Preparation of Dopamine Transporter-specific Antibodies Using Molecular Cloned Genes

Lee, Shee Yong,Im, Suhn Young,Kim, Kyeong Man 전남대학교 약품개발연구소 1999 약품개발연구지 Vol.8 No.1

Dopamine transporter (DAT) plays the most important role in terminating the actions of dopamines released into the synaptic deft DAT is also the target of various psychotropic drugs such as cocaine and amphetamine. In this study we prepared DAT specific antibodies using the 2nd extracellular loop of rat DAT as an antigen. The 2nd extracellular loop of the rat DAT was expressed in bacteria as a fusion protein with glutathione-S-transferase, and injected into rabbits to raise antibodies. Produced antibodies dearly recognized the rat DAT in ELISA, immunoblotting, and immunoprecipitation. As expected from the high sequence homology between the rat and human DAT, the antibodies raised for the rat DAT cross-reacted with the human DAT in the immunoblotting. Considering die specificity for DAT with wide range of applications such as ELISA, immunoblotting, and immunoprecipitation, these antibodies would be valuable tool for understanding the pharmacological actions of dopamine transporter and drug addiction.

Preparation of Dopamine Transporter-specific Antibodies Using Molecular Cloned Genes

Lee, Shee-Yong,Im, Suhn-Young,Kim, Kyeong-Man The Pharmaceutical Society of Korea 1999 Archives of Pharmacal Research Vol.22 No.3

Dopamine transporter (DAT) plays the most important role in terminating the actions of dopamines released into the synaptic cleft. DAT is also the target of various psychotropic drugs such as cocaine and amphetamine. In this study were prepared DAT-specific antibodies using the 2nd extracellular loop of rat DAT as an antigen. The 2nd extracellular loop of the rat DAT was expressed in bacterial as a fusion protein with glutathione-S-transferase, and injected ito rabbits to raise antibodies. Produced antibodies clearly recognized the rat DAT in ELISA, immunoblotting, and immumoprecipitation. As expected from the high sequence homology between the rat and human DAT, the antibodies raised for the rat DAT cross-reacted with the human DAT in the immunoblotting. Considering the specificity for DAT with wide range of applications such as ELISA, immunoblotting, and immunoprecipitation, these antibodies would be valuable tool for understanding the pharmacological actions of dopamine transporter and drug addition.

Effects of Panax ginseng on Morphine-induced Immune Suppression

Kim, Kyeong Man,Kim, Young Ran,Lee, Shee Yong,Kim, Ae Young 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.3

To investigate the possibility of Panax ginseng as a therapeutic agent for the immune suppression, ginseng total saponin (GTS) extracted from korean red ginseng was tested on immune functions from morphine-induced immune suppressed mice. To study how immune functions are affected by morphine and also to test whether GTS can be an useful therapeutic agent for morphine toxicity, several parameters were employed, body weight, immune organ weight, B cell functions, and T cell function. Morphine impaired the development of body weight and immune organ weight such as spleen and thymus. Morphine also depressed a B-cell function, antibody production. T-cell functions studied by type IV hypersensitivity test were most markedly affected by morphine treatment. GTS restored most of morphine-induced immune suppression. GTS restored the morphine-induced decrease in spleen weight to body weight ratio in a dose dependent manner, but not the body weight decrease. Also all of the morphine-induced impairments of B cell functions and cellmediated immunity were fully recovered by GTS. These results suggest that ginseng product could be very helpful for the treatment of immune suppression occurring in morphine abusers.

우리나라 은행사업의 효율성:Fourier Flexible 비용함수의 분석을 중심으로

정운찬,함시창,김규한,정지만 한국경제학회 2000 經濟學硏究 Vol.48 No.1

본 연구는 우리나라 은행산업의 효율성을 살펴보기 위해 규모의 경제 및 범위의 경제 여부를 분석하였다 이때 분석에 사용된 비용함수로는 자료의 전 구간에서 유연성이 있는 Fourier flexible 함수형태를 중심으로 분석하였으며, 이들 결과를 트랜스로그 비용함수를 이용한 결과와 비교하였다. 또한 은행산업에서의 산출물과 투입물에 대한 견해차를 감안하여 6개 모형을 설정하였으며, 엄밀한 자료분석을 위해 은행계정의 대차대조표 및 손익계산서뿐만 아니라 신탁계정에 관한 대차대조표 및 손익계산서를 각 은행별로 분석하였다. 실증분석 결과, 분석모형에 따라 다소의 차이는 있으나, 우리나라 은행산업에는 규모의 경제가 있는 것이 발견되었다. 특히 기존 연구와는 달리 소형은행보다는 오히려 대형은행에서 규모의 경제 가능성이 높게 제시되었다. 한편, 범위의 경제 여부는 분석모형에 따라 차이는 있으나 대체로 업무다각화에 따른 비용절감의 효과가 있는 것으로 나타났다.

우리나라 증권산업의 효율성 분석 : Fourier Flexible 비용함수의 분석을 중심으로 Based on Fourier Flexible Cost Function

정운찬,함시창,김규한,정지만 한국금융학회 2000 金融學會誌 Vol.5 No.1

본 연구는 우리나라 증권산업의 규모와 범위의 경제, 그리고 X-비효율성 분석에 대한 연구이다. 이를 위하여 기존의 Translog 함수형태대신 Fourier 함수형태를 기초로 여러 다양한 규모와 범위의 경제지표를 사용하고, DFA 방식(Distribution Free Approach)으로 X-비효율성을 계산하려 했다는 것이 본 논문의 주요 특징이 되겠다. 본 연구의 연구결과는 다음과 같이 정리된다. 첫째, 실증분석 결과 우리나라 증권산업 전체에 상당한 수준의 규모의 경제가 존재하고 있음을 발견하였다. 둘째, 우리나라 증권산업의 경우 범위의 경제가 존재하는 것으로 나타나고 있다. 셋째, Fourier 함수결과를 기준할 경우 기업별 X-효율성은 증권회사규모가 증가함에 따라 규칙적으로 증가하는 것으로 보인다. 결국 본 연구의 분석결과는 우리나라 증권산업의 규모와 범위의 경제성을 높이고 효율성을 향상시키기 위해서는 증권회사의 규모를 대형화하는 것이 필요함을 보여주는 좋은 예가 된다고 하겠다.

Panax ginseng blocks morphine-induced thymic apoptosis by lowering plasma corticosterone level

Kim, Young Ran,Lee, Shee Yong,Shin, Boo Ahn,Kim, Kyeong Man 전남대학교 약품개발연구소 1999 약품개발연구지 Vol.8 No.1

The effects of Panax ginseng on morphine-induced immune suppression were studied. Morphine (20 ㎎/㎏, SC, 4 days) decreased body weight increment rate and caused atrophy of thymus and spleen. These changes were partly reversed by concomitant administration of ginseng total saponin (GTS, 100 ㎎/㎏, oral, 9 days). Morphine elevated the serum corticosterone level and caused the DNA fragmentation of thymocytes. These sequential events were completely blocked by a concomitant administration of GTS. Flow cytometry analysis showed that GTS specifically blocked morphine-induced apoptosis of thymocytes.

Effects of Panax ginseng on Type I Hypersensitivity

Kim, Young-Ran,Lee, Eun,Lee, Shee-Yong,Kim, Kyeong-Man The Korean Society of Ginseng 1996 Journal of Ginseng Research Vol.20 No.1

Effects of Panax ginseng on allergic reactions were studied uslng various in vivo and in vitro experimental models such as 48-hr passive cutaneous anaphylaxis, mediators-induced skin reactions, histamine release from rat peritoneal mast cells, hexosaminidase release from RBL-2H3 cells, and lipoxygenase assay . In all of anti-allergic experiments we conducted, ginseng components (50% ethanol extract or ginseng total saponin or ginsenosides) extracted from Korean red ginseng, did not show significant anti-allergic actions. In 48-hr passive cutaneous anaphylaxis and mediators-induced skin reactions, 50% ethanol extract did not suppress hypersensitivity reactions. Total saponin, 50% ethanol extract, and 8 major ginsenosides did not show inhibitory effects on lipoxygeanse activity. Ginseng total saponin did not inhibit histamine release from rat peritoneal mast cells. All of the ginseng components mentioned above were also tested on RBL-2H3 cells, but none of them inhibited hexosaminidase release from this cell line. These results suggest that Panax ginseng does not have effects on allergic reactions at the level of 50% ethanol extract or total saponin used. All of 8 major saponin components tested ($Rb_1$, $Rb_2$, Rc, Rd, Re, Rf, $Rg_1$, $Rg_2$), did not inhibit lipoxygenase activity and degranulation events.

A Study on the Effects of an Extended CT Field of View (FOV) on the Standardized Uptake Value (SUV) in a PET/CT scan Using 18F-fluoro-2deoxy-D-glucose

박순기,남기표,정우영,김경식,신상기,Shee-Man Cho,김호성,동경래,박용순,정운관,조재환,여화연 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.12

This study examined the effect of an extended CT field of view (FOV) on the standardized uptake value (SUV) of Positron emission tomography - computed tomography (PET/CT) when image extends beyond the CT FOV. CT images were reconstructed at different FOV sizes (500 and 700 mm). Two sets of CT images were reconstructed from the CT projection data using two FOV sizes. Twenty patients were examined in this study. The PET images were reconstructed from attenuation maps with a 500 mm CT FOV and a 700 mm extended CT FOV images. The regions of interest (ROIs) were drawn on the PET images. In addition, in the twenty patients, the PET images reconstructed by 500 mm CT FOV and 700 mm extended CT FOV were compared with the SUVmax. When attenuation maps with the 700 mm extended CT FOV were used, the SUVmax analyses of the liver (p = 0.000), lung (p = 0.007) and mediastinum (p = 0.001) were statistically significant. A 700 mm extended CT FOV helps recover the true activity distribution in PET emission data, and affects the SUV measurements of the liver, lung and mediastinum.

총 계수 분석법과 평균 계수 분석법에 따른 신장 기능의 차이 분석

정우영,조시만,박승온,심동오,JUNG WOO-YOUNG,CHO SHEE-MAN,PARK SEUNG-YONG,SHIM DONG-OH 대한방사선사협회 2003 대한방사선사협회지 Vol.29 No.1

감마카메라를 이용하여 영상화할 수 있는 $^{99m}Tc-DMSA$ 신장 검사는 비침습적으로 신장의 기능과 형태를 동시에 평가할 수 있고, 신장 기능의 이상은 형태학적인 변화 보다 먼저 나타나기 때문에 다른 검사보다 $^{99m}Tc-DMSA$ 신장 검사를 이용하면 병변을 조기에 진단할 수 있는 장점이 있다. 신장의 상대적 기능 평가시에 깊이 보정, 총 계수 또는 평균 계수의 사용, BKG 보정, SPECT 검사의 Purpose : $^{99m}Tc-DMSA$ renal scan can be used in the evaluation of relative renal function and some anatomy. The relative renal function can be assess by measurement of $^{99m}Tc-DMSA$ uptake by the individual kidney. Renal counts

병원 내 방사선 작업종사자의 만성적 방사선 피폭에 의한 혈액학적 성분 변화에 관한 연구

류재광,정우영,신상기,조시만,동경래,김현수,Ryu, Jae-Kwang,Jung, Woo-Young,Shin, Sang-Ki,Cho, Shee-Man,Dong, Kyung-Rae,Kim, Hyun-Soo 대한핵의학기술학회 2008 핵의학 기술 Vol.12 No.2

본 연구는 의료기관에 종사하는 의료방사선 작업종사자에게서 직업적 방사선 피폭으로 인한 인체 내 생리적인 변화를 통해 만성적 저선량 방사선피폭의 위험도를 예측하며 현 수준의 변화 상태를 파악하기 위한 목적으로 서울시내 일개 대형병원에서 최근 1~9년간 방사선 작업종사자로 근무한 370명을 대상으로 남녀 간의 체내 혈액성분의 수치변화를 대조군과의 비교 분석을 실시하여 다음과 같은 결론을 얻었다. 1. 종사자의 1~9년간 평균 방사선 누적선량은 남자가 $9.65{\pm}15.2\;mSv$, 여자가 $4.89{\pm}5.55\;mSv$로 남자에서 높았으며 이는 통계적으로도 유의하였다(p<0.01). 2. 남녀 종사자 모두에서 누적선량과 종사기간의 차이에 따른 검사항목들과의 상관성은 매우 미약하였다(r<${\pm}0.25$) 3. 종사기간이 증가할수록 남자에서는 백혈구의 감소자와 증가자, 혈색소의 감소자, 당질의 증가자 비율 유의하게 높았으며(p<0.05), 여자에서도 백혈구의 감소 및 증가자, 호산구의 감소자의 비율이 유의하게 높았다(p<0.01). 4. 누적선량의 증가할수록 남녀모두에서 오히려 이상분포를 보이는 항목은 줄어들었으며, 남자는 림프구와 혈소판의 감소자가 유의하게 높았고(p<0.05) 여자는 림프구와 적혈구의 감소뿐만 아니라 종사기간에서 이상을 보였던 백혈구의 감소 및 증가자 역시 유의한 증가를 나타냈다(p<0.05). 5. 전체적인 변화를 보면 남자 종사자에서는 혈액성분 중 적혈구, 혈소판, 림프구가 주로 감소되며 백혈구는 감소와 증가양상을 같이 보였다. 화학성분 중 당질의 증가가 유의했지만 큰 의미는 없었다. 여자 종사군에서는 남자와 똑같게 백혈구가 감소, 증가 양상을 동시에 보였으며 그밖에 적혈구와 림프구, 호산구의 감소가 유의했다. 이상의 결과를 종합해보면 비록 낮은 선량에 피폭이 되고 있는 의료방사선 종사자일지라도 방사선 피폭에 있어서는 자유롭지 못하여 그 영향으로 인한 체내 생물학적 변화를 겪을 수 있으며 그 변화는 선량에 의존하기 보다는 다분히 확률적일 수 있다는 사실이다. 그러므로 항상 미연에 피폭을 방지하기 위한 노력을 종사자 스스로 기울임을 물론 관련 당국 또한 종사자들의 건강을 체계적으로 관리할 수 있는 시스템을 하루빨리 구축하여 방사선 피폭으로 인한 직업병의 발생의 우려를 없애야 할 것으로 사료된다. Purpose: This prospective study was conducted to reveal the haematological index change by low level radiation exposure in radiological environment our hospital workers. Materials and Method: We gathered the cumulative dose by Thermoluminenscent Dosimeters (TLD) over 9-yr period and examined hematological index counts change (RBC, Hb, Platelet, WBC, Monocyte, Lymphocyte, Neutrophilic, Basophilic, Eosinophilic) both occupational workers and controls. Of a total 370 occupational workers and 335 controls were compared. Results: This analysis has led to the following general observations 1) The average cumulative dose in male and female were $9.65{\pm}15.2\;mSv$, $4.82{\pm}5.55\;mSv$ respectively. 2) In both male and female, there were very low relationship between occupation period and cumulative dose (r< ${\pm}0.25$). 3) Occupation period was more increased, in male, WBC counts decreased and increased workers, RBC counts decreased workers were more than controls group (p<0.05). In female, WBC counts decreased and increased workers and W-eosino counts decreased workers were more than controls group (p<0.01). 4) Cumulative dose was more increased, in male, W-Lympho counts decreased workers and Platelet counts deceased workers were more than controls group (p<0.05). In female, W-lympho counts decreased workers and RBC counts decreased workers were more than controls group (p<0.05). Conclusions: We can find some kinds of blood index abnormal distribution in occupational radiation workers by comparing with controls. Occupational workers cannot avoid radiation exposure, in spite of the control it. Actually low level radiation adverse effect occurred not dose but probability. So workers must always try to reduce exposure by ourselves, furthermore as long as possible the government should provide rapidly that national system on radiation control for worker's health.