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Seulgi Mun,Mi Young Noh,Yasuyuki Arakane 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
Cuticular proteins (CPs) and the polysaccharide chitin are the major components of the exo- and endocuticular layers or procuticle. CPs contain a conserved sequence known as the Rebers & Riddiford (R&R) motif, which may function as a chitin-binding domain that helps to coordinate the interaction between chitin fibers and the protein network. We identified two highly abundant RR-2 CPs, TcCPR18 and TcCPR27, in protein samples extracted from elytra (rigid cuticle) of Tribolium castaneum adults and determined that these two CPs are required for rigid cuticle morphology. In this study, we identified the third most abundant protein (TcCP30) extracted from the elytra, and cloned a full-length cDNA. It encodes a very unusual 171 amino acid residue protein of which 36% of the residues of the mature protein are Glu, 21% are His, 19% are Arg, and 16% are Gly, organized in a regular pattern but not R&R consensus motif. TcCPR18 and TcCPR27 genes are expressed at 4 d-old pupae, while TcCP30 is highly expressed at 5 d-old pupae (last pupal stage) and 0 d-old adults. Immunohistochemical studies revealed the presence of TcCP30 in rigid adult cuticle (e.g. elytron, pronotum and ventral abdomen) but not soft cuticle (e.g. hindwing and dorsal abdomen). Injection of dsRNA for TcCP30 into late instar larvae had no affect on larval and pupal growth and development. The subsequent pupal-adult molt, however, more than 50% adults were unable to shed their exuvium and died. In addition, the resulting adults exhibited wrinkled, warped and split elytra. TcCP30-deficient adults could not fold their hindwings properly. These results indicate that TcCP30 may play critical roles in rigid adult cuticle formation, development and insect growth and survival. This work was supported by NRF (NRF-2012R1A2A1A01006467).
Effect of changes in head postures during use of laptops on muscle activity of the neck and trunk
( Seulgi Lee ),( Yeseb Lee ),( Yijung Chung ) 물리치료재활과학회 2017 Physical therapy rehabilitation science Vol.6 No.1
Objective: This study tried to examine the muscle activity of the neck and trunk according to head posture changes during use of laptops. Design: Cross-sectional study. Methods: This study included nineteen young men and women. All subjects maintained each posture while practice typing on laptops for ten minutes with a 5-minute break between each posture. For the neutral head posture, the subjects practiced typing while pulling their chins down towards the Adam`s apple and were able to look at their knees while having their external auditory meatus, acromion, and greater trochanter vertical to the ground. For the natural head posture, they practiced typing while balancing their posture between extension and flexion of the cervical vertebrae by themselves without any guidelines. While a forward head posture was created by having their heads face the front horizontal to the rope hanging from the ceiling, they practiced typing with their external auditory meatus located in the place which was 5 cm in front of the vertical plane. The subjects used general word process but practiced typing with accuracy and high speed. Muscle activities were randomly measured using surface electromyography according to each postures. Results: The research result had found that muscle activity with the natural head posture was more significantly reduced than that of the forward head posture in the sternocleidomastoid muscle, upper trapezius, cervical vertebral spinae, and thoracic vertebrae spinae muscles (p<0.05) and that the neutral head posture was more significantly reduced than that of forward head posture in the upper trapezius, cervical vertebral spinae, and thoracic vertebrae spinae muscle activity (p<0.05) with significant increases in lumbar spinae muscle activity (p<0.05). Also, muscle activity with the neutral head posture significantly increased more in the sternocleidomastoid muscle and lumbar spinae than that of the natural posture (p<0.05). Conclusions: Our study results suggest that in order to prevent musculoskeletal pain, the neutral head posture with use of laptops is effective in reducing load to the shoulders and vitalizing the postural muscles.
Adipogenic function of tetranectin mediated by enhancing mitotic clonal expansion via ERK signaling
( Seulgi Go ),( Jihyun Park ),( Safikur Rahman ),( Juno Jin ),( Inho Choi ),( Jihoe Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2021 BMB Reports Vol.54 No.7
Tetranectin (TN), an adipogenic serum protein, enhances adipocyte differentiation, however, its functional mechanism has yet to be elucidated. In the present study, we investigated the adipogenic function of TN by using medium containing TN-depleted fetal bovine serum (TN-del-FBS) and recombinant mouse TN (mTN). The adipocyte differentiation of 3T3-L1 cells was significantly enhanced by mTN supplementation essentially at differentiation induction, which indicated a potential role of the protein in the early differentiation phase. The adipogenic effect of mTN was more significant with insulin in the differentiation induction cocktail, implicating their close functional relationship. mTN enhanced not only the proliferation of growing cells, but also mitotic clonal expansion (MCE) that is a prerequisite for adipocyte differentiation in the early phase. Consistently, mTN increased the phosphorylation of ERK in the early phase of adipocyte differentiation. Results of this study demonstrate that the adipogenic function of mTN is mediated by enhancing MCE via ERK signaling. [BMB Reports 2021; 54(7): 374-379]
Seulgi Mun,Mi Young Noh,Yasuyuki Arakane 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04
Insect cuticle/exoskeleton covering the entire external surface of the body is essential for protecting insects from various environmental stresses. Tyrosine metabolism plays a major role in not only the darkening of cuticle but also its hardening. In this work, we have focused on the functional analysis of nine genes involved in tyrosine-mediated cuticle tanning (pigmentation and sclerotization) pathway in Tenebrio molitor, which has a unique adult cuticle coloration, dark/black dorsal thorax and elytron, and reddish ventral thorax and abdomen. The temporal and spatial expression patterns of the genes were analyzed by real-time PCR, and RNA interference (RNAi) was performed to study the functional importance of these genes in cuticle coloration and/or hardening in T. molitor. This work was supported by NRFs (NRF-2015R1A2A2A01006614 and NRF-2015R1A6A3A04060323).
Seulgi Lee,고은주,채희주,Chieko Minami 한국마케팅과학회 2017 마케팅과학연구 Vol.27 No.2
Countries’ specifi c traditional cultures are becoming popular subjects for commercialization. The research discussed here focuses on “traditional cultural products” (TCPs) and investigates consumer attitudes and behaviors with respect to cultural aspects. Consumer attitudes and the purposes of consumption within the commercialization of traditional cultures encircling cultural authenticity are scrutinized. Results show that while the personal factor of consumers’ open-mindedness toward other cultures positively infl uences their attitudes toward TCPs, the ethnocentrism and familiarity of the culture have negative eff ects. This analysis of the purchasing purpose model of culture products among three countries – Korea, China, and Japan – shows that positive attitudes toward the aesthetic elements are crucial in raising the will to consume traditional cultural goods. Moreover, Korean and Chinese consumers have signifi cantly considered the cultural/historical authenticity of goods whereas Japanese consumers have found craftsmanship to be more vital.
The effect of lower extremity postures on trunk kinematics while sitting
Seulgi Kim,Ilseok Lee,Sangeun Jin 대한인간공학회 2018 대한인간공학회 학술대회논문집 Vol.2018 No.5
Objective: This study aimed to investigate the effect of lower extremity posture on lumbar, thoracic, cervical and head postures while sitting. Background: Previous studies have largely contributed to an understanding of the sitting biomechanics, but those studies have considered the low back as an isolated system and mislead the functional interaction between lower extremity and trunk. Method: Seventeen healthy participants were recruited from the university population, and were asked to sit on a chair with six different lower extremity postures (2 trunk-thigh angles (90° and 135°) × 3 knee angles (45°, 90° and 180°)) (5 replication). Each sitting trials continued for 10 seconds without any request or restriction on the trunk posture. The body kinematics was measured with a motion capture system including the lumbar flexion angle, the pelvic flexion angle, the head flexion angle, and the rounded shoulder angle. Results: Significant effects of the trunk-thigh angle and the knee angle on all dependent measures were observed. First, the bigger trunk-thigh angle (135°) showed better lumbar lordosis, smaller head flexion angle, and less rounded shoulder, suggesting the bigger the trunk-thigh angle, the better the whole trunk postures. Second, the bigger knee flexion angle (180°) negatively influenced on all trunk posture measures, suggesting that the extended knee joint tightens hamstring muscles and pulls the pelvis backward, and finally results in the bad trunk posture such as less lordotic lumbar and thoracic postures, more head flexion angle and more rounded shoulder angle. Conclusion: All dependent variables capturing trunk kinematics clearly denoted the significant influence of the lower extremity posture on the low back, upper back, and head postures. Application: The office chair should be designed under the consideration of the lower extremity postures such as the position of foots, the knee supporter, and the seat inclination angle.