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( Seong Hee Park ),( Sung Soo Jun ),( Gyn Heung An ),( Young Nam Hong ),( Min Chul Park ) 한국식물학회 2003 Journal of Plant Biology Vol.46 No.4
Trehalose and LEA proteins, representative low MW chemicals that are synthesized under dehydration, are known to protect plants from drought stress. To compare their effectiveness on enhancing tolerance against various abiotic stresses, we generated transgenic Chinese cabbage plants overexpressing E. Coli trehalose-6-phasphate synthase gene (otsA) or hot pepper (Capsicum annuum) LEA protein gene (CaLEA), Both transgenic plants exhibited altered phenotype including stunted growth and aberrant root development. When subjected to drought, salt or heat stress, these plants showed remarkably improved tolerance against those stresses compared with nontransformants. After dehydration treatment, leaf turgidity and fresh weight was better maintained in both transgenic plants. CaLEA-plants performed somewhat better under dehydrated condition. When treated with 250 mM NaCl, both otsA-plants and CaLEA-plants remained equally healthier than nontransformants in maintaining leaf turgidity and delaying necrosis. Furthermore, leaf Chl content and Fv/Fm was maintained considerably higher in both transgenic plants than nontransformants. After heat-treatment at 45℃, both transgenic plants appeared much less damaged in external shape and PSⅡ function, but LEA proteins were more protective. Our results indicate that although both trehalose and LEA proteins are effective in protecting plants against various abiotic stresses, LEA proteins seem to be more promising in generating stress-tolerant transgenic plants.
Seong-Hee Park,Michael Cottingham,Won-Jae Seo 한국유통과학회 2018 The Journal of Industrial Distribution & Business( Vol.9 No.9
Purpose – The current study is to compare the cognition of stakeholders on hosting a mega sports event between Korea and the United States. In particular, to understand their cognition and perceptual conflict towards hosting a mega sports event, the study employed conflict theory. Furthermore, the study reviewed the role of social capital in the process of managing the mega sports events. Research Design, Data, and Methodology – Of homogeneous sampling, purposeful sampling method and criterion-based selection approach were used to collect interview data from key stakeholders who have been involved in hosting a mega sports events in Korea and the United States. In-depth interview transcripts were reviewed multiple tiems after transcription to extract concepts and meanings that were pertinenet to the experience involving hosting a mega sports event. Further member checks was conducted to increase the credibility of the results. Results – Results can be summarized as followed: First, stakeholders of Korea have a strong desire for positive economic effects of a mega sports event, compared to those in the United States who are more concerned in enhancing the public interests and concerns. Second, in Korea, various socio-political issues emerged at the same time and conflicts among multiple stakeholders have aggravated the situations to coordinate the issues. This was because legal system supporting socio-trust has not been established. On the other hand, major stakeholders of the United States consisted of community members who have socio-trust and networks. Thereby these social resources have been found playing a key role in building social capital that assists the stakeholders to coordinate the current issues and to solve them. Conclusions – The current study analyzed the cognition and perceptual conflict of stakehoders in a mega sports event. Social capital has beend found as a key catalyst to increase a network and cooperation among stakeholders. In order to enhance social capital in managing a mega sports event hosted in Korea, legal systems that establish networks and relationships among the related stakeholders need to be developed. Furthermore, the systematic guideline needs to be developed, organizing the sub-committees according to the types of stakeholders and the categorized common needs.
Seong-Hee Park,Jae-Yoen Kim,Hyun-Jeong Kim,Kwang-Kyun Park,Kyoo-Sung Cho,Seong-Ho Choi,Won-Yoon Chung KOREAN ACADAMY OF ORAL BIOLOGY 2008 International Journal of Oral Biology Vol.33 No.4
Gingival overgrowth can cause dental occlusion and seriously interfere with mastication, speech, and dental hygiene. It is observed in 25 to 81 % of renal transplant patients treated with cyclosporine A (CsA). CsA-induced gingival overgrowth (CIGO) is caused by quantitative alteration of the extracellular matrix components, particularly collagen. However, the molecular mechanisms involved in the pathogenesis of CIGO remain poorly understood, despite intense clinical and laboratory investigations. The aim of the present work is to identify differentially expressed genes closely associated with CIGO. Human gingival fibroblasts were isolated by primary explant culture of gingival tissues from five healthy subjects (HGFs) and two patients with the CIGO (CIGO-HGFs). The proliferative activity of CsA-treated HGFs and CIGOHGFs was examined using the MTT assay. The identification of differentially expressed genes in CsAtreated CIGO-HGF was performed by differential display reverse transcriptase-polymerase chain reaction (RT-PCR) followed by DNA sequencing. CsA significantly increased the proliferation of two HGFs and two CIGO-HGFs, whereas three HGFs were not affected. Seven genes, including the beta subunit of prolyl 4-hydroxylase (P4HB) and testican 1, were upregulated by CsA in a highly proliferative CIGO-HGF. The increased P4HB and testican-1 mRNA levels were confirmed in CsA-treated CIGO-HGFs by semiquantitative RT-PCR. Furthermore, CsA increased type I collagen mRNA levels and suppressed MMP-2 mRNA levels, which are regulated by P4HB and testican-1, respectively. These results suggest that CsA may induce gingival overgrowth through the upregulation of P4HB and testican-1, resulting in the accumulation of extracellular matrix components.