Rapid screening and identification of metabolites of quercitrin produced by the human intestinal bacteria using ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry

Shu Jiang,Jing Yang,Dawei Qian,Jianming Guo,Er-xin Shang,Jin-ao Duan,Jun Xu 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.2

Ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF MS)technique combined with MetabolynxTM software was usedfor analysis of the metabolites of quercitrin by the isolatedhuman intestinal bacteria from the human feces. Four metabolitesof quercitrin were detected and tentatively identifiedbased on the characteristics of their protonated ions. Themetabolites were metabolized by four main metabolic pathwaysincluding hydroxylation, demethylation, deglycosylationand ring-cleavage. Quercitrin was metabolized to the hydroxyquercitrinand desmethylquercitrin by themajority of theisolated intestinal bacteria such as Bacteroides sp. 54, and wasdegraded to the deglycosylated product quercetin by rhamnosidaseand further ring-cleavage metabolite 3,4-dihydroxybenzoicacid by the minority of the isolated bacteria such asBacteroides sp. 45. The metabolic pathways and most of themetabolites of quercitrin were reported for the first time.

Screening of Endophytic Antagonistic Bacterium from Phellodendron amurense and Their Biocontrol Effects against Canker Rot

Li, Shujiang,Fang, Xinmei,Zhang, Hanlian,Zeng, Yanling,Zhu, Tianhui The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.3

Thirty-four strains of bacteria were isolated from Phellodendron amurense. Using Nectria haematococca as an indicator strain, the best strain, B18, was obtained by the growth rate method. The morphological, physiological and biochemical characteristics of strain B18 and its 16S DNA gene sequence were identified, and the biocontrol effect of strain B18 was assessed in pot and field tests, as well as in a field-control test. Drilling methods were used to determine the antibacterial activity of metabolites from strain B18 and their effects on the growth of pathogen mycelia and spores. The best bacteriostatic rate was 85.4%. B18 can hydrolyse starch and oxidize glucose but does not produce gas; a positive result was obtained in a gelatine liquefaction test. According to 16S DNA gene sequencing, strain B18 is Bacillus methylotrophicus (GenBank accession number: MG457759). The results of pot and field-control trials showed 98% disease control when inoculating $10^8cfu/ml$ of the strain. The disease control effect of the B18 culture liquid (concentrations of $10^8$, $2{\times}10^6$, $10^6$, $5{\times}10^5$ and $2.5{\times}10^5cfu/ml$) in the field-control test was higher than 80%, and the cure rate of the original delivery solution was 96%. Therefore, in the practical forestry production, a $2.5{\times}10^5cfu/ml$ culture liquidshould be applied in advance to achieve good control effects.

Interval Observer-based Output Feedback Control for a Class of Interconnected Systems with Uncertain Interconnections

Zhi-Hui Zhang,Shujiang Li,Hua Yan 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.4

This paper studies the output feedback controller design problem for a class of interconnected systemswith uncertain interconnections. First, the interval observers are built to guarantee the interval property betweenthe system states and their estimations. Then, the state feedback controller is designed for each observer systemother than output feedback controller for original system. A cyclic-small-gain condition is used to deal with thebound functions of the uncertain interconnections. The original closed-loop system is robust stable since that theinterval properties are satisfied. The non-convex conditions caused by traditional observer-based output feedbackare avoided by transforming the output to state feedback problem. Finally, the main results are demonstrated bynumerical simulations.

Preparation of Nanomaterial Wettable Powder Formulations of Antagonistic Bacteria from Phellodendron chinense and the Biological Control of Brown Leaf Spot Disease

Yanling Zeng,Han Liu,Tianhui Zhu,Shan Han,Shujiang Li 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.3

Brown leaf spot disease caused by Nigrospora guilin- ensis on Phellodendron chinense occurs in a large area in Dayi County, Chengdu City, Sichuan Province, China each year. This outbreak has severely reduced the production of Chinese medicinal plants P. chinense and caused substantial economic losses. The bacterial isolate JKB05 was isolated from the healthy leaves of P. chinense, exhibited antagonistic effects against N. guilinensis and was identified as Bacillus megaterium. The following fermentation medium and conditions improved the inhibitory effect of B. megaterium JKB05 on N. guilinensis: 2% glucose, 0.1% soybean powder, 0.1% KCl, and 0.05% MgSO4; initial concentration 6 × 106 cfu/ml, and a 42-h optimal fermentation time. A composite of 0.1% nano-SiO2 JKB05 improved the thermal stability, acid-base stability and ultraviolet resistance by 16%, 12%, and 38.9%, respectively, and nano-SiO2 was added to the fermentation process. The best formula for the wettable powder was 35% kaolin, 4% polyethylene glycol, 8% Tween, and 2% humic acid. The following quality test results for the wettable powder were obtained: wetting time 87.0 s, suspension rate 80.33%, frequency of microbial contamination 0.08%, pH 7.2, fineness 95.8%, drying loss 1.47%, and storage stability ≥83.5%. A pot experiment revealed that the ability of JKB05 to prevent fungal infections on P. chinense increased considerably and achieved levels of control as high as 94%. The use of nanomaterials significantly improved the ability of biocontrol bacteria to control this disease.

Complete mitochondrial genome of the Pleuronichthys lighti (Pleuronectiformes, Pleuronectidae) with phylogenetic consideration

Yaoxuan Song,Tianxiang Gao,Na Song,Yongdong Zhou,Pengfei Li,Shujiang Zhao 한국유전학회 2017 Genes & Genomics Vol.39 No.11

Mitochondrial genome has been used to shed light on many fields of both basic and applied research, including the study of molecular evolution. The complete mitochondrial genome sequence of 17368 bp nucleotides from the Pleuronichthys lighti was determined. It was a circular double-stranded DNA molecule with identical set of 22 transfer RNA genes, 2 ribosomal RNA genes, 13 proteincoding genes as well as a non-coding control region. Stand asymmetry in the nucleotide composition was reflected in the codon usage of genes oriented in opposite directions. In the control region, we identified the extended termination associated sequence domain, the central conserved sequence block domain and the conserved sequence block domain, and two complete repeat region. They were “TTA CAA TA” and “TGT TGT AA”, respectively. All known 12 mitochondrial genomes of Pleuronectinae fishes were downloaded and analyzed; there were 5570 variable sites in the consensus sequences of 15241 base pairs, calculation of total sites were 35.5%. The highest sequence divergence was 50% (ATP8) and the Kimura-2-parameter genetic distance was 0.235 (ND6), whereas the COIII had the lowest sequence divergence (28.8%) and genetic distance (0.128); the protein coding genes were mainly acted by purifying selection which was detected by selection tests. Analysis of confidence and the information content for per nucleotide revealed ND5, ATP6, COI and ND4 genes were suitable molecular markers for phylogenetic study of Pleuronectinae fishes. Phylogenetic analysis using Bayesian computational algorithms based on COI genes provided support for the taxonomic status of P. lighti, which was consistent with the traditional taxonomy.

Construction of hybrid bowl-like structures by anchoring NiO nanosheets on flat carbon hollow particles with enhanced lithium storage properties

Liang, Jin,Hu, Han,Park, Hyunjung,Xiao, Chunhui,Ding, Shujiang,Paik, Ungyu,Lou, Xiong Wen (David) The Royal Society of Chemistry 2015 ENERGY AND ENVIRONMENTAL SCIENCE Vol.8 No.6

<P>Bowl-like hybrid structures have been designed and fabricated by anchoring NiO nanosheets on flat carbon hollow particles. When evaluated as an anode material for lithium-ion batteries, these unique NiO/carbon hybrid particles exhibit superior lithium storage properties in terms of high capacity, long term cycling stability and excellent rate capability.</P> <P>Graphic Abstract</P><P>Bowl-like hybrid structures constructed by anchoring NiO nanosheets on flat carbon shells exhibit superior lithium storage properties. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5ee01125f'> </P>

Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa

Guoliang Li,Shifan Zhang,Fei Li,Hui Zhang,Shujiang Zhang,Jianjun Zhao,Rifei Sun 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.1

Plants protect against viruses through passive and ac- tive resistance mechanisms, and in most cases charac- terized thus far, natural recessive resistance to potyvi- ruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimo- lecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and trans- lation initiation factors to reveal the working of key amino acids.

Screening of Antagonistic Bacillus against Brown Rot in Dendrocalamus latiflorus and Preparation of Applying Bacterial Suspension

Fengying Luo,Hang Chen,Wenjian Wei,Han Liu,Youzhong Chen,Shujiang Li 한국식물병리학회 2024 Plant Pathology Journal Vol.40 No.1

The aim of this study was to isolate biocontrol bacteria that could antagonize brown rot of <i>Dendrocalamus latiflorus</i>, optimize the culture conditions, and develop an effective biocontrol preparation for brown rot of <i>D. latiflorus</i>. This study isolated a bacterium with an antagonistic effect on bamboo brown rot from healthy <i>D. latiflorus</i> rhizosphere soil. Morphology, molecular biology, and physiological biochemistry methods identified it as <i>Bacillus siamensis</i>. The following culturing media and conditions improved the inhibition effect of <i>B. siamensis</i>: the best culturing media were 2% sucrose, 1.5% yeast extract, and 0.7% potassium chloride; the optimal culturing time, temperature, pH, and inoculation amount were 48 h, 30℃, 6, and 20%. The optimum formula of the applying bacterial suspension was 14% sodium dodecyl benzene sulfonate emulsifier, 4% Na₂HPO₄·2H₂O, 0.3% hydroxypropyl methylcellulose thickener, and 20% <i>B. siamensis</i>. The pot experiment results showed the control effect of applying bacterial suspension, diluted 1,000 times is still better than that of 24% fenbuconazole suspension. The applying bacterial suspension enables reliable control of brown rot in <i>D. latiflorus</i>.