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Stable Constitution of Artificial Oil Body for the Refolding of IGF1
Seung Phill Choi,장호남 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.2
Over-expression of oleosin-fused IGF1 results in the formation of insoluble aggregates in Escherichia coli occupying 35% of total proteins. In this study, a method based on artificial oil body (AOB) was applied to obtain active IGF1, insulin-like growth factor 1, from its insoluble form in one step. The stability of AOB emulsions constituted with soybean oleosin was maximized in the optimal composition of TAG (97.04%, wt/wt), PL (1.14%, wt/wt), and oleosin-UbIGF1 (1.82%, wt/wt) at pH 7.5 and at 25°C. Upon sonication, the mixture comprising plant oil and the insoluble fusion protein was readily assembled into AOBs. After peptide cleavage mediated by endopeptidase, the IGF1 free of fusion tags was liberated and then recovered. Subsequently, IGF1 self-refolded on AOB was obtained with high yield of 63.2 mg/g dry cell. This on-AOB refolding can be applied to the development of bacterial expression and purification of other active recombinant proteins
GO-10 : Poor prognosis after conservative surgery in stage I mucinous epithelial ovarian cancer
( Phill Seung Jung ),( Shin Wha Lee ),( Jeong Yeol Park ),( Dai Shik Suh ),( Dae Yeon Kim ),( Jong Hyeok Kim ),( Yong Man Kim ),( Young Tak Kim ),( Joo Hyun Nam ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: To evaluate the oncologic safety and to identify prognostic factor for recurrence in premenopausal women with stage I mucinous epithelial ovarian cancer (mEOC) who underwent conservative surgery. 방법: We enrolled 97 patients who were 1) premenopausal at the time of surgery and 2) confirmed to be the FIGO stage I. The surgical procedure of conservative surgery was unilateral salpingo-oophorectomy with/without contralateral ovarian wedge resection. 결과: The median age was 33 (range: 13-50) years at the time of surgery. Sixty-three (64.9%) patients were stage Ia, and 34 (35.1%) were Ic. Fifty-three (54.6%) patients underwent conservative surgery, and adjuvant chemotherapy was administrated to 61 (62.9%) patients. During 73.7 (range: 7.1-243.5) months of the median follow-up duration, 13 (13.4%) patients recurred and 8 (8.2%) of them died of disease. Among patients who underwent conservative surgery, there were 10 recurrences and the majority of recurred site was intraperitoneal cavity (n=8) with median age of 25 (range: 14-46) and median PFS of 17.8 (range: 5.1-53.3) months. In multivariate analysis, a significantly poorer prognosis was noted in patients who underwent conservative surgery (HR: 6.26, 95% CI: 1.53-25.53, p=0.011) and in patients with high preoperative CA-125 (HR: 1.98, 95% CI: 1.26-3.11, p=0.003). In patients with high preoperative CA-125 (>35U/mL, n=48), conservative surgery caused significantly higher recurrence rate (HR: 5.73, 95% CI: 1.22-27.03, p=0.027). 5-year disease-free survival rate was significantly lower in patients who underwent conservative surgery than in patients who did not (77.7% vs. 94.2%, p=0.047). 결론: In conclusion, conservative surgery might cause the poor prognosis in premenopausal women with stage I mEOCs, particularly with high preoperative CA-125 level. The further multicenter study with larger cohort would be needed to verify the oncologic safety of conservative surgery in mEOCs.
Case Report : A case report of prenatally diagnosed tetrasomy 18p
( Phill Seung Jung ),( Hye Sung Won ),( In Ji Cho ),( Min Kyung Hyun ),( Jae Yoon Shim ),( Pil Ryang Lee ),( Ahm Kim ) 대한산부인과학회 2013 Obstetrics & Gynecology Science Vol.56 No.3
Tetrasomy 18p, one of the most commonly observed isochromosomes, consists of two copies of the p arms on chromosome 18[i(18p)]. It is known as a de novo occurrence of non-disjunction or centromeric mis-division during meiosis II in the vast majority of cases. It has a prevalence of 1/140,000-180,000 live births and affects both genders equally. A 28-year-old woman was referred at 33+2 weeks gestation to rule out fetal congenital heart disease. Her prenatal ultrasonography showed intrauterine growth retardation, cardiomegaly, and imperforate anus. Doppler ultrasonographic finding showed fetal anemia. Tetrasomy 18p was confirmed by conventional karyotyping and fluorescence in situ hybridization. Because of its very low prevalence rate, only several cases of tetrasomy 18p has been reported worldwide and it has not yet been reported in Korea before. Therefore, we report a case of prenatally diagnosed tetrasomy 18p.
( Phill Seung Jung ),( Yeon Sik Na ),( Shin Wha Lee ),( Jeong Yeol Park ),( Jong Hyeok Kim ),( Young Man Kim ),( Joo Hyun Nam ),( Young Tak Kim ),( Dae Yeon Kim ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-
To correlate pathologic findings on conization specimens and other clinical parameters with residual disease in radical hysterectomy specimens in patients with cervical cancer to determine which subgroup of patients may be eligible for less radical surgery Between 2003 and 2011, we perfomed 472 conizations before treating cervical cancer patients. The exclusion criteria were the patients who were given neoadjuvant chemotherapy and/or definitive CCRT and/or radical trachelectomy and the patients whose pathologic reports were missing or unclear. A total of 292 patients who underwent radical hysterectomy after conization were included in this retrospective study. Mean age of this patients were 51.7 years (28-85) and mean size of residual tumor was 1.3cm (0-7.3 cm). FIGO stage distribution were 17 patients in IA1, 21 in IA2, 233 in IB1, 10 in IB2 and 11 in others. At radical hysterectomy, 87 patients (30%) had no residual cancer, while 205 patients (70%) had residual cancer. Univariate analysis showed that positive resection margin and tumor size more than 2cm were significant factors for predicting residual disease. These factors also significant in a multivariate analyisis (positive resection margin OR 6.869, 95% CI 2.734-17.262, p<0.001; tumor size >2cm OR 3.090, 95% CI 1.744-5.474, p<0.001). Of the resection margin status, Positive endocervical and deep margin were significant but not significant in exocervical margin. The patients with negative resection margin and small tumor (< 1 cm) had no residual disease and no recurrences in the median FU of 32 months. Positive resection margin on conization and large tumor size (>2c m) were significant factors for predicting residual cancer after radical hysterectomy. Using these factors, we can develop the predicting models for detecting subgroup of patients who are eligible for less radical surgery.
( Phill Seung Jung ),( Shin Wha Lee ),( Dae Yeon Kim ),( Jong Hyeok Kim ),( Yong Man Kim ),( Young Tak Kim ),( Joo Hyun Nam ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: The aim of this study is to evaluate diagnostic accuracy of combined 18F-fluoro-2-deoxyglucose-positron emission tomography/computed tomography (18F-FDG-PET/CT) imaging in suspected recurrence of epithelial ovarian cancer with non-disseminated lesions by correlating with pathologic report after secondary cytoreduction. 방법: We retrospectively reviewed patients with recurrent epithelial ovarian cancer after primary treatment at Asan Medical Center from January, 2004 to December, 2013. Among those, 130 patients underwent secondary cytoreduction after imaging studies such as combined 18F-FDG-PET/CT or CT. We evaluated the sensitivity and positive predictive value (PPV) of combined 18F-FDG-PET/CT imaging and compared with those of CT correlating with the pathologic result of the secondary cytoreduction. 결과: A total of 63 patients with a median age of 46 (range: 21-68) were enrolled in this study. 46 patients underwent combined 18F-FDG-PET/CT and 61 did CT imaging before their secondary cytoreduction. The distribution of the cell type is as followed; 44 papillary serous adenocarcinomas, 5 clear cell carcinomas, 6 serous carcinomas, 2 mucinous adenocarcinomas, 3 endometrioid adenocarcinomas, 3 other types. The median level of CA-125 before the secondary cytoreduction was 22.3 (range: 2.4-1380.0) U/mL, and the mean max standardized uptake value of 18F-FDG-PET in the study cohort was 6.2 (range: 1.6-26.7). After the secondary cytoreduction, 54 (85.7%) patients were confirmed to have recurred with a median maximal tumor diameter of 3.5 (range: 0.1-10.0) cm on the pathologic report. Combined 18F-FDG-PET/CT showed a sensitivity of 70.4% and a PPV of 82.6% with false positive rate of 17.4% in detecting recurrent epithelial ovarian cancer. 결론: Our study demonstrated a discrepancy from previous studies in respect of a diagnosis value of combined 18F-FDG-PET/CT imaging in recurrent epithelial ovarian cancers with non-disseminated lesions. A prospective study with larger cohort would be needed.