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서세영,김미혜 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.1
전자상거래 등 보안을 요구하는 정보보호 시스템 분야에서는 공개키 기반구조(PKI: Public Key Infrastructure)가 폭넓게 사용되고 있다. PKI 도메인들이 더 많이 설립될수록 더 많은 상호 인증을 필요로 하게 된다. 더구나 각 국은 더욱 복잡한 상호 인증을 필요로 하는 많은 인증기관을 가지고 있다. 그러므로 상호 인증을 위한 경로 탐색시 최단시간에 인증 경로를 찾을 수 있는 알고리즘이 필요하다. 본 논문에서는 위임티켓을 이용한 위탁 인증 방식으로 에이전트들과 이동 노드간의 직접적인 인증을 가능하게 하고, 핸드오프시 오버헤드를 최소화 하여 인증시간을 단축하였다. In the field of secure information systems including electronic commercials, public key infrastructure(PKI) is widely used for secure services. The more PKI domains are established, the more needs are required for cross-domain certifications. Furthermore, each country has many certificate authorities(CA) which requires more complex cross certification. We may need a fast algorithm in order to find the possible certification paths. In this paper, we reduced certification time by reducing overhead of handoff and the direct certification between agents and mobile nodes
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박영실 동아대학교 동아시아연구원 2011 동아시아 : 비교와 전망 Vol.10 No.2
중국에서 농민공(農民工)은 개혁개방과 함께 등장하여 현재는 거대한 계층으로 성장한 노동집단이다. 농민공은 사회주의 신 중국 농촌의 건설자이며 국민경제발전에 거대한 공헌을 하였다. 최근에는, 이들 농민공 중에서도 젊은 신세대 농민공 계층이 중국 정부의 큰 관심을 받고 있다. 신세대 농민공은 제1세대 농민공에 비한다면 도시 주민에 더 가깝다. 그들의 생활방식 및 가치관은 제1세대 농민공과 비교하여 상당한 차이를 보이는데, 도시 생활을 동경하며 도시에서 더 많은 발전 기회를 얻고자 한다. 그러나 도농이원화체제로 인해 신세대 농민공들은 농민의 신분으로서 도시 주민들과 같은 사회보장권익을 향유할 수 없다. 신세대 농민공은 중국 사회 발전에 있어 중요한 원동력이다. 따라서 그들의 사회적 권익은 더욱더 보호 받아야 한다. 본문은 사회 현실에 입각하여, 신세대 농민공의 특징 및 시민화 과정에서의 사회 보장 결핍으로 인한 권익 침해 원인을 분석 연구하고 사회 보장의 필요성을 살펴보고 실행 가능한 해결책을 제시하여 보고자 한다. In China, peasant migrants are labor groups which grew as a huge class with the appearance of reform and market opening. These peasant migrants have built a new socialist Chinese farming area, and also an important driving force of constructing urban areas, greatly contributing to Chinese national economic development. In recent years, the Chinese government is putting great focus on new generation migrant wokers among the total peasant migrants. Compared to the 1st generation peasant migrants, these new generation migrant wokers are close to urban residents. Their life styles and values are greatly different from those of the 1st generation's, aspire urban life, and want to seize more developing opportunities in the city. However, due to town and village separation system, new generation migrant wokers are in fact farmers, and cannot enjoy the same social security rights as the urban citizens do. The new generation migrant wokers should enjoy more social security rights as the main driver of China's social development. In this regard, this thesis considers the current status and its reasons why new generation peasant migrants lag far behind in terms of social security rights during the citizenship process. In addition, this thesis suggests feasible solutions by reviewing the necessity of the social security, As the main force of city consruction, new generation migrant workers have also played Connecting roles in their family. New Generation Migrant Wokers bring more and more social problems with their coming. Therefore, how to protect Generation Migrant Wokers social security rights is worth to be discussed and solved. Only in this way, the social stability will be kept.
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단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석
양영실,차준영,네티 엘마와티,정민희,배동원,이창원,손대영 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching. We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.
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퉁퉁마디로부터 색소체 외막 단백질 유전자의 분리 및 발현분석
네티 엘마와티,차준영,양영실,정민희,신동진,이병현,이곤호,손대영 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
Complementary DNA encoding chloroplast outer envelope membrane protein (OEP) from the halophyte Salicornia herbacea has been cloned and sequenced. The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa. The expression level of ShOEP increased by salt, drought and ABA treatments. ShOEP expression was largely induced in roots and shoots by high salts. The biological function of ShOEP was examined by yeast complementation. ShOEP can suppress Na+ sensitivity of yeast mutant (cnbΔ) in the presence of salt. These results suggest that ShOEP is a salt inducible gene and may have functions in the regulation of plant salt stress.
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Ying-wu Shi,Kai Lou,Chun Li,Lei Wang,Zhen-yong Zhao,Shuai Zhao,Chang-yan Tian 한국미생물학회 2015 The journal of microbiology Vol.53 No.10
We used Illumina-based 16S rRNA V3 amplicon pyrosequencing to investigate the community structure of soil bacteria from the rhizosphere surrounding Salicornia europaea, and endophytic bacteria living in Salicornia europaea plants and Sueada aralocaspica seeds growing at the Fukang Desert Ecosystem Observation and Experimental Station (FDEOES) in Xinjiang Province, China, using an Illumina genome analyzer. A total of 89.23 M effective sequences of the 16S rRNA gene V3 region were obtained from the two halophyte species. These sequences revealed a number of operational taxonomic units (OTUs) in the halophytes. There were between 22–2,206 OTUs in the halophyte plant sample, at the 3% cutoff level, and a sequencing depth of 30,000 sequences. We identified 25 different phyla, 39 classes and 141 genera from the resulting 134,435 sequences. The most dominant phylum in all the samples was Proteobacteria (41.61%–99.26%; average, 43.30%). The other large phyla were Firmicutes (0%– 7.19%; average, 1.15%), Bacteroidetes (0%–1.64%; average, 0.44%) and Actinobacteria (0%–0.46%; average, 0.24%). This result suggested that the diversity of bacteria is abundant in the rhizosphere soil, while the diversity of bacteria was poor within Salicornia europaea plant samples. To the extent of our knowledge, this study is the first to characterize and compare the endophytic bacteria found within different halophytic plant species roots using PCR-based Illumina pyrosequencing method.
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Ying Shi Liang,Hee-Jin Bae,Sang-Ho Kang,Theresa Lee,Min Gab Kim,Young-Mi Kim,하선화 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.4
To efficiently express a gene of interest in transgenic plants, the choice of promoter is a crucial factor as it directly affects the expression of the transgene that will yield the desired phenotype. The Arabidopsis b-carotene hydroxylase 1 gene (AtBch1) shows constitutive and ubiquitous expression and was thus selected as one of best candidates for constitutive promoter analysis by both in silico northern blotting and semi-quantitative RT-PCR analysis. To investigate AtBch1 promoter activity, the 1,981-bp 50-upstream region of this gene was fused with b-glucuronidase (GUS) and transformed into Arabidopsis. Through the molecular characterization of transgenic leaf tissues, the AtBch1 promoter generated strong activity that drives 1.8- and 2-fold higher GUS expression than the cauliflower mosaic virus 35S (35S) promoter at the transcriptional and translational levels, respectively. Furthermore, the GUS enzyme activity driven by the AtBch1 promoter was 2.8-fold higher than that produced by the 35S promoter. By histochemical GUS staining, the ubiquitous expression of the AtBch1 promoter was observed in all tissues of Arabidopsis. Semi-quantitative RT-PCR analysis with different tissues further showed that this promoter serves as a strong constitutive driver of transgene expression in dicot plants. To efficiently express a gene of interest in transgenic plants, the choice of promoter is a crucial factor as it directly affects the expression of the transgene that will yield the desired phenotype. The Arabidopsis b-carotene hydroxylase 1 gene (AtBch1) shows constitutive and ubiquitous expression and was thus selected as one of best candidates for constitutive promoter analysis by both in silico northern blotting and semi-quantitative RT-PCR analysis. To investigate AtBch1 promoter activity, the 1,981-bp 50-upstream region of this gene was fused with b-glucuronidase (GUS) and transformed into Arabidopsis. Through the molecular characterization of transgenic leaf tissues, the AtBch1 promoter generated strong activity that drives 1.8- and 2-fold higher GUS expression than the cauliflower mosaic virus 35S (35S) promoter at the transcriptional and translational levels, respectively. Furthermore, the GUS enzyme activity driven by the AtBch1 promoter was 2.8-fold higher than that produced by the 35S promoter. By histochemical GUS staining, the ubiquitous expression of the AtBch1 promoter was observed in all tissues of Arabidopsis. Semi-quantitative RT-PCR analysis with different tissues further showed that this promoter serves as a strong constitutive driver of transgene expression in dicot plants.
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Further study on improvement on strain concentration in through-diaphragm connection
Ying Qin,Jing-Chen Zhang,Peng Shi,Yi-Fu Chen,Yao-Han Xu,Zuo-Zheng Shi 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.39 No.2
Hollow structural section (HSS) columns have been increasingly popular due to their structural and architectural merits. However, practical difficulty lies in developing proper connections. The through-diaphragm connections are considered as suitable connection type that is widely adopted in Asian countries. However, the stress concentration occurs at the location connecting through-diaphragm and steel beam. Furthermore, the actual load path from the beam flange is not uniformly transferred to the HSS column as conventionally assumed. In this paper, tensile tests were further conducted on three additional specimens with beam flange plate to evaluate the load versus displacement response. The load-displacement curves, yield and ultimate capacity, ductility ratio were obtained. Furthermore, the strain development at different loading levels was discussed comprehensively. It is shown that the studied connection configuration significantly reduces the stress concentration. Meanwhile, simplified trilinear load-displacement analytical model for specimen under tensile load was presented. Good agreement was found between the theoretical and experimental results.
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Ying-ying Zhang,Ru-yu Xia,Shi-bing Liang,Xiao-yang Hu,Meng-yuan Dai,Yi-lin Li,Le-yi Zhao,Michael Moore,Yu-tong Fei,Jian-ping Liu 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.3
Background: Shufeng Jiedu capsule has been widely used in China for acute upper respiratory tract infections (AURTIs). The aim of this study was to evaluate its effectiveness and safety for AURTIs. Methods: Randomized controlled trials comparing SFJD with conventional drug for patients with AURTIs were included. Eight databases were searched from their inceptions to February 2021. Data was synthesized using risk ration (RR) or mean difference (MD) with their 95% confidence interval (CI). The primary outcome was resolution time of typical symptoms. Results: Twenty-five RCTs involving 3410 patients were included. SFJD in combination with conventional drug was associated with; in common cold shortening the duration of fever (MD −1.54 days, 95% CI [−2.15,−0.92], I2 = 80%, n = 385, 3 trials) and cough (MD −1.22 days, 95% CI [−1.52, −0.93]); in herpangina, shortening the duration of fever (MD -0.68 days, 95% CI [−1.15, −0.21], I2 = 68%, n = 140, 2 trials) and blistering (MD −0.99 days, 95% CI [−1.23, −0.76], n = 386, 3 trials); in acute tonsillitis and acute pharyngitis shortening the duration of fever (MD −1.13 days, 95% CI [−1.36, −0.90], I2 = 33%, n = 688, 7 trials) and sore throat (MD −1.13 days, 95% CI [−1.40, −0.86], I2 = 84.1%, n = 1194, 10 trials). SFJD also improving their cure rate with a range (1–5 days). No serious adverse events were reported. Conclusion: Low certainty evidence suggests that SFJD appears to shorten the duration of symptoms in AURTIs, improve cure rate and seems safe for application. However, high quality placebo controlled trials are warranted to confirm its benefit.
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