흰쥐에서 반복적 스트레스가 GABA성 신경전달에 미치는 영향

김창수,이종범,성형모,시현석,김진성,박형배,정성덕,하정희 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

연구목적 : 일반적으로 생체는 각종 스트레스에 반응하여 적응(adaptation) 기전이 나타난다. 이러한 적응 기전과 관련하여 포유동물의 중요한 억제성 신경전달계인 GABA성 신경전달계의 스트레스성 자극에 대한 반응양상에 대한 연구가 진행되고 있다. 신경전달의 변화양상은 부하된 스트레스의 종류 및 기간에 따라 다양하게 나타나므로 더욱 상세한 연구를 필요로 한다. 방 법 : 본 연구에서는 하루 2시간의 부동화 스트레스를 반복적으로 2주간 부하한 흰쥐의 뇌조직에서 GABA성 신경전달의 변화 양상을 관찰하고자 하였다. 수용체 결합반응을 통하여 GABA 수용체의 각 component별 배위자들의 각 수용체에 대한 결합 반응의 변화 양상을 정상 대조군와 비교하여 보고, 유의한 변화가 있는 부분의 상세한 검정을 위하여 포화결합반응을 통하여 친화력(Kd) 및 최대결합력(Bmax)에서의 변화양상을 검색하였다. 뿐만 아니라 GABA성 신경전달에 관여하는 중요한 신경전달체인 내인성 GABA의 함량과 대사효소인 GABA transaminase의 활성도의 변화양상도 검색하였다. 결 과 : 연구결과 반복적인 부동화 스트레스는 GABA 수용체 효현제인 [³H]muscimol의 흰쥐 뇌조직 GABA 수용체에 대한 결합도에는 별다른 영향을 미치지 않았다. 대뇌피질조직의 GABA 대사효소인 GABA transaminase 활성도를 감소시켰으나, 피질조직외의 다른 조직의 GABA transaminase 활성도나 내인성 GABA의 농도에는 별다른 영향을 미치지 않았다. 반복적인 부동화 스트레스를 부하한 흰쥐의 대뇌피질, 해마 및 시상하부 조직에서는 [³H]flunitrazepam의 수용체 결합도가 감소하였으며, 포화결합 반응결과 수용체의 수가 감소하였음을 확인하였다. 결 론 : 이상의 결과를 요약하면, 흰쥐에게 부하한 반복적인 부동화 스트레스는 뇌조직의 benzodiazepine 수용체의 수를 감소시킴으로써 GABA성 신경전달을 감소시키며, 이러한 GABA성 신경전달계의 변환은 생체에서 스트레스로 인한 행동장애의 한 기전이 될 수 있을 것으로 생각되었다. Objectives : Changes of GABAergic neurotransmission in response to the application of different types of environmental stress have been the subject of research for over two decades. However, the nature of the changes induced by stress appear to show a dependent phenomena on the type and duration of stressor agent employed. Methods : For this reason, this study was performed to observe the effects of repeated stress on the radioligands binding to GABAA/benzodiazepine receptors of discrete brain regions. The author also examined the activity of GABA transaminase and the concentration of endogenous GABA. Male Sprague-Dawley rats, weighing 150-200g were forced to suffer an immobilization stress for 2 hours during 14 consecutive days. Results : Repeated immobilization stress decreased the binding of [3H]flunitrazepam on the benzodiazepine receptor in the cortex, hippocampus and hypothalamus. Saturation experiments followed by scatchard analyses of the results showed decreased density of benzodiazepine receptor and the affinity remained unchanged. Repeated immobilization stress did not affect the binding of [3H]muscimol on the GABAA receptor, the activity of GABA transaminase, and the concentration of endogenous GABA in the brain regions. Conclusions : From these results, it can be concluded that repeated immobilization stress modulated GABAergic neurotransmission via downregulation of the benzodiazepine receptor in the brain.

수정 아인쉬타인 방법에 의한 만경강 유입 유사량 산정

김형산, 맹승진, 정세웅, 이승욱 忠北大學校 農業科學硏究所 2011 農業科學硏究 Vol.27 No.4

-본문참고-

전방경추부 응합술시 단피질 나사못과 양피질 나사못의 비교연구

박형천,윤승환,박현선,김은영 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.5

베타-아밀로이드 펩타이드로 유발된 신경세포사와 tau 인산화와의 연관성

김지형,홍혜남,이종환,황승준,박형섭,이흥식,김동호 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.4

연령에 따른 한국인의 음향지표 변화와 특성

김형태,조승호,윤성문,선동일,김민식 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.1

급성 B형 간염의 회복 후 말초혈액 단핵구에서의 HBV DNA검출

명승재,이영상,김형건,이근찬,박능화,정영화,서동진 大韓消化器病學會 1997 大韓消化器病學會誌 Vol.29 No.1

사립체 DNA 뉴클레오타이드 3243 A→G 돌연변이 [MTTL1^*MELAS3243G]에 의한 MELAS 증후군

박기형,김승현,이영주,김희태,김주한,김명호,박성섭 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

치아 우식증의 조기 진단을 위한 QLF-D 개발

박형주,김종수,유승훈,신지선 大韓小兒齒科學會 2011 大韓小兒齒科學會誌 Vol.38 No.4

QLF-D system composed with DSLR(digital single-lens reflex) camera, and the images of natural enamel caries and artificial caries was developed from 2 days to 14 days captured by QLF-D system. The correlation between lesion depth of the polarized microscope and luminosity ratio of QLF-D image was analyzed and the results were summarized as follows: 1. The Pearson correlation value between the lesion depth of polarized microscope images and luminosity ratio of QLF-D images was 0.969(p<0.01). 2. From Regression analysis of lesion depth from polarized image by demineralized period, the equation was y = 8.67x - 1.16(p<0.05). 3. From Regression analysis of luminosity ratio from QLF-D image by demineralized period, the equation was y = 3.53x + 6.42(p<0.05). From the results, QLF-D system can detect the enamel caries at the very early stage and can monitor the progression of demineralization and remineralization. For the convenient use of QLF-D system in the laboratory, the image analysing software was needed to analyze of interest site of enamel caries lesion. 저자는 DSLR(digital single-lens reflex) 카메라를 이용한 QLF-D 시스템을 고안하여, 사람의 유구치에 자연 발생된 법 랑질 초기 우식증을 관찰하고, 유구치 법랑질 시편을 대상으로 2일부터 14일까지 인공 탈회시킨 후 탈회 기간에 따른 상관관 계를 편광 현미경 소견과 비교 분석한 결과 다음과 같은 결과를 얻었다. 1. 탈회 기간에 따른 편광 현미경 상의 병소 깊이와 QLF-D 영상의 광밀도 값 비율 간의 피어슨 상관 계수는 0.969였다 (p<0.01). 2. 편광현미경에서 측정된 병소 깊이의 탈회 기간에 대한 회귀분석 결과 y=8.67x-1.16의 회귀 방정식이 산출되었다 (p<0.05). 3. QLF-D 영상에서 측정된 광도값 비율의 탈회 기간에 대한 회귀분석 결과 y=3.53x+6.42의 회귀 방정식이 산출되었다 (p<0.05).

해안 잠재 수변공간 개발 대상지 선정에 관한 연구 : 기장군을 중심으로

민병형,김가현,유상호,민상철,이승휘 東亞大學校 海洋資源硏究所 2002 硏究論文集 Vol.14 No.1

This study is determin digging and development preferential ranking which is the potential area of seafront in Busan Kijanggun according to rise citizen of literary life and increase desire of culture life. Obtained results of this study are summarized as follows. 1. Decide to development preferential program that eight area selected from the potential area of seafront to position in Busan Kijjanggun. 2. Appraisal standard I decided to development preferential program which is Yunhwa gigu, Yonggung temple, Juksung estuary, Sirangdae, Munjung· Mundong harbor, Seoam harbor, Hoyamlee and Sinpyung harbor. 3. Appraisal standardII decided to development preferential program which Yunhwa gigu, Yonggung temple, Juksung estuary, Hoyamlee and Sirangdae, Seoam harbor, Sinpyung harbor and Munjung ·Mundong harbor get the same grade. 4. As a result of comparative appraisal standard I, II is that first~third program is equal but it was changed from fourth program. 5. When potential area in seafront appraised by Lee unit, appraisal standard I is superior than appraisal standardII.

이화명충에 對한 "통일벼"의 耐蟲性 機作에 關한 硏究

李炯來,崔承允 서울大學校 農科大學 1977 서울대농학연구지 Vol.2 No.1

This study was conducted to evaluate the nature of the variety Tongil (Suweon 213-1) in resistance to the striped rice borer, Chilo suppressalis Walker, comparing with those of Rexoro (susceptible check) and TKM-6 (resistant check) selected at IRRI. 1. The striped rice borer moths much more preferred the variety Tongil for ovipositon than the Rexoro and TKM-6. The variety Tongil had more egg masses and more number of eggs than the varieties Rexoro and TKM-6, while the variety TKM-6 having more egg masses and more number of eggs than the variety Rexoro. This reaction was consistent throughout the test regardless of the number of tillers per hill. 2. In laboratory, preference of larvae for feeding was studied with 5cm of stem pieces of the varieties. The result showed, in contrast to the case of ovipositional preference, that the striped rice borer larvae least preferred the stems of the variety Tongil among the test varieties, while larvae much more preferred the stems of Rexoro than those of TKM-6. 3. The larval weights at 20 days later infested on the 40 day-old plants were the lowest on the variety Tongil among the test varieties. On the variety Rexoro the larvae had heavier body weights (43.0mg), higher pupation (64.9%) and higher adult emergence (83.3%) than those on the variety Tongil (larval weight 30.3mg, pupation 60. 9%, adult emergence 60.7%) and the variety TKM-6 (larval weight 35.7mg, pupation 56.3%, adult emergence 51.9%). The pupal weights, however, were not consistent among the test varieties and/or sexes in comparison with the larval weight, pupation and adult emergence above mentioned. 4. Field experiments ildicated that the incidence of dead hearts at 70 days after transplanting was relatively higher on the variety Tongil (11.1%) than those on the varieties Rexoro (8.9%) and TKM-6 (8.4%), and the incidence of white heads at harvest was in contrast to the dead hearts lower on Tongil (9.8%) than those on Rexoro (27.4%) and TKM-6(13.9%). At harvest lower larval observed on Tongil (49 larvae/40 hills) than those on the susceptible variety Rexoro (104 larvae/40 hills) and on the resistant variety TKM-6 (70 larvae/40 hills). The average larval weights collected from three test varieties at harvest were 80.5mg from Tongil, 83.7mg from TKM-6 and 99.6 mg from Rexoro. 5. Increased nitrogen fertilizer application to the variety Tongil, the striped rice borer damage was increased. Also, preferecne of larvae for feeding significantly increased with the increase of nitrogen fertilizer application. 6. Any specific association between the plant characters and striped rice borer resistance could not be found. The variety Tongil even having large number of tillers, short plant height, large stem, broad leaf, etc., had still high preference of months for oviposition, low preference of larvae for feeding, low damage, and relatively high antibiosis. 7. Resistance of the variety Tongil to the striped rice borers seemed to be associated with the low feeding preference and the relatively high antibiosis, not associated with the ovipositional preference.