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Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication
Lingxia Li,Shengqing Li,Shengyi Han,Pengfei Li,Guoyu Du,Jinyan Wu,Xiaoan Cao,Youjun Shang 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.5
Background: Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells. Objectives: PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response. Methods: Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4+ and CD8+ T cells after PPRV infection. Results: PPRV stimulated the differentiation of CD4+ and CD8+ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation. Conclusions: This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.
Aloe-emodin suppresses prostate cancer by targeting the mTOR complex 2.
Liu, Kangdong,Park, Chanmi,Li, Shengqing,Lee, Ki Won,Liu, Haidan,He, Long,Soung, Nak Kyun,Ahn, Jong Seog,Bode, Ann M,Dong, Ziming,Kim, Bo Yeon,Dong, Zigang IRL Press] ; Oxford University Press 2012 Carcinogenesis Vol.33 No.7
<P>Phosphatidylinositol 3-kinase (PI3-K) amplification and phosphatase and tensin homolog (PTEN) deletion-caused Akt activation contribute to the development of prostate cancer. Mammalian target of rapamycin complex 2 (mTORC2) is a kinase complex comprised of mTOR, Rictor, mSin1, mLST8/G관L and PRR5 and functions in the phosphorylation of Akt at Ser473. Herein, we report that mTORC2 plays an important role in PC3 androgen refractory prostate cell proliferation and anchorage-independent growth. Aloe-emodin, a natural compound found in aloe, inhibited both proliferation and anchorage-independent growth of PC3 cells. Protein content analysis suggested that activation of the downstream substrates of mTORC2, Akt and PKC관, was inhibited by aloe-emodin treatment. Pull-down assay and in vitro kinase assay results indicated that aloe-emodin could bind with mTORC2 in cells and inhibit its kinase activity. Aloe-emodin also exhibited tumor suppression effects in vivo in an athymic nude mouse model. Collectively, our data suggest that mTORC2 plays an important role in prostate cancer development and aloe-emodin suppresses prostate cancer progression by targeting mTORC2.</P>
Single-Phase Virtual Synchronous Generator for Distributed Energy Resources Integration
Zeng, Zheng,Cheng, Chong,Tang, Shengqing,Yang, Huan,Zhao, Rongxiang Journal of International Conference on Electrical 2014 Journal of international Conference on Electrical Vol.3 No.3
Virtual synchronous generator (VSG) in single-phase to interface distributed renewable energy resources is investigated in this paper. Mathematical models and numerical analysis are utilized to illustrate the features of the VSG. Enhanced control strategy is presented to ensure the performance of the VSG. Besides, a second order generalized integer (SOGI) is employed to calculate the instantaneous output power of the VSG in virtual ${\alpha}{\beta}$ frame. By the means of a phase-locked loop based scheme, the VSG can seamlessly transform between islanded and grid-tied modes, which can meet the requirements of micro-grid. At last, the validation and the proposed approach are verified by the simulated results using PSCAD/EMTDC.
Selective harmonic active tuning control method for hybrid active power filters
Luo, Zhaoxu,Liu, Yang,Li, Shengqing,Luo, Qin The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.6
This paper proposes a selective harmonic active tuning control method for hybrid active power filters (HAPFs). A HAPF is composed of a voltage source inverter (VSI) and an LC filter. With this method, the VSI in the HAPF is controlled as a virtual capacitance that varies with the dominant harmonic frequencies. Thus, the LC filter can be forced to be tuning at these frequencies, which significantly improves the harmonic filtering characteristic of the LC filter. Meanwhile, to enhance the filtering performance in the case of a small power grid impedance or to damp the resonances between an LC filter and the grid impedance, the harmonics in the grid source current are detected and proportionally fed back to the controller, which brings about an effect equivalent to adding a series harmonic resistance on the grid source side. The proposed control method has the advantages of a simple control structure and easy implementation. Simulation and experimental results confirm the effectiveness and feasibility of the proposed method.
Xiaoyu Deng,Yanlong Cong,Renfu Yin,Guilian Yang,Chan Ding,Shengqing Yu,Xiufan Liu,Chun-feng Wang,Zhuang Ding 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.4
Characteristic clinical manifestations of Newcastle diseaseinclude leukopenia and immunosuppression. Peripheral bloodmononuclear cells (PBMCs) are the main targets of Newcastledisease virus (NDV) infection. To survey changes in proteomicexpression in chicken PBMCs following NDV infection,PBMC proteins from 30 chickens were separated using twodimensionalelectrophoresis (2-DE) and subjected to massspectrometry analysis. Quantitative intensity analysis showedthat the expression of 78 proteins increased more thantwo-fold. Thirty-five proteins exhibited consistent changes inexpression and 13 were identified as unique proteins bymatrix assisted laser desorption ionization-time of flight massspectrometer/mass spectrometer including three that weredown-regulated and 10 that were up-regulated. These proteinswere sorted into five groups based on function: macromolecularbiosynthesis, cytoskeleton organization, metabolism, stressresponses, and signal transduction. Furthermore, Westernblot analysis confirmed the down-regulation of integrin-linkedkinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of targetcells to NDV infection at the molecular level. Additionally,results from this study have helped elucidate the molecularpathogenesis of NDV and may facilitate the development ofnew antiviral therapies as well as innovative diagnostic methods.
Yingjie Sun,Pin Zhang,Hang Zheng,Luna Dong,Lei Tan,Cuiping Song,Xusheng Qiu,Ying Liao,Chunchun Meng,Shengqing Yu,Chan Ding 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.1
T-cell internal antigen-1 (TIA-1) has roles in regulating alternative pre-mRNA splicing, mRNA translation, and stress granule (SG) formation in human cells. As an evolutionarily conserved response to environmental stress, SGs have been reported in various species. However, SG formation in chicken cells and the role of chicken TIA-1 (cTIA-1) in SG assembly has not been elucidated. In the present study, we cloned cTIA-1 and showed that it facilitates the assembly of canonical SGs in both human and chicken cells. Overexpression of the chicken prion-related domain (cPRD) of cTIA-1 that bore an N-terminal green fluorescent protein (GFP) tag (pntGFP-cPRD) or Flag tag (pFlag-cPRD) induced the production of typical SGs. However, C-terminal GFP-tagged cPRD induced notably large cytoplasmic granules that were devoid of endogenous G3BP1 and remained stable when exposed to cycloheximide, indicating that these were not typical SGs, and that the pntGFP tag influences cPRD localization. Finally, endogenous cTIA-1 was recruited to SGs in chicken cells and tissues under environmental stress. Taken together, our study provide evidence that cTIA-1 has a role in canonical SG formation in chicken cells and tissues. Our results also indicate that cPRD is necessary for SG aggregation.
Platycladus orientalis PoKub3 is Involved in Abiotic Stress Responses in Transgenic Arabidopsis
Xiamei Yao,Jin Zhang,Jing Ji,Jianyun Yue,Tiantian Xie,Nan Deng,Jianfeng Liu,Shengqing Shi,Zeping Jiang,Ermei Chang 한국식물학회 2017 Journal of Plant Biology Vol.60 No.4
Ku70-binding proteins associate with Ku70 and their expression levels can affect DSB repair efficiency via the DNA-PK-dependent repair pathway. However, how Ku70-binding proteins in plants exert a regulatory function under abiotic stress is poorly understood. Here, we cloned and characterized a PoKub3 gene from 500-year-old Platycladus orientalis. With increasing age, PoKub3 expression in P. orientalis increased gradually. The PoKub3 expression levels in leaves were upregulated under salt, heat, UV-C and abscisic acid treatments according to qRT-PCR. Moreover, PoKub3 overexpression in Arabidopsis thaliana improved tolerance to salt and drought stress compared with wild-type (WT) and vector control (VC) plants. High RAB18 and DREB2A expression and low JAZ1 and ABI2 expression provided strong evidence that salt tolerance was enhanced in the overexpression plants. Similarly, high RAB18 and DREB2A expression, accompanied by low JAZ1 and LOX1 expression and high DREB1A, CPK10, GSTF6 and APX1 expression, suggested the drought tolerance mechanism was associated with the abscisic acid pathway. In addition, lower malondialdehyde content, electrolyte leakage and stomatal conductance, and higher soluble sugar and relative water contents in PoKub3 overexpression lines than in WT and VC plants demonstrated its role in salt and drought tolerance. Together, these findings show that PoKub3 positively regulates salt and drought tolerance by regulating stress-related genes.