The Effect of Carbon Nanomaterials on Senescence of Cut Flowers in Carnation (Dianthus caryophyllus L.)

Di Zhang,Xiaotong Chen,Jiangyuan Sheng,Yafei Zhang,Xiaohui Shen,Li Ren 한국원예학회 2021 원예과학기술지 Vol.39 No.3

Carbon nanomaterials (CNMs) have remarkable chemical, physical, electrical, and structural properties and favorable biocompatibility. This study used carnation (Dianthus caryophyllus L.) cut flowers as a model to evaluate the protective effects of 3 kinds of CNMs (single-walled carbon nanotubes [SWCNT], graphene quantum dots [GQD], and fullerenes [C60]) on the antioxidant activity and senescence of plant cells. We found that 1 mg·L<SUP>-1</SUP> C60 and 25 mg·L<SUP>-1</SUP> GQD extended the vase life (VL) of carnation by approximately 10%. SWCNT cannot be absorbed and transported by plant vascular tissue, and higher concentrations of SWCNT can block vascular tissue, leading to decreased VL. Physiological tests have shown that the The malondialdehyde (MDA) and hydroxyl radical (OH·) levels significantly decreased after the GQD and C60 treatments, and the main factors that cause cell damage changed from H₂O₂ to OH·. The in vitro Fenton reaction and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay indicated that both C60 and GQD may inhibit OH· generation by approximately 10% to 15%, and GQD had higher DPPH free radical scavenging activity. Accordingly, a suitable concentration of C60 and GQD can influence reactive oxygen species metabolism and downstream biological events, including the cell redox state, the antioxidant system, and membrane lipid peroxidation, effectively delaying senescence and abscission of plant tissue.

Sliding Mode-based Predictive Congestion Control in a Satellite Space Information Transmission Network

Sheng Liu,Di Wu,Lan-Yong Zhang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.8

A satellite information transmission network is different from a ground network. The link distance between the satellite and the ground is large, and the communication between them is affected by the ionosphere. The ground congestion control algorithm cannot provide fast and stable responses to short-term burst flows. Therefore, aiming at the problem of large propagation delays and the complex environments of satellite-to-ground links, this paper investigates the Vegas congestion mechanism of the Space Communication Protocol Standard Transport Protocol (SCPS-TP), incorporates the interference affecting satellite communication links into a discrete model, combines interference estimation with controller design, proposes a reaching law and sliding mode-based predictive control algorithm for satellite communication, and suggests the stability of the introduced algorithm. The simulation results demonstrate the anti-jamming ability of the proposed algorithm for addressing short-term burst flows, and the prediction mechanism of the algorithm improves the utilization of satellite network bandwidth.

Effect of Agricultural Land Use Change on Community Composition of Bacteria and Ammonia Oxidizers

Rong Sheng,Delong Meng,Minna Wu,Hongjie Di,Hongling Qin,Wenxue Wei 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Continuous Spatio-Temporal Representation in Media Art

Ma Di,Zhao Yuan Sheng,Hyung Gi Kim 중앙대학교 영상콘텐츠융합연구소 2018 TechArt :Journal of Arts and Imaging Science Vol.5 No.2

Involvement of FoxM1 in Non-Small Cell Lung Cancer Recurrence

Xu, Nuo,Wu, Sheng-Di,Wang, Hao,Wang, Qun,Bai, Chun-Xue Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Background: Predictive biomarkers for lung cancer recurrence after curative tumor resection remain unclear. This study set out to assess the role of FoxM1 in the recurrence of non-small cell lung cancer. Methods: Immunohistochemistry for FoxM1 expression was performed on paraffin-embedded tumor tissues from 165 NSCLC patients. Association of FoxM1 expression with clinicopathological parameters and disease free survival were evaluated. Results: Our results indicated FoxM1 expression to be significantly associated with poorer tissue differentiation (P =0.03), higher TNM stage (P <0.01), lymph node metastasis (P <0.01), advanced tumor stage (P <0.01), and poorer disease free survival (P <0.01). Multivariable analysis showed that FoxM1 expression increased the hazard of recurrence (hazard ratio= 1.96, 95% CI, 1.04-3.17, P <0.05), indicating that FoxM1 is an independent and significant predictor of lung cancer recurrence. Conclusion: Therefore, FoxM1 is an independent risk factor for recurrence of NSCLC. Elevated FoxM1 expression could be used as an indicator of poor disease free survival.

Analysis of differentially expressed proteins between the spinetoram-susceptible and -resistant strains of Plutella xylostella (L.)

Fei Yin,Qing Sheng Lin,Xia Feng,Huan-yu Chen,Zhen-yu Li,Zhen-di Hu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

Plutella xylostella (L.), aworldwide vegetable pest, has developed resistance to spinetoram,whichwas previously effective for the control of P. xylostella (L.). The insecticidal resistance mechanismis essential to develop effective resistance management strategies. To explore the spinetoram resistance mechanism, a comparative proteomics approach was used to investigate the proteomic differences between the spinetoram-susceptible strain (SS) and spinetoram-resistant strain (RS) of P. xylostella (L.). Approximately 280 protein spotswere detected on each SDSPAGE gel. Of these, 19 proteinswere successfully identified byMALDI-TOF-MS.·Therewere 6 significantly downregulated spots and 13 up-regulated spots in RS, which showed significantly difference compared to that in SS. Based on the gene ontology(GO) system and KEGG database, the 19 identified proteins were classified into 6 groups includingmetabolisms, signal transduction, chaperones, transcriptional, protein synthesis, structural protein. Meanwhile, the expression profiles of 5 resistant related protein were further analysed by qRT-PCR. The results showed that 60S acidic ribosomal protein P2, glutathione S-transferase isozyme 3 and glutathione Stransferase deltawere significantly up-regulated,while phosphoglyceratemutase and receptor for activated protein kinase C homolog were significantly down-regulated. The expression tendency of mRNA was in accordance with which of protein. This study provided evidences that spinetoram induces proteomic changes in P. xylostella (L.), and it is contributed to help us understand the resistance mechanism of P. xylostella (L.) to spinetoram.

Prediction of rolling bearing performance degradation based on sae and TCNattention models

Yaping Wang,Dekang Hou,Di Xu,Sheng Zhang,Chaonan Yang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.4

A single feature cannot show the operational state of a bearing during its entire life cycle. Therefore, a rolling bearing performance deterioration prediction method based on an SAE and the TCN-attention model is proposed. The SAE method is used to fuse the timedomain indicator and the frequency-domain indicator to construct the performance degradation characteristic indicator. The evaluation indices are used to comprehensively evaluate multiple performance degradation indices, and the fused feature indices together, to filter out the features that have a good overall performance. Attention is added to the TCN model, and the output state weight of the TCN model is calculated through a scoring function to increase the important information weight and the prediction accuracy. The appropriate network structure and parameter configuration are determined, and the rolling bearing performance degradation prediction model is established. A validation is performed using publicly available datasets from the University of Cincinnati and XJTU-SY. The results show that the method is more sensitive to the critical information part of the long time series than the other models. At the same time, the average absolute error and the root mean square error are minimized, the accuracy of the rolling bearing performance degradation prediction is high, and the model has a strong robustness and generalization abilities. Additionally, the model has practical engineering value for predicting the health status of equipment.

MTHFR Polymorphisms and Pancreatic Cancer Risk:Lack of Evidence from a Meta-analysis

Li, Lei,Wu, Sheng-Di,Wang, Ji-Yao,Shen, Xi-Zhong,Jiang, Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: Methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms have been reported to be associated with pancreatic cancer, but the published studies had yielded inconsistent results.We therefore performed the present meta-analysis. Methods: A search of Google scholar, PubMed, Cochrane Library and CNKI databases before April 2012 was conducted to summarize associations of MTHFR polymorphisms with pancreatic cancer risk. Assessment was with odds ratios (ORs) and 95% confidence intervals (CIs). Publication bias were also calculated. Results: Four relative studies on MTHFR gene polymorphisms (C667T and A1298C) were involved in this meta-analysis. Overall, C667T(TT vs. CC : OR = 1.61, 95%CI = 0.78 - 3.34; TT vs. CT : OR = 1.41, 95%CI = 0.88-2.25; dominant model: OR = 0.68, 95%CI = 0.40-1.17; recessive model: OR = 0.82, 95%CI = 0.52-1.30) and A1298C(CC vs. AA:OR=1.01, 95%CI=0.47-2.17; CC vs. AC: OR=0.99,95%CI=0.46-2.14; dominant model: OR=1.01, 95%CI = 0.47-2.20; recessive model: OR = 1.01, 95%CI = 0.80-1.26) did not increase pancreatic cancer risk. Conclusion: This meta-analysis indicated that MTHFR polymorphisms (C667T and A1298C) were not associated with pancreatic cancer risk.

Role of NEAT1/MiR-9-5p/SLC26A2 Pathway on Human Airway Smooth Muscle Cell

Xiangying Wang,Ruju Xu,Di Chi,Chufeng Dai,Meiling Sheng 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.9

Purpose: Asthma is a serious inflammatory disease of the respiratory system in which airway smooth muscle cells (ASMCs) playa key role. This study aimed to investigate the expression of SLC26A2 in human ASMCs (HASMCs) and the regulatory mechanismof SLC26A2 in the proliferation and inflammatory factor production of HASMCs. Materials and Methods: We obtained the asthma-associated differential mRNA SLC26A2 by bioinformatics analysis in childhoodacute asthma samples. To investigate its role in airway inflammation and airway remodeling, we treated HASMCs with plateletderivedgrowth factor (PDGF) in an in vitro model and determined SLC26A2 expression in cells using western blotting. Cell proliferationwas detected by MTT and EdU assays, and cell contractile phenotype marker proteins were measured. Cell migrationand production of inflammatory factors were determined by Transwell and ELISA assays. Additionally, the upstream regulatorymiRNA and LncRNA of SLC26A2 were identified by bioinformatics, luciferase reporter gene, and RIP analyses. Results: SLC26A2 was significantly upregulated in bioinformatics analysis of pediatric asthma-related sample. PDGF treatmentup-regulated SLC26A2 expression in HASMCs, whereas the knockdown of SLC26A2 inhibited PDGF-stimulated proliferation,migration, and production of inflammatory factors, and enhanced the expression of cell contractile phenotype marker proteinsin HASMCs. Luciferase reporter and RIP experiments validated that NEAT1 targeted miR-9-5p to regulate SLC26A2, thereby influencingthe biological function of PDGF-induced HASMCs. Conclusion: These findings indicate that NEAT1-mediated miR-9-5p targeting of SLC26A2 inhibits the PDGF-induced proliferationand production of inflammatory factors in HASMCs. These findings highlight potential therapeutic targets for asthma andairway inflammation.

The Relative Influence of Diet and Physical Activity on Obesity in China

Cui Zhao-Hui,Li Yan-Ping,Di Yu-Feng,Ba Lei,Hu Xiaoqi,Ma Guan-Sheng The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.3

The purpose of this study is to investigate the relative influence of diet and physical activity on obesity. The subjects were 155 adults aged 35-52 years from 24 neighborhood committees in 4 urban districts of Beijing (male : 78, female : 77). They were divided into normal weight, overweight and obese groups according to their BMI. The general information of the subjects was collected by interview-administered questionnaire. Dietary intake was obtained by three-day(two weekdays and one weekend day) food weighted method, physical activity was assessed by a validated combination of data obtained from activity monitors, bicycling information and activity records. There were no significant differences of age, gender, height, educational, family economic level, smoking and drinking between different groups. The proportion of flour intake was higher in obese group compared to normal weight and overweight groups, and that of vegetables is lower in obese group. The physical activity (PAL) was not significantly different between two groups of the normal, overweight and obese groups. After the adjustment for confounding factors using logistic regression model, we found that the proportion of flour intake was positively associated with obesity, while the proportion of vegetable intake was inversely associated with obesity. It is concluded that dietary patterns were associated with obesity and diets composed of more vegetables and less staple combined with physical activities could contribute to obesity prevention.