Association of rs10757274 and rs2383206 Polymorphisms on 9p21 locus with Coronary Artery Disease in Turkish Population

Çağrı Yayla,Kaan Okyay,Asife Şahinarslan,Akın Yılmaz,Atiye Seda Yar Sağlam,Azmi Eyiol,Hasan Ata Bolayır,Burak Sezenöz,Sevda Menevşe,Atiye Çengel 대한심장학회 2016 Korean Circulation Journal Vol.46 No.5

Background and Objectives: Genetic predisposition is an important risk factor for coronary artery disease (CAD). In this study, we aimed to evaluate the impact of rs10757274 and rs2383206 polymorphisms in chromosome 9p21 on presence and severity of CAD in a Turkish population. Subjects and Methods: A total of 646 patients who underwent coronary angiography were included in this study. Coronary vessel score and Gensini score were calculated to assess the angiographic severity of CAD. Alleles of AA, AG, and GG were determined for rs10757274 (polymorphism-1) and rs2383206 (polymorphism-2) polymorphisms located in chromosome 9p21 from the blood samples. Results: There was a significant difference between the alleles in polymorphism-1 in the presence of coronary artery disease (38.9% in AA, 48.0% in GG and 56.4% in AG, p=0.017). However, there was no difference between the alleles in polymorphism-2. According to vessel scores, there was a significant difference between the alleles in polymorphism-1 (AA 0.71±1.04, GG 0.88±1.07, AG 1.06±1.12, p=0.018). In polymorphism-2, vessel scores did not show a difference between the alleles. In polymorphism-1, there was a significant difference in Gensini score (p=0.041). Gensini scores did not differ between the alleles in polymorphism-2 (p>0.05 for all). In multivariate analyses, none of the alleles was an independent factor for presence of CAD. Conclusion: The presence of rs10757274 polymorphism including AG allele in chromosome 9p21 was related to CAD. However, this relationship was not independent of other cardiovascular risk factors.

Shear deformation model for reinforced concrete columns

Sezen, Halil Techno-Press 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.28 No.1

Column shear failures observed during recent earthquakes and experimental data indicate that shear deformations are typically associated with the amount of transverse reinforcement, column aspect ratio, axial load, and a few other parameters. It was shown that in some columns shear displacements can be significantly large, especially after flexural yielding. In this paper, a piecewise linear model is developed to predict an envelope of the cyclic shear response including the shear displacement and corresponding strength predictions at the first shear cracking, peak strength, onset of lateral strength degradation, and loss of axial-load-carrying capacity. Part of the proposed model is developed using the analysis results from the Modified Compression Field Theory (MCFT). The results from the proposed model, which uses simplified equations, are compared with the column test data.

Investigations on Bacteria as a Potential Biological Control Agent of Summer Chafer, Amphimallon solstitiale L. (Coleoptera: Scarabaeidae)

Sezen Kazlm,Demir Ismail,Katl Hatice,Demirbag Zihni The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.5

Studying the bacteria of hazardous insects allows the opportunity to find potentially better biological control agents. Therefore, in this study, bacteria from summer chafer (Amphimallon solstitiale L., Coleoptera: Scarabaeidae) we isolated and identified the insecticidal effects of bacteria isolated from A. solstitiale and Melolontha melolontha L. (common cockchafer, Coleoptera: Scarabaeidae) and the mixtures of these bacterial isolates were investigated on A. solstitiale larvae. Crystals from Bacillus sp. isolated from M. melolontha were also purified, and tested against the second and third-stage larvae of A. solstitiale. The bacterial isolates of A. solstitiale were identified as Pseudomonas sp., Pseudomonas sp., Bacillus cereus and Micrococcus luteus, based on their morphology, spore formation, nutritional features, and physiological and biochemical characteristics. The insecticidal effects of the bacterial isolates determined on the larvae of A. solstitiale were $90\%$ with B. cereus isolated from A. solstitiale, and $75\%$ with B. cereus, B. sphaericus and B. thuringiensis isolated from M. melolontha within ten days. The highest insecticidal effects of the mixed infections on the larvae of A. solstitiale were $100\%$ both with B. cereus+B. sphaericus and with B. cereus+B. thuringiensis. In the crystal protein bioassays, the highest insecticidal effect was $65\%$ with crystals of B. thuringiensis and B. sphaericus isolated from M. melolontha within seven days. Finally, our results showed that the mixed infections could be utilized as microbial control agents, as they have a $100\%$ insecticidal effect on the larvae of A. solstitiale.

Determination of Thermo-Electrical Properties in Sn Based Alloys

Sezen Aksöz,Yavuz Ocak,Kâzlm Keslioslu,Necmettin Mara ll 대한금속·재료학회 2010 METALS AND MATERIALS International Vol.16 No.3

The variation of thermal conductivity of solid phase versus temperature for Sn-21 wt.% Bi, Sn-25 wt.% In and Sn-35 wt.% In-26 wt.% Bi alloys were measured with a radial heat flow apparatus. From the graphs of thermal conductivity versus temperature, the thermal conductivity of the solid phases at their melting temperatures and the thermal temperature coefficients for the same alloys were obtained. The ratios of thermal conductivity of liquid phase to solid phase for the same materials were measured with a Bridgman type directional solidification apparatus. The variations of electrical conductivity of solid phases versus temperature for the same alloys were determined from the Wiedemann-Franz law by using the measured values of thermal conductivity. From the graphs of electrical conductivity versus temperature, the electrical temperature coefficients for the same alloys were also determined. According to present experimental results it can be concluded that the thermal and electrical conductivity of Sn based alloys depend on the thermal and electrical conductivity of the alloying elements. If the thermal and electrical conductivity of the alloying elements are lower than the thermal conductivity of Sn, the thermal conductivity of Sn based alloys decreases, whereas, otherwise,it increases.

Shear deformation model for reinforced concrete columns

Halil Sezen 국제구조공학회 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.28 No.1

Column shear failures observed during recent earthquakes and experimental data indicate that shear deformations are typically associated with the amount of transverse reinforcement, column aspect ratio, axial load, and a few other parameters. It was shown that in some columns shear displacements can be significantly large, especially after flexural yielding. In this paper, a piecewise linear model is developed to predict an envelope of the cyclic shear response including the shear displacement and corresponding strength predictions at the first shear cracking, peak strength, onset of lateral strength degradation, and loss of axial-load-carrying capacity. Part of the proposed model is developed using the analysis results from the Modified Compression Field Theory (MCFT). The results from the proposed model, which uses simplified equations, are compared with the column test data.

Towards ambient pressure for scanning photoelectron imaging and spectromicroscopy

Hikmet SEZEN,Matteo AMATI,Luca GREGORATTI 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Investigations on Bacteria as a Potential Biological Control Agent of Summer Chafer, Amphimallon solstitiale L. (Coleoptera: Scarabaeidae)

Kazlm Sezen,Ismail Demir,Hatice Katl,Zihni Demirbag 한국미생물학회 2005 The journal of microbiology Vol.43 No.5

Studying the bacteria of hazardous insects allows the opportunity to find potentially better biological control agents. Therefore, in this study, bacteria from summer chafer (Amphimallon solstitiale L., Coleoptera: Scarabaeidae) we isolated and identified the insecticidal effects of bacteria isolated from A. solstitiale and Melolontha melolontha L. (common cockchafer, Coleoptera: Scarabaeidae) and themixtures of these bacterial isolates were investigated on A. solstitiale larvae. Crystals from Bacillus sp.isolated from M. melolontha were also purified, and tested against the second and third-stage larvae of A. solstitiale. The bacterial isolates of A. solstitiale were identified as Pseudomonas sp., seudomonas sp., Bacillus cereus and Micrococcus luteus, based on their morphology, spore formation, nutritional features, and physiological and biochemical characteristics. The insecticidal effects of the bacterial isolates determined on the larvae of A. solstitiale were 90% with B. cereus isolated from A. solstitiale, and 75% with B. cereus, B. sphaericus and B. thuringiensis isolated from M. melolontha within ten days. The highest insecticidal effects of the mixed infections on the larvae of A. solstitiale were 100% both with B. cereus+B. sphaericus and with B. cereus+B. thuringiensis. In the crystal protein bioassays, the highest insecticidal effect was 65% with crystals of B. thuringiensis and B. sphaericus isolated from M. melolontha within seven days. Finally, our results showed that the mixed infections could be utilized as microbial control agents, as they have a 100% insecticidal effect on the larvae of A. solstitiale.

Effects of Lycopene Alone or Combined with Melatonin on Methotrexate-Induced Nephrotoxicity in Rats

Oguz, Elif,Kocarslan, Sezen,Tabur, Suzan,Sezen, Hatice,Yilmaz, Zehra,Aksoy, Nurten Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Methotrexate (Mtx), used for its anticancer and immunsuppresive properties, is known to be a nephrotoxic agent. We aimed to investigate the effects of lycopene (Lyc) alone or combined with melatonin (Mel) on Mtxinduced nephrotoxicity since both of these agents have antioxidant and anti-inflammatory effects. Nephrotoxicity was induced by intraperitoneal administration of methotrexate at a dose of 20 mg/kg. Treatment both with Lyc alone and Lyc combined with Mel provided significant reduction in tumor necrosis factor-alpha, interleukin 1-beta and ceruloplasmin levels in Mtx administered rats. Hovewer, Lyc combined with Mel provided a significant reduction also in NO levels. Hstopathological examination showed that there was an obvious improvement in the degenerative changes compared to Mtx administrated group with the Lyc combined Mel group giving best protection. In conclusion Lyc alone and combined with Mel provided significant improvement against renal damage caused by Mtx, preseumably via antioxidant and anti-inflammatory activities.

The First Study on Bacterial Flora and Biological Control Agent of Anoplus roboris (Sufr., Coleoptera)

Demirbag, Zihni,Sezen, Kazim,Demir, Ismail The Microbiological Society of Korea 2002 The journal of microbiology Vol.40 No.2

The hazelnut leaf holer (Anoplus roboris Sufr,, Coleoptera: Curculionidae) is a devastating pest of hazelnut and oak trees. It causes approximately 20-30% economic damage to hazelnut production per year in Turkey. In the present study, in order to fond a more effective and safe biological control agent against A. roboris, we investigated the bacterial flora of the hazelnut leave holer, and tested them for insecticidal effects on it. According to morphological, physiological and biochemical tests bacterial flora were identified as Bacillus circulans (Ar1), Bacillus polymyxa (Ar2), Enterobacter sp. (Ar3) and Bacillus sphaelicus (Ar4). Insecticidal effects of bacterial isolates were performed on adult A. roboris. The highest insecticidal effect determined was 67% by B. sphaericus within eight days. The insecticidal effects of the other isolates (Ar1, Ar2 and Ar3) were determined as 33%, 47% and 47% within the same period, respectively.

A Highly Pathogenic Strain of Bacillus thuringiensis serovar kurstaki in Lepidopteran Pests

Kati, Hatice,Sezen, Kazim,Nalcacioglu, Remziye,Demirbag, Zihni The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.6

In order to detect and identify the most toxic Bacillus thuringiensis strains against pests, we isolated a B. thuringiensis strain (Bn1) from Balaninus nucum (Coleoptera: Curculionidae), the most damaging hazelnut pest. Bn1 was characterized via morphological, biochemical, and molecular techniques. The isolate was serotyped, and the results showed that Bn1 was the B. thuringiensis serovar, kurstaki (H3abc). The scanning electron microscopy indicated that Bn1 has crystals with cubic and bipyramidal shapes. The Polymerase Chain Reactions (PCRs) revealed the presence of the cry1 and cry2 genes. The presence of Cry1 and Cry2 proteins in the Bn1 isolate was confirmed via SDS-PAGE, at approximately 130 kDa and 65 kDa, respectively. The bioassays conducted to determine the insecticidal activity of the Bn1 isolate were conducted with four distinct insects, using spore-crystal mixtures. We noted that Bn1 has higher toxicity as compared with the standard B. thuringiensis subsp. kurstaki (HD-1). The highest observed mortality was 90% against Malacosoma neustria and Lymantria dispar larvae. Our results show that the B. thuringiensis isolate (Bn1) may prove valuable as a significant microbial control agent against lepidopteran pests.