Synbiotics use for preventing sepsis and necrotizing enterocolitis in very low birth weight neonates: a randomized controlled trial

Pehlevan, Ozge Serce,Benzer, Derya,Gursoy, Tugba,Karatekin, Guner,Ovali, Fahri The Korean Pediatric Society 2020 Clinical and Experimental Pediatrics (CEP) Vol.63 No.6

Background: Probiotics and prebiotics have strain-specific effects on the host. Synbiotics, a mixture of probiotics and prebiotics, are proposed to have more beneficial effects on the host than either agent has alone. Purpose: We performed a randomized controlled trial to investigate the effect of Lactobacillus and Bifidobacterium together with oligosaccharides and lactoferrin on the development of necrotizing enterocolitis (NEC) or sepsis in very low birth weight neonates. Methods: Neonates with a gestational age ≤32 weeks and birth weight ≤1,500 g were enrolled. The study group received a combination of synbiotics and lactoferrin, whereas the control group received 1 mL of distilled water as placebo starting with the first feed until discharge. The outcome measures were the incidence of NEC stage ≥2 or late-onset cultureproven sepsis and NEC stage ≥2 or death. Results: Mean birth weight and gestational age of the study (n=104) and the control (n=104) groups were 1,197±235 g vs. 1,151±269 g and 29±1.9 vs. 28±2.2 weeks, respectively (P>0.05). Neither the incidence of NEC stage ≥2 or death, nor the incidence of NEC stage ≥2 or late-onset culture-proven sepsis differed between the study and control groups (5.8% vs. 5.9%, P=1; 26% vs. 21.2%, P=0.51). The only significant difference was the incidence of all stages of NEC (1.9% vs. 10.6%, P=0.019). Conclusion: The combination of synbiotics and lactoferrin did not reduce NEC severity, sepsis, or mortality.

Lysophosphatidylethanolamine (LPE) Improves Fruit Size, Color, Quality and Phytochemical Contents of Sweet Cherry c.v. '0900 Ziraat'

Ozgen, Mustafa,Serce, Sedat,Akca, Yasar,Hong, Ji Heun Korean Society of Horticultural Science 2015 원예과학기술지 Vol.33 No.2

Lysophosphatidylethanolamine (LPE) affects the quality of flowers, fruits, and other horticultural products. Studies have provided evidence that LPE can accelerate ripening of fruits and prolong shelf-life at the same time. In this study, the influence of LPE on anthocyanin accumulation and phytochemical characteristics of sweet cherry was investigated. LPE ($10mg{\cdot}L^{-1}$) was applied to a commercial sweet cherry c.v. '0900 Ziraat' orchard two and four weeks before harvest for two treatment years (2011 and 2012). Preharvest applications of LPE resulted in significant improvement in both pomological and phytochemical attributes at harvest. LPE treatment led to a 17% increase in fruit weight and a 6% increase in soluble solid content when averaged over two experimental years. Fruit phytochemical content and antioxidant capacity were increased significantly. The average total phenolic content of LPE-treated fruits for the two years was $703{\mu}g$ gallic acid equivalent (GAE)/g fresh weight (g FW) compared to $569{\mu}g$ GAE/g FW in the untreated control. Fruits treated with LPE had a 27% and 16% more anthocyanin than the control fruits in 2011 and 2012. Antioxidant capacity of fruits, as measured by TEAC (Trolox equivalent antioxidant capacity) assay, was 12.5 and $11.4{\mu}mol$ TE/g FW in LPE-treated and untreated control fruits, respectively, when averaged over two experimental years. Our results suggest that preharvest application of LPE may have the potential to increase anthocyanin accumulation, improve fruit quality and enhance phytochemical characteristics of sweet cherries.

Lysophosphatidylethanolamine (LPE) Improves Fruit Size, Color, Quality and Phytochemical Contents of Sweet Cherry c.v. ‘0900 Ziraat’

Mustafa Ozgen,Sedat Serce,Ya?ar Akca,Ji Heun Hong 한국원예학회 2015 원예과학기술지 Vol.33 No.2

Lysophosphatidylethanolamine (LPE) affects the quality of flowers, fruits, and other horticultural products. Studies have provided evidence that LPE can accelerate ripening of fruits and prolong shelf-life at the same time. In this study, the influence of LPE on anthocyanin accumulation and phytochemical characteristics of sweet cherry was investigated. LPE (10 ㎎·L<SUP>-1</SUP>) was applied to a commercial sweet cherry c.v. ‘0900 Ziraat’ orchard two and four weeks before harvest for two treatment years (2011 and 2012). Preharvest applications of LPE resulted in significant improvement in both pomological and phytochemical attributes at harvest. LPE treatment led to a 17% increase in fruit weight and a 6% increase in soluble solid content when averaged over two experimental years. Fruit phytochemical content and antioxidant capacity were increased significantly. The average total phenolic content of LPE-treated fruits for the two years was 703 ㎍ gallic acid equivalent (GAE)/g fresh weight (g FW) compared to 569 ㎍ GAE/g FW in the untreated control. Fruits treated with LPE had a 27% and 16% more anthocyanin than the control fruits in 2011 and 2012. Antioxidant capacity of fruits, as measured by TEAC (Trolox equivalent antioxidant capacity) assay, was 12.5 and 11.4 μ㏖ TE/g FW in LPE-treated and untreated control fruits, respectively, when averaged over two experimental years. Our results suggest that preharvest application of LPE may have the potential to increase anthocyanin accumulation, improve fruit quality and enhance phytochemical characteristics of sweet cherries.

Strawberry Runner Tip Production on Open Field for Plug Plants

Emine Ozdemir,Nurettin Kaska,Kazim Gunduz,Sedat Serce 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1

Plug plants are important for early strawberry production and have been replacing commonly used frigo plants. In this study, runner tip production for the production of plug plants was evaluated in a two-year study (2006 and 2007) using the leading cultivars ‘Camarosa’ and ‘Sweet Charlie’ under the Mediterranean conditions in Turkey. The effects of GA₃ applications were also investigated. The frigo plants were planted on hills covered by black plastic mulch on 15 March and the runner tips were evaluated at the end of June. The runner and runner tip numbers, stolon and crown diameters, and runner tip weight were evaluated. Runner tips were statistically different between the two cultivars for both years. ‘Camarosa’ had more runner tips in 2006 (16.4 vs. 13.4), whereas ‘Sweet Charlie’ produced more runner tips in 2007 (19.3 vs. 16.1). The effects of GA3 applications were variable for runner and runner tips. However, in general, GA3 application reduced the stolon and crown diameters and runner tip weight.

Morphological Characterization of Several Morus Species from Turkey

Mustafa Ozgen,Mehmet Gune?,Ya?ar Akca,Nurgul Turemi?,Muruvvet Ilgin,Gokhan Kizilci,Ummugulsum Erdo?an,Sedat Serce 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1

Among edible mulberry (Morus spp.) species, M. alba, M. nigra, M. rubra, and M. laevigata grow in Turkey. Several regions of Turkey were surveyed during the 2007 and 2008 growing seasons. More than 200 promising mulberry accessions were visited and preliminary tree and fruit characteristics were investigated. Based on the first year results, a total of 22 superior accessions from four different species were morphologically characterized in the second year. Considerable variations were observed for most of the traits. For example, fruit weight ranged from 3.3 to 8.2 g for an average of 4.9 g. M. nigra accessions had much smaller mean values when compared to those of M. rubra and M. alba. The juice yield ranged from 56.1 to 67.8% for M. nigra accessions, 20.0 to 31.1% for M. rubra and 25.6 to 35.0% for M. alba. The mean total acidity of M. nigra was 2.7%, 3.1-fold higher than those of M. rubra and M. alba accessions. Soluble solids ranged from 14.1 to 27.1% for an average of 21.8%. These fruit traits were subjected to principle component analysis and the results indicated that M. nigra accessions were grouped together, while M. laevigata accession was distinct from other genotypes. The accessions of M. rubra and M. alba separated from M. nigra and M. laevigata accessions, although the two groups did not separate from each other.