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( Jin Seoub Kim ),( Hye Seon Kim ),( Hee Chul Nam ),( Pil Soo Sung ),( Si Hyun Bae ),( Jong Young Choi ),( Seung Kew Yoon ),( Jeong Won Jang ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Telomerase reverse transcriptase (TERT) was recently identified as a gatekeeper driver gene with a fundamental role in multi-step hepatocarcinogenesis. This study aimed to investigate the tumor-promoting effects of TERT interaction gene set and genomic alterations in hepatocellular carcinoma (HCC). Methods: A total of 239 biopsy-proven HCC samples were analyzed for the study. Protein-protein interaction (PPI) networks through the STRING database were performed to establish a set of interacting genes with TERT. The expression of the selected gene set, TERT, and telomere length were examined by qRT-PCR. TERT promoter mutations were assessed using Sanger sequencing. Integration of hepatitis B virus (HBV) was examined using the NGS-based probe capture assay. The data were correlated with outcomes of HCC patients. Results: The PPI networks identified eight key TERT-interaction gene sets, such as CCT5, TUBA1B, mTOR, RPS6KB1, AKT1, WHAZ, YWHAQ, and TERT. Among these, TERT was the most significant differentially expressed gene, with its significantly higher expression in the tumor than non-tumor tissues. The high expression of TERT was correlated with tumor size and HCC stage progression. TERT expression and telomere lengths were positively correlated in patients with HBV-HCC invading portal vein. Together with telomere length, the presence of C228T, the hot spot mutation in the TERT promoter, as well as HBV integration resulted in a higher expression of TERT, with enhanced expression with the presence of both TERT promoter mutation and HBV integration. Patients with high TERT expression had significantly higher rates of recurrence and worse progression- free survival than those with low TERT expression. Conclusions: TERT gene alterations and expression are involved in hepatocarcinogenesis and the clinical outcomes of HCC, in terms of the development, recurrence, and disease progression. TERT pathway might serve as a potential therapeutic target for HCC.
Young-Seoub Hong,Dae-Seon Kim,Seung-Do Yu,Seong-Hwan Kim,Jong-Kuk Kim,Yu-Mi Kim,Jae-Ho Yu,Ji-Hyun Jung,Byoung-Gwon Kim 대한직업환경의학회 2013 대한직업환경의학회지 Vol.25 No.-
Background: Methylmercury (MeHg) easily crosses the blood?brain barrier and accumulates in the brain. Accumulated MeHg will cause neurological symptoms. We report four pediatric cases of neuropsychological findings with high blood MeHg concentrations. Case presentation: Four children were admitted for follow-up study because their total mercury (THg) concentration in the blood was found to be high during a national survey. Case 1 was a 9-year-old female with a 16.6 μg/ℓ blood THg concentration in the survey. During admission, the blood THg, hair THg, and blood MeHg concentration(mercury indices) were 21.4 μg/ℓ, 7.2 μg/g, and 20.1 μg/ℓ, respectively. In our neuropsychological examination, cognitive impairment and attention deficit were observed. Her diet included fish intake 2?3 times per week, and she had been diagnosed with epilepsy at 3 years of age. Case 2 was a 12-year-old male with blood THg of 15.4 μg/ℓ in the survey and the mercury indices were 12.7 μg/ℓ, 5.7 μg/g, and 11.8 μg/ℓ, respectively, on admission. He was also observed to have attention-deficit/hyperactivity disorder. Case 3 was a 10-year-old male child with blood THg of 17.4 μg/ℓ in the survey, and the mercury indices on admission were 21.6 μg/ℓ, 7.5 μg/g and 21.5 μg/ℓ, respectively. In his case, mild attention deficit was observed. Case 4 was a 9-year-old male with blood THg of 20.6 μg/ℓ in the survey and the mercury indices were 18.9 μg/ℓ, 8.3 μg/g, and 14.4 μg/ℓ, respectively, on admission. Mild attention difficulty was observed. Conclusion: We suggest that fish consumption may be the main source of MeHg exposure, and that MeHg may have been the cause of the neuropsychological deficits in these cases.
DACUM법을 이용한 축산분야 국가기술자격의 시험과목과 출제기준 개발
김규섭(Kyu-Seoub Kim) 한국콘텐츠학회 2012 한국콘텐츠학회논문지 Vol.12 No.7
이 연구는 축산 분야의 구체적인 직무 분석을 통하여 축산분야 자격의 시험과목 및 출제기준을 설계하는데 목적이 있다. 연구 목적을 달성하기 위해서 본 연구에서는 산업계, 학계 전문가들로 구성된 직무분석위원회에서 데이컴(DACUM : Development A curriculum)법으로 직무분석을 실시하여 직무범위, 응시자격, 검정기준, 출제기준, 검정방법 등의 구성 내용을 추출하였다 또한 이 연구에서는 축산 분야의 출제기준 개선을 위한 직무분석의 모형을 절차별로 검토 후 정리?제시하였는데, 1단계는 직무분석 준비(자료조사 및 면담, 전문가 선정), 2단계는 직무모형 설정(1차 전문가 협의회 및 1차 현장 검토)하였고, 3단계는 지식, 기능, 도구 추출(2차 전문가 협의회 및 2차 현장검토), 4단계는 코스 및 시험과목 선정(3차 전문가 협의회), 5단계는 출제기준 설정(3차 현장 검토)의 과정을 통하여 축산 분야 자격의 출제기준을 제시하였다. This study is aimed to design for qualification structure and criteria for preparation of qualification test of the Livestock. In this study, a DACUM(Development A curriculum) method job analysis to extract contents such as task range, eligibility, examination standards, selection criteria of examination questions, examination methods, etc was done by an job analysis committee composed of 7 specialists in the industry and academia. In the first place, this report presents job analysis models by procedures for developing selection criteria of examination questions of the Livestock. The first step is preparation for job analysis(data research, interviews and selection of experts), the second step the establishment of job models(first expert council and site study), the third step the drawn-out of knowledge, skills, and tools(the second expert council and site study), the fourth step the selection of courses and an examination subject(the third expert council), the fifth step the establishment of guidelines for making questions(the third site study).
Hypermethylation of <i>p16<sup>INK4a</sup></i> in Korean Non-small Cell Lung Cancer Patients
Hong, Young-Seoub,Roh, Mee-Sook,Kim, Na-Young,Lee, Hye-Jung,Kim, Hee-Kyoung,Lee, Kyung-Eun,Kwak, Jong-Young,Kim, Joon-Youn KOREAN ACADEMY OF MEDICAL SCIENCE 2007 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.22//SUP No.-
<P>Promoter hypermethylation of the <I>p16<SUP>INK4a</SUP></I> gene was investigated in 81 sets of samples of tumor tissue and adjacent normal tissue from Korean patients with primary lung cancer, using the modified real-time polymerase chain reaction (PCR)/ SYBR Green detection method. The results showed hypermethylation of <I>p16<SUP>INK4a</SUP></I> in 27.2% of tumor tissues, and in 11.1% of adjacent normal tissue. No significant association was found between the overall aberrant methylation in tumor and corresponding normal specimens (r=0.137, <I>p</I>=0.219). In 22 cases with <I>p16<SUP>INK4a</SUP></I> hypermethylation in tumor tissues, only 4 (18.1%) cases were found to have a hypermethylated normal tissue specimen. The findings of this study show that smoking can influence the methylation level of the promoter region of <I>p16<SUP>INK4a</SUP></I>, and that this occurs in tumor tissues more frequently than in normal tissues. Other clinicopathological characteristics, including age, sex, tumor stage, and histologic type were not found to be correlated with <I>p16<SUP>INK4a</SUP></I> methylation.</P>
Hong, Young-Seoub,Kang, Ho-Jin,Kwak, Jong-Young,Park, Byung-Lae,You, Chang-Hun,Kim, Yu-Mi,Kim, Heon The Korean Society for Preventive Medicine 2011 Journal of Preventive Medicine and Public Health Vol.44 No.3
Objectives: The microRNA (miRNA) miR-196a2 may play an important role in lung cancer development and survival by altering binding activity of target mRNA. In this study, we evaluated their associations with the susceptibility of non-small cell lung cancers (NSCLC) by case-control study in a Korean population. Methods: We performed genotyping analyses for miR-196a2 rs11614913 T/C at miRNA regions in a case-control study using blood samples of 406 NSCLC patient and 428 cancer-free control groups. Results: The total C allele frequencies for miR-196a2 were 48.8% for the patients and 45.6% for the controls; and the genotype frequencies of TT, TC, and CC were 23.7%, 55.2%, and 21.1% for the patients and 31.1%, 46.35%, and 22.4% for the controls (p<0.05). Participants who possesses TC/CC genotypes showed high risk for NSCLC compared to those possessed TT genotypes (OR, 1.42; 95% CI, 1.03 to 1.96). The association was persisted in 60 and older age group, male, smokers, those without family history for cancer. However, no significant association of CC genotypes in recessive genetic model was observed. Conclusions: In conclusion, this case-control study provides evidence that miR-196a2 rs11614913 C/T polymorphisms are associated with a significantly increased risk of NSCLC in a dominant model, indicating that common genetic polymorphisms in miR-196a2 rs11614913 are associated with NSCLC. The association of miR196a2 rs11614913 polymorphisms and NSCLC risk require confirmation through additional larger studies.