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      • 효소 중합반응에 의한 인공 cDNA 클론의 형성

        유시현,권무식 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

        The sequence-specific DNA binding protein, PU.1 common to macrophage and B cell in mammals, is one of the transcription activators. Its DNA binding activity seems to correlate with the phosphorylation of specific serine residue(s). It is, however, not clear that which serine(s) could be phosphorylated for the activation of PU.1. To understand the phosphorylation mechanism of the transcription factor, the first nucleotide coding the 11th amino acid [TCC] of PU.1, "Thymidine", was substituted with "Guanidine" by site-directded mutagenesis. The mutant PU.1 cDNA restricted by Xba I and Hind III, was ligated into Xbal-HindIII cut pBluescript KS+, and then transformed into E.coli JM109 to generate a new mutant clone of PU.1. The mutant will be instrumental in evaluating a plausible phosphorylation site of PU.1.

      • KCI등재

        남한 내 북한이탈주민의 3년간 사회적응 추적 연구 : 2001년부터 2004년까지 생활과 교육을 중심으로 With prioty given to life of North Korea defectors from 2001 to 2004

        유시은,전우택,조영아,홍창형,엄진섭 연세대학교 통일연구원 2005 통일연구 Vol.9 No.1

        Purpose of this study : In 2001, this study that investigate actual conditions about adaptation degree of Korean social life of the North Korean defectors was carried out to 151 people that succeed in chases among 200 the North Korea defectors answer in study. Purpose of this study is as following. First, we wished to search change aspect of actual conditions about South Korea society adaptation of the North Korean defectors who take part in the first investigation for past 3 years. Second, we wished to search difference of adaptation aspect by populational and social element. Third, We wished to suggest things that can be beaconed to efficient support policy of government or private organization and successful South Korea social adaptation method which support the North Korean defectors on the basis of study finding about change of adaptation aspect of the North Korea defectors. Methods of Study : We designated 200 Panels to study and survey actual adaptation conditions of the North Korean defectors on South Korea society synthetically and for long-term. So, chasing about 151 people was possible in 2004. We are fertilized and supplement the questionnaire forming in 2001 and composed the new questionnaires to part of a life, mental state, Body and health. This study among them investigated a standard of living of the North Korean defectors. In this process, interviewer directly asked and recorded questions to reduce individual variation of people who receive interview. Study Results : Government's residence support policy about North Korean secession inhabitants was generally attained as effective and suitable for North Korean inhabitants' early South Korea settlement. Only, government need to discuss about North Korea secession inhabitants' residence support more variously for long-term. Second, education should be supported more actively and variously. Suitable point of education for their needs should make a effect of vocational training or education maximize. This is a very important part. Third, they need guidance and education that can receive more easily law support. Fourth, medical supports and health cares should be achieved continuously and systematically to them since they arrived in South Korea. To supports and studies North Korean secession inhabitants has the large significance to gain a foothold about reunification of North and south. Their South Korea society adaptation is becoming topic of conversation among South Korean. An atmosphere of unity is elated. And interest degrees about North Korean are actually rising as exchanges between South and North Korean is activated. Therefore, the adaptation study about North Korean secession inhabitants will have to be expanded in studies for South Korean, not to be limited in studies for North Korean.

      • KCI등재
      • 주석中毒의 解毒劑에 관한 硏究

        沈英燮,柳時馨 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.2

        This study is to examine the detoxication effect of cholic acid, methionine, ginseng ex. and glycyrrhizin on the toxicity induced by tin. For this purpose, this paper provides an analysis of the data on the groups of rats, one group of rats treated with tin (30 mg/kg body weight) only and other groups of rats combined treated with cholic acid, methionine, ginseng ex. and glycyrrhizin added tin. After breeding for 3 days, the data on pH, total acidity and volume of gastric juice secreation, changes in glucose and cholesterol levels, activity of GOT, GPT and alkaline phosphatase and concentration of calcium in kidney are analysed. The results were summarized as follow: 1. The volume, total acidity and pH of gastric juice showed a remarkable reduction in rats administered orally with tin. However, the administration of ginseng ex. or glycyrrhizin to the tin treated rats significantly enhanced the elevation of volume, total acidity and pH of gastric juice. 2. The serum glucose and cholesterol levels in rats were slightly lowered by oral administration of tin. Also, the administration of detoxicant to the tin treated rats were not significantly altered the elevation of serum glucose and cholesterol levels in rats. 3. The activity of serum GOT and GPT were markedly increased by administration of tin in rats. However the administration of detoxicant to the tin treated rats significantly inhibited the elevation of serum GOT and GPT. 4. The calcium concentration of kidney were segnificantly increased by administration of tin in rats. However, the administration of detoxicant markedly reduced the increased kidney calcium.

      • KCI등재

        북한이탈주민의 사회적응 7년 추적 연구 : 생활과 교육을 중심으로

        전우택,유시은,엄진섭,김희진 연세대학교 통일연구원 2009 통일연구 Vol.13 No.1

        The purpose of this study is to analysis the change patterns of life style and education of North Korean defectors. The questionnaire of this study is for two hundred North Korean defectors who had entered South Korea in 2001. The questions are about residence, education, legal issue, and health. According to this study, the residences of the defectors were still concentrated on the Metropolitan area. Type of residence has become varied so as to increase proportion of their own-houses and long-term leases. Males had got educated for employment purpose, while females had learned using computer the most. Among age groups, the 20s and the 30s had taken regular education the most. As the length of staying in South Korea had increased, the legal cases had decreased; however, the legal issues had become more complicated. The number of the defectors with chronic diseases has gradually declined; thus, overall health status of the defectors had become better. As the length of staying in South Korea had increased, their needs for supports of basic life had decreased, while the demands for consultation about marriage and legal issues. In the conclusion, we point out several ideas based on this study result, which can be utilized in developing policies and supporting programs for North Korean defector adaptation in South Korea.

      • KCI등재

        남한 내 북한이탈주민들의 3년간 사회의식 변화 및 생활 만족도에 대한 추적연구

        전우택,유시은,조영아,홍창형,엄진섭,서승원 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.3

        Objectives : The aim of this study was to examine the change in attitudes and satisfaction level of North Korean defectors in South Korea over a 3 year period (2001-2004). Methods : In our first study in 2001, a total of 200 detectors were interviewed. In the follow up study after 3 years, 151 defectors were re-interviewed by an in-person interview. Results : The level of satisfaction to the governmental support policy to defectors decreased significantly. However, the general level of satisfaction increased slightly (from 3.52 to 3.56 ; 5-score scale). This shows that North Korean defectors are stably adapting to South Korean society. The level of satisfaction significantly correlated with the defectors' confusion of the different value systems, the defectors' understanding of the South Korean people, satisfaction with their physical and mental health, and the Prejudice of South Korean people to defectors. In terms of the attitudes, the type of schooling, the military service and the communist party membership in North Korea were not related to the change in attitude. However, there was a significant correlation with gender and age. Women showed a greater change in attitude compared to men. Older people had difficulty in understanding the South Korean people, but they showed little psychological affiliation to North Korea. Moreover, they showed a high level of satisfaction with the economic situation in South Korea and had an optimistic viewpoint for their future than younger people. Even though many defectors attended churches, religion was not associated with the level of satisfaction or their change in attitude. Conclusion : The factors that influence the level of satisfaction and attitudes of North Korean defectors have changed over 3 years. Therefore, the government, nongovernmental organizations (NGOs) associated with defectors and the churches need to be more sensitive to these changes and make plans to support the adaptation of defectors to South Korean society.

      • KCI등재

        An exploration of the impact of macro-level governing structure on local public sector employment in the United States

        Yu Shi 한국행정학회 2018 International Review of Public Administration Vol.23 No.1

        This research investigates how characteristics of the macro-level governing structure affect local public sector employment. The empirical model is tested using fixed effects with panel data that consist of 3031 county-type areas in the United States for five year intervals from 1992 to 2012. We find that an increased level of fiscal decentralization is associated with larger labor input in the production of public services. Our statistical results also show that interjurisdictional competition leads to the growth of local public sector employment. Nevertheless, we do not find that spatial fragmentation and jurisdictional overlap have any significant relationship with the level of public sector employment.

      • KCI등재

        A Study on the Management Efficiency of “Seolhyang” strawberries Farms Used SBM-DEA Model with Undesirable Output

        Yu-Cong Sun(손우총),Shi-Yong Piao(박세영),Shuang-Yu Hu(호쌍우),Sung-Chan Kim(김성찬),Jong-In Lee(이종인) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.7

        본 연구에서는 농촌진흥청에서 조사한 ‘표준소득자료집’의 99명의 설향 딸기농가 조사사료를 이용하여 분석하였다. 이러한 자료로 SBM-DEA 모델을 활용하여 이산화탄소 배출량을 고려한 설향 딸기농가의 경영효율성 분석을 실시하였다. 분석결과 99명 농가 중 14명(14.14%) 농가의 기술효율성은 불변규모수익(CRS)일 경우, 효율적인 것으로 나타났다. 반면, 가변규모수익(VRS)의 경우, 99명 농가 중 30명(30.3%) 농가의 순수기술효율성은 효율적인 것으로 나타났다. TEVRS 가설, TECRS 가설, SE 가설을 통해 분석한 결과, 설향 딸기농가의 평균기술효율성은 0.53, 0.35, 0.60으로 나타났다. 그리고 Tobit모형을 통해 순수기술효율성에 영향을 미치는 요인을 분석하였다. 분석 결과 이산화탄소 배출, 난방여부, 재배면적 등 요인이 순수기술효율성에 부정적인 영향을 미치는 것으로 나타났다. 반면, 유기 비료의 비율은 높을수록 순수기술효율성이 높아지는 것으로 나타났다. 이는 유기비료를 많이 사용할수록 순수기술효율성에 긍정적인 영향을 미치는 것을 의미한다. 또한, 딸기농가의 재배경력은 순수기술효율성에 통계적으로 영향을 미치지 않는 것으로 나타났다. 따라서, 살충제 및 농약비용을 줄여야 하며, 지속가능한 딸기 생산을 위해 유기비료를 사용해야한다. 또한, 이산화탄소 배출 감소에 관한 농업정책을 설립할 필요가 있다. Ninety-nine strawberry farmers were assessed. A slacks-based and data envelopment analysis measure (SBM-DEA) of efficiency with an undesirable output was used to analyze the technical efficiency of farmers by taking carbon dioxide (CO2) as the undesirable output. Only 14 (14.14%) were technically efficient under a constant return to scale (CRS) and scale efficiency, while 30 (30.3%) households were technically efficient under variable return to scale (VRS). On average, the estimated technical efficiency scores of "Seolhyang" strawberry farmers in the study area under the technical efficiency of the CRS (TECRS) assumption, technical efficiency of the VRS (TEVRS) assumption, and scale efficiency (SE) assumption were approximately 0.35, 0.53, and 0.60, respectively. The Tobit regression model was used to identify the factors influencing the technical efficiency of "Seolhyang" strawberries. CO2 emissions, heating or not, and the land area negatively affected the technical efficiency, while the organic fertilizer ratio positively affected the technical efficiency. The effects of strawberry planting experience on the technical efficiency were not statistically significant. Therefore, agricultural policies should include measures to reducing the use of pesticides and fuels that emit high emissions and improve the use of organic fertilizers for sustainable strawberry development.

      • KCI등재

        Ankrd7, a Novel Gene Specifically Expressed in Sertoli Cells and Its Potential Roles in Sertoli Cell Maturation

        Yu-Qiang Shi,Lian-Cai Du,Qing-Zhong Wang,Chun-Fang Han 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.2

        The somatic Sertoli cells play an essential role in testis determination and spermatogenesis by providing nutrition and structural support. In the current study, we report on the novel Ankrd7 gene that contains five ankyrin repeat domains. This gene was specifically expressed in Sertoli cells and was regulated in a maturation-dependent manner. Its expression was restricted to testicular tissue, and its mRNA could be detected in testes at as early as 14 dpp (days post partum) using RT-PCR analysis. In both testicular tissue sections and in vitro cultured Sertoli cells, the Ankrd7 protein was localized to the nucleus of the Sertoli cell. Immunohistochemistry and immunocytochemistry investigations showed that the protein was detectable in testicular tissues at 20 dpp, at which time Sertoli cells were gradually differentiating into their mature cellular form. These results suggest that Ankrd7 is probably involved in the process of Sertoli cell maturation and in spermatogenesis.

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