경식도 심초음파도의 Direct Planimetry를 이용하여 측정한 대동맥판 면적의 심박출량 및 좌심실 구출율의 변화에 따른 정확도

진승원,김종진,김희열,박지원,전두수,윤호중,박준철,노태호,채장성,김재형,홍순조,최규보 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

이온토포레시스에 의한 경피 투과도 조절

이승연,여지선,김혜지,민혜란,오승열 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.21 No.-

We have prepared hydrophilic karaya gum patches containing ketoprofen and studied some important factors which affect the transdermal flux of ketoprofen, as a first step to develop an iontophoretic transdermal patch system. The effect of penetration enhancer (propylene glycol monolaurate: PGML), short-time current treatment and continuous current on flux was studied. The effect of pH of the receptor solution and poly (L-lysine) (PLL) was also studied. PGML increased the passive flux markedly, and as the concentration of PGML in the matrix increased, passive flux increased. Iontophoretic treatment of skin (0.4 mA/cm²) for an hour increased the average passive flux more than two times, when the matrix contained no enhancer. It also increased the average passive flux further, when enhancer was incorporated into the matrix. Continuous iontophoretic transport showed unexpected result; the average flux at 0.2 mA/cm² was similar to that at 0.4 mA/cm², and was higher than that at 0.6 mA/cm². PLL increased the continuous iontophoretic transport at all current density, and the average flux was higher at 0.6 mA/cm² than at 0.4 and 0.2 mA/cm². Similar results were obtained when the receptor solution was changed to pH 4.0 buffer solution. These result demonstrate, that electro-osmotic flow is playing an important role in the flux of ketoprofen.

환경오염원으로서 Benzoate에 노출된 Acinetobacter sp. KS-1의 생존과 형태변화

송승열,오계헌 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

The purpose of this work was to investigate the survival and morphological changes of Acinetobacter sp. KS-1 when exposed by benzoate as an environmental stressor. The strain KS-1 was isolated from environmental samples around hospitals and could utilize benzoate as the sole source of carbon and energy. Strain KS-1 was examined the physiological and biological characteristics. Physiological analysis using BIOLOG GN2 MicroPlate system was performed to identify the strain, which could be assigned to genus Acinetobacter and designated as Acinetobacter sp. KS-1. Microscopic examination of the strain revealed Gram-negative and short rod cells. Survival of Acinetobacter sp. KS-1 cells was examined in different concentrations of benzoate. Survival of the KS-1 cell had less effect on the concentrations of 10 mM, 100 mM, or 500 mM of benzoate, whereas significant decrease of survival rates was measured at 1 M benzoate. Scanning electron microscopy was used to examine the morphological changes of Acinetobacter sp. KS-1 cells depending on the different concentrations of benzoate. However little morphological changes of the cells were observed in this experiments.

여드름 환자에서의 경구 레티노이드(Roaccutane^(�))의 치료 효과

김종율,김성진,이지범,이승철,원영호 全南大醫大 2000 전남의대학술지 Vol.36 No.2

Chitosan이 사과 겹무늬썩음병균 Botryosphaeria dothidea의 생육에 미치는 영향

이승지,엄재열,이용현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

고분자물질에 의한 사과 겹무늬썩음병 방제법을 개발함에 있어서 chitosan을 코팅용 소재로 활용하기 위하여, 사과 겹무늬썩음병균인 Botryosphaeria dothidea에 대한 Chitosan의 항진균활성을 고체배양 및 액체배양 조건하에서 검토하였다. Chitosan은 고체배양시 B. dothidea의 균사생장억제, 자라난 균사의 응집, 균사의 팽윤 및 세포의 미세구조의 변화를 유발하였다. 액체배양실험 결과 chitosan은 낮은 농도에서도 강력한 균체생육 억제효과를 보였으며, 1.0mg/ml의 첨가농도에서 90% 이상의 억제효과를 나타내었다. 또한 균사의 신장과정에 영향을 미쳐 균사체의 응집현상이 관찰되었다. 또한 chitosan이 포자의 발아를 지연시켰을 뿐만 아니라 발아관의 형태적 변화를 유발시켰다. 단량체인 glucosamine은 glucosamine은 B. dothidea의 생육저해를 유발하지 않았으며, 항진균 활성은 chitosan polymer에 의한 것임을 알았다. To examine the potential utilization of chitosan, the biodegradable natural ploymer, as a control agent of apple white rot caused by Botryosphaeria dothidea in a new control measure by coating it on the diseased branches, the various antifungal activites of chitosan was investigated. Chitosan showed significant inhibitory effect on the mycelial growth of B. dothidea, along with the morphological changes including hyphal swelling and ultrastructural changes on solid PDA medium. In liquid PD broth medium, the chitosan showed more significant effect on the growth of B. dothidea also forming cell clusters indicating affection on the hyphal extension. The growth of B. dothidea was inhibited more than 90% at the concentration of 1.0 mg/ml. Chitosan also detained the spore germination and induced the morphological change of germ tubes. Glucosamine, monomer of chitosan, did not affect on the growth of B. dothidea indicating the antifungal activity was caused by chitosan polymer.

치성감염에 의해 발생된 후측인두부 및 종격동 농양

이승호,정종철,김건중,정주성,유선열,박문성,김창룡 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

Odontogenic infections are usually locally confined, self-limiting processes. However, under certain circumstances, they may break through the bony, muscular, and mucosal barriers and spread into contiguous fascial spaces or planes far from the initial site of involvement, resulting in severn life-threatening complications, such as retropharyngeal spread, suppurative mediastinal extension, airway obstruction, pleuropulmonary suppuration, and hematogenous dissemination to distant organs. The mortality arte for mediastinitis from odontogenic infection ranges from 40% to 60%. Therefore rapid evalution and treatment is essential with a combination of life support, antibiotic therapy, and surgical intervention. Recently, we experienced three cases of retropharyngeal and mediastinal abscesses secondary to odonogenic infections. In all patients, early diagnosis was possible by CT scanning and physical examination. The prognosis was good in all patients by using urgent aggressive surgical and antibiotic therapy.

소독제 과산화수소에 노출된 Legionella pneumophila의 생존력

송승열,오계헌 순천향대학교 기초과학연구소 2000 순천향자연과학연구 논문집 Vol.6 No.2

Survival of L. pneumophila exposed by hydrogen peroxide as a disinfectant was monitored. Initially morphological and physiological characteristics were examined. The strain was small Gram-negative cocco-bacillus and could not degrade carbohydrate. Survival of L. pneumophila was examined in the buffered charcoal yeast extract medium containing 0-5% hydrogen peroxide. The survival rates of cells decreased with increasing concentrations of hydrogen peroxide. The growth of L. pneumophila was significantly inhibited by the exposure of hydrogen peroxide. When the strain was treated with >0.01% hydrogen peroxide, the cells could not grow on the medium. Survival rate of cells decreased when trated with >0.01% of concentrations of hydrogen peroxide. Time-survival studies showed that exposure of L. pneumophila cells to 0.3% hydrogen peroxide resulted in at least 80% killing after 90 min and not detected the survivors at 120 min. No cells were grown at the concentrations of 1%> hydrogen peroxide. As the result, hydrogen peroxide suggested a good candidate for the control agent of L. pneumophila.

Iontophoresis 를 이용한 NADPH 의 피부투과 및피부자극

이승연,오승열 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.20 No.-

Nicotinamide Adenine Dinucleotide Phosphate (NADPH) has antioxidant effect in the body and thus decreases the symptoms of senility. The delivery of NADPH through skin is a formidable challenge due to its physical and chemical properties (such as rapid decomposition by oxidation, low partition coefficient in stratum corneum, high molecular weight and charge). The purpose of this work is to prepare a hydrogel patch system containing NADPH, and to study its in-vitro iontophoretic transport through hairless mouse skin. We have investigated two factors (concentration of NADPH and current density) that can affect the iontophoretic flux. The stability of NADPH in aqueous solution and the possible skin irritation by iontophoretic delivery of NADPH was also investigated. The results showed that, compared to passive flux, the total amount of NADPH transported increased by the application of cathodal current. Anodal flux was similar to passive flux. Flux increase was proportional to the concentration of NADPH in the donor solution and to the current density. Nearly 50 % of NADPH degraded during the experimental period in aqueous solution. The results of primary irritatation test showed no erythema or edema after iontophorsis. These results suggest that therapeutic amount of NADPH can be delivered through skin using iontophoresis, without any development of erythema or edema.

ISO 9000 시리즈를 이용한 대학평가시스템

황승국,우정열,강성수 경남대학교 공업기술연구소 2001 硏究論文集 Vol.19 No.-

본 논문의 목적은 ISO 9000 시리즈를 이용하여 대학평가시스템을 개발하는 것이다. 여기서는 품질 기능전개의 각 단계에서의 평가방법의 개념을 사용한다. 대학에서의 교육행정과 평가영역에 대한 가중치는 주어진다. 개발된 평가시스템을 사용하여 대학평가시스템 현재수준에 대한 평가점수를 얻을 수 있다. 이 방법은 대학평가시스템의 운영과 개선에 도움을 줄 것이다. 수치 예의 설명을 통하여 이 방법의 효과를 보인다. The purpose of this paper is to develop an university evaluation system using ISO 9000 Series. Here, we use the concept from the evaluation method in each stage of Quality Function Deployment. The weights for education and administration and the evaluation sector in university was given. Using the evaluation system developed, we can acquire the evaluated score for the present level of the university quality system. This method will help to manage and improve the university quality system. We show the efficiency of this method by illustrating numerical example.

Benzoate에서 배양된 Acinetobacter baumannii로부터 Catechol 1,2-dixoygenase의 정제와 촉매적 성질

송승열,오계헌 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.2

The purpose of this work was to perform the catalytic properties and sequence analysis of catechol 1,2-dioxygenase (C1,2O) purified from Acinetobacter baumannii which was grown on benzoate as a carbon source. C1,20 demonstrated its enzyme activity to other substrates, catechol and 4-methylcatechol. The optimum temperature of C1,20 was 35℃, and the optimal pH was in the range from pH 7.5 to 9. Ag^+, Hg^+, and Cu^+2 showed inhibitory effect on the activity of C1,20. Molecular weight of the enzyme was determined to approximately 36 kDa by SDS-PAGE. N-terminal amino acid sequence of C1,20 was analysed as ^1MNYQQIDALVKQMNVDTAKG^20 and exhibited 95% sequence homology with that of C1,20 from Acinetobacter radioresistens. For internal sequencing analysis, trypsin digestion and peptide mapping were performed. Molecular weights of three digested peptide fragments were analyzed as 966.3 dalton, 1933.8 dalton and 2081.7 dalton by MALDI-TOF, which were matched with internal sequences (^1SQSDFNLRR^9, ^1TIEGPLYVAGAPESVGFAR^19, ^1HGNRPSHVHYFVSAPGYR^18)of Acinetobacter radioresistens.