A study on the inflammatory response induced by LPS of the Arthrospira plateensis ethanol extract

Shi Jie Zhang,양재찬,김보애 한국응용과학기술학회 2019 한국응용과학기술학회지 Vol.36 No.3

Arthrospira platensis has been reported to contain a variety of substances such as phycocyanin, β-carotene, vitamin E and other carotenoids. In this study, zebrafish were treated with indoor cultivation spirulina ethanol extracts(ICAE) to determine toxicity(coagulation rate, hatching rate, heart rate). We used the DCFH-DA staining method to detect the effect of reactive oxygen species(ROS) generation on lipopolysaccharide(LPS)-induced zebrafish embryos ROS various concentrations(0.01, 0.05, 0.1, 0.5mg/ml) of ICAE. Cell toxicity was measured by WST-1 assay on RAW 264.7 macrophage cell line. Also, measured the inhibitory effect of nitric oxide(NO) and prostaglandin E₂(PGE₂) production in RAW264.7 macrophages induced by LPS at various concentrations of ICAE. The results of embryo coagulation rate, hatching rate, heart rate of zebrafish at various(0.01, 0.05, 0.1mg/ml) of ICAE was no toxicity. The ICAE treated group had an inhibitory effect on NO and PGE₂ production compared and decreased with concentration. The results of this study ethanol extract of Arthrospira platensis has an anti-inflammatory effect and suggest that is worthy of use cosmetics for skin protection.

Elucidating synthesis of noble metal nanoparticles/graphene oxide in freescavenger γ-irradiation

Jie Shi,Wanwei Jiang,Liangsen Liu,Miaolei Jing,Fengyan Li,Zhiwei Xu,Xing-xiang Zhang 한국물리학회 2019 Current Applied Physics Vol.19 No.7

Three representative noble metal (Ag, Au and Pt) nanoparticles decorated graphene oxide (NMNPs/GO) composites were fabricated via γ-irradiation without scavenger. The NMNPs/GO composites exhibited the pure and well-dispersed particles structure, which directly illustrated that the GO could be acted as scavenger to benefit reduction and growth of NMNPs. Compared with irradiated GO (IGO), the GO substrate of composites had the lower relative content of hydroxyl groups, meanwhile, the relative contents of carbonyl groups (Pt > Au > Ag) were increased with increasing valence of noble metal. Such results illustrated that the abundant hydroxyl groups could convert the hydrated electrons, hydroxyl and hydrogen radicals to promote the growth of NMNPs on the surface of GO. In addition, the Raman signals of Ag NPs/GO composites were significantly enhanced (6.18 fold more than pristine GO, respectively), exhibiting obvious surface-enhanced Raman scattering activity. Therefore, this paper revealed that the GO could convert the hydrated electrons and radicals to synthetizing NMNPs/GO composites during γ-ray irradiation.

中國産品責任賠償範圍硏究 -以侵權責任法與判例爲硏究視角-

( Shi Jie Xu ),( Kyu Yong Park ) 홍익대학교 법학연구소 2014 홍익법학 Vol.15 No.4

Chinese Tort Liability Law, which was enacted in 2010, has a great influence to the Chinese tort liability system, including the products liability. Before Tort Liability Law, the adjustments of product liability laws are General Principles of the Civil Act, Consumer Protection Law, Product Quality Law and so on. These laws have a positive effect on protecting the rights of victims, but still not enough. The execution of Tort Liability Law became an important role as a complementary product liability legal system. After the Tort Liability Law, Consumer Rights Protection Law was amended; Food and Drug Judicial interpretation of the Supreme People`s Court has also been announced to take effect in March 15, 2014. It is necessary to study on the impact of these laws and judicial interpretation on the Tort Liability Law and Product Liability legal system, the relationship between these regulations and Product Quality Law, other judicial interpretation, the scope of compensation for damages of product liability and calculation of damages. The scope of tort liability damages includes physical damage, property damage and mental damage. But there is special damage in product liability damages, which is punitive damage. Firstly, with the price of product increasing, whether the victims can receive the compensation for the defect product in the tort litigation becomes more and more important. Secondly, Tort Liability Law determined the scope of the physical damage, but there still remains some conflicts with other regulations. Finally, punitive damage is the characteristic in product liability legal system. This paper will study on the above matters and some related cases, and be used to the previous reference for comparative study of China and Korea product liability legal system.

A study on the inflammatory response induced by LPS of the Arthrospira platensis ethanol extract

Shi Jie Zhang,Jae-Chan Yang,Bo-Ae Kim 한국응용과학기술학회 (구.한국유화학회) 2019 한국응용과학기술학회지 Vol.36 No.3

Arthrospira platensis has been reported to contain a variety of substances such as phycocyanin, β-carotene, vitamin E and other carotenoids. In this study, zebrafish were treated with indoor cultivation spirulina ethanol extracts(ICAE) to determine toxicity(coagulation rate, hatching rate, heart rate). We used the DCFH-DA staining method to detect the effect of reactive oxygen species(ROS) generation on lipopolysaccharide(LPS)-induced zebrafish embryos ROS various concentrations(0.01, 0.05, 0.1, 0.5mg/ml) of ICAE. Cell toxicity was measured by WST-1 assay on RAW 264.7 macrophage cell line. Also, measured the inhibitory effect of nitric oxide(NO) and prostaglandin E₂(PGE₂) production in RAW264.7 macrophages induced by LPS at various concentrations of ICAE. The results of embryo coagulation rate, hatching rate, heart rate of zebrafish at various(0.01, 0.05, 0.1mg/ml) of ICAE was no toxicity. The ICAE treated group had an inhibitory effect on NO and PGE₂ production compared and decreased with concentration. The results of this study ethanol extract of Arthrospira platensis has an anti-inflammatory effect and suggest that is worthy of use cosmetics for skin protection.

A Study on Efficient Skeleton Retargeting Methods for Metahuman

Shi-Jie Sun,Ki-Hong Kim 한국콘텐츠학회 2023 한국콘텐츠학회 ICCC 논문집 Vol.2023 No.12

Analysis and Compensation Control of Dead-Time Effect on Space Vector PWM

Shi, Jie,Li, Shihua The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.2

Dead-time element must be set into space vector pulsed width modulation signals to avoid short circuits of the inverter. However, the dead-time element distorts the output voltage vector, which deteriorates the performance of electrical machine drive system. In this paper, dead-time effect and its compensation control strategy are analyzed. Based on the analysis, the voltage distortion caused by dead-time is regarded as two disturbances imposed on dq axes in the rotor reference frame, which degenerates the current tracking performance. To inhibit the adverse effect caused by the dead-time, a control scheme using two linear extended state observers is proposed. This method provides a strong ability to suppress dead-time effects. Simulations and experiments are conducted on a permanent magnet synchronous motor drive system to demonstrate the effectiveness of the proposed method.

Analysis and Compensation Control of Dead-Time Effect on Space Vector PWM

Jie Shi,Shihua Li 전력전자학회 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.2

Dead-time element must be set into space vector pulsed width modulation signals to avoid short circuits of the inverter. However, the dead-time element distorts the output voltage vector, which deteriorates the performance of electrical machine drive system. In this paper, dead-time effect and its compensation control strategy are analyzed. Based on the analysis, the voltage distortion caused by dead-time is regarded as two disturbances imposed on dq axes in the rotor reference frame, which degenerates the current tracking performance. To inhibit the adverse effect caused by the dead-time, a control scheme using two linear extended state observers is proposed. This method provides a strong ability to suppress dead-time effects. Simulations and experiments are conducted on a permanent magnet synchronous motor drive system to demonstrate the effectiveness of the proposed method.

The Progress of Research on Chinese Stabilized Fertilizer

Shi Yuanliang,Wang Lingli,Shi Xiaoyu,Li Jie,Sun Yi 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Effect of Tensile Deformation of Austenite on the Morphology and Strength of Lath Martensite

Zengmin Shi,Kai liu,Maoqiu Wang,Jie Shi,Han Dong,Jian Pu,Bo Chi,Yisheng Zhang,Li Jian 대한금속·재료학회 2012 METALS AND MATERIALS International Vol.18 No.2

A hot-rolled steel, 22SiMn2TiB, was employed to study the effect of austenite deformation on the micro-structure and strength of the subsequently formed lath martensite. It was revealed that the sizes of the mar-tensite packet, block and lath were refined by the tensile deformation of austenite at temperatures above 850 °C. With the increase of the deformation temperature, the packet size increased, whereas the block size decreased. The width of the lath was independent of the prior austenite grain size and the deformation temperature. The refinement of martensite blocks was considered to strengthen the ausformed martensite.

Nkd2, a negative regulator of Wnt pathway, delays mitotic exit in Hela cell

Yu-Jie Shi,Keke Huo 한국유전학회 2013 Genes & Genomics Vol.35 No.5

Frequent amplification and abundant expression of Nkd2 has been identified in malignant peripheral nerve sheath tumors (MPNSTs), dominant for genomic instability, who is involved in both Wnt pathway and EGFR signaling pathway. As a negative regulator of Wnt pathway, Nkd2 suppresses Wnt signaling by binding to Dvl1 and causing its ubiquitination followed by 26S proteasome degradation. On the other hand, it interacts with TGF-a for its transportation to basolateral plasma membrane in polarized epithelial cells. It is of interest to determine if Nkd2 over-expression contributes to tumorigenesis and genomic instablity. In this paper, we found that cells expressing NKD2 delayed mitotic exit stage after double thymidine block synchronization, but aneuploidy was not detected in these cells. This was further confirmed by Western blotting. In nocodazole-synchronised cells, Cyclin B1 degradation was delayed with Nkd2 overexpression compared to control group. Given many previous publications showed that Wnt pathway components areinvolved in mitotic progression. Further investigation on Nkd2’s function in mitosis might give more clues on MPNSTs pathological progression.