Maintaining canine sperm function and osmolyte content with multistep freezing protocol and different cryoprotective agents

Setyawan, E.M.N.,Kim, M.J.,Oh, H.J.,Kim, G.A.,Jo, Y.K.,Lee, S.H.,Choi, Y.B.,Lee, B.C. Academic Press 2015 Cryobiology Vol.71 No.2

Cryopreservation procedures cause osmotic stress to spermatozoa following cryoinjury and reduce their content of osmolytes. Conventional method for cryoprotectant loading and dilution on canine semen freezing which could be categorized in single step protocol, makes decreasing in sperm performance such as motility, morphology and viability. Therefore, the objective of the present study was to determine if a multistep protocol using glycerol or ethylene glycol can be used to overcome the osmotic sensitivity of canine spermatozoa, and to identify osmolytes that were involved in regulation of osmotic stress. A multistep protocol, comprising serial loading and dilution of cryoprotective agents by dividing the total volume of extender into 4 steps (14%, 19%, 27%, and 40%) every 30s, was compared to a single step method. Frozen-thawed spermatozoa in the multistep group showed superior quality (P<0.05) compared with the single step process in progressive motility (23.3+/-1.3% vs. 12.5+/-1.6%), intact membranes (66.5+/-2.8% vs. 49.5+/-2.6%) and bent tail (29.2+/-3.2% vs. 46.2+/-1.9%). Multistep also succeeded in minimizing loss of the osmolytes carnitine (20.6+/-2.0nmol/U G6PDH vs. 10.8+/-2.1nmol/U G6PDH) and glutamate (18.4+/-1.6nmol/U G6PDH vs. 14.4+/-0.8nmol/U G6PDH) compared to the single step group. Moreover, glycerol with multistep was more advantageous for maintaining sperm quality than ethylene glycol. In conclusion, the multistep protocol with glycerol can be used to improve the morphology, motility and osmolytes content of frozen-thawed canine spermatozoa.

Synthesis and Characterization of Graphene Oxide from High Rank Coal

Setyawan Daru,Shaur Ahmad,Yoo Jiho(유지호),Kim Sang Do(김상도),Lim Jeonghwan(임정환),Rhim Young Joon(임영준),Choi Ho Kyung(최호경),Lee Sihyun(이시훈),Chun Dong Hyuk(전동혁) 한국에너지기후변화학회 2016 한국에너지기후변화학회 학술대회 Vol.2016 No.5

The Effect of Temperature and Residence Time of Torrefaction on the Torrefied Biomass Properties

Setyawan Daru,Yoo Jiho,Kim Sang Do,Lim Jeonghwan,Rhim Young Joon,Choi Ho Kyung,Lee Sihyun,Chun Dong Hyuk 한국에너지기후변화학회 2016 한국에너지기후변화학회 학술대회 Vol.2016 No.11

Spermine reduces reactive oxygen species levels and decreases cryocapacitation in canine sperm cryopreservation

Setyawan, Erif Maha Nugraha,Kim, Min Jung,Oh, Hyun Ju,Kim, Geon A.,Jo, Young Kwang,Lee, Seok Hee,Choi, Yoo Bin,Lee, Byeong Chun Elsevier 2016 Biochemical and biophysical research communication Vol.479 No.4

<P><B>Abstract</B></P> <P>The objective of this study was to determine the ability of spermine to act as an antioxidant in scavenging reactive oxygen species (ROS), maintaining sperm function and decreasing cryocapacitation after cryopreservation. Although motility did not increase with spermine treatment, values for membrane integrity were significantly increased (P < 0.05). Higher percentages of linearity and straightness with a lower amplitude of lateral head displacement (ALH) indicated that spermine inhibits hyperactivation. Concentrations of intracellular and extracellular ROS were decreased in the treatment group (P < 0.05). Higher expression of an anti-apoptotic gene (<I>Bcl</I>-2) and lower expression of a pro-apoptotic gene (<I>Bax</I>), together with decreased expression of the mitochondrial ROS modulator <I>ROMO</I>1, DNA repair due to oxidative damage (<I>OGG</I>1), spermine synthase (<I>SMS</I>), NADPH oxidase associated with motility (<I>NOX</I>5) and spermine amino oxidase (<I>SMOX</I>), all showed that 5.0 mM spermine treatment was beneficial to spermatozoa. Furthermore, the proportion of live spermatozoa with intact acrosomes after thawing in the treatment group was higher than in the control. After incubation in canine capacitating medium, numbers of live capacitated spermatozoa with reacted acrosomes were higher than in the control. Our results indicate that 5.0 mM spermine is an optimal concentration for maintaining sperm function, reducing ROS production, preventing apoptosis and adverse effects of cryocapacitation during canine sperm cryopreservation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Spermine improved kinematic parameters and membrane integrity of frozen sperm. </LI> <LI> Spermine decreased intracellular and extracellular ROS during cryopreservation. </LI> <LI> Nitro Blue Tetrazolium followed by ELISA was used for measuring ROS level. </LI> <LI> Spermine reduced cryocapacitation during canine sperm cryopreservation. </LI> <LI> Spermine increased the number of capacitated sperm after CCM incubation. </LI> </UL> </P>

Development of hybrid loop heat pipe using pump assistance for cooling application on high heat flux device

Iwan Setyawan,Nandy Putra,Imansyah Ibnu Hakim,Ridho Irwansyah 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.8

Loop heat pipe (LHP) is an effective two-phase heat transfer device that utilizes evaporation and condensation of a working fluid. LHP is widely used in a passive cooling system, highly reliable and a very high thermal conductive device. However, extreme power density can cause dry-out in the evaporator section of LHP and might hinder the performance of the cooling system. The purpose of this research is to design and manufacture a new prototype of LHP by modifying the conventional LHP. The modification was performed by adding a diaphragm pump, called hybrid loop heat pipe (HLHP). The experimental results show that the installation of pumps in the modified LHP was able to prevent the occurrence of a dry out and reduce the operating temperature. Under a constant conductance mode, the pump was activated for 20 minutes and succeded in forcing the system to a new stable condition with a significant temperature drop. These results indicate that HLHP is very promising as a two-phase cooling system that can be proposed for devices that produce high heat flux.

Despite the donor's age, human adipose-derived stem cells enhance the maturation and development rates of porcine oocytes in a co-culture system

Nugraha Setyawan, Erif Maha,Oh, Hyun Ju,Kim, Min Jung,Kim, Geon A.,Lee, Seok Hee,Choi, Yoo Bin,Ra, Kihae,Lee, Byeong Chun Elsevier 2018 Theriogenology Vol.115 No.-

<P><B>Abstract</B></P> <P>The paracrine interactions between cumulus-oocyte complexes (COCs) and follicular somatic cells during <I>in vitro</I> maturation (IVM) were investigated. To optimize IVM conditions, many studies have applied exogenous growth factors and cell feeding/co-culture systems using various cell types to replicate the natural follicular microenvironment during IVM. A potential candidate as cell feeders is adipose-derived stem cells (ASCs) which secrete high levels of growth factors that have roles in oocyte maturation. However, the cell donor's age should be considered because biological aging also occurs in stem cells. In the present study, the contributions of ASCs from young and old donors on an IVM co-culture system were analyzed by comparing the oocyte maturation rate, cumulus expansion index, preimplantation development after parthenogenetic activation (PA), and expression of growth factor signaling genes related to oocyte maturation in ASCs, oocytes and cumulus cells under the same culture conditions. Our study demonstrated that the confluence, viability and cell size of ASCs between young and old donors were not significantly different and only the Fibroblast Growth Factor 2 (<I>FGF2</I>) signaling gene showed higher expression in ASCs from young donors. The oocyte maturation rate in the young donor group (87.8 ± 1.2%) was significantly higher than in the old donor (81.1 ± 2.1%) and control (73.8 ± 2.1%) groups. After IVM, most gene expression levels in oocytes and cumulus cells in the co-culture groups were higher than in the control but the apoptotic ratios were reduced. The blastocyst development rates were not different between the young and old donor groups (23.9 ± 1.3% and 20.7 ± 0.8%, respectively) but the percentages were higher in both groups compared to the control group (16.4 ± 1.2%). A similar pattern was also found for blastocyst total cell numbers in that the young donor group (87.5 ± 5.2 cells) was not different than the old donor group (77.5 ± 3.4 cells) but both groups exhibited higher number of cells compared with the control group (57.9 ± 6.0 cells, p < .05). Our study strongly suggested that the co-culture IVM system with ASCs greatly improved the maturation and development rates of porcine oocytes. Moreover, ASCs from young donors more effectively supported porcine oocyte maturation than those from old donors although this difference did not translate into improved developmental competence.</P> <P><B>Highlights</B></P> <P> <UL> <LI> IVM with co-culture strategy improved the effectivity of oocyte maturation. </LI> <LI> The confluence, viability and cell size between young and old donor were similar. </LI> <LI> The FGF2 signalling gene showed higher expression in young ASCs donor. </LI> <LI> Maturation rate in young donor was the highest than old donor and control groups. </LI> <LI> Co-culture IVM system up regulated most of signalling genes in oocytes and cumulus. </LI> </UL> </P>

Torrefaction Effect on the Grindability Properties of Several Torrefied Biomasses

( Daru Setyawan ),( Jiho Yoo ),( Sangdo Kim ),( Hokyung Choi ),( Youngjoon Rhim ),( Jeonghwan Lim ),( Sihyun Lee ),( Dong Hyuk Chun ) 한국화학공학회 2018 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.56 No.4

Torrefaction is the promising process of pretreating biomass materials to increase the quality of their energy, especially to upgrade the materials’ grindability so that it is suitable for a commercial pulverizer machine. In this study, torrefaction of oak, bamboo, oil palm trunk, and rice husk was carried out under different torrefaction temperatures (300 ℃, 330 ℃, and 350 ℃) and different torrefaction residence times (30, 45, and 60 minutes). Complete characterization of the torrefied biomass, including proximate analysis, calorific value, thermogravimetric analysis, mass yield, energy yield, and grindability properties (Hardgrove Grindability Index) was carried out. Increasing the torrefaction temperature and residence time significantly improved the calorific value, energy density (by reducing the product mass), and grindability of the product. Furthermore, for commercial purposes, the torrefaction conditions that produced the desired grindability properties of the torrefied product were 330 ℃-30 minutes and 300 ℃-45 minutes, and the latter condition produced a higher energy yield for bamboo, oil palm trunk, and rice husk; however, torrefaction of oak did not achieve the targeted grindability property values.

Soil Surface Assessment under Plantation Forest in South Sumatra using Landscape Function Analysis Procedure

Dwi Setyawan 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Solving the Team Orienteering Problem with Particle Swarm Optimization

The Jin Ai,Jeffry Setyawan Pribadi,Vincensius Ariyono 대한산업공학회 2013 Industrial Engineeering & Management Systems Vol.12 No.3

The team orienteering problem (TOP) or the multiple tour maximum collection problem can be considered as a generic model that can be applied to a number of challenging applications in logistics, tourism, and other fields. This problem is generally defined as the problem of determining P paths, in which the traveling time of each path is limited by Tmax that maximizes the total collected score. In the TOP, a set of N vertices i is given, each with a score Si. The starting point (vertex 1) and the end point (vertex N) of all paths are fixed. The time tij needed to travel from vertex i to j is known for all vertices. Some exact and heuristics approaches had been proposed in the past for solving the TOP. This paper proposes a new solution methodology for solving the TOP using the particle swarm optimization, especially by proposing a solution representation and its decoding method. The performance of the proposed algorithm is then evaluated using several benchmark datasets for the TOP. The computational results show that the proposed algorithm using specific settings is capable of finding good solution for the corresponding TOP instance.

Clinical assessment after human adipose stem cell transplantation into dogs

이석희,Erif M.N. Setyawan,Yoo-Bin Choi,Jeong Chan Ra,Sung Keun Kang,Byeong Chun Lee,김지은 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.3

Adipose tissue-derived stem cell (ASCs) are an attractive source of stem cells with therapeutic applicability in various fields for regenerating damaged tissues because of their stemness characteristics. However, little has reported on evaluating adverse responses caused by human ASC therapy. Therefore, in the present study, a clinical assessment after human ASC transplantation into dogs was undertaken. A total of 12 healthy male dogs were selected and divided into four groups: saline infusion, saline bolus, ASC infusion, and ASC bolus groups. Physical assessment and blood analysis were performed following ASC transplantation, and the concentrations of angiogenic factors, and pro- and anti-inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA). There were no adverse vital sign responses among the dogs. Blood analyses revealed no remarkable complete blood count or serum chemistry results. ELISA results for angiogenic and anti-inflammatory factors including matrix metalloproteinase 9 (MMP9), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and interleukin-10 (IL-10) were significantly higher in the two ASCs groups than in the controls. In conclusion, this study demonstrated that transplantation of human ASCs produced no adverse effects and could be used safely in dogs. In addition, human ASCs could be involved in modulating secretions of angiogenic factors including MMP9, VEGF, bFGF, and HGF and anti-inflammatory factor IL-10.