동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

초음파 지팡이를 위한 보행 장애물 검출에 관한 연구

양승열,함광근,허웅,강면규 明知大學校 産業技術硏究所 1995 産業技術硏究所論文集 Vol.14 No.-

In this paper, we study about obstacle detection system of the ultrarsonic crane for the blind walking. We sense a restrictive object by FMCW method for forward direction and pulse method for other direction. The system is composed of hardware system and software system. The hardware system consists of FMCW unit pu1se, unit control unit, and sound generation unit. The software system consists of front direction measuring routine, 1eft/right measuring routine, lower up/down routine. We develope the u1trasonic distanice sensor to measur the distance between 50㎝ and 500㎝. We obtained very accurate result for hard material and obtain satisfactory results for the dressed The system detected up to 5m for hard surface material and up to 2m the man. This system produce a beep sound forthe blind of which frequency corresponds to the obstacle distance.

제2형 당뇨병 동물모델인 OLETF 쥐에서 장기간 라미프릴 투여가 내당능 및 췌도 베타세포에 미치는 효과

고승현,윤건호,김명미,안유배,송기호,유순집,손현식,차봉연,이광우,손호영,강성구 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:최근 소개된 HOPE(Heart Outcomes Prevention Evaluation)study의 결과에 의하면, 심혈관질환이나 이에 대한 위험인자가 1가지 이상인 9,541명을 대상으로 안지오텐신 전환효소 억제제의 일종인 라미프릴을 투여하여 사망율과 뇌졸증, 심부전, 당뇨병 합병증이 개선되었을 뿐 아니라 다른 항고혈압제에 비해 새롭게 당뇨병으로 진단되는 환자가 줄었음을 보고한바 있다. 이에 저자들은 라미프릴(ramipril)을 제2형 당뇨병 동물모델에 장기간 투여함으로써 당뇨병을 예방하거나 고혈당 상태 또는 인슐린 감수성을 개선시킬수 있는지를 알아보고자 하였다. 방법:생후 24주된 체중 400∼450g의 OLETF와 LETO 쥐를 대상으로 6개월간 라미프릴 경구투여 하였고 혈압, 24시간 단백뇨 및 인슐린 내성검사를 시행하였다. 6개월 이후로는 8주간 투여군과 대조군의 체중을 비슷하게 맞추어 내당능 상태를 평가하였고 이후 다시 8주간 고농도 포도당을 투여하였다. 경구당부하검사를 시행한 후 희생시켜 조직표본을 만들어 guinea pig anti­human insulin 항체로 염색후 DAB로 발색하여 point count 법으로 베타세포를 정량하였고, 면역염색으로 췌도의 변화와 섬유화의 정도를 관찰하였다. 결과:라미프릴을 6개월간 투여하였을 때 OLETF 쥐에서 1)체중 증가가 적었으며 2)경구 당부하검사시 포도당하 면적은 의미있게 감소하였고 3)인슐린 내성검사상 Kitt가 증가되는 경향을 보였으며 4)수축기, 이완기 혈압과 24시간 단백뇨양이 의미있게 감소하였고 5)체중을 맞추면 내당능 상태가 비슷해졌다가 고농도 포도당 주입시 다시 포도당하 면적이 대조군에서 더 증가되었다. 라미프릴 투여군에서 췌도변형 및 파괴와 탈과립, 췌도내 섬유화가 감소하였다. 결론:본 실험에서는 인슐린 비의존성 당뇨병 동물 모델인 OLETF 쥐에서 당뇨병 발생 이전 단계부터 장기간 라미프릴을 투여할 경우 체중증가가 적었고, 투여군과 대조군의 체중을 맞추었을 때는 내당능상태의 차이가 없아가 고혈당 투여시 다시 투여군과 대조군에서 의미있는 차이를 보였다. 라미프릴 투여가 베타세포의 기능면에서 이로운 효과를 보일 것으로 생각되었다. 따라서 비만한 환자에서 안지오텐신 전환효소 억제제의 장기투여는 단백뇨 감소, 혈압강하 효과 이외에 체중증가의 억제 및 당뇨병 발생을 억제할 가능성을 시사한다. Background : In a Heart Outcomes Prevention Evaluation HOPE study, ramipril, a long-acting angiotensin-converting enzyme (ACE) inhibitor, significantly reduced the death rates the number of myocardial infarctions, strokes, heart failure as well as the risk of complications related to diabetes and of diabetes itself. However, it is known that ACE inhibitors improve glucose tolerance or insulin sensitivity or reduce the incidence of diabetes. Methods : 24 week-old OLETF (Otsuka Long Evans Tokushima Fatty) rats weighing 400 ~ 450 g were used in this study. 4 groups of rats were examined in parallel for 40 weeks. The OLETF rats were randomized for treatment with an aqueous solution of ramipril (5 mg/Kg) daily [OL (RMP), n=10)] and with saline [OL (CON), n=10)]. The LETO rats were also randomized in the same was as the OLETF rats (LT(RMP), n=10, LT (CON), n=10). The blood glucose level, body weight, systolic and diastolic blood pressure was assessed every month. At 3 and 6 months, the 24hrs urinary protein concentration was measured, and as insulin tolerance test and oral glucose tolerance test were conducted in all experimental groups. After 6 months, the body·weight was matched for 2 months in each corresponding group. Subsequently, a 15% sucrose loading was done for 2 months. After the glucose tolerance test, the pancreas was excised and immuno histochemical staining was conducted for insulin to quantify the beta cell mass by a point-counting method. In addition, the islet morphology was evaluated in the pancreas. Results : Ramipril treatment for a period of 6 months improved the 2hr blood glucose level, the area under the glucose curve in the oral glucose tolerance test, insulin sensitivity in addition to lowering significantly systolic and diastolic blood pressure and 24hrs urinary protein level significantly in OLETF rats. Of note, a lower weight gain was observed in both the ramipril-treated animals at 6 months. After weight matching, the AUC g and 2hr blood glucose level values were similar between the corresponding groups, but a 15% sucrose loading worsened the AUC g value. Histologically, the islets were less disorganized and the extent of fibrosis was lower in the ramipril-treated OLETF rats in the trichrome stain. Conclusion : Long-term treatment of ramipril, a long acting angiotensin-converting enzyme inhibitor may be useful for suppressing weight gain and proteinuria in addition to having a protective effect on the islet to harmful stimuli such as hyperglycemia (J Kor Diabetes Asso 25:469~482, 2001).

기어시스템의 횡진동 불안정성에 미치는 설계변수의 영향

노승훈,최면식,정선환 金烏工科大學校 1993 論文集 Vol.14 No.-

Gear system is a fundamental element which is widely used in many mechanical systems. To achieve the high speed and high precision characteristics of the system, which is a current tendency in most mechanical systems, the dynamic stability of a gear system is ver important. In this study, the gear system was simplified into spring supported system to make mathematical model, and the effect of design and operating parameters such as stiffness, length, diameter, and rotating frequency was simulated at various operating conditions. The effect of all these parameters were studied and the method was presented to design stable systems for certain operating conditions.

광중합형과 자가중합형 글라스 아이오노머 시멘트의 변연 접합성에 관한 연구

허승면,조영곤,박일윤 大韓齒科保存學會 1994 Restorative Dentistry & Endodontics Vol.19 No.1

발전기 보호계전 요소분석과 기본 알고리즘 검증에 관한 연구

趙誠振,崔勉松,李承宰 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

In consequence of the fact that the power plant the wattmeter from most as the wattmeter general interpretation focus facility which takes charge of the stable production and a supply of the war potential which is important it minimizes the damage of accident occurrence hour and the hazard which protects a healthy channel high the accuracy and reliability are demanded. In this paper analyzed the operational elements of generator promotion of virtue conclusion protection ratio differential relay (87G), Earth current differential relay (87GN), Revers power relay (32),Synchronization relay (25), automatic Synchronization relay (25A), frequency relay (81) from the dissertation which it sees and the maul trap to grasp a basic principle it led and a basic algorithm it verified. This paper is dissertation which it sees leads and there is a place where it develops the basic algorithm which it follows in protection element analysis and operational for the domestic technical development of the generator protection relay field which depends in advanced foreign nation production company quality.

Ⅱ급 와동에서 각종 구치용 수복물의 파절강도에 관한 실험적 연구

조영곤,허승면,이계혁 大韓齒科保存學會 1993 Restorative Dentistry & Endodontics Vol.18 No.2

The purpose of this study was to evaluate the fracture strength of class Ⅱ restored premolars with amalgam, posterior composite, amalgam-Ketac silver, resin-Ketac silver restorations at marginal ridge. Fifty extacted maxillary and mandibular premolar teeth that were caries free, fracture free, and restoration free were selected and randomly divided into five groups : Group 1:10 intact teeth, Group 2:10 teeth with class Ⅱ cavities and restored with, amalgam, Group 3:10 teeth with class Ⅱ cavities and restored with posterior resin, Group 4:10 teeth with class Ⅱ cavities and restored with amalgam-ketac silver, Group 5:10 teeth with class Ⅱ cavities and restored with resin-Katac silver. All teeth were mounted in base of dental stone within metal rings of 2cm diameter, exposing only the crown portion. Class Ⅱ mesio-occlusal or disto-occlusal cavities were prepared into specimens of Group 2 throung 5 by using a No. 710 fissure bur. The occlusal portion was prepared to a faciolingual width of 1.5mm and a pulpal depth of 1.5mm. The proximal protion was prepared to a faciolingual width of 4mm, a occlusogingival height of 4mm, and a gingival floor of 1.5mm. The teeth in Group 2 and 3 were resotored with silver amalgam and posterior resin respectively. In Group 4 and 5, proximal portions were first filled with Ketac silver 1.5mm gingivally and remaining cavities were restored with amalgam and posterior resin respectively. All specimens were stored in 100% relative humidity at 37℃ for 48 hours before testing. All teeth were subjected to a compressive load in a Universal Instron Testing Machine at marginal ridges. The loads required to fracture the restorations were recorded in killograms and the data obtained were subjected to statisticall analysis. The results were all follow: 1. The fracture strength of Group 1 which were unprepared were 100±10.0 kg and the higher values than Group 2, 3, 4, 5 which were prepared and resotred. 2. In restored groups, Group 2 had the higher fracture strength(81.8±12.4 kg) than other groups and Group 4 had the lowest fracture strength(66.8±9.2kg). 3. There were significant differences between fracture strength of between Group 1 and Group 3, 4, 5(P<0.05), but not significant difference between fracture strength of Group 2, 3, 4, 5(P>0.05).

송전계통 보호계전기 동작 시뮬레이터

민병운,최면송,이승재 明知大學校 産業技術硏究所 1999 産業技術硏究所論文集 Vol.18 No.-

When faults occur in power systems, the protective relay plays a roles of protecting power systems according to its setting values. There it is important that the protective relay have high selectiveity and sencitivity performance in power system by correct setting the relays. This paper proposed a method to testify the reliability of digital relay as simulating the response of relay at the faults in the real power system operation environment.

광중합형 글래스 아이오노머 시멘트의 치질접착에 관한 연구

문주훈,조영곤,허승면 大韓齒科保存學會 1996 Restorative Dentistry & Endodontics Vol.21 No.2

항진균제 알릴아민류의 합성과 생물학적 평가

정병호,조원제,천승훈,박면지,유진철,천문우 朝鮮大學校 1998 藥學硏究誌 Vol.19 No.2

For the development of antifungal agents. modification of naftifine which exhibits significant antimycotic activity was performed by replacing the naphthalene ring of it to hetero cyclic rings such as morpholine. benzothiazole. piperidine and pyridine derivatives. The synthesized compounds were tested in vitro antifungal activity against five different fungi with naftifine as a comparative antimycotic molecule. From the biological evaluation two compounds. (E)-N-(3-phenyl-2-propenyl)-N-(4-piperidinylmethyl)amine(3d) and (E)-N-(3-phenyl-2-propenyl)-N-(3-pyndylmethyl)amine(3D) showed relatively noticeable activity(MIC=50㎍/㎖). On the other hand. the other compounds had no activity.