제대혈 분리방법의 차이에 따른 단핵구 및 조혈모세포의 수득 효과에 관한 비교연구

박정숙,이영호,최안홍,노신애,김태겸,한진영 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1

연구배경 : 최근에 보고된 변형적 Ficoll-Hypaque 방법을 이용한 제대혈 분리방법이 지금까지의 고식적 Ficoll-Hypaque 분리방법에 비하여 조혈모세포의 수득효과면에서 얼마나 차이가 있는지 또한 비교적 수득효과가 크다고 알려져 있는 3% gelatin을 이용한 분리방법에 비하여 얼마나 효과적인지를 알아보고, 제대혈 분리방법의 차이에 따라 단핵구와 조혈모세포 사이에 어떤 상관관계가 있는지 알아보기 위하여 본 연구를 시행하였다. 방법: 21명의 건강한 산모로부터 정상 질식 및 제왕절개 분만 후 제대정맥에서 해파린 처리한 주사기를 사용하여 제대혈을 채취하였다 채취한 제대혈은 동일하게 3개월 시험관에 나누어 실온에서 24시간 이내에 고식적 Ficoll-Hypaque분리법, modified Ficoll-Hypaque 분리법, 3% gelatin 분리법을 이용하여 각각 단핵구 분리를 시도하였으며 이들의 단핵구수 및 생존률, CD34 양성세포수, 집락수를 측정 비교 하였다. 또 상관분석을 이용하여 단핵구수, CD34양성세포수와 CFU-GM집락수 사이의 상관관계를 알아보았다. 결과: 1) 세포 생존률은 고식적 Ficoll-Hypaque 분리법, 변형적 Ficoll-Hypaque 분리법, 3% gelatin 분리법 사이레 통계적 차이가 없었다. 2) 단핵구수는 3% gelatin으로 분리했을 때가 4.56±1.86 (×10^(6)/mL)로 수득효과가 가장 높았으며 (P=0.0001), Ficoll-Hypaque으로 분리했을때는 각각 1.54±0.06(×10^(6)/mL), 1.36±0.67(×10^(6)/mL)로서 고식적 방법과 변형적 방법 차이에 통계적 차이가 없었다. 3) CD34 양성세포수는 3%gelatin으로 분리했을 때가 6.08±3.99(×10⁴/mL)로 수득효과가 가장 높았으며 (P=0.0001), Ficoll-Hypaque으로 분리했을 때는 각각 2.18±2.17(×10⁴/mL), 2.24±1.63(×10⁴mL)로서 고식적 방법과 변형적 방법사이에 통계적 차이가 없었다. 4) CFU-GM 집락수는 3% gelatin으로 분리했을 때가 15.72±8.93(×10³/mL)로 수득효과가 가장 높았으며 (P=0.0002), Ficoll-Hypaque으로 분리했을 때는 각각 3.12±1.45(×10³mL), 2.35±0.82(×10³/mL)로서 고식적 방법과 변형적 방법 사이에 통계적 차이가 없었다, 5) 제대혈 고식적 Ficoll-Hypaque 방법으로 분리하였던 경우는 단핵구수와 CFU-GM집락수 사이에 상관관계가 없었으며CD34 양성세포수만 CFU-GM집락수와 상관관계가 있었다. (r=0.57). 또 변형적 Ficoll-Hypaque 방법에 의하여 분리하였던 경우는 단핵구수나 CD34 양성세포수 모두가 CFU-GM 집락수와 상관관계가 없었다. 그러나 제대혈을 3% gelatin법으로 분리하였던 경우는 CFU-GM집락수 가 단핵구수 (r=0.88) 및 CD34양성세포수(r=0.86)양자 모두와 상관관계가 있었다. 결론 : 3%gelatin을 이용하여 제대혈을 분리하는 것이 단핵구 및 조세모세포의 수득효과면에서 가장 효과적이었다. 한편 변형적 Ficoll-Hypaque 분리법이 고식적 Ficoll-Hypaque 분리법보다 시간과 경비면에서 비효율적이면서 수득효과도 높지 못하였다. 또 제대혈 분리법의 차이에 따라 단핵구나 CD34 양성세포와 VFU-GM사이에 상관관계에 차이가 있었으며, 실제로 제대혈이식 후에 생착 여부를 예측할 수 있는 인자가 제대혈 분리법의 차이에 따라 달라지는지에 관해서 보다 많은 임상적 연구가 필요할 것으로 사료된다. Background : Cord blood banking for stem cell transplantation requires volume reduction and red cell depletion for cost effective storage. To determine which technique could minimize the loss of progenitor cells during cord blood processing, we compared the efficacy of cord blood separating methods which were conventional Ficoll-Hypaque method, modified Ficoll-Hypaque method, and 3% gelatin method. Methods : Twenty-one cord blood samples were obtained with heparinized syringes from the umbilical vein following delivery. Three aliquots of each 20mL cord blood were stored at room temperature and processed by 3 different techniques within 24 hours after collection. We compared mononuclear cells(MNCs) counts and their viability, CD34+ cells counts, and CFU-GMs counts and also observed their correlations among three techniques. Results : 1) The yield of MNCs or CD34+ cells was highest in the cord blood processed with 3% gelatin method than conventional or modified Ficoll-Hypaque method(P=0.0001). 2) There were no significant difference in the yield of MNCs and CD34+ cells between conventional and modified Ficoll-Hypaque method. 3) Both MNCs counts (r=0.800) and CD34+ cells counts (r=0.86) were significantly correlated with CFU-GM counts in the cord blood processed with 3% gelatin method. 4) CD34+ cells counts were significantly correlated with CFU-GM counts in the cord blood processed with conventional Ficoll-Hypaque method (r=0.57), but MNC counts were not. 5) Both MNC counts and CD34+ cell counts were not correlated with CFU-GM counts in the cord blood processed with modified Ficoll-Hypaque method. Conclusion : Cord blood separation with 3% gelatin method could provide better yield of progenitor cells than with FICOLL_Hypaque methods. And between two Ficoll-Hypaque methods, modified method is more laborsome but not better in terms of clonogenic potential than conventional method.

알쯔하이머병 환자에서 백질 변화와 혈중 호모시스테인 및 엽산 농도의 상관관계

김신겸(Shin Gyeom Kim),정한용(Han Yong Jung),이소영(So Young Lee),신은영(Eun Young Shin),정우열(Woo Yeol Jung),박준호(Jun Ho Park) 대한노인정신의학회 2009 노인정신의학 Vol.13 No.2

Objectives : To explore the relationship of white matter changes (WMC) on magnetic resornance imaging (MRI) with plasma homocysteine and folate levels in Alzheimer's disease (AD) patients. Methods : Forty-one patients with probable AD were evaluated on WMC on brain MRI, plasma homocysteine and folate levels, and cerebrovascular risk factors. Subjects were divided into two groups according to WMC scores (lower group with <5 and higher group with ≥5). WMC were assessed by a scale of the European Task Force on Age-Related WMC. Results : Plasma homocysteine levels were correlated with right frontal WMC and have a tendency of correlation with left frontal WMC. When subjects were divided into two groups: higher plasma homocysteine group (≥14 μmol/L) and lower plasma homocysteine group (<14 μmol/L), the former was significantly higher than the latter in both frontal WMC. In a logistic regression analysis, higher plasma homocysteine were not a significant risk factor for higher WMC. Increasing age was a only significant risk factor for higher WMC and correlated with both frontal WMC. There was no relationship folate with any WMC. Conclusion : It is possible that plasma homocysteine levels have a region-specific correlation with frontal WMC in AD. However, it is important that effect of age on the relationship should be considered.

치매 개념의 역사적 변천

김신겸(Shin Gyeom Kim),정한용(Han Yong Jung) 대한노인정신의학회 2008 노인정신의학 Vol.12 No.1

Most histories of senile dementia begin with Alois Alzheimer's description in 1906 of the first case of Alzheimer's disease. However, the history of senile dementia is quite rich, dating back to the ancient Greek and Roman philosophers and physicians, so the history of dementia is probably as old as mankind itself. Although senile dementia has been recognized as a condition of aged individuals since at least the time of Pythagoras in the 7th century B.C., because it was dismissed as an inevitable feature of aging, it remained largely an uninvestigated disorder until the 19th century. An introduction of a scientific approach to clinical observation and the systematized classification of mental disorders in the mid-19th century that senile dementia began to be differentiated from other dementias, and was established as its own defined class of mental disorder. The identification of Alzheimer's disease at the onset of the 20th century was a turning point for the understanding of senile dementia, and the concepts and histological findings presented by the early researchers of Alzheimer's disease remain relevant still today. Here the author traces the history of the evolution of our current conceptualization of Alzheimer's disease from the Greek-Roman concept of age-associated dementia.

노인에서의 약물 유도성 섬망

김신겸(Shin Gyeom Kim),정한용(Han Yong Jung) 대한노인정신의학회 2008 노인정신의학 Vol.12 No.2

The elderly is accompanied by an increased likelihood of illness, and old people take a disproportionate amount of self-administered and prescribed medications. They are more likely to develop delirium as a result of taking medications than younger patients. Psychoactive drugs such as narcotics, long acting benzodiazepines, and tricyclic antidepressants are important causes of delirium. Drug induced confusion with nonpsychoactive drugs is often idiosyncratic in nature, and the diagnosis is easily missed. Anticholinergic medications are the most common causes of drug induced delirium in the elderly. Successful treatment of delirium depends on identifying the contributing factors, and drugs are the most common reversible cause of delirium. This article provides an clinical approach to prevent, recognize, and manage drug induced delirium.

루이체 치매

김신겸(Shin Gyeom Kim),정한용(Han Yong Jung) 대한노인정신의학회 2007 노인정신의학 Vol.11 No.2

Here the author reviews the clinical and pathologic characteristics of dementia with Lewy bodies (DLB). DLB took many years to crystallize into a recognizable clinico-pathologic entity. Based on sensitive immunostaining technique, DLB is now considered the second most commonest cause of neurodegenerative dementia in the elderly. It is part of the range of clinical presentations that share a neuritic pathology based on abnormal aggregation of the synaptic protein α-synuclein. Lewy body pathology is found from the brainstem to the cortex and, in many cases, associated with concurrent Alzheimer' disease pathology. A recent international consortium on DLB has resulted in revised criteria for the clinical and pathological diagnosis of DLB incorporating new information about the clinical features and improved methods for their assessment. Neuropathologic diagnosis now assigns a weight to both α-synuclein and Alzheimer tangle pathology. Accurate identification of patients is important because they have specific symptoms, impairments, and functional disabilities that differ from those of other dementing illness including Alzheimer's disease.

Cellulase전처리가 펄프 고해에 미치는 영향

신원겸(Won-Gyeom Shin),최윤혁(Yoon-Hyuck Choi),신수정(Soo-Jeong Shin) 한국펄프·종이공학회 2023 한국펄프·종이공학회 학술발표논문집 Vol.2023 No.10

Common Trigger Points on College-Level Athletes with Various Sports

Joo Hyun Park,Hee Joon Shin,Sa Gyeom Lee,Geon Cheol Lee,Hee Jong Yoon,Wan Sung Hong,Bong Oh Goo,Dong Yeop Lee,Hyung Soo Shin,Bum Chul Yoon 국제물리치료학회 2010 Journal of International Academy of Physical Ther Vol.1 No.1

The purpose of this study was to investigate the distribution of trig˗ ger points(TrPs) on athletes with various sporting background. To achieve the purpose, a study was carried out through a survey from 180 athletes involved in 6 selected sports at Yong-In University. Selected sports included Judo, Taekwondo, Kendo, Ssi-reum(Korean traditional wrestling), Boxing, and Golf. An interview type survey and physical examination were conducted with each thirty athletes from each of the selected sports groups. Technical statistic(SPSS 15.0) was used to analyze the distribution of TrPs on these athletes. The most common TrPs observed in muscles of Trapezius, Quadratus Lumborum, Quadriceps in Judo. In Taekwondo, it was on the trapez˗ ius and triceps surae. Kendo athletes had TrPs at sites of trapezius, brachioradialis and triceps surae. Ssirem athletes were found to have TrPs on trapezius, deltoid and quadrates lumborum. In boxers, TrPs appearing at trapezius and brachioradialis were observed. Finally, Golf players were seen to have TrPs at trapezius, quadrates lumbo˗ rum and brachioradialis. Hence, the analysis shows that there are significant differences of the distribution of TrPs according to the different sport items of the athlete.

Behavioral analysis of the olive flounder (Paralichthys olivaceus) against the periphyton attachment to underwater structure of the wind power plants

Gyeom Heo,Hyeon-Ok Shin 한국수산해양기술학회(구 한국어업기술학회) 2021 한국수산해양기술학회 학술발표대회 Vol.2021 No.11

저급원료와 산업폐기물을 활용한 세라믹스 담체 개발

신대용,윤봉구,김승겸 강원대학교 부설 석재복합신소재 제품연구센터 2003 석재연 논문집 Vol.8 No.-

석탄회와 벤토나이트 및 이스트 분말의 성형체를 800-1,000℃에서 1시간 소성하여 수질정화용 미생물 고정화 세라믹 담체를 제조하여 기공ㆍ기계적 특성을 조사하였다. 석탄회와 벤토나이트(FB)시편은 벤토나이트의 첨가량과 소성온도가 증가함에 따라 기공특성은 감소하였으나 압축강도는 증가하였다. 800-1,000에서 소성한 FB시편은 압축강도 89.6~128.9kgf/㎠, 부피비중 1.25-1.43, 겉보기비중 1.61~l.78, 기공률 27.2~62.2%, 평균기공경 7.9-25.6㎛, 기공용적 8.9~22.2×10^(-5)㎤/g 및 비표면적 35.2~134.3㎡/g을 나타내었다. 이스트 분말을 첨가한 FBY시편은 FB시편에 비하여 기공특성이 향상되었으나 압축강도는 감소하였다. 9F1B시편에 10 wt%의 이스트 분말을 첨가하여 900℃에서 1시간 소성한 9F1B1Y시편은 압축강도 98.7 kgf/㎠, 부피비중 1.20, 겉보기비중 1.67, 기공률 68.1%, 평균기공경 48.9㎛, 기공용적 29.5×10-5㎤/g 및 152.2㎡/g의 비표면적을 나타내었으며, 담체의 기공에 S. saprophyticus의 부착특성이 양호하여 수질정화용 미생물 고정화 담체로 이용이 가능하였다. Porous ceramic supports with immobilized microorganisms for the water purifier were synthesized by firing green compacts of mixed powder comprising of fly ash, bentonite and an additive of yeast powder at 800-1,000℃ for Ih, and the pore and mechanical properties of specimens wen investigat ed. The compressive strength was increased in FB(fly ash plus bentonite) specimens while pore properties was decreased with increasing the bentonite content and sintering temperature. The compressive strength, bulk density, apparent density, porosity, mean pore size, pore volume, and specific surface area of FB specimens at 800~l,OOO℃ were 89.6~128.9kgf/㎠, 1.25~1.43, 1.61~l.78, 27.2~62.2%, 7.9~25.6㎛, 8.9~22.2×10^(-5)㎤/g and 35.2~134.3㎡/g, respectively. The pore properties of FBY(FB+yeast powder) specimens were superior to that of FB specimens, however compressive strength was decreased with increasing yeast powder content. The overall properties of 9F1B1Y(9F1B +10% of yeast powder) specimens at 900℃ for 1 h were 98.7kgf/㎠, 1.20, 1.67, 68.1%, 48.9㎛, 29.5×10-5㎤/g and 152.2㎡/g, respectively. In this study, it was revealed that 9F1B1Y specimen demonstrated better S. saprophyticus adherence properties on their surface pores. Consequently, the microorganisms immobilized on porous ceramic supports showed better water purifying performance with many pores and adequate strength.

Behavior analysis of rockfish (Sebastes inermis) corresponding to the wavelength of LED light

Gyeom Heo,Hyeon-Ok Shin,Min-A Heo 한국수산과학회 양식분과 2014 한국수산과학회 양식분과 학술대회 Vol.2014 No.7