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Prostaglandin F2α Controls Reactive Oxygen Species in Bovine Corpus Luteum
Seunghyung Lee,Boo-Keun Yang,Choon-Keun Park 한국동물번식학회 2015 Reproductive & developmental biology Vol.39 No.1
Luteolysis is a cyclical regression of the corpus luteum in many non-primate mammalian species. Prostaglandin F2α (PGF2α) from the uterus and ovary induces functional and structural luteolysis in bovine. The action of PGF2α is mediated by PGF2α receptor located on the luteal steroidogenic and endothelial cell membranes. PGF2α plays an important role in regulating nitric oxide production in endothelial cells of the bovine corpus luteum. Nitric oxide production and nitric oxide synthase activity are stimulated and induced by PGF2α in luteal endothelial cells. Moreover, the reactive oxygen species inhibits progesterone secretion in bovine luteal cells and induces apoptosis. Thus, the interaction between PGF2α and reactive oxygen species provides important aspects in physiology of the corpus luteum forfunctional and structural luteolysis.
RalA-binding Protein 1 is an Important Regulator of Tumor Angiogenesis
Seunghyung Lee(이승형) 한국생명과학회 2014 생명과학회지 Vol.24 No.5
본 논문은 RLIP76 단백질이 암, 종양 혈관 신생 및 그 치료에 미치는 중요성을 보고함에 있다. 암의 연구에 있어서, 종양의 혈관 신생을 억제시키는 인자와 영향을 끼치는 인자를 밝혀내는 것은 암의 억제와 치료를 위한 분자 생물학적 기전에 중대한 영향을 미친다. 최근 연구에서, RLIP76 단백질이 혈관 신생에 영향을 끼치는 역할을 발견하였다. RLIP76 제거 마우스의 종양은 일반 종양과 비교하여 혈관의 크기가 작으며, 가늘고, 그 혈관의 수가 적고 길이가 짧은 것으로 보고되고 있다. 게다가, Matrigel plugs을 이용한 혈관 신생 실험에서, RLIP76이 제거된 마우스에서는 혈관 생성이 억제 되었으나, 일반 마우스에서는 혈관이 생성되었다. 또한, 혈관세포를 이용한 in vitro 실험에 있어서, proliferation, migration 및 cord formation 모두가 RLIP76에 의해서 조절되었다. 일반적으로 RLIP76은 대부분의 인간 조직과 종양에서 발현되며, 약의 저항 기전 연구에 이용되고 있기도 한다. 또한, 이 RLIP76은 small GTPase R-Ras와 상호작용을 통하여 세포 spreading 및 migration에 관여하고 있다. 이러한 결과는 RLIP76와 암 연구의 중요성을 보고하고 있으며, 혈관 세포의 기능의 기전 및 종양의 혈관 신생을 위한 RLIP76단백질의 중요성을 알리고 있고, RLIP76의 추가적인 연구를 통하여 종양의 혈관 신생의 기전을 밝히는 것이 필요함을 제안하는 바이다. Tumor angiogenesis is important in tumorigenesis and therapeutic interventions in cancer. To know inhibitor and effector of tumor angiogenesis in cancer, the specific gene of tumor and angiogenesis may develop the mechanisms of cancer suppression and therapy. Recently, we described the role of RalA-binding protein 1 (RLIP76) in tumor angiogenesis. Tumor vascular volumes were diminished, and vessels were fewer in number, shorter, and narrower in RLIP76 knockout mice than in wild-type mice. Moreover, angiogenesis in basement membrane matrix plugs was blunted in the knockout mice in the absence of tumor cells, with endothelial cells isolated from the lungs of these animals exhibiting defects in migration, proliferation, and cord formation in vitro. RLIP76 is expressed in most human tissues and is overexpressed in many tumor types. In addition, the protein regulates tumorigenesis and angiogenesis in vivo and in vitro. As the export of chemotherapy agents is a prominent cellular function of RLIP76, it is a major factor in mechanisms of drug resistance. Moreover, RLIP76 acts as a selective effector of the small GTPase, R-Ras, and regulates R-Ras signaling, leading to cell spreading and migration. Furthermore, in skin carcinogenesis, RLIP76 knockout mice are resistant, with tumors that form showing diminished angiogenesis. Thus, RLIP76 is required for efficient endothelial cell function and angiogenesis in solid tumors.
Prostaglandin F2α Controls Reactive Oxygen Species in Bovine Corpus Luteum
Seunghyung Lee,Boo-Keun Yang,Choon-Keun Park 한국동물생명공학회(구 한국동물번식학회) 2015 Reproductive & developmental biology Vol.39 No.1
Luteolysis is a cyclical regression of the corpus luteum in many non-primate mammalian species. Prostaglandin F2α (PGF2α) from the uterus and ovary induces functional and structural luteolysis in bovine. The action of PGF2α is mediated by PGF2α receptor located on the luteal steroidogenic and endothelial cell membranes. PGF2α plays an important role in regulating nitric oxide production in endothelial cells of the bovine corpus luteum. Nitric oxide production and nitric oxide synthase activity are stimulated and induced by PGF2α in luteal endothelial cells. Moreover, the reactive oxygen species inhibits progesterone secretion in bovine luteal cells and induces apoptosis. Thus, the interaction between PGF2α and reactive oxygen species provides important aspects in physiology of the corpus luteum forfunctional and structural luteolysis.
The Molecular Functions of RalBP1 in Lung Cancer
Seunghyung Lee 대한의생명과학회 2014 Biomedical Science Letters Vol.20 No.2
RalBP1 is an ATP-dependent non-ABC transporter, responsible for the major transport function in many cells including many cancer cell lines, causing efflux of glutathione-electrophile conjugates of both endogenous metabolites and environmental toxins. RalBP1 is expressed in most human tissues, and is over-expressed in non-small cell lung cancer cell lines and in many other tumor types. Blockade of RalBP1 by various approaches has been shown to increase sensitivity to radiation and chemotherapeutic drugs, leading to cell apoptosis. In xenograft tumor models in mice, RalBP1 blockade or depletion results in complete and sustained regression across many cancer cell types including lung cancer cells. In addition to its transport function, RalBP1 has many other cellular and physiological functions, based on its domain structure which includes a unique Ral-binding domain and a RhoGAP catalytic domain, as well as docking sites for multiple signaling proteins. Additionally, RalBP1 is also important for stromal cell function in tumors, as it was recently shown to be required for efficient endothelial cell function and angiogenesis in solid tumors. In this review, we discuss the cellular and physiological functions of RalBP1 in normal and lung cancer cells.
Seunghyung Lee,Hee-Woo Park,Hee-Tae Cheong,Choon-Keun Park,Boo-Keun Yang 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.1
The purpose of this study was to examine the effects of taurine and vitamin E on sperm characteristics damaged by bromopropane (BP) in pig. We evaluated toxicity of BP on viability, membrane integrity and mitochondrial activity of spermatozoa. 1-BP (0, 2.5, 5.0, 10, and 50 μM), 2-BP (0, 2.5, 5.0, 10, and 50 μM), taurine (0, 5.0, 10, and 25 μM) and vitamin E (0, 50, 100, and 200 μM) were treated in fresh boar semen for 6 h. 10 and 50 μM of 1-BP and 2-BP inhibited sperm viability, membrane integrity and mitochondrial activity in fresh boar semen (P<0.05). 25 μM of taurine increased sperm viability and membrane integrity (P<0.05), 100 μM of vitamin E enhanced viability and mitochondrial activity of sperm (P<0.05). Finally, 10 μM of 1-BP and 2-BP was co-treated with taurine (25 μM) and vitamin E (100 μM) in the fresh boar semen. The co-treated samples did affected viability, membrane integrity and mitochondrial activity of sperm. In conclusion, taurine and vitamin E can improve and maintain sperm quality in fresh boar semen.
클라우드-네이티브 IoT-Cloud 서비스를 위한 마이크로서비스 구조 기반의 서비스 합성 설계 및 검증
이승형(Seunghyung Lee),한정수(Jungsu Han),권진철(JinCheol Kwon),김종원(JongWon Kim) 한국정보과학회 2020 정보과학회 컴퓨팅의 실제 논문지 Vol.26 No.3
클라우드, 사물인터넷, 인공지능 기술의 발달과 함께 서비스를 효율적으로 개발하고 검증하기 위해 기존의 모놀리식 방식의 서비스 합성에서 컨테이너 중심의 클라우드-네이티브 컴퓨팅을 기반으로 개발하는 서비스 합성으로 진화하고 있다. 즉, 서비스를 마이크로서비스 구조에 맞게 나누어 소 기능들로 개발하고, 컨테이너 형태로 구성하는 서비스 합성을 통해 온전한 서비스를 만드는 방법이 중요해지고 있다. 특히 사물인터넷과 클라우드가 융합된 형태인 IoT-Cloud 서비스들은 클라우드, 사물인터넷, 인공지능의 역할을 수행하는 여러 기능들을 통해 구성되기 때문에 마이크로서비스 구조 방식의 서비스 합성이 적합하다. 본 논문에서는 클라우드-네이티브 컴퓨팅 환경에서 IoT-Cloud 서비스를 개발하고 합성하는 방안을 세부적으로 구상한다. 또한 본문에서 제시한 서비스 합성 방안을 통해 개발한 특정 서비스를 두 개의 실제 서비스 환경에 적용하여 이식성을 검증하고, 이로써 마이크로서비스 구조 기반 서비스의 유용성을 보인다. As the cloud, Internet of Things (IoT), and Artificial Intelligence (AI) technologies develop, the demand for services that use these technologies is exploding. In recent years, to develop and verify services quickly and efficiently, the previous monolithic service development method is evolving toward a service composition method based on container-based microservices architecture (MSA). Especially, IoT-Cloud services, which are combined with the IoT and the cloud, are a chain of various functions that play the role of IoT, Cloud, and AI. Thus, the service composition method is suitable for developing and operating IoT-Cloud service based on microservices architecture. In this paper, we designed a way to develop and compose the intelligent IoT-Cloud service in the cloudnative computing environment based on the IoT-Cloud pattern. Additionally, through the proposed service composition method, we applied specific IoT-Cloud services to two different real test bed environments to validate mobility and usefulness. This validation demonstrates that the cloud-native services can be partially adapted to the service environment and operate flexibly.