Establishment of Tripterygium wilfordii Hook. f. Hairy Root Culture and Optimization of Its Culture Conditions for the Production of Triptolide and Wilforine

( Chuan Shu Zhu ),( Guo Qeng Miao ),( Jia Guo ),( Yan Bo Huo ),( Xing Zhang ),( Jia Hua Xie ),( Jun Tao Feng ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.6

In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of the important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone-free half-strength Murashige and Skoog medium but also could produce sufficient amounts of both triptolide and wilforine. Although hairy roots produced approximately 15% less triptolide than adventitious roots and 10% less wilforine than naturally grown roots, they could grow fast and could be a suitable system for producing both secondary metabolites compared with other tissues. Addition of 50 μM methyl jasmonate (MeJA) could slightly affect hairy root growth, but dramatically stimulated the production of both triptolide and wilforine, whereas 50 μM salicylic acid had no apparent effect on hairy root growth with slightly stimulatory effects on the production of both secondary metabolites. Addition of precursor nicotinic acid, isoleucine, or aspartic acid at the concentration of 500 μM had varying effects on hairy root growth, but none of them had stimulatory effects on triptolide production, and only the former two had slightly beneficial effects on wilforine production. The majority of triptolide produced was secreted into the medium, whereas most of the produced wilforine was retained inside of hairy roots. Our studies provide a promising way to produce triptolide and wilforine in T. wilfordii hairy root cultures combined with MeJA treatment.

Study on the engineering and electricity properties of cement mortar added with waste LCD glass and piezoelectric powders

Shu-Chuan Chang,Chien-Chih Wang,Her-Yung Wang 사단법인 한국계산역학회 2018 Computers and Concrete, An International Journal Vol.21 No.3

This study used a volumetric method for design. The control group used waste Liquid Crystal Displayplay (LCD) glass powder to replace cement (0%, 10%, 20%, 30%), and the PZT group used Pd-Zr-Ti piezoelectric (PZT) powder to replace 5% of the fine aggregate to make cement mortar. The engineering and the mechanical and electricity properties were tested; flow, compressive strength, ultrasonic pulse velocity (UPV), water absorption and resistivity (SSD and OD electricity at 50 V and 100 V) were determined; and the correlations were determined by linear regression. The compressive strength of the control group (29.5-31.8 MPa) was higher than that of the PZT group (25.1-29 MPa) by 2.8-4.4 MPa at the curing age of 28 days. A 20% waste LCD glass powder replacement (31.8 MPa) can fill up finer pores and accelerate hydration. The control group had a higher 50 V-SSD resistivity (1870-3244 Ω.cm), and the PZT group had a lower resistivity (1419-3013 Ω.cm), meaning that the resistivity increases with the replacement of waste LCD glass powder. This is because the waste LCD glass powder contains 62% SiO2, which is a low dielectric material that is an insulator. Therefore, the resistivity increases with the SiO2 content.

Understanding consumers’ responses toward social media advertising and purchase intention toward luxury products

Shu-Chuan Chu,Sara Kamal,Yoo Jung Kim 한국마케팅과학회 2013 Journal of Global Fashion Marketing Vol.4 No.3

Social media has rapidly risen in popularity as a new advertising platform that allowsusers to connect with one another and engage with brands. At the same time, the onlineluxury market has experienced rapid expansion due to the rising number of affluentonline users between 18 and 35 years old. The current study examined young socialmedia users’ beliefs, attitudes, and behavioral responses toward social mediaadvertising. Brand consciousness was found to have an impact on users’ attitudestoward social media advertising, which in turn influences their behavioral responsestoward social media advertising and consequent purchase intentions toward luxuryproducts.

Re-examining of consumers’ responses toward social media advertising and purchase intention toward luxury products from 2013 to 2018: A retrospective commentary

Shu-Chuan Chu,Sara Kamal,Yoo Jung Kim 한국마케팅과학회 2019 Journal of Global Fashion Marketing Vol.10 No.1

The purpose of this retrospective commentary is to reexamine what we know about consumers’ response toward social media advertising and purchase intention toward luxury products, as well as review how researchers have cited this article. This commentary is organized as follows. First, it discusses the unique contributions of our article that causes its high impact in the literature. Second, it examines the topic of social media and luxury brands from today’s perspective. Third, we offer our interpretation of the use in the literature of our study and categorize the literature into three areas: luxury brand marketing and consumer engagement in social media; beliefs, attitudes, and behavioral responses toward social media advertising; and the state of knowledge on luxury brands and fashion advertising. Lastly, this commentary offers ideas in luxury brand advertising and marketing research and new trends in luxury brand digital strategies through social media.

Alpinate Oxyphyllae Fructus Inhibits IGFII-Related Signaling Pathway to Attenuate Ang II-Induced Pathological Hypertrophy in H9c2 Cardiomyoblasts

Chuan-Te Tsai,Yung-Ming Chang,Shu-Luan Lin,Yueh-Sheng Chen,Yu-Lan Yeh,Viswanadha Vijaya Padma,Chin-Chuan Tsai,Ray-Jade Chen,Tsung-Jung Ho,Chih-Yang Huang 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.3

Angiotensin II (Ang II) is a very important cardiovascular disease inducer and may cause cardiac pathological hypertrophy and remodeling. We evaluated a Chinese traditional medicine, alpinate oxyphyllae fructus (AOF), for therapeutic efficacy for treating Ang II-induced cardiac hypertrophy. AOF has been used to treat patients with various symptoms accompanying hypertension and cerebrovascular disorders in Korea. We investigated its protective effect against Ang II-induced cytoskeletal change and hypertrophy in H9c2 cells. The results showed that treating cells with Ang II resulted in pathological hypertrophy, such as increased expression of transcription factors NFAT-3/p-NFAT-3, hypertrophic response genes (atrial natriuretic peptide [ANP] and b-type natriuretic peptide [BNP]), and Gαq down-stream effectors (PLCβ3 and calcineurin). Pretreatment with AOF (60–100 μg/mL) led to significantly reduced hypertrophy. We also found that AOF pretreatment significantly suppressed the cardiac remodeling proteins, metalloproteinase (MMP9 and MMP2), and plasminogen activator, induced by Ang II challenge. In conclusion, we provide evidence that AOF protects against Ang II-induced pathological hypertrophy by specifically inhibiting the insulin-like growth factor (IGF) II/IIR-related signaling pathway in H9c2 cells. AOF might be a candidate for cardiac hypertrophy and ventricular remodeling prevention in chronic cardiovascular diseases.

THE IMPACT OF MORTALITY SALIENCE ON CONSUMERS’ RESPONSES TO LUXURY BRAND ADVERTISING EMBEDDED IN A CRISIS-INDUCING SOCIAL MEDIA CONTEXT

Shu-Chuan Chu,Tao Deng 글로벌지식마케팅경영학회 2021 Global Fashion Management Conference Vol.2021 No.11

Successful Enrichment of Rarely Found Candidatus Anammoxoglobus propionicus from Leachate Sludge

( Shu Chuan Hsu ),( Yen Chun Lai ),( Ping Heng Hsieh ),( Pun Jen Cheng ),( Suen Shin Wong ),( Chun Hsiung Hung ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7

Bacteria that mediate the anaerobic oxidation of ammonium (anammox) have been detected in natural ecosystems, as well as various wastewater treatment systems. In this study, sludge from a particular landfill leachate anaerobic treatment system was selected as the incubation seed for anammox microorganism enrichment owing to its possible anammox activity. Transmission electron microscopy observation, denaturing gradient gel electrophoresis analysis, and cloning/sequencing techniques were applied to identify the diversity of anammox microorganisms throughout the incubation. During the early stage of operation, the diversity of anammox microorganisms was similar to the original complex microbes in the seed sludge. However, as incubation time increased, the anammox microorganism diversity within the system that was originally dominated by Candidatus (Ca.) Brocadia sp. was replaced by Ca. Anammoxoglobus propionicus. The domination of Ca. Anammoxoglobus propionicus produced a stable removal of ammonia (70 mg-N/l) and nitrite (90 mg-N/l), and the total nitrogen removal efficiency was maintained at nearly 95%. The fluorescence in situ hybridization results showed that Ca. Anammoxoglobus propionicus was successfully enriched from 1.8 ± 0.6% initially to 65 ± 5% after 481 days of operation. Therefore, the present results demonstrated the feasibility of enriching Ca. Anammoxoglobus propionicus from leachate sludge, even though the original cell count was extremely low. Application of this seldom found anammox organism could offer an alternative to current ammonia-nitrogen treatment.

Scale Development and Model Validation for the Process of Exercise Engagement for People with Prediabetes

Chang, Shu-Chuan,Yeh, Hsiu-Chen,Kuo, Yu-Lun 한국간호과학회 2020 Journal of Korean Academy of Nursing Vol.50 No.2

Purpose: This study had two objectives: 1) to develop a scale for the process of exercise engagement (SPEE) for prediabetic individuals (PDIs); 2) to validate a structural model for the process of exercise engagement for PDIs. Methods: A cross-sectional survey with simple random sampling was conducted from September 2013 to December 2015 (in Taiwan). A total of 310 PDIs were enrolled for scale development and model validation via item analysis, factor analyses, and structural equation modeling. The Kuo model was used as the basis for developing the Chinese version of the SPEE for PDIs. Results: The SPEE contains five subscales with a total of twenty-one items that account for 54.9% to 65.9% of the total variance explained for assessing participants’ process of engagement during exercise. For Kuo model validation, the model measures indicated goodness of fit between the Kuo model and sample data. Analysis further revealed a direct effect between the creating health blueprints (CHB) stage and the spontaneous regular exercise (SRE) stage (b=.60). Conclusion: The SPEE includes five subscales for assessing the psychological transition and behavioral expression at each stage of the process of exercise engagement for PDIs. The SPEE for people with prediabetes provides deeper insights into the factors of behavioral change stages that are required to initiate long-term health care outcomes and avoid developing diabetes. These insights are significant as they allow for patient-specific mapping and behavior modification to effect exercise.

解构与超越: ― 台湾原住民女性作家主体性探索的新与变

李淑娟(Lee Shu chuan) 대한중국학회 2018 중국학 Vol.64 No.-

集體記憶・文化創傷 : 從《返校》系列談台灣的白色恐怖敍事

이숙연(LEE, Shu-Chuan)(李淑娟) 대한중국학회 2021 중국학 Vol.75 No.-

2017年初,台灣電遊《返校:detention》在全球遊戲平臺上綫,隨即熱銷廣受矚目。此遊戲以中學校園讀書會成員遭構陷入罪的情節為主幹,指涉台灣自50年代開始長達近40年的白色恐怖歷史。此歷史長期受壓抑,遲至90年代才逐漸浮現,但因政治因素導致真相隱晦難明。2016年,台灣‘轉型正義工程’正式展開,此現象才有所轉變。而在文學領域,受制于長期的記憶宰制,文本多呈現‘迷態敘事’特質,即内容斷裂、不明所以、表述閃爍其詞,這與台灣大衆對該歷史的關注程度一直呈現‘低溫稀薄’現象應有關聯。電遊《返校》之後,此現象有大幅轉變,隨後的同名電影和影集的上映更擴大了其影響面。筆者認爲,《返校》系列建構了台灣白色恐怖的集體記憶。本研究以集體記憶、文化創傷等概念,觀察白色恐怖歷史在此前不同形態的敘事中如何被建構、呈現,《返校》系列又以何種新的形態來呈現該記憶,及賦予其何種意義。 2017, Red Candle Games released a game called ‘Detention’ in the global gaming platform, ranking number three in sales in just one month. In the game lies a fictional story about members of a school book club who were framed in the 60 s under the Martial Law. It based on the historical event called the White Terror throughout the 50’s to the 80’s. White Terror had been a taboo in Taiwan for years, making the investigation of the event, damage, and accountability for a prolonged period of time. Since the termination of the Martial Law in 1987, the restoration of the forbidden history was commenced. Especially since 2016, Transitional Justice has become the core government policy, allowing the creation and execution of many policies in multiple aspects, such as fact-finding, restoration of records, rewriting history, compensation, and redemption of honor. Moreover, since the termination of the Martial Law, literature and personal biography based on the White Terror were created in the private sector. Nevertheless, it did not attract the attention of the general public. However, the release of ‘Detention’ has brought signs of change. ‘Detention’ grabbed the attention of teenagers who were distant with the history that happened 70 years ago. From the beginning of the release of the game, it has stirred up the internet and social media. In 2019, the game was made into a movie ‘Detention’. In addition, in December of 2020, ‘Detention’ made into a eight-series show. This is expected to create a collective memory of the White Terror in Taiwan. This study aims to find out how the White Terror in Taiwan is shown in various forms of narratives, using related concepts such as Assmann’s ‘Collective Memory’ and ‘Traumatic History’. Also, this study analyzes the new form of White Terror narrative of ‘Detention’ series.