaluation of the inverse PCR (iPCR) typing method. Dendrogram analysis revealed two major clusters, similar to pulsed-field gel electrophoresis (PFGE) results. Three ISAba1-associated genes – blaampC, blaOXA-66-like, and csuD – were amplified and detected in the clinical isolates. This novel iPCR typing method is comparable to PFGE in its ability to discriminate A. baumannii strains, and is a promising molecular epidemiological tool for investigating A. baumannii carrying ISAba1.
Shukho Kim,Sung Guen Chun,Ok Young Lim,Mi Sun Park,Yeon Ho Kang,박용호,이복권 한국미생물학회 2004 The journal of microbiology Vol.42 No.1
Salmonella enterica serovar Typhimurium DT104 (Salmonella Typhimurium DT104 or DT104) has been emerging as a common pathogen for human in Korea since 1997. In order to compare the genomic relationship and to search for the dominant strains in Korea, we conducted pulsed-field gel electrophoresis (PFGE) and IS200 fingerprinting of 25 epidemiological unrelated isolates from human and animals from Korea and cattle from America. Two Salmonella Typhimurium DT104 isolates from human in Korea and all 8 isolates from American cattle had indistinguishable patterns from the PFGE and IS200 fingerprinting but multidrug-resistant Salmonella Typhimurium isolates, including DT104, from Korean animals had diverse genetic patterns. The data suggest that a dominant DT104 strain might have circulated between Korean and American cattle and that it had a high level of clonality.
Kim, Shukho,Rahman, Marzia,Seol, Sung Yong,Yoon, Sang Sun,Kim, Jungmin American Society for Microbiology 2012 Applied and environmental microbiology Vol.78 No.17
<B>ABSTRACT</B><P>We isolated a new lyticPseudomonas aeruginosaphage that requires type IV pili for infection. PA1Ø has a broad bactericidal spectrum, covering Gram-positive and Gram-negative bacteria, and can eradicate biofilm cells. PA1Ø may be developed as a therapeutic agent for biofilm-related mixed infections withP. aeruginosaandStaphylococcus aureus.</P>
KIM, SHUKHO,LIM, OK YOUNG,KIM, SEONG HAN,KIM, JUN YOUNG,KANG, YEON HO,LEE, BOK KWON 한국미생물 · 생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.1
In early 2002, over 200 people in the city of Pusan. Korea suffered from paratyphoid fever resulting from Salmonella Paratyphi A infection. Antimicrobial susceptibility tests and Xbal pulsed-field gel electrophoresis (PEGE) were conducted to 54 Salmonella Paratyphi A isolated from humans during the period of 1998 to 2002. Most of the isolated (83%) were only nalidixic acid-resistant and 78% were X 1 PEGE patterns. Also, we measured the MIC of ciprofloxacin and screened gyrA mutation(s) using allele-specific PCR and restriction fragment length polymorphism (AS-PCR-RFLP). The representative 5 isolates in 2002 and 1 isolate in 2000 were 1 ㎍/ml of MIC and had mutation at the 83rd codon in gyrA. These data suggest that the outbreak in the early 2002 might have been due to dissemination of the strain present in 2000. Also, decreased susceptibility to ciprofloxacin was partly due to the mutation at the 83rd codon in gyrA.